Zinc(Zn)is an essential micronutrient for both plants and humans.Zn deficiency is common in many paddy fields and reduces yield and grain Zn content(GZC).To accelerate breeding for improved GZC and Zn deficiency toler...Zinc(Zn)is an essential micronutrient for both plants and humans.Zn deficiency is common in many paddy fields and reduces yield and grain Zn content(GZC).To accelerate breeding for improved GZC and Zn deficiency tolerance(ZDT)rice varieties,it is important to identify genes regulating Zn homeostasis.In this study,a member of the Ser/Thr protein phosphatase family,LOC_Os07g48840(named as OsGZ7),was found to contributed to ZDT and GZC in rice.The expression of OsGZ7 in roots and shoots was inhibited by Zn deficiency and toxicity,but induced by iron(Fe),manganese(Mn),and copper(Cu)deficiency,as well as chromium toxicity.OsGZ7 localized to the cytoplasm and was expressed in all tissues during the tillering,flowering,and grain-filling stages,particularly in the leaf blade and leaf sheath.At the seedling stage,knockout of OsGZ7 enhanced ZDT and increased Zn accumulation in both roots and shoots.At the maturity stage,knockout of OsGZ7 enhanced GZC,while overexpression of OsGZ7 reduced GZC.RNA-seq analysis suggested that OsGZ7 might regulate Zn homeostasis by affecting metal binding,hormone signal transduction,and oxidoreductase activity.Taken together,our findings indicate that OsGZ7 contributes to ZDT and Zn accumulation in rice.展开更多
针对现有的所有权转移协议,大多只涉及到单个标签的所有权转移过程,普遍存在隐私数据泄露、所有权转移过程不稳定等问题,该文在轻量级加密算法的基础上,提出一种改进的共享所有权转移协议(TSOTP,TTP model shared ownership transfer pr...针对现有的所有权转移协议,大多只涉及到单个标签的所有权转移过程,普遍存在隐私数据泄露、所有权转移过程不稳定等问题,该文在轻量级加密算法的基础上,提出一种改进的共享所有权转移协议(TSOTP,TTP model shared ownership transfer protocol),采用基于可信第三方(TTP,trusted third party)的对称加密机制,在完成初始标签认证后,通过TTP授权认证,使用对称加密算法,产生群组对称密钥,新所有者利用共享群组密钥对标签身份进行认证,然后为标签分配新的密钥,从而最终获得授权,读取标签中包含的药材敏感数据。TSOTP协议能够提高标签在所有权转移过程中的稳定性,很好地实现所有权在共享用户之间的安全转移,保证标签的数据安全,减少隐私数据泄露、Dos攻击、重放攻击等风险,提高前向与后向安全性,同时可以避免所有权重复转移,简化了标签认证计算量。经过试验证明,TSOTP协议与群组所有权转移协议(GOT,group ownership transfer)协议相比,标签数据库认证消耗时间节省57%,标签计算量消耗时间节省38%,能够成功阻止重放攻击和异步攻击等,具备较好的稳定性和认证效率,可以满足中药材质量溯源系统的研究需要,研究结果为建立中药材质量溯源系统的标签安全机制提供了技术参考。展开更多
基金supported by the National Key R&D Program of China(Grant No.2022YFE0139400)the National Natural Science Foundation of China(Grant No.31961143016)the Shenzhen Science and Technology Program,China(Grant No.JCYJ20200109150650397).
文摘Zinc(Zn)is an essential micronutrient for both plants and humans.Zn deficiency is common in many paddy fields and reduces yield and grain Zn content(GZC).To accelerate breeding for improved GZC and Zn deficiency tolerance(ZDT)rice varieties,it is important to identify genes regulating Zn homeostasis.In this study,a member of the Ser/Thr protein phosphatase family,LOC_Os07g48840(named as OsGZ7),was found to contributed to ZDT and GZC in rice.The expression of OsGZ7 in roots and shoots was inhibited by Zn deficiency and toxicity,but induced by iron(Fe),manganese(Mn),and copper(Cu)deficiency,as well as chromium toxicity.OsGZ7 localized to the cytoplasm and was expressed in all tissues during the tillering,flowering,and grain-filling stages,particularly in the leaf blade and leaf sheath.At the seedling stage,knockout of OsGZ7 enhanced ZDT and increased Zn accumulation in both roots and shoots.At the maturity stage,knockout of OsGZ7 enhanced GZC,while overexpression of OsGZ7 reduced GZC.RNA-seq analysis suggested that OsGZ7 might regulate Zn homeostasis by affecting metal binding,hormone signal transduction,and oxidoreductase activity.Taken together,our findings indicate that OsGZ7 contributes to ZDT and Zn accumulation in rice.
文摘针对现有的所有权转移协议,大多只涉及到单个标签的所有权转移过程,普遍存在隐私数据泄露、所有权转移过程不稳定等问题,该文在轻量级加密算法的基础上,提出一种改进的共享所有权转移协议(TSOTP,TTP model shared ownership transfer protocol),采用基于可信第三方(TTP,trusted third party)的对称加密机制,在完成初始标签认证后,通过TTP授权认证,使用对称加密算法,产生群组对称密钥,新所有者利用共享群组密钥对标签身份进行认证,然后为标签分配新的密钥,从而最终获得授权,读取标签中包含的药材敏感数据。TSOTP协议能够提高标签在所有权转移过程中的稳定性,很好地实现所有权在共享用户之间的安全转移,保证标签的数据安全,减少隐私数据泄露、Dos攻击、重放攻击等风险,提高前向与后向安全性,同时可以避免所有权重复转移,简化了标签认证计算量。经过试验证明,TSOTP协议与群组所有权转移协议(GOT,group ownership transfer)协议相比,标签数据库认证消耗时间节省57%,标签计算量消耗时间节省38%,能够成功阻止重放攻击和异步攻击等,具备较好的稳定性和认证效率,可以满足中药材质量溯源系统的研究需要,研究结果为建立中药材质量溯源系统的标签安全机制提供了技术参考。
