目的分析比较盾叶薯蓣根茎与叶片的转录组,挖掘与盾叶薯蓣中皂苷元生物合成途径相关的关键酶基因。方法利用Illumina Hi Seq2000高通量测序技术对盾叶薯蓣根茎与叶片进行转录组测序,对测序结果进行注释分析;并结合根茎与叶片中皂苷元的...目的分析比较盾叶薯蓣根茎与叶片的转录组,挖掘与盾叶薯蓣中皂苷元生物合成途径相关的关键酶基因。方法利用Illumina Hi Seq2000高通量测序技术对盾叶薯蓣根茎与叶片进行转录组测序,对测序结果进行注释分析;并结合根茎与叶片中皂苷元的含量测定结果,挖掘与盾叶薯蓣中皂苷元生物合成途径相关的关键酶基因;最后利用实时荧光定量PCR(q RT-PCR)技术对部分候选基因的表达模式进行分析。结果共获得了81 660个Unigene,有64.33%在NT、NR、Swiss-Prot、KOG、GO、KEGG数据库中得到注释;根据其表达量与代谢通路分析筛选出29种共227个可能参与盾叶薯蓣中皂苷元生物合成途径的催化酶基因,部分催化酶基因的表达模式与皂苷元含量具有一定相关性;并发现5个盾叶薯蓣内生菌基因。结论研究初步获得了盾叶薯蓣中参与皂苷元生物合成的候选关键酶基因,部分候选酶基因可能参与盾叶薯蓣中皂苷类成分的后修饰过程,并发现盾叶薯蓣根茎中内生菌可能参与其皂苷元的生物合成,为进一步阐明盾叶薯蓣中皂苷元生物合成的分子机制奠定基础。展开更多
Metal-insulator-semiconductor back contact has been employed for a perovskite organic lead iodide heterojunction solar cell,in which an ultrathin Al_(2)O_(3) film as an insulating layer was deposited onto the CH_(3)NH...Metal-insulator-semiconductor back contact has been employed for a perovskite organic lead iodide heterojunction solar cell,in which an ultrathin Al_(2)O_(3) film as an insulating layer was deposited onto the CH_(3)NH_(3)PbI_(3) by atomic layer deposition technology.The light-to-electricity conversion efficiency of the devices is significantly enhanced from 3.30%to 5.07%.Further the impedance spectrum reveals that this insulating layer sustains part of the positive bias applied in the absorber region close to the back contact and decreases the carrier transport barrier,thus promoting transportation of carriers.展开更多
The influence of muffin tin approximation on energy band gap was studied using LMTO ASA ( Linear Muffin Tin Orbital Atomic Sphere Approximation) approach. Since the diverse data are available for LaX(X=N, P, As, ...The influence of muffin tin approximation on energy band gap was studied using LMTO ASA ( Linear Muffin Tin Orbital Atomic Sphere Approximation) approach. Since the diverse data are available for LaX(X=N, P, As, Sb), they are presented in our research as an example in order to test the reliability of our results. Four groups of muffin tin radii were chosen, they were the fitted muffin tin radii based on the optical properties of the crystals (the first), 1∶1 for La∶X(the second), 1 5∶1 for La∶X(the third), and a group of radii derived by making the charge in the interstitial space to be zero(the fourth). The results show that the fitted muffin tin radii (the first group) give the best results compared with experimental values, and the predicted energy band gaps are very sensitive to the choice of muffin tin radius in comparison with the other groups. The second and the third delivered results somewhere in between, while the fourth provided the worst results compared with the other groups. For the same crystal, with the increase of muffin tin radius of lanthanum, the calculated energy band gaps decreased, going from semi conductor to semi metal. This again clearly indicated the sensitivity of energy band structure on muffin tin approximation.展开更多
The valences of Cu in Y_(1-x)Ca_(x)Ba_(2)Cu_(3)O_(6+y) have been investigated by using complex chemical bond theory.The results for the valences of Cu(1)and Cu(2)in the calculation suggest that the holes introduced by...The valences of Cu in Y_(1-x)Ca_(x)Ba_(2)Cu_(3)O_(6+y) have been investigated by using complex chemical bond theory.The results for the valences of Cu(1)and Cu(2)in the calculation suggest that the holes introduced by calcium substitution only reside in CuO_(2) planes,and there is a competition mechanism for the hole density in CuO_(2) planes between Ca doping and oxygen depletion.These conclusions are satisfactorily in agreement with experiments.展开更多
文摘目的分析比较盾叶薯蓣根茎与叶片的转录组,挖掘与盾叶薯蓣中皂苷元生物合成途径相关的关键酶基因。方法利用Illumina Hi Seq2000高通量测序技术对盾叶薯蓣根茎与叶片进行转录组测序,对测序结果进行注释分析;并结合根茎与叶片中皂苷元的含量测定结果,挖掘与盾叶薯蓣中皂苷元生物合成途径相关的关键酶基因;最后利用实时荧光定量PCR(q RT-PCR)技术对部分候选基因的表达模式进行分析。结果共获得了81 660个Unigene,有64.33%在NT、NR、Swiss-Prot、KOG、GO、KEGG数据库中得到注释;根据其表达量与代谢通路分析筛选出29种共227个可能参与盾叶薯蓣中皂苷元生物合成途径的催化酶基因,部分催化酶基因的表达模式与皂苷元含量具有一定相关性;并发现5个盾叶薯蓣内生菌基因。结论研究初步获得了盾叶薯蓣中参与皂苷元生物合成的候选关键酶基因,部分候选酶基因可能参与盾叶薯蓣中皂苷类成分的后修饰过程,并发现盾叶薯蓣根茎中内生菌可能参与其皂苷元的生物合成,为进一步阐明盾叶薯蓣中皂苷元生物合成的分子机制奠定基础。
基金Supported by the National Natural Science Foundation of China under Grant Nos 21173260,51072221 and 91233202the National Basic Research Program of China under Grant Nos 2012CB932903 and 2012CB932904the Knowledge Innovation Program of the Chinese Academy of Sciences.
文摘Metal-insulator-semiconductor back contact has been employed for a perovskite organic lead iodide heterojunction solar cell,in which an ultrathin Al_(2)O_(3) film as an insulating layer was deposited onto the CH_(3)NH_(3)PbI_(3) by atomic layer deposition technology.The light-to-electricity conversion efficiency of the devices is significantly enhanced from 3.30%to 5.07%.Further the impedance spectrum reveals that this insulating layer sustains part of the positive bias applied in the absorber region close to the back contact and decreases the carrier transport barrier,thus promoting transportation of carriers.
文摘The influence of muffin tin approximation on energy band gap was studied using LMTO ASA ( Linear Muffin Tin Orbital Atomic Sphere Approximation) approach. Since the diverse data are available for LaX(X=N, P, As, Sb), they are presented in our research as an example in order to test the reliability of our results. Four groups of muffin tin radii were chosen, they were the fitted muffin tin radii based on the optical properties of the crystals (the first), 1∶1 for La∶X(the second), 1 5∶1 for La∶X(the third), and a group of radii derived by making the charge in the interstitial space to be zero(the fourth). The results show that the fitted muffin tin radii (the first group) give the best results compared with experimental values, and the predicted energy band gaps are very sensitive to the choice of muffin tin radius in comparison with the other groups. The second and the third delivered results somewhere in between, while the fourth provided the worst results compared with the other groups. For the same crystal, with the increase of muffin tin radius of lanthanum, the calculated energy band gaps decreased, going from semi conductor to semi metal. This again clearly indicated the sensitivity of energy band structure on muffin tin approximation.
文摘The valences of Cu in Y_(1-x)Ca_(x)Ba_(2)Cu_(3)O_(6+y) have been investigated by using complex chemical bond theory.The results for the valences of Cu(1)and Cu(2)in the calculation suggest that the holes introduced by calcium substitution only reside in CuO_(2) planes,and there is a competition mechanism for the hole density in CuO_(2) planes between Ca doping and oxygen depletion.These conclusions are satisfactorily in agreement with experiments.
