目的比较老年营养风险指数(GNRI)与营养风险筛查2002(NRS2002)在糖尿病肾病(DKD)患者中的应用价值。方法选取DKD患者218例,采用GNRI及NRS2002分别对其进行营养风险筛查。收集患者相关营养及肾功能指标,对两种筛查工具评分与上述生化指...目的比较老年营养风险指数(GNRI)与营养风险筛查2002(NRS2002)在糖尿病肾病(DKD)患者中的应用价值。方法选取DKD患者218例,采用GNRI及NRS2002分别对其进行营养风险筛查。收集患者相关营养及肾功能指标,对两种筛查工具评分与上述生化指标进行相关性分析。结果218例患者中GNRI评分<92分的有91例(41.7%),NRS2002评分≥3分的有51例(23.4%),二者营养风险筛查结果的一致性不理想。两种筛查工具得分与营养指标体质指数(BMI)、血清白蛋白(Alb)、血清前白蛋白(PA)、血红蛋白(HGB)均具有相关性(P<0.01)。GNRI得分与肾功能指标(尿微量白蛋白/尿肌酐)ACR、肾小球滤过虑(eGFR)、随机尿蛋白/随机尿肌酐(Up/Ucr)、24 h尿蛋白(24 h UP)、肌酐(Cr)、尿素(BUN)具有相关性(P<0.01),NRS2002得分与肾功能指标无相关性(P>0.05)。结论相比NRS2002,GNRI能更好地反映DKD患者营养及肾功能状况,适宜在临床推广应用,为DKD患者的精准营养支持提供科学依据。展开更多
Sesame （Sesamue indicum L.） is one of the most important oilseed crops with high oil yield. Here, we described a simple and efficient method for constructing a normalized cDNA library from a high oil content cultiva...Sesame （Sesamue indicum L.） is one of the most important oilseed crops with high oil yield. Here, we described a simple and efficient method for constructing a normalized cDNA library from a high oil content cultivar of sesame Zhongzhi 14, during its oil accumulation stages. It combined switching mechanism at 5？end of RNA transcript （SMART） technique and duplex-specific nuclease （DSN） normalization methods. Double-stranded cDNAs were synthesized from mRNAs, processed by normalization and Sfi I restriction endonuclease, and finally the cDNAs were ligated to pDNR-LIB vector. The ligation mixture was transformed into Escherichia coli DH10B by electroporation. The capacity of the library was 1.0？06 clones in this library. Gel electrophoresis results indicated the fragments ranged from 700 to 2 000 bp, with the average size of 1 800 bp. Random picking clones showed that the recombination rate was 100%. The results showed that the cDNA library constructed successfully was a full-length library with high quality, and could be used to screen the genes related to development of oil synthesis.展开更多
氧化石墨烯(Graphene oxide,GO)表面具有丰富的官能团和较高的比表面积,能够作为膜材料应用于膜分离技术.然而,目前合成GO的技术很难保证其横向尺寸的均一性.此外,GO在自然水环境中分散性的稳定性受环境中pH值和离子强度的影响.本文研...氧化石墨烯(Graphene oxide,GO)表面具有丰富的官能团和较高的比表面积,能够作为膜材料应用于膜分离技术.然而,目前合成GO的技术很难保证其横向尺寸的均一性.此外,GO在自然水环境中分散性的稳定性受环境中pH值和离子强度的影响.本文研究了横向尺寸、pH值和离子强度对GO表面双电层电荷或结构组装所需的相互作用力的影响.从原位原子力显微镜(Atomic force microscope,AFM)获得的力-距离曲线(F-D)可以看出,溶液条件对DLVO力的作用.GO的双电层静电斥力随着pH值的升高而增大,这可能是由于表面官能团的电离作用增强所致.但随着离子强度的增加,双电层斥力减小,得到的数据与DLVO理论一致.通过Zeta电位和开尔文探针力显微镜(Kelvin probe force microscopy,KPFM)测量,确定了氧化石墨烯片层表面电荷的不均匀性.展开更多
文摘目的比较老年营养风险指数(GNRI)与营养风险筛查2002(NRS2002)在糖尿病肾病(DKD)患者中的应用价值。方法选取DKD患者218例,采用GNRI及NRS2002分别对其进行营养风险筛查。收集患者相关营养及肾功能指标,对两种筛查工具评分与上述生化指标进行相关性分析。结果218例患者中GNRI评分<92分的有91例(41.7%),NRS2002评分≥3分的有51例(23.4%),二者营养风险筛查结果的一致性不理想。两种筛查工具得分与营养指标体质指数(BMI)、血清白蛋白(Alb)、血清前白蛋白(PA)、血红蛋白(HGB)均具有相关性(P<0.01)。GNRI得分与肾功能指标(尿微量白蛋白/尿肌酐)ACR、肾小球滤过虑(eGFR)、随机尿蛋白/随机尿肌酐(Up/Ucr)、24 h尿蛋白(24 h UP)、肌酐(Cr)、尿素(BUN)具有相关性(P<0.01),NRS2002得分与肾功能指标无相关性(P>0.05)。结论相比NRS2002,GNRI能更好地反映DKD患者营养及肾功能状况,适宜在临床推广应用,为DKD患者的精准营养支持提供科学依据。
基金supported by the National Basic Research Program of China (2011cb109305)the Genetically Modified Organisms Breeding Major Projects, China (2009zx08004-002B)+1 种基金the Open Project Program of Key Laboratory for Oil Crops Biology, the Ministry of Agriculture, China (200703)the Foundation of Oil Crops Research Institute, Chinese Academy of Agricultural Sciences
文摘Sesame （Sesamue indicum L.） is one of the most important oilseed crops with high oil yield. Here, we described a simple and efficient method for constructing a normalized cDNA library from a high oil content cultivar of sesame Zhongzhi 14, during its oil accumulation stages. It combined switching mechanism at 5？end of RNA transcript （SMART） technique and duplex-specific nuclease （DSN） normalization methods. Double-stranded cDNAs were synthesized from mRNAs, processed by normalization and Sfi I restriction endonuclease, and finally the cDNAs were ligated to pDNR-LIB vector. The ligation mixture was transformed into Escherichia coli DH10B by electroporation. The capacity of the library was 1.0？06 clones in this library. Gel electrophoresis results indicated the fragments ranged from 700 to 2 000 bp, with the average size of 1 800 bp. Random picking clones showed that the recombination rate was 100%. The results showed that the cDNA library constructed successfully was a full-length library with high quality, and could be used to screen the genes related to development of oil synthesis.
文摘氧化石墨烯(Graphene oxide,GO)表面具有丰富的官能团和较高的比表面积,能够作为膜材料应用于膜分离技术.然而,目前合成GO的技术很难保证其横向尺寸的均一性.此外,GO在自然水环境中分散性的稳定性受环境中pH值和离子强度的影响.本文研究了横向尺寸、pH值和离子强度对GO表面双电层电荷或结构组装所需的相互作用力的影响.从原位原子力显微镜(Atomic force microscope,AFM)获得的力-距离曲线(F-D)可以看出,溶液条件对DLVO力的作用.GO的双电层静电斥力随着pH值的升高而增大,这可能是由于表面官能团的电离作用增强所致.但随着离子强度的增加,双电层斥力减小,得到的数据与DLVO理论一致.通过Zeta电位和开尔文探针力显微镜(Kelvin probe force microscopy,KPFM)测量,确定了氧化石墨烯片层表面电荷的不均匀性.
