In traditional Chinese medicine herbs(TCM),including Radix Salviae Miltiorrhizae(Danshen),Radix Puerariae Lobatae(Gegen),Radix Angelicae Sinensis(Danggui),and Rhizoma Chuanxiong(Chuanxiong)are widely used for the prev...In traditional Chinese medicine herbs(TCM),including Radix Salviae Miltiorrhizae(Danshen),Radix Puerariae Lobatae(Gegen),Radix Angelicae Sinensis(Danggui),and Rhizoma Chuanxiong(Chuanxiong)are widely used for the prevention and treatment of cardiovascular diseases and also often co-administered with Western drugs as a part of integrative medicine practice.Carboxylesterase 1(CES1)plays a pivotal role in the metabolisms of pro-drugs,Since(S)-2-(2-(6-dimethylamino)-benzothiazole)-4,5-dihydrothiazole-4-carboxylate(NLMe)has recently been identified by us as a selective CES1 bioluminescent sensor,we developed a rapid method using this substrate for the direct measurement of CES1 activity in rats.This bioluminescence assay was applied to determine CES1 activity in rat tissues after a two-week oral administration of each of the four herbs noted above.The results demonstrated the presence of CES1 enzyme in rat blood and all tested tissues with much higher enzyme activity in the blood,liver,kidney and heart than that in the small intestine,spleen,lung,pancreas,brain and stomach.In addition,the four herbs showed tissue-specific effects on rat CES1 expression.Based on the CES1 biodistribution and its changes after treatment in rats,the possibility that Danshen,Gegen and Danggui might alter CES1 activities in human blood and kidney should be considered.In summary,a selective and sensitive bioluminescence assay was developed to rapidly evaluate CES1 activity and the effects of orally administered TCMs in rats.展开更多
Objective The metabolic reprogramming of acute myeloid leukemia(AML)cells is a compensatory adaptation to meet energy requirements for rapid proliferation.This study aimed to examine the synergistic effects of glutami...Objective The metabolic reprogramming of acute myeloid leukemia(AML)cells is a compensatory adaptation to meet energy requirements for rapid proliferation.This study aimed to examine the synergistic effects of glutamine deprivation and metformin exposure on AML cells.Methods SKM-1 cells(an AML cell line)were subjected to glutamine deprivation and/or treatment with metformin or bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl)ethyl sulfide(BPTES,a glutaminase inhibitor)or cytarabine.Cell viability was detected by Cell Counting Kit-8(CCK-8)assay,and cell apoptosis and reactive oxygen species(ROS)by flow cytometry.Western blotting was conducted to examine the levels of apoptotic proteins,including cleaved caspase-3 and poly(ADP-ribose)polymerase(PARP).Moreover,the human long noncoding RNA(lncRNA)microarray was used to analyze gene expression after glutamine deprivation,and results were confirmed with quantitative RT-PCR(qRT-PCR).The expression of metallothionein 2A(MT2A)was suppressed using siRNA.Cell growth and apoptosis were further detected by CCK-8 assay and flow cytometry,respectively,in cells with MT2A knockdown.Results Glutamine deprivation or treatment with BPTES inhibited cell growth and induced apoptosis in SKM-1 cells.The lncRNA microarray result showed that the expression of MT family genes was significantly upregulated after glutamine deprivation.MT2A knockdown increased apoptosis,while proliferation was not affected in SKM-1 cells.In addition,metformin inhibited cell growth and induced apoptosis in SKM-1 cells.Both glutamine deprivation and metformin enhanced the sensitivity of SKM-1 cells to cytarabine.Furthermore,the combination of glutamine deprivation with metformin exhibited synergistic antileukemia effects on SKM-1 cells.Conclusion Targeting glutamine metabolism in combination with metformin is a promising new therapeutic strategy for AML.展开更多
Objective:To investigate effects of hemoperfusion and hemofiltration combination on treating patients with acute organophosphours pesticide poisoning (AOPP) and influence of it on cholinesterase, dopamine and inflamma...Objective:To investigate effects of hemoperfusion and hemofiltration combination on treating patients with acute organophosphours pesticide poisoning (AOPP) and influence of it on cholinesterase, dopamine and inflammatory factors.Methods:A total of 82 cases of AOPP patients treated in our hospital from Sep 2012 to Jul 2016 were selected as subjects. They were randomly divided to be the observation group and the control group, 41 cases for each. For patients in observation group, combined therapy of hemoperfusion (HP) and hemofiltration (HF) were provided. For patients in control group, combined therapy of HP and hemodialysis (HD) were provided. Effects on the two groups of patients were compared. Meanwhile, cholinesterase, dopamine and inflammatory factors levels in different times before and after treatment were compared.Results:Consciousness improvement times and hospitalization times in observation group were significantly lower than in control group. No significant difference showed on fatality rates between the two groups. Before treatment, no significant difference showed on CHE and DE levels between two groups of patients;6 h and 12 h after treatment, CHE average levels in two groups were significantly higher than before treatment in the same group, and levels in observation group at the same phase were significantly higher than in control group;6 h and 12 h after treatment, DA levels in observation group were significantly lower than the same group before treatment, and significantly lower than control group, while 12 h after treatment, DA levels in control group were significantly lower than the same group before treatment. Before treatment, no significant difference showed on serum TGF-β1, TNF-α, IL-6, IL-8 between two groups of patients. After treatment, each index levels in two groups were significantly lower than the same group before treatment, and levels in observation group at the same phase were significantly lower than control group. Conclusion:Effects of blood purification therapy on treating AOPP were worth approving, but effects of HP and HF combined therapy were more significant. In addition, improvement of HP+HF on CHE, DA and inflammatory factors were better than HP+HD.展开更多
Two kinds of oxide-zeolite composite support,Ce-beta and Zr-beta were prepared by a simple wet impregnation method and adopted for the preparation of palladium-based catalysts for catalytic oxidation of methane.The Pd...Two kinds of oxide-zeolite composite support,Ce-beta and Zr-beta were prepared by a simple wet impregnation method and adopted for the preparation of palladium-based catalysts for catalytic oxidation of methane.The Pd/6.8Zr-beta catalyst showed superiormethane oxidation performance,achieving T_(50) and T90 of 417℃ and 451℃,respectively,together with robust hydrothermal stability.Kinetic analysis has shown that incorporating Zr into the catalyst significantly enhanced its efficiency,nearly tripling the turnover frequency(TOF)for methane combustion compared to the Pd/beta catalyst.This enhanced performance was attributed to the dispersion of Zr on the zeolite surface,which not only promoted the formation of active PdO sites but also helped maintain the high Pd^(2+)content via facilitating the oxygen migration during the reaction,thus improving both the catalyst’s activity and stability.In the Pd/8.6Ce-beta catalyst,doped CeO_(2) tended to aggregate in the zeolite’s pores,adversely affecting the catalyst’s efficiency.This aggregation promoted the formation of inactive Pd^(4+) species,a result of the enhanced metal-support interaction.This finding is critical for understanding the implications of dopant selection in the design of high-activity methane oxidation catalysts.展开更多
The genetic regulation of hair density in animals remains poorly understood.The Dazu black goat,characterized by its black coarse hair and white skin,provides a unique model for dissecting coarse hair density(CHD).Usi...The genetic regulation of hair density in animals remains poorly understood.The Dazu black goat,characterized by its black coarse hair and white skin,provides a unique model for dissecting coarse hair density(CHD).Using high-resolution micro-camera imaging,this study analyzed 905 skin images,33 skin transcriptomes,272 whole-genome sequences,and 182 downloaded transcriptomes.Morphological assessment from juvenile to adult stages revealed the thickening of hair shafts accompanied by a progressive decline in density,largely attributable to rapid surface expansion of the trunk skin.Transcriptomic comparison between high-and low-CHD individuals identified 572 differentially expressed genes(DEGs).A genome-wide association study detected 25 significant single nucleotide polymorphisms(P<9.07e-8)and mapped 48 annotated genes,with the most prominent association signal located near GJA1 on chr9.15931585-18621011.Literature review and Venn analysis highlighted six genes(GJA1,GPRC5D,CD1D,CD207,TFAM,and CXCL12)with documented roles in skin and hair biology,and three genes(GJA1,GPRC5D,and ATP6V1B1)overlapped with DEGs.Multiple-tissue transcriptomic profiling,western blotting,immunohistochemical staining,and skin single-cell RNA sequencing confirmed that GJA1 and GPRC5D were highly and specifically expressed in skin,particularly within hair follicles.Expression was localized predominantly to follicular stem cells and dermal papilla cells,suggesting a significant role in folliculogenesis and structural maintenance.Cross-validation using four public datasets further demonstrated positive correlations between GJA1 and GPRC5D expression and hair follicle density.The innovative micro-camera application allowed the elucidation of spatiotemporal patterns and genes associated with CHD,thereby addressing a significant knowledge gap in animal hair density.展开更多
Sugarcane bagasse(SCB)is a promising natural fiber for bio-based composites,but its high moisture absorption and poor interfacial adhesion with polymer matrices limit mechanical performance.While chemical treatments h...Sugarcane bagasse(SCB)is a promising natural fiber for bio-based composites,but its high moisture absorption and poor interfacial adhesion with polymer matrices limit mechanical performance.While chemical treatments have been extensively explored,limited research has addressed how thermal treatment alone alters the surface properties and reinforcing behavior of SCB fibers.This study aims to fill that gap by investigating the effects of heat treatment on SCB fiber structure and its performance in starch/poly(vinyl alcohol)(PVA)composites.Characterization techniques including Fourier transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),and scanning electron microscopy(SEM)were employed to analyze changes in fiber morphology,surface chemistry,and crystallinity.Mechanical properties were assessed via tensile,flexural,and impact testing,and moisture absorption was also evaluated.Composites reinforced with SCB fibers treated at 200○C exhibited significantly superior mechanical properties compared to those prepared with untreated or differently treated fibers.The tensile,flexural,and impact performance of the composites were 15.13,19.37 MPa,and 7.28 J/m,respectively.Composites treated at this temperature also retained better mechanical properties after exposure to humidity.These findings demonstrate that heat treatment is a simple and sustainable method to improve the durability and mechanical performance of nature fiber-reinforced composites,expanding their potential for environmentally friendly material applications.展开更多
This study investigates the Maximum Power Point Tracking(MPPT)control method of offshore windphotovoltaic hybrid power generation system with offshore crane-assisted.A new algorithm of Global Fast Integral Sliding Mod...This study investigates the Maximum Power Point Tracking(MPPT)control method of offshore windphotovoltaic hybrid power generation system with offshore crane-assisted.A new algorithm of Global Fast Integral Sliding Mode Control(GFISMC)is proposed based on the tip speed ratio method and sliding mode control.The algorithm uses fast integral sliding mode surface and fuzzy fast switching control items to ensure that the offshore wind power generation system can track the maximum power point quickly and with low jitter.An offshore wind power generation system model is presented to verify the algorithm effect.An offshore off-grid wind-solar hybrid power generation systemis built in MATLAB/Simulink.Compared with other MPPT algorithms,this study has specific quantitative improvements in terms of convergence speed,tracking accuracy or computational efficiency.Finally,the improved algorithm is further analyzed and carried out by using Yuankuan Energy’s ModelingTech semi-physical simulation platform.The results verify the feasibility and effectiveness of the improved algorithm in the offshore wind-solar hybrid power generation system.展开更多
One effective approach to strike the balance between ionic conductivity and mechanical strength in polymer electrolytes involves the design of a coupled polymer molecular structure comprising both rigid and flexible p...One effective approach to strike the balance between ionic conductivity and mechanical strength in polymer electrolytes involves the design of a coupled polymer molecular structure comprising both rigid and flexible phases.Nevertheless,the regulation of intermolecular interactions between plasticizers and rigid and flexible phases has been largely overlooked.Here,an intermolecular interaction engineering strategy is carried out with well-chosen dual-plasticize within qua si-sol id-state polymer electrolytes(QSPEs).Succinonitrile exhibits a stronger affinity towards rigid phase hydrogenated nitrile butadiene rubber(HNBR),while propene carbonate demonstrates a stronger affinity towards flexible segments poly(propylene carbonate)(PPC).This tailored intermolecular interaction engineering allows for differential plasticization of the polymer's rigid and flexible phases,thereby achieving a balance between ionic conductivity and mechanical strength.The QSPE have both higher ionic conductivity(1.04×10^(-4)S cm^(-1)at 30℃),t_(Li+)(0.55),and tensile strength(0.76 MPa).Li//Li symmetric cells maintaining performance over1100 h at 0.1 mA cm^(-2)and Li//LiFePO_(4)cells retaining 85.0%capacity after 700 cycles at 1.0 C.It is a unique angle to employ intermolecular interaction engineering in QSPEs through dual-plasticizer approach combined with CO_(2)-based polymer materials.This sustainable strategy combining dual-plasticizer engineering with CO_(2)-based polymers,offers insights for designing high-performance,eco-friendly lithium metal batteries.展开更多
Acupuncture has been proven effective in treating depressive disorders,and its neuroelectrophysiologicalmechanisms have drawn increasing scientific attention.This paper reviews recent neuroelectrophysiological studies...Acupuncture has been proven effective in treating depressive disorders,and its neuroelectrophysiologicalmechanisms have drawn increasing scientific attention.This paper reviews recent neuroelectrophysiological studies on acupuncture in the treatment of depressive disorders.Studies have shown that acupuncturecan modulate the Alpha and Theta wave frequencies and amplitudes of electroencephalograms;improvethe latency and amplitude of event-related potential such as P300 and mismatch negativity(MMN);enhance the amplitude of motor-evoked potential and short-interval intracortical inhibition as measured bytranscranial magnetic stimulation-electromyography(TMS-EMG);regulate both spontaneous and inducedrhythmic activities;increase motor cortex excitability;and promote the electrical signal transmission inneuronal clusters.Acupuncture can enhance the long-term potentiation(LTP)detected by PCRT,repair theplasticity of synaptic functions,and promote the transmission of synaptic information;lower the actionpotential(AP)and population spike(PS)membrane voltage detected by PCRT,inhibit abnormal neuronalexcitability,and restore electrical signal transmission across neuronal membranes.These findings,as summarized in this review,may reflect potential neuroelectrical signal transmission mechanisms involved inthe acupuncture interventions for depressive disorders.展开更多
Single ion gel polymer electrolyte has the advantages of high Li^(+)conductivity and dendrite mitigation.However,the addition of organic solvent makes the electrolyte flammable,posing serious safety hazards.Herein,we ...Single ion gel polymer electrolyte has the advantages of high Li^(+)conductivity and dendrite mitigation.However,the addition of organic solvent makes the electrolyte flammable,posing serious safety hazards.Herein,we report a flame-retard ant cross-linked sp^(3)boron-based single-ion gel polymer electrolyte(BSIPE).BSIPE was prepared by a simple one-step photoinitiated in situ thiol-ene click reaction.Due to the boron-based anions being immobilized in the cross-linking network,the developed BSIPE/PFN exhibits a high t_(Li^(+))(0.87),which can mitigate concentration polarization phenomenon and suppress the growth of lithium dendrites.BSIPE/PFN plasticized with triethyl phosphate(TEP),fluoroethylene carbonate(FEC)and LiNO_(3)exhibits enhanced ionic conductivity of 4.25×10^(-4)S cm^(-1)at 30℃ and flame retardancy.FEC and LiNO_(3) are conducive to form a stable solid electrolyte interphase(SEI)rich in Li_(3)N and LiF to improve interface stability.As expected,the dendrite-free Li‖BSIPE/PFN‖Li symmetric cell exhibits considerable cycling life over 1500 h.BSIPE/PFN significantly boosts the performance of LFP‖Li cell,which displays a capacity retention of 84.6%after 500 cycles.The BSIPE/PFN has promising applications in highsafety and high-performance lithium metal batteries.展开更多
To the Editor:Common gallbladder diseases include gallstones,acute acalculous cholecystitis,chronic cholecystitis,functional gallbladder disorders,gallbladder polyps,gallbladder adenomyomatosis,and gallbladder cancer....To the Editor:Common gallbladder diseases include gallstones,acute acalculous cholecystitis,chronic cholecystitis,functional gallbladder disorders,gallbladder polyps,gallbladder adenomyomatosis,and gallbladder cancer.Laparoscopic cholecystectomy(LC)is the preferred treatment for gallstones with accompanying acute cholecystitis(AC),acute acalculous cholecystitis,chronic cholecystitis,gallbladder polyps(>10 mm),and gallbladder adenomyomatosis[1,2].In 1882,Professor Lamgenbuch completed the first cholecystectomy and placed a drain in the abdominal cavity as part of the surgical procedure[3].The placement of a drainage tube after cholecystectomy used to be a routine procedure.展开更多
基金supported by Health and Medical Research Fund(Reference No.:12131521)from Food and Health Bureau,the Government of the Hong Kong SAR,Hong Kong,ChinaNational Natural Science Foundation of China(Grant No.:81973286,81922070,81703604 and 81973393),ChinaGeneral Research Fund(CUHK2141142)from University Grant Council of Hong Kong SAR,China。
文摘In traditional Chinese medicine herbs(TCM),including Radix Salviae Miltiorrhizae(Danshen),Radix Puerariae Lobatae(Gegen),Radix Angelicae Sinensis(Danggui),and Rhizoma Chuanxiong(Chuanxiong)are widely used for the prevention and treatment of cardiovascular diseases and also often co-administered with Western drugs as a part of integrative medicine practice.Carboxylesterase 1(CES1)plays a pivotal role in the metabolisms of pro-drugs,Since(S)-2-(2-(6-dimethylamino)-benzothiazole)-4,5-dihydrothiazole-4-carboxylate(NLMe)has recently been identified by us as a selective CES1 bioluminescent sensor,we developed a rapid method using this substrate for the direct measurement of CES1 activity in rats.This bioluminescence assay was applied to determine CES1 activity in rat tissues after a two-week oral administration of each of the four herbs noted above.The results demonstrated the presence of CES1 enzyme in rat blood and all tested tissues with much higher enzyme activity in the blood,liver,kidney and heart than that in the small intestine,spleen,lung,pancreas,brain and stomach.In addition,the four herbs showed tissue-specific effects on rat CES1 expression.Based on the CES1 biodistribution and its changes after treatment in rats,the possibility that Danshen,Gegen and Danggui might alter CES1 activities in human blood and kidney should be considered.In summary,a selective and sensitive bioluminescence assay was developed to rapidly evaluate CES1 activity and the effects of orally administered TCMs in rats.
基金supported by the National Natural Science Foundation of China(No.82270177).
文摘Objective The metabolic reprogramming of acute myeloid leukemia(AML)cells is a compensatory adaptation to meet energy requirements for rapid proliferation.This study aimed to examine the synergistic effects of glutamine deprivation and metformin exposure on AML cells.Methods SKM-1 cells(an AML cell line)were subjected to glutamine deprivation and/or treatment with metformin or bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl)ethyl sulfide(BPTES,a glutaminase inhibitor)or cytarabine.Cell viability was detected by Cell Counting Kit-8(CCK-8)assay,and cell apoptosis and reactive oxygen species(ROS)by flow cytometry.Western blotting was conducted to examine the levels of apoptotic proteins,including cleaved caspase-3 and poly(ADP-ribose)polymerase(PARP).Moreover,the human long noncoding RNA(lncRNA)microarray was used to analyze gene expression after glutamine deprivation,and results were confirmed with quantitative RT-PCR(qRT-PCR).The expression of metallothionein 2A(MT2A)was suppressed using siRNA.Cell growth and apoptosis were further detected by CCK-8 assay and flow cytometry,respectively,in cells with MT2A knockdown.Results Glutamine deprivation or treatment with BPTES inhibited cell growth and induced apoptosis in SKM-1 cells.The lncRNA microarray result showed that the expression of MT family genes was significantly upregulated after glutamine deprivation.MT2A knockdown increased apoptosis,while proliferation was not affected in SKM-1 cells.In addition,metformin inhibited cell growth and induced apoptosis in SKM-1 cells.Both glutamine deprivation and metformin enhanced the sensitivity of SKM-1 cells to cytarabine.Furthermore,the combination of glutamine deprivation with metformin exhibited synergistic antileukemia effects on SKM-1 cells.Conclusion Targeting glutamine metabolism in combination with metformin is a promising new therapeutic strategy for AML.
文摘Objective:To investigate effects of hemoperfusion and hemofiltration combination on treating patients with acute organophosphours pesticide poisoning (AOPP) and influence of it on cholinesterase, dopamine and inflammatory factors.Methods:A total of 82 cases of AOPP patients treated in our hospital from Sep 2012 to Jul 2016 were selected as subjects. They were randomly divided to be the observation group and the control group, 41 cases for each. For patients in observation group, combined therapy of hemoperfusion (HP) and hemofiltration (HF) were provided. For patients in control group, combined therapy of HP and hemodialysis (HD) were provided. Effects on the two groups of patients were compared. Meanwhile, cholinesterase, dopamine and inflammatory factors levels in different times before and after treatment were compared.Results:Consciousness improvement times and hospitalization times in observation group were significantly lower than in control group. No significant difference showed on fatality rates between the two groups. Before treatment, no significant difference showed on CHE and DE levels between two groups of patients;6 h and 12 h after treatment, CHE average levels in two groups were significantly higher than before treatment in the same group, and levels in observation group at the same phase were significantly higher than in control group;6 h and 12 h after treatment, DA levels in observation group were significantly lower than the same group before treatment, and significantly lower than control group, while 12 h after treatment, DA levels in control group were significantly lower than the same group before treatment. Before treatment, no significant difference showed on serum TGF-β1, TNF-α, IL-6, IL-8 between two groups of patients. After treatment, each index levels in two groups were significantly lower than the same group before treatment, and levels in observation group at the same phase were significantly lower than control group. Conclusion:Effects of blood purification therapy on treating AOPP were worth approving, but effects of HP and HF combined therapy were more significant. In addition, improvement of HP+HF on CHE, DA and inflammatory factors were better than HP+HD.
基金supported by the National Natural Science Foundation of China(Nos.U20B6004 and 22072179)。
文摘Two kinds of oxide-zeolite composite support,Ce-beta and Zr-beta were prepared by a simple wet impregnation method and adopted for the preparation of palladium-based catalysts for catalytic oxidation of methane.The Pd/6.8Zr-beta catalyst showed superiormethane oxidation performance,achieving T_(50) and T90 of 417℃ and 451℃,respectively,together with robust hydrothermal stability.Kinetic analysis has shown that incorporating Zr into the catalyst significantly enhanced its efficiency,nearly tripling the turnover frequency(TOF)for methane combustion compared to the Pd/beta catalyst.This enhanced performance was attributed to the dispersion of Zr on the zeolite surface,which not only promoted the formation of active PdO sites but also helped maintain the high Pd^(2+)content via facilitating the oxygen migration during the reaction,thus improving both the catalyst’s activity and stability.In the Pd/8.6Ce-beta catalyst,doped CeO_(2) tended to aggregate in the zeolite’s pores,adversely affecting the catalyst’s efficiency.This aggregation promoted the formation of inactive Pd^(4+) species,a result of the enhanced metal-support interaction.This finding is critical for understanding the implications of dopant selection in the design of high-activity methane oxidation catalysts.
基金supported by the National Key Research and Development Program of China(2022YFD1300202)Collection,Utilization,and Innovation of Animal Resources by Research Institutes and Enterprises of Chongqing(Cqnyncw-kqlhtxm),Chongqing Modern Agricultural Industry Technology System(CQMAITS202413)National Training Program of Innovation and Entrepreneurship for Undergraduates(S202310635040)。
文摘The genetic regulation of hair density in animals remains poorly understood.The Dazu black goat,characterized by its black coarse hair and white skin,provides a unique model for dissecting coarse hair density(CHD).Using high-resolution micro-camera imaging,this study analyzed 905 skin images,33 skin transcriptomes,272 whole-genome sequences,and 182 downloaded transcriptomes.Morphological assessment from juvenile to adult stages revealed the thickening of hair shafts accompanied by a progressive decline in density,largely attributable to rapid surface expansion of the trunk skin.Transcriptomic comparison between high-and low-CHD individuals identified 572 differentially expressed genes(DEGs).A genome-wide association study detected 25 significant single nucleotide polymorphisms(P<9.07e-8)and mapped 48 annotated genes,with the most prominent association signal located near GJA1 on chr9.15931585-18621011.Literature review and Venn analysis highlighted six genes(GJA1,GPRC5D,CD1D,CD207,TFAM,and CXCL12)with documented roles in skin and hair biology,and three genes(GJA1,GPRC5D,and ATP6V1B1)overlapped with DEGs.Multiple-tissue transcriptomic profiling,western blotting,immunohistochemical staining,and skin single-cell RNA sequencing confirmed that GJA1 and GPRC5D were highly and specifically expressed in skin,particularly within hair follicles.Expression was localized predominantly to follicular stem cells and dermal papilla cells,suggesting a significant role in folliculogenesis and structural maintenance.Cross-validation using four public datasets further demonstrated positive correlations between GJA1 and GPRC5D expression and hair follicle density.The innovative micro-camera application allowed the elucidation of spatiotemporal patterns and genes associated with CHD,thereby addressing a significant knowledge gap in animal hair density.
文摘Sugarcane bagasse(SCB)is a promising natural fiber for bio-based composites,but its high moisture absorption and poor interfacial adhesion with polymer matrices limit mechanical performance.While chemical treatments have been extensively explored,limited research has addressed how thermal treatment alone alters the surface properties and reinforcing behavior of SCB fibers.This study aims to fill that gap by investigating the effects of heat treatment on SCB fiber structure and its performance in starch/poly(vinyl alcohol)(PVA)composites.Characterization techniques including Fourier transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),and scanning electron microscopy(SEM)were employed to analyze changes in fiber morphology,surface chemistry,and crystallinity.Mechanical properties were assessed via tensile,flexural,and impact testing,and moisture absorption was also evaluated.Composites reinforced with SCB fibers treated at 200○C exhibited significantly superior mechanical properties compared to those prepared with untreated or differently treated fibers.The tensile,flexural,and impact performance of the composites were 15.13,19.37 MPa,and 7.28 J/m,respectively.Composites treated at this temperature also retained better mechanical properties after exposure to humidity.These findings demonstrate that heat treatment is a simple and sustainable method to improve the durability and mechanical performance of nature fiber-reinforced composites,expanding their potential for environmentally friendly material applications.
基金supported by the 2022 Sanya Science and Technology Innovation Project,China(No.2022KJCX03)the Sanya Science and Education Innovation Park,Wuhan University of Technology,China(Grant No.2022KF0028)the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City,China(Grant No.2021JJLH0036).
文摘This study investigates the Maximum Power Point Tracking(MPPT)control method of offshore windphotovoltaic hybrid power generation system with offshore crane-assisted.A new algorithm of Global Fast Integral Sliding Mode Control(GFISMC)is proposed based on the tip speed ratio method and sliding mode control.The algorithm uses fast integral sliding mode surface and fuzzy fast switching control items to ensure that the offshore wind power generation system can track the maximum power point quickly and with low jitter.An offshore wind power generation system model is presented to verify the algorithm effect.An offshore off-grid wind-solar hybrid power generation systemis built in MATLAB/Simulink.Compared with other MPPT algorithms,this study has specific quantitative improvements in terms of convergence speed,tracking accuracy or computational efficiency.Finally,the improved algorithm is further analyzed and carried out by using Yuankuan Energy’s ModelingTech semi-physical simulation platform.The results verify the feasibility and effectiveness of the improved algorithm in the offshore wind-solar hybrid power generation system.
基金supported by the National Key Research and Development Program(2019YFA0705701)National Natural Science Foundation of China(22075329,22008267,21978332 and 22179149)+1 种基金Research and Development Project of Henan Academy Sciences China(232018002)Guangdong Basic and Applied Basic Research Foundation(2021A1515010731)。
文摘One effective approach to strike the balance between ionic conductivity and mechanical strength in polymer electrolytes involves the design of a coupled polymer molecular structure comprising both rigid and flexible phases.Nevertheless,the regulation of intermolecular interactions between plasticizers and rigid and flexible phases has been largely overlooked.Here,an intermolecular interaction engineering strategy is carried out with well-chosen dual-plasticize within qua si-sol id-state polymer electrolytes(QSPEs).Succinonitrile exhibits a stronger affinity towards rigid phase hydrogenated nitrile butadiene rubber(HNBR),while propene carbonate demonstrates a stronger affinity towards flexible segments poly(propylene carbonate)(PPC).This tailored intermolecular interaction engineering allows for differential plasticization of the polymer's rigid and flexible phases,thereby achieving a balance between ionic conductivity and mechanical strength.The QSPE have both higher ionic conductivity(1.04×10^(-4)S cm^(-1)at 30℃),t_(Li+)(0.55),and tensile strength(0.76 MPa).Li//Li symmetric cells maintaining performance over1100 h at 0.1 mA cm^(-2)and Li//LiFePO_(4)cells retaining 85.0%capacity after 700 cycles at 1.0 C.It is a unique angle to employ intermolecular interaction engineering in QSPEs through dual-plasticizer approach combined with CO_(2)-based polymer materials.This sustainable strategy combining dual-plasticizer engineering with CO_(2)-based polymers,offers insights for designing high-performance,eco-friendly lithium metal batteries.
基金Supported by National Natural Science Foundation of China:82360974Open Fund project of Gansu Clinical Medical Center of Acupuncture-Moxibustion and Tuina:ZJKF2204+1 种基金2023 Gansu Provincial University Young Doctor Support Program:2023QB-088Talent Introduction and Research Start-up Fund Project of Gansu University of Chinese Medicine:2023YJRC-01。
文摘Acupuncture has been proven effective in treating depressive disorders,and its neuroelectrophysiologicalmechanisms have drawn increasing scientific attention.This paper reviews recent neuroelectrophysiological studies on acupuncture in the treatment of depressive disorders.Studies have shown that acupuncturecan modulate the Alpha and Theta wave frequencies and amplitudes of electroencephalograms;improvethe latency and amplitude of event-related potential such as P300 and mismatch negativity(MMN);enhance the amplitude of motor-evoked potential and short-interval intracortical inhibition as measured bytranscranial magnetic stimulation-electromyography(TMS-EMG);regulate both spontaneous and inducedrhythmic activities;increase motor cortex excitability;and promote the electrical signal transmission inneuronal clusters.Acupuncture can enhance the long-term potentiation(LTP)detected by PCRT,repair theplasticity of synaptic functions,and promote the transmission of synaptic information;lower the actionpotential(AP)and population spike(PS)membrane voltage detected by PCRT,inhibit abnormal neuronalexcitability,and restore electrical signal transmission across neuronal membranes.These findings,as summarized in this review,may reflect potential neuroelectrical signal transmission mechanisms involved inthe acupuncture interventions for depressive disorders.
基金supported by the National Natural Science Foundation of China(22179149,22075329,51573215,and 21978332)Research and Development Project of Henan Academy Sciences China(232018002)。
文摘Single ion gel polymer electrolyte has the advantages of high Li^(+)conductivity and dendrite mitigation.However,the addition of organic solvent makes the electrolyte flammable,posing serious safety hazards.Herein,we report a flame-retard ant cross-linked sp^(3)boron-based single-ion gel polymer electrolyte(BSIPE).BSIPE was prepared by a simple one-step photoinitiated in situ thiol-ene click reaction.Due to the boron-based anions being immobilized in the cross-linking network,the developed BSIPE/PFN exhibits a high t_(Li^(+))(0.87),which can mitigate concentration polarization phenomenon and suppress the growth of lithium dendrites.BSIPE/PFN plasticized with triethyl phosphate(TEP),fluoroethylene carbonate(FEC)and LiNO_(3)exhibits enhanced ionic conductivity of 4.25×10^(-4)S cm^(-1)at 30℃ and flame retardancy.FEC and LiNO_(3) are conducive to form a stable solid electrolyte interphase(SEI)rich in Li_(3)N and LiF to improve interface stability.As expected,the dendrite-free Li‖BSIPE/PFN‖Li symmetric cell exhibits considerable cycling life over 1500 h.BSIPE/PFN significantly boosts the performance of LFP‖Li cell,which displays a capacity retention of 84.6%after 500 cycles.The BSIPE/PFN has promising applications in highsafety and high-performance lithium metal batteries.
基金supported by grants from the National Key Re-search and Development Program of China(2018YFC2000500)Research Project of Jinan Microecological Biomedicine Shandong Laboratory(JNL-2022022C)。
文摘To the Editor:Common gallbladder diseases include gallstones,acute acalculous cholecystitis,chronic cholecystitis,functional gallbladder disorders,gallbladder polyps,gallbladder adenomyomatosis,and gallbladder cancer.Laparoscopic cholecystectomy(LC)is the preferred treatment for gallstones with accompanying acute cholecystitis(AC),acute acalculous cholecystitis,chronic cholecystitis,gallbladder polyps(>10 mm),and gallbladder adenomyomatosis[1,2].In 1882,Professor Lamgenbuch completed the first cholecystectomy and placed a drain in the abdominal cavity as part of the surgical procedure[3].The placement of a drainage tube after cholecystectomy used to be a routine procedure.
