大豆蛋白加入小麦粉中改善面制品营养品质的同时也会对面团的面筋蛋白组成和结构产生影响。醇溶蛋白是小麦面筋的主要组分,赋予面团黏性和延伸性。为探究大豆蛋白对面团中醇溶蛋白的影响,将大豆分离蛋白(soy protein isolate,SPI)、大...大豆蛋白加入小麦粉中改善面制品营养品质的同时也会对面团的面筋蛋白组成和结构产生影响。醇溶蛋白是小麦面筋的主要组分,赋予面团黏性和延伸性。为探究大豆蛋白对面团中醇溶蛋白的影响,将大豆分离蛋白(soy protein isolate,SPI)、大豆浓缩蛋白(soy protein concentrate,SPC)、质构化大豆蛋白(texturized soy protein,TSP)分别以不同比例添加到小麦粉中制成面团,分离醇溶蛋白,通过内源荧光光谱、紫外光谱扫描及酸性聚丙烯酰胺电泳等方法对面团中醇溶蛋白的结构和组成进行评价。结果表明,与小麦粉面团相比,添加8%(质量分数,下同)的SPI、SPC、TSP后,面团中醇溶蛋白相对含量分别下降了9.65%、9.85%、5.97%;添加8%的SPI后,面团α-醇溶蛋白比例增加0.79%,β-,γ-醇溶蛋白比例无显著性变化,ω-醇溶蛋白比例下降2.27%;添加8%的SPC后,面团α-醇溶蛋白比例下降1.42%,β-醇溶蛋白比例下降1.59%,γ-醇溶蛋白无显著性变化,ω-醇溶蛋白比例增加3.25%;添加8%的TSP后,面团α-醇溶蛋白比例下降1.47%,β-,γ-醇溶蛋白比例无显著性变化,ω-醇溶蛋白比例增加1.12%。添加SPI和TSP均能使面团中醇溶蛋白肽链伸展,α-螺旋含量降低;添加SPI使面团中醇溶蛋白β-转角含量降低;添加SPC对面团中醇溶蛋白结构无显著影响。综上,添加大豆蛋白使面团中醇溶蛋白相对含量下降,肽链结构伸展,蛋白组分发生变化,从而改变醇溶蛋白黏附在麦谷蛋白骨架的状态,增大面团拉断力,缩短面团拉伸距离。该研究建立了一种探究面团中醇溶蛋白组成和结构的方法体系,为植物源蛋白在面制品中的应用提供理论基础和新思路。展开更多
为了探究醇法大豆浓缩蛋白(Aqueous-alcohol leached soybean protein concentrate,SPC)对面团及其蛋白特性的影响,本研究以大豆分离蛋白为参照,分析了SPC添加量(0%~8%)对面团面筋、拉伸和动态流变学特性及面团中蛋白质组分、二硫键含...为了探究醇法大豆浓缩蛋白(Aqueous-alcohol leached soybean protein concentrate,SPC)对面团及其蛋白特性的影响,本研究以大豆分离蛋白为参照,分析了SPC添加量(0%~8%)对面团面筋、拉伸和动态流变学特性及面团中蛋白质组分、二硫键含量和面团微观结构的影响。结果表明:随着SPC添加量的增加,面团湿面筋含量及拉伸阻力显著增加(P<0.05),面筋指数和拉伸距离显著下降(P<0.05)。添加SPC还提高了面团的储能模量(G′)和损耗模量(G"),当SPC添加量为8%时,面团具有最小的损耗因子(tanδ),此时面团弹性最突出。SPC的添加使面团中SDS可溶性蛋白相对含量显著增加(P<0.05),醇溶性蛋白和麦谷蛋白大聚体相对含量显著下降(P<0.05),此外,其还使面团中形成了新的二硫键。激光共聚焦分析结果证明了SPC的添加干扰了面筋网络的形成。综上所述,SPC通过二硫键与麦谷蛋白相互作用生成小的聚集体,使蛋白质组分相对含量及面筋网络结构发生变化,进一步导致面团特性发生了改变,且不同SPC添加量的面团特性存在明显差异。展开更多
The East Kunlun located in the northern margin of the Qinghai-Xizang(Tibet)Plateau,is a composite orogenic belt which has underwent multi-stages tectonic evolution(e.g.Wang and Chen,1987;Jiang et al.,1992;Yang et al.,...The East Kunlun located in the northern margin of the Qinghai-Xizang(Tibet)Plateau,is a composite orogenic belt which has underwent multi-stages tectonic evolution(e.g.Wang and Chen,1987;Jiang et al.,1992;Yang et al.,1996,2009).The East Kunlun orogenic belt(EKOB)is bounded by Altyn Tagh Fault in the west and Wenquan Fault in the east,bounded by the south margin of Qaidam展开更多
Coesite provides direct evidence for ultrahigh pressure metamorphism. Although coesite has been found as inclusions in zircon in paragneiss of the north Qaidam Mountains, it has never been identified in eclogite. In t...Coesite provides direct evidence for ultrahigh pressure metamorphism. Although coesite has been found as inclusions in zircon in paragneiss of the north Qaidam Mountains, it has never been identified in eclogite. In this contribution, based on petrographic observations and in situ Raman microprobe spectroscopy, coesite was identified as inclusions in garnet of eclogite from the Aercituoshan, Dulan UHP metamorphic unit, north Qaidam Mountains. Coesite is partly replaced by quartz, showing a pali-sade texture. This is the first report on coesite in eclogite from the north Qaidam Mountains, and is also supported by garnet-omphacite-phengite geothermobarometry (2.7―3.25 GPa, 670―730℃). Coesite and its pseudomorphs have not been found in eclogites and associated rocks of other units of the north Qaidam Mountains. Further studies are required to confirm if all metamorphic units in the north Qaidam Mountains underwent the ultrahigh-pressure metamorphism.展开更多
Zircon SHRIMP dating from Qaidamshan granite shows that the granite age is 446 Ma, similar to that of eclogite in the UHP belt. We think that both granite and eclogite may be formed at different stages during tectonic...Zircon SHRIMP dating from Qaidamshan granite shows that the granite age is 446 Ma, similar to that of eclogite in the UHP belt. We think that both granite and eclogite may be formed at different stages during tectonic evolution of this area. Together with other studies we suggested that the collision of ocean and continent plates may occur at the early Caledonian in this area, forming the eclogite, and the collision of continent and continent plates at the late Caledonian, forming the Qaidanishan granite with the zircon SHRIMP age of 446 Ma.展开更多
文摘大豆蛋白加入小麦粉中改善面制品营养品质的同时也会对面团的面筋蛋白组成和结构产生影响。醇溶蛋白是小麦面筋的主要组分,赋予面团黏性和延伸性。为探究大豆蛋白对面团中醇溶蛋白的影响,将大豆分离蛋白(soy protein isolate,SPI)、大豆浓缩蛋白(soy protein concentrate,SPC)、质构化大豆蛋白(texturized soy protein,TSP)分别以不同比例添加到小麦粉中制成面团,分离醇溶蛋白,通过内源荧光光谱、紫外光谱扫描及酸性聚丙烯酰胺电泳等方法对面团中醇溶蛋白的结构和组成进行评价。结果表明,与小麦粉面团相比,添加8%(质量分数,下同)的SPI、SPC、TSP后,面团中醇溶蛋白相对含量分别下降了9.65%、9.85%、5.97%;添加8%的SPI后,面团α-醇溶蛋白比例增加0.79%,β-,γ-醇溶蛋白比例无显著性变化,ω-醇溶蛋白比例下降2.27%;添加8%的SPC后,面团α-醇溶蛋白比例下降1.42%,β-醇溶蛋白比例下降1.59%,γ-醇溶蛋白无显著性变化,ω-醇溶蛋白比例增加3.25%;添加8%的TSP后,面团α-醇溶蛋白比例下降1.47%,β-,γ-醇溶蛋白比例无显著性变化,ω-醇溶蛋白比例增加1.12%。添加SPI和TSP均能使面团中醇溶蛋白肽链伸展,α-螺旋含量降低;添加SPI使面团中醇溶蛋白β-转角含量降低;添加SPC对面团中醇溶蛋白结构无显著影响。综上,添加大豆蛋白使面团中醇溶蛋白相对含量下降,肽链结构伸展,蛋白组分发生变化,从而改变醇溶蛋白黏附在麦谷蛋白骨架的状态,增大面团拉断力,缩短面团拉伸距离。该研究建立了一种探究面团中醇溶蛋白组成和结构的方法体系,为植物源蛋白在面制品中的应用提供理论基础和新思路。
文摘为了探究醇法大豆浓缩蛋白(Aqueous-alcohol leached soybean protein concentrate,SPC)对面团及其蛋白特性的影响,本研究以大豆分离蛋白为参照,分析了SPC添加量(0%~8%)对面团面筋、拉伸和动态流变学特性及面团中蛋白质组分、二硫键含量和面团微观结构的影响。结果表明:随着SPC添加量的增加,面团湿面筋含量及拉伸阻力显著增加(P<0.05),面筋指数和拉伸距离显著下降(P<0.05)。添加SPC还提高了面团的储能模量(G′)和损耗模量(G"),当SPC添加量为8%时,面团具有最小的损耗因子(tanδ),此时面团弹性最突出。SPC的添加使面团中SDS可溶性蛋白相对含量显著增加(P<0.05),醇溶性蛋白和麦谷蛋白大聚体相对含量显著下降(P<0.05),此外,其还使面团中形成了新的二硫键。激光共聚焦分析结果证明了SPC的添加干扰了面筋网络的形成。综上所述,SPC通过二硫键与麦谷蛋白相互作用生成小的聚集体,使蛋白质组分相对含量及面筋网络结构发生变化,进一步导致面团特性发生了改变,且不同SPC添加量的面团特性存在明显差异。
基金supported by National Natural Science Foundation of China(41072026,41272052)the China Geological Survey project(1212010918003,1212011120158)
文摘The East Kunlun located in the northern margin of the Qinghai-Xizang(Tibet)Plateau,is a composite orogenic belt which has underwent multi-stages tectonic evolution(e.g.Wang and Chen,1987;Jiang et al.,1992;Yang et al.,1996,2009).The East Kunlun orogenic belt(EKOB)is bounded by Altyn Tagh Fault in the west and Wenquan Fault in the east,bounded by the south margin of Qaidam
基金Supported by National Natural Science Foundation of China (Grant Nos.41772138, 40472102 and 40272095)Program of Excellent Young Scientists of the Ministry of Land and Resources of ChinaGeological Survey Project of China Geological Survey (Grant No. 1212010611811)
文摘Coesite provides direct evidence for ultrahigh pressure metamorphism. Although coesite has been found as inclusions in zircon in paragneiss of the north Qaidam Mountains, it has never been identified in eclogite. In this contribution, based on petrographic observations and in situ Raman microprobe spectroscopy, coesite was identified as inclusions in garnet of eclogite from the Aercituoshan, Dulan UHP metamorphic unit, north Qaidam Mountains. Coesite is partly replaced by quartz, showing a pali-sade texture. This is the first report on coesite in eclogite from the north Qaidam Mountains, and is also supported by garnet-omphacite-phengite geothermobarometry (2.7―3.25 GPa, 670―730℃). Coesite and its pseudomorphs have not been found in eclogites and associated rocks of other units of the north Qaidam Mountains. Further studies are required to confirm if all metamorphic units in the north Qaidam Mountains underwent the ultrahigh-pressure metamorphism.
文摘Zircon SHRIMP dating from Qaidamshan granite shows that the granite age is 446 Ma, similar to that of eclogite in the UHP belt. We think that both granite and eclogite may be formed at different stages during tectonic evolution of this area. Together with other studies we suggested that the collision of ocean and continent plates may occur at the early Caledonian in this area, forming the eclogite, and the collision of continent and continent plates at the late Caledonian, forming the Qaidanishan granite with the zircon SHRIMP age of 446 Ma.
