Manganese residue,including electrolytic manganese residue(EMR)and electrolytic manganese dioxide residue(EMDR),have complicated compositions and are easily generated heavy metals(HMs)and ammonia nitrogen(NH_(4)^(+)-N...Manganese residue,including electrolytic manganese residue(EMR)and electrolytic manganese dioxide residue(EMDR),have complicated compositions and are easily generated heavy metals(HMs)and ammonia nitrogen(NH_(4)^(+)-N)pollution.In this study,HMs and NH_(4)^(+)-N in manganese residue were stabilized and solidified using a combined remediation system of quicklime(CaO),carbon dioxide(CO_(2)),and manganese mineralizing microbes.The sequencing of the manganese mineralizing microbes assemblage revealed that Pseudomonas geniculata,Leclercia adecaarboxylata,Ochrobactrum spp.,and Delftia tsuruhatensis comprised the majority of the assemblage species.Moreover,the manganese mineralizing microbes that were identified by screening and isolation exhibited significant metal resistance,metal absorption,and metal detoxifying abilities according to KEGG pathway level 2 analysis.The NH_(4)^(+)-N levels in the manganese residue across all treatments exhibited a downward trend,meanwhile NH_(4)^(+)-N in the manganese mineralizing microbes treatment groups were all lower than the GB standard(GB8979–1996).The findings of the experiment indicated that when manganese mineralizing microbes(1%M),0.2 L/min CO_(2) flow,and a carbonization time of>15 min were added,the greatest effect of the addition of the innocuous manganese residue was obtained.The innocuous manganese residue exhibited agglomeration and may be utilized as a filler material,according to the XRD,SEM,and EDS results.In this study,manganese mineralizing microbes,CaO and CO_(2) treated HMs and NH_(4)^(+)-N in manganese residue,achieving general industrial solid waste standards,demonstrating potential for large-scale harmless treatment.展开更多
Flavonoids are structurally diverse and the most ubiquitous groups of polyphenols distributed in the various plants,which possess intensive biological activities.In this study,the interaction mechanisms between four f...Flavonoids are structurally diverse and the most ubiquitous groups of polyphenols distributed in the various plants,which possess intensive biological activities.In this study,the interaction mechanisms between four flavonoids containing one glucose unit with similar molecular weight isolated from the Tibetan medicinal herb Pyrethrum tatsienense,namely.apigenin-7-O-β-D-glucoside（1）,luteolin-7-O-β-D-glucoside（2）.quercetin-7-O-β-D-glucoside（3）.quercetin-3-O-β-D-glycoside（4）.and human serum albumin（HSA）,were investigated by fluorescence.UV-vis absorbance,circular dichroism,and molecular modeling.The effects of biological metal ions Mg2＋,Zn2＋,and Cu2＋ on the binding affinity between flavonoids and HSA were further examined.Structure-activity relationships of four flavonoids binding to HSA were discussed in depth and some meaningful conclusions have been drawn by the experiment data and theoretical simulation.In addition,an interesting phenomenon was observed that the microenvironment of the binding site I in HSA has hardly changed in the presence of 4 differentiating from the other three flavonoids on the basis of conformation investigations.展开更多
Enamel demineralization,the formation of white spot lesions,is a common issue in clinical orthodontic treatment.The appearance of white spot lesions not only affects the texture and health of dental hard tissues but a...Enamel demineralization,the formation of white spot lesions,is a common issue in clinical orthodontic treatment.The appearance of white spot lesions not only affects the texture and health of dental hard tissues but also impacts the health and aesthetics of teeth after orthodontic treatment.The prevention,diagnosis,and treatment of white spot lesions that occur throughout the orthodontic treatment process involve multiple dental specialties.This expert consensus will focus on providing guiding opinions on the management and prevention of white spot lesions during orthodontic treatment,advocating for proactive prevention,early detection,timely treatment,scientific follow-up,and multidisciplinary management of white spot lesions throughout the orthodontic process,thereby maintaining the dental health of patients during orthodontic treatment.展开更多
In-depth knowledge of the microbes responsible for biogenic amine(BA)production during soy sauce fermentation remains limited.Herein,the variations in the BA profiles,microbial communities,and microbes involved in BA ...In-depth knowledge of the microbes responsible for biogenic amine(BA)production during soy sauce fermentation remains limited.Herein,the variations in the BA profiles,microbial communities,and microbes involved in BA production during the fermentation of soy sauce through Japanese-type(JP)and Cantonese-type(CP)processes were compared.BA analysis revealed that the most abundant BA species were putrescine,tyramine,and histamine in the later three stages(1187.68,785.16,and 193.20 mg/kg on average,respectively).The BA profiles differed significantly,with CP samples containing higher contents of putrescine,tyramine,and histamine(P<0.05)at the end of fermentation.Metagenomic analysis indicated that BA-producing genes exhibited different abundance profiles,with most genes,including spe A,spe B,arg,spe E,and tyr DC,having higher abundances in microbial communities during the CP process.In total,15 high-quality metagenome-assembled genomes(MAGs)were retrieved,of which 10 encoded at BA production-related genes.Enterococcus faecium(MAG10)and Weissella paramesenteroides(MAG5)might be the major tyramine producers.The high putrescine content in CP might be associated with the high abundance of Staphylococcus gallinarum(MAG8).This study provides a comprehensive understanding of the diversity and abundance of genes involved in BA synthesis,especially at the species level,during food fermentation.展开更多
Endoscopic polypectomy(EP)is the preferred method for the treatment of rectal polyps because of its micro-trauma and rapid recovery.However,the incidence of postoperative abdominal distension can reach 15–30%.This co...Endoscopic polypectomy(EP)is the preferred method for the treatment of rectal polyps because of its micro-trauma and rapid recovery.However,the incidence of postoperative abdominal distension can reach 15–30%.This common complication seriously affects the quality of life of patients after surgery,prolongs the length of hospital stay,and may even be complicated by perforation and other problems.This article systematically reviews the latest research progress on the mechanism,related influencing factors and targeted nursing interventions of abdominal distension after EP,so as to explore effective interventions for postoperative comprehensive nursing,which has core value for improving the postoperative experience and prognosis of EP patients.展开更多
基金supported by the National Natural Science Foundation of China(No.42177392)the Dean’s Research Fund(Nos.2022-23,ref:IRS-4+3 种基金2023/24,ref:IRS-6)of the Education University of Hong KongHunan Provincial Graduate Student Innovation Program(No.CX20240276)Central South University Graduate School-Enterprise Joint Innovation Project(No.2024XQLH011)supported by microbial sequencing from Novomore Biotechnology,Changsha,China.
文摘Manganese residue,including electrolytic manganese residue(EMR)and electrolytic manganese dioxide residue(EMDR),have complicated compositions and are easily generated heavy metals(HMs)and ammonia nitrogen(NH_(4)^(+)-N)pollution.In this study,HMs and NH_(4)^(+)-N in manganese residue were stabilized and solidified using a combined remediation system of quicklime(CaO),carbon dioxide(CO_(2)),and manganese mineralizing microbes.The sequencing of the manganese mineralizing microbes assemblage revealed that Pseudomonas geniculata,Leclercia adecaarboxylata,Ochrobactrum spp.,and Delftia tsuruhatensis comprised the majority of the assemblage species.Moreover,the manganese mineralizing microbes that were identified by screening and isolation exhibited significant metal resistance,metal absorption,and metal detoxifying abilities according to KEGG pathway level 2 analysis.The NH_(4)^(+)-N levels in the manganese residue across all treatments exhibited a downward trend,meanwhile NH_(4)^(+)-N in the manganese mineralizing microbes treatment groups were all lower than the GB standard(GB8979–1996).The findings of the experiment indicated that when manganese mineralizing microbes(1%M),0.2 L/min CO_(2) flow,and a carbonization time of>15 min were added,the greatest effect of the addition of the innocuous manganese residue was obtained.The innocuous manganese residue exhibited agglomeration and may be utilized as a filler material,according to the XRD,SEM,and EDS results.In this study,manganese mineralizing microbes,CaO and CO_(2) treated HMs and NH_(4)^(+)-N in manganese residue,achieving general industrial solid waste standards,demonstrating potential for large-scale harmless treatment.
基金financially supported by the National Natural Science Foundation of China(30960530)
文摘Flavonoids are structurally diverse and the most ubiquitous groups of polyphenols distributed in the various plants,which possess intensive biological activities.In this study,the interaction mechanisms between four flavonoids containing one glucose unit with similar molecular weight isolated from the Tibetan medicinal herb Pyrethrum tatsienense,namely.apigenin-7-O-β-D-glucoside（1）,luteolin-7-O-β-D-glucoside（2）.quercetin-7-O-β-D-glucoside（3）.quercetin-3-O-β-D-glycoside（4）.and human serum albumin（HSA）,were investigated by fluorescence.UV-vis absorbance,circular dichroism,and molecular modeling.The effects of biological metal ions Mg2＋,Zn2＋,and Cu2＋ on the binding affinity between flavonoids and HSA were further examined.Structure-activity relationships of four flavonoids binding to HSA were discussed in depth and some meaningful conclusions have been drawn by the experiment data and theoretical simulation.In addition,an interesting phenomenon was observed that the microenvironment of the binding site I in HSA has hardly changed in the presence of 4 differentiating from the other three flavonoids on the basis of conformation investigations.
基金funded with National Key R&D Program of China(2022YFC2405904)National Natural Science Foundation of China(11932012,and 32171348).
文摘Enamel demineralization,the formation of white spot lesions,is a common issue in clinical orthodontic treatment.The appearance of white spot lesions not only affects the texture and health of dental hard tissues but also impacts the health and aesthetics of teeth after orthodontic treatment.The prevention,diagnosis,and treatment of white spot lesions that occur throughout the orthodontic treatment process involve multiple dental specialties.This expert consensus will focus on providing guiding opinions on the management and prevention of white spot lesions during orthodontic treatment,advocating for proactive prevention,early detection,timely treatment,scientific follow-up,and multidisciplinary management of white spot lesions throughout the orthodontic process,thereby maintaining the dental health of patients during orthodontic treatment.
基金supported by the Natural Science Foundation of Guangdong Province(2022A1515012158)the National Science Foundation of China(41977138)+3 种基金the Construction Project of Teaching Quality and Teaching Reform in Guangdong Province(SJD202001)the General University Project of Guangdong Provincial Department of Education(2021KCXTD070 and 2021ZDZX4072)the Key Project of Social Welfare and Basic Research of Zhongshan City(2020B2010)the Start-up Fund from the Zhongshan Institute at the University of Electronic Science and Technology in China(419YKQN12)。
文摘In-depth knowledge of the microbes responsible for biogenic amine(BA)production during soy sauce fermentation remains limited.Herein,the variations in the BA profiles,microbial communities,and microbes involved in BA production during the fermentation of soy sauce through Japanese-type(JP)and Cantonese-type(CP)processes were compared.BA analysis revealed that the most abundant BA species were putrescine,tyramine,and histamine in the later three stages(1187.68,785.16,and 193.20 mg/kg on average,respectively).The BA profiles differed significantly,with CP samples containing higher contents of putrescine,tyramine,and histamine(P<0.05)at the end of fermentation.Metagenomic analysis indicated that BA-producing genes exhibited different abundance profiles,with most genes,including spe A,spe B,arg,spe E,and tyr DC,having higher abundances in microbial communities during the CP process.In total,15 high-quality metagenome-assembled genomes(MAGs)were retrieved,of which 10 encoded at BA production-related genes.Enterococcus faecium(MAG10)and Weissella paramesenteroides(MAG5)might be the major tyramine producers.The high putrescine content in CP might be associated with the high abundance of Staphylococcus gallinarum(MAG8).This study provides a comprehensive understanding of the diversity and abundance of genes involved in BA synthesis,especially at the species level,during food fermentation.
文摘Endoscopic polypectomy(EP)is the preferred method for the treatment of rectal polyps because of its micro-trauma and rapid recovery.However,the incidence of postoperative abdominal distension can reach 15–30%.This common complication seriously affects the quality of life of patients after surgery,prolongs the length of hospital stay,and may even be complicated by perforation and other problems.This article systematically reviews the latest research progress on the mechanism,related influencing factors and targeted nursing interventions of abdominal distension after EP,so as to explore effective interventions for postoperative comprehensive nursing,which has core value for improving the postoperative experience and prognosis of EP patients.
