目的检测肠易激综合征大鼠肠道菌群及体内氨基酸的变化,探索应激致肠易激综合征的发生机制。方法采用母婴分离应激的方法建立肠易激综合征动物模型,收集大鼠的粪便和血清。采用高通量测序技术对粪便样本中所有细菌16S r DNA的V3、V4和V...目的检测肠易激综合征大鼠肠道菌群及体内氨基酸的变化,探索应激致肠易激综合征的发生机制。方法采用母婴分离应激的方法建立肠易激综合征动物模型,收集大鼠的粪便和血清。采用高通量测序技术对粪便样本中所有细菌16S r DNA的V3、V4和V6区进行测序,并进行菌群的生物信息学分析。采用全自动氨基酸分析仪检测分析大鼠粪便和血清样本的20种常见氨基酸含量。结果与正常大鼠相比,肠易激综合征大鼠肠道菌群的Shannon指数下降,表明菌群多样性有所下降。肠易激综合征大鼠的毛螺菌科、克里斯滕森菌科、单形拟杆菌、δ变形菌纲以及脱硫弧菌目、脱硫弧菌科和脱硫弧菌属的相对丰度均显著升高。同时,肠易激综合征大鼠粪便中的苏氨酸、丝氨酸、蛋氨酸、赖氨酸和脯氨酸浓度显著增高,血清中蛋氨酸、脯氨酸和苯丙氨酸浓度显著升高,甘氨酸和赖氨酸浓度则显著下降。结论应激引起肠道菌群失调以及氨基酸代谢异常,该变化可能与慢性应激导致的肠易激综合征有关。展开更多
The differential evolution (DE) algorithm is applied to solving themodels''equations of a whole missile power system, and the steady fault characteristics of the wholesystem are analyzed. The DE algorithm is r...The differential evolution (DE) algorithm is applied to solving themodels''equations of a whole missile power system, and the steady fault characteristics of the wholesystem are analyzed. The DE algorithm is robust, requires few control variables, is easy to use andlends itself very well to parallel computation. Calculation results indicate that the DE algorithmsimulates faults of a missile power system very well.展开更多
Primary and secondary school teachers teach primary and secondary school students to learn moral and legal knowledge, improve their values and awareness of laws and regulations, improve their moral and legal quality, ...Primary and secondary school teachers teach primary and secondary school students to learn moral and legal knowledge, improve their values and awareness of laws and regulations, improve their moral and legal quality, rely on emotional security to teach, to convince people with reason, to move with emotion, the effect is more significant. Based on the actual learning situation of primary and secondary school students, this paper actively analyzes the infiltration effect of emotional education factors in primary and secondary school moral and legal classroom teaching, and finds that there are certain problems in the current primary and secondary school moral and legal education standards. If primary and secondary school teachers teaching techniques are not professional and their teaching strategies are monotonous and obsolete, a series of negative effects will be produced. Only by properly handling the issues of rules and regulations and emotional consciousness can the classroom teaching of morality and law in primary and secondary schools play an active role.展开更多
文摘目的检测肠易激综合征大鼠肠道菌群及体内氨基酸的变化,探索应激致肠易激综合征的发生机制。方法采用母婴分离应激的方法建立肠易激综合征动物模型,收集大鼠的粪便和血清。采用高通量测序技术对粪便样本中所有细菌16S r DNA的V3、V4和V6区进行测序,并进行菌群的生物信息学分析。采用全自动氨基酸分析仪检测分析大鼠粪便和血清样本的20种常见氨基酸含量。结果与正常大鼠相比,肠易激综合征大鼠肠道菌群的Shannon指数下降,表明菌群多样性有所下降。肠易激综合征大鼠的毛螺菌科、克里斯滕森菌科、单形拟杆菌、δ变形菌纲以及脱硫弧菌目、脱硫弧菌科和脱硫弧菌属的相对丰度均显著升高。同时,肠易激综合征大鼠粪便中的苏氨酸、丝氨酸、蛋氨酸、赖氨酸和脯氨酸浓度显著增高,血清中蛋氨酸、脯氨酸和苯丙氨酸浓度显著升高,甘氨酸和赖氨酸浓度则显著下降。结论应激引起肠道菌群失调以及氨基酸代谢异常,该变化可能与慢性应激导致的肠易激综合征有关。
文摘The differential evolution (DE) algorithm is applied to solving themodels''equations of a whole missile power system, and the steady fault characteristics of the wholesystem are analyzed. The DE algorithm is robust, requires few control variables, is easy to use andlends itself very well to parallel computation. Calculation results indicate that the DE algorithmsimulates faults of a missile power system very well.
文摘Primary and secondary school teachers teach primary and secondary school students to learn moral and legal knowledge, improve their values and awareness of laws and regulations, improve their moral and legal quality, rely on emotional security to teach, to convince people with reason, to move with emotion, the effect is more significant. Based on the actual learning situation of primary and secondary school students, this paper actively analyzes the infiltration effect of emotional education factors in primary and secondary school moral and legal classroom teaching, and finds that there are certain problems in the current primary and secondary school moral and legal education standards. If primary and secondary school teachers teaching techniques are not professional and their teaching strategies are monotonous and obsolete, a series of negative effects will be produced. Only by properly handling the issues of rules and regulations and emotional consciousness can the classroom teaching of morality and law in primary and secondary schools play an active role.
