[Objectives]To distinguish loquat flesh color,thereby guiding early-age selection in loquat hybrid progeny.[Methods]Molecular markers based on the phytoene synthase gene(PSY)of loquat were applied to a hybrid progeny ...[Objectives]To distinguish loquat flesh color,thereby guiding early-age selection in loquat hybrid progeny.[Methods]Molecular markers based on the phytoene synthase gene(PSY)of loquat were applied to a hybrid progeny population derived from a cross between‘Zaozhong 6’and a local wild white-fleshed loquat resource‘DB1’.[Results]Among the 49 hybrid progeny,24 were identified as white-fleshed loquat resources and 25 as yellow-fleshed loquat resources.[Conclusions]The molecular marker of the PSY gene can effectively distinguish loquat flesh color and is of significant importance for guiding early-age selection in loquat hybrid breeding.展开更多
Small extracellular vesicles(sEVs)membrane protein profile(sEVpp)is a novel biomarker for cancer,and it can reveal the in-depth phenotype information.The point-of-care testing(POCT)of sEVpp holds great significance fo...Small extracellular vesicles(sEVs)membrane protein profile(sEVpp)is a novel biomarker for cancer,and it can reveal the in-depth phenotype information.The point-of-care testing(POCT)of sEVpp holds great significance for mass screening of cancer,so the cost-effective and simple detection methods of sEVpp are urgently demanded.Herein,we constructed a paper-based multichannel sEVpp POCT device(sEVpp-PAD)enabled by functional DNA probes and metal-organic framework(MOF).The core components are aptamer/MOF-modified paper chips.The modified aptamers can immunocapture the sEV expressing corresponding proteins,while the modified MOF can provide abundant sites for aptamer-modification,reduce the nonspecific protein absorption,and act as reference for ratiometric detection.Simply powered by two syringes,the sEVpp-PAD can efficiently capture sEVs expressing corresponding protein from cell culture media and sera.Furthermore,a detection probe(DP)consisted of CD63 aptamer and G-quadruplex was developed for the colorimetric detection of captured sEVs.Utilizing this device,the sEVpp in various hepatocellular carcinoma cell culture medium and,more importantly,in human sera can be accurately determined,only with$2 device,$0.2 detection reagents and 1.8 h procedure.This simple strategy for sEVpp detection can innovatively promote the POCT and subtyping of cancer based on sEV-related liquid biopsy.展开更多
基金Supported by Chengdu Science and Technology ProjectResearch and Development of Key Production Technologies and Scientific and Technological Services for Characteristic Fruit Trees in Lezhi County,Ziyang City(Science and Technology Commissioner)(2025-YF05-00549-SN).
文摘[Objectives]To distinguish loquat flesh color,thereby guiding early-age selection in loquat hybrid progeny.[Methods]Molecular markers based on the phytoene synthase gene(PSY)of loquat were applied to a hybrid progeny population derived from a cross between‘Zaozhong 6’and a local wild white-fleshed loquat resource‘DB1’.[Results]Among the 49 hybrid progeny,24 were identified as white-fleshed loquat resources and 25 as yellow-fleshed loquat resources.[Conclusions]The molecular marker of the PSY gene can effectively distinguish loquat flesh color and is of significant importance for guiding early-age selection in loquat hybrid breeding.
基金supported by the National Key Research and Development Program of China(No.2022YFE0201800)the National Natural Science Foundation of China(Nos.22274169 and 22474161)+5 种基金Guangdong Basic and Applied Basic Research Foundation(No.2024A1515030160)the Science and Technology Program of Guangzhou City(No.2023B03J1380)Shenzhen Science and Technology Innovation Commission(No.GJHZ20210705142200001)the Scientific Technology Project of Guangzhou City(No.202103000003)the Guangdong Science and Technology Plan Project(No.2020B1212060077)the Open Research Fund of State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University.
文摘Small extracellular vesicles(sEVs)membrane protein profile(sEVpp)is a novel biomarker for cancer,and it can reveal the in-depth phenotype information.The point-of-care testing(POCT)of sEVpp holds great significance for mass screening of cancer,so the cost-effective and simple detection methods of sEVpp are urgently demanded.Herein,we constructed a paper-based multichannel sEVpp POCT device(sEVpp-PAD)enabled by functional DNA probes and metal-organic framework(MOF).The core components are aptamer/MOF-modified paper chips.The modified aptamers can immunocapture the sEV expressing corresponding proteins,while the modified MOF can provide abundant sites for aptamer-modification,reduce the nonspecific protein absorption,and act as reference for ratiometric detection.Simply powered by two syringes,the sEVpp-PAD can efficiently capture sEVs expressing corresponding protein from cell culture media and sera.Furthermore,a detection probe(DP)consisted of CD63 aptamer and G-quadruplex was developed for the colorimetric detection of captured sEVs.Utilizing this device,the sEVpp in various hepatocellular carcinoma cell culture medium and,more importantly,in human sera can be accurately determined,only with$2 device,$0.2 detection reagents and 1.8 h procedure.This simple strategy for sEVpp detection can innovatively promote the POCT and subtyping of cancer based on sEV-related liquid biopsy.
