目的本研究拟证明卵巢癌细胞基因修复功能损伤的程度和程序性死亡配体-1(Programmed death ligand-1,PD-L1)之间的关系,为PD-L1治疗卵巢癌提供依据。方法采用卵巢癌组织芯片(tissue microarray,TMA)样本,非小细胞肺癌TMA为对照。采用辅...目的本研究拟证明卵巢癌细胞基因修复功能损伤的程度和程序性死亡配体-1(Programmed death ligand-1,PD-L1)之间的关系,为PD-L1治疗卵巢癌提供依据。方法采用卵巢癌组织芯片(tissue microarray,TMA)样本,非小细胞肺癌TMA为对照。采用辅助损伤修复检测(repair assisted damage detection,RADD)法和改良的RADD法检测DNA损伤及修复功能缺陷水平。应用免疫组化方法观察卵巢肿瘤细胞中DNA损伤和PD-L1蛋白表达,采用激光共聚焦显微镜对TMAs内的每个组织核芯进行成像并行图像采集。记录每个核芯的荧光强度,绘制图像并计算二者的相关性。结果RADD法和改良RADD法都能有效的检测出肿瘤组织中的DNA损伤水平,其中改良的RADD(mRADD)法检出水平更高。卵巢肿瘤中DNA氧化损伤的发生率很高。随着DNA氧化损伤水平增加,PD-L1在肿瘤中的表达也随之增加。与正常组织相比,高级别浆液性腺癌和子宫内膜样腺癌的DNA损伤水平差异最明显。而且PD-L1表达和DNA损伤水平有很好的相关性。相关性分析发现,采用改良RADD法检测高级别浆液性癌和子宫内膜样腺癌的DNA损伤水平与PD-L1之间都具有良好的相关性。卵巢粘液性腺癌中的DNA损伤水平与PD-L1表达的相关性最高。结论卵巢癌DNA氧化损伤或其与PD-L1协同表达关系可能是改善PD-L1阻断治疗有效性分类筛选中的一个潜在生物标志物。展开更多
The proper establishment of loop-vessel countercurrent arrangement in medulla provides the basis for formation of the concentrated urine.To date,the morphogenesis of it has not been fully discovered.The present study ...The proper establishment of loop-vessel countercurrent arrangement in medulla provides the basis for formation of the concentrated urine.To date,the morphogenesis of it has not been fully discovered.The present study investigated the spatial arrangement of nephron loop and vessel of developing kidneys using 3D visualization technique and immunohistochemistry for AQP-1 and NKCC2.Five-mm-thick serial paraffin sections were obtained from E14.5 and P5 kidneys and stained with hematoxylin,while 2.5-mm-thick serial epoxy sections were obtained from E17.5 and P56 kidneys and stained with toluidine blue.展开更多
The structure and properties of Sm overlayer and Sm/Rh surface alloy have been investigated with Auger electron spectroscopy (AES), low energy electron diffraction (LEED), X-ray photoelectron spectroscopy (XPS), and t...The structure and properties of Sm overlayer and Sm/Rh surface alloy have been investigated with Auger electron spectroscopy (AES), low energy electron diffraction (LEED), X-ray photoelectron spectroscopy (XPS), and temperature programmed desorption spectroscopy (TDS). The growth of Sm on Rh(100) at room temperature (RT) appears following the Stranski-Krastanov growth mode and only the trivalent state Sm is observed from XPS results. Thermal treatment of the Sm film at 900 K leads to the formation of ordered surface alloy which shows the c R45°and c(2 ×2) LEED patterns. Annealing the Sm film at temperature above 400 K makes the binding energy (B.E.) of Sm 3d5/2 shift to higher energy by 0.7 eV, which indicates charge transfer from Sm to Rh(100) substrate, causing the increase of CO desorption temperature.展开更多
We report our investigation of the interaction of NO2 with the Au(997)vicinal surface by high-resolution photoelectron spectroscopy using synchrotron radiation as the excitation source.At 170 K,both core-level and val...We report our investigation of the interaction of NO2 with the Au(997)vicinal surface by high-resolution photoelectron spectroscopy using synchrotron radiation as the excitation source.At 170 K,both core-level and valence-band photoemission results illustrate the decomposition of NO2 on the Au(997)surface at low NO2 exposures,forming coadsorbed NO(a)and O(a)species.After annealing at 300 K,NO(a)desorbs from Au(997)whereas O(a)remains on the surface.Upon annealing at 750 K,we observe no signal for adsorbed oxygen on Au(997).These results clearly demonstrate that thermal decomposition of NO2 is an effective method to generate oxygen adatoms on Au(997)under ultrahigh-vacuum conditions.展开更多
Background The prevalence of metabolic syndrome （MetS） increased recently and there was still not a screening index to predict MetS.The aim of this study was to estimate whether brachial-ankle pulse wave velocity （...Background The prevalence of metabolic syndrome （MetS） increased recently and there was still not a screening index to predict MetS.The aim of this study was to estimate whether brachial-ankle pulse wave velocity （baPVVV）,a novel marker for systemic arterial stiffness,could predict MetS in Chinese community population.Methods A total of 2 191 participants were recruited and underwent medical examination including 1 455 men and 756 women from June 2011 to January 2012.MetS was diagnosed according to the criteria of the International Diabetes Federation （IDF）.Multiple Logistic regressions were conducted to explore the risk factors of MetS.Receiver operating characteristic （ROC） curve was performed to estimate the ideal diagnostic cutoff point of baPWV to predict MetS.Results The mean age was （45.35±8.27） years old.In multiple Logistic regression analysis,the gender,baPWV and smoking status were risk factors to MetS after adjusting age,gender,baPWV,walk time and sleeping time.The prevalence of MetS was 17.48% in 30-year age population in Shanghai.There were significant differences （Х^2=96.46,P 〈0.05） between male and female participants on MetS prevalence.According to the ROC analyses,the ideal cutoff point of baPWV was 1 358.50 cm/s （AUC=60.20%） to predict MetS among male group and 1 350.00 cm/s （AUC=70.90%） among female group.Conclusion BaPWV may be considered as a screening marker to predict MetS in community Chinese population and the diagnostic value of 1 350.00 cm/s was more significant for the female group.展开更多
The preparation of SmOx/Rh(100) and CO adsorption on this model surface have been investigated with Auger electron spectroscopy (AES), X-ray photoelectron spectroscopy (XPS) and temperature programmed desorption spect...The preparation of SmOx/Rh(100) and CO adsorption on this model surface have been investigated with Auger electron spectroscopy (AES), X-ray photoelectron spectroscopy (XPS) and temperature programmed desorption spectroscopy (TDS). The oxygen adsorption on the SmRh alloy surface leads to the aggregation of Sm on the surface. The thermal treatment of this oxidized surface induces the further agglomeration of SmOx on the Rh(100) surface. Compared with CO TDS on the clean Rh(100) surface, three additional CO desorption peaks can be observed at 176,331 and 600 K on the SmOx/Rh(100) surface. The CO desorption peak at 176 K may originate from CO adsorbed on SmOx islands, while the appearance of the CO adsorption peaks at 331 and 600 K, depending on the oxidation state of Sm, is attributed to CO species located at the interface of SmOx/Rh(100).展开更多
We report a detailed investigation of the behavior of chemisorbed hydrogen atoms (Ha) on Pt(111) by a combination of an ex-perimental study of the Ha + Da reaction and first-principles calculations. The coverage-depen...We report a detailed investigation of the behavior of chemisorbed hydrogen atoms (Ha) on Pt(111) by a combination of an ex-perimental study of the Ha + Da reaction and first-principles calculations. The coverage-dependent adsorption and desorption behavior of Ha and Da on Pt(111) have been systematically established and can be well interpreted in terms of repulsive inter-actions between adsorbates. Ha adsorbs exclusively on the face-centered cubic (fcc) sites of Pt(111) at coverages not exceeding 1 monolayer (ML). With increasing Ha coverage,repulsive interactions between Ha increase,leading to a reduction in both the adsorption energy and the desorption activation energy. It is proposed that the lateral interactions within a Ha layer are partly induced by the local repulsive interactions due to high mobility of Ha on Pt(111). For the Ha + Da exchange reaction on Pt(111),it is found that Ha has a higher selectivity for HD formation than Da. Considering that Ha diffuses much faster than Da on Pt(111),we propose that the difference in diffusion rates between Ha and Da may determine the selectivity of Ha and Da in forming HD in the Ha + Da reaction on Pt(111).展开更多
文摘The proper establishment of loop-vessel countercurrent arrangement in medulla provides the basis for formation of the concentrated urine.To date,the morphogenesis of it has not been fully discovered.The present study investigated the spatial arrangement of nephron loop and vessel of developing kidneys using 3D visualization technique and immunohistochemistry for AQP-1 and NKCC2.Five-mm-thick serial paraffin sections were obtained from E14.5 and P5 kidneys and stained with hematoxylin,while 2.5-mm-thick serial epoxy sections were obtained from E17.5 and P56 kidneys and stained with toluidine blue.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 29873042) and the State Key Laboratory of Catalysis, the Chinese Academy of Sciences.
文摘The structure and properties of Sm overlayer and Sm/Rh surface alloy have been investigated with Auger electron spectroscopy (AES), low energy electron diffraction (LEED), X-ray photoelectron spectroscopy (XPS), and temperature programmed desorption spectroscopy (TDS). The growth of Sm on Rh(100) at room temperature (RT) appears following the Stranski-Krastanov growth mode and only the trivalent state Sm is observed from XPS results. Thermal treatment of the Sm film at 900 K leads to the formation of ordered surface alloy which shows the c R45°and c(2 ×2) LEED patterns. Annealing the Sm film at temperature above 400 K makes the binding energy (B.E.) of Sm 3d5/2 shift to higher energy by 0.7 eV, which indicates charge transfer from Sm to Rh(100) substrate, causing the increase of CO desorption temperature.
基金supported by the National Natural Science Foundation of China(20803072,20973161)National Basic Research Program of China(2010CB923302)the MPG-CAS partner group program
文摘We report our investigation of the interaction of NO2 with the Au(997)vicinal surface by high-resolution photoelectron spectroscopy using synchrotron radiation as the excitation source.At 170 K,both core-level and valence-band photoemission results illustrate the decomposition of NO2 on the Au(997)surface at low NO2 exposures,forming coadsorbed NO(a)and O(a)species.After annealing at 300 K,NO(a)desorbs from Au(997)whereas O(a)remains on the surface.Upon annealing at 750 K,we observe no signal for adsorbed oxygen on Au(997).These results clearly demonstrate that thermal decomposition of NO2 is an effective method to generate oxygen adatoms on Au(997)under ultrahigh-vacuum conditions.
基金This work was supported by grants from the National Natural Science Foundation of China （No. 81170325）, International S＆T Cooperation Program of China （No. 2011DFB30010）.Acknowledgements： We are grateful to all subjects for their enthusiastic participation. We are also indebted to Xiu Jianfeng and Wu Lezou for their pioneering work.
文摘Background The prevalence of metabolic syndrome （MetS） increased recently and there was still not a screening index to predict MetS.The aim of this study was to estimate whether brachial-ankle pulse wave velocity （baPVVV）,a novel marker for systemic arterial stiffness,could predict MetS in Chinese community population.Methods A total of 2 191 participants were recruited and underwent medical examination including 1 455 men and 756 women from June 2011 to January 2012.MetS was diagnosed according to the criteria of the International Diabetes Federation （IDF）.Multiple Logistic regressions were conducted to explore the risk factors of MetS.Receiver operating characteristic （ROC） curve was performed to estimate the ideal diagnostic cutoff point of baPWV to predict MetS.Results The mean age was （45.35±8.27） years old.In multiple Logistic regression analysis,the gender,baPWV and smoking status were risk factors to MetS after adjusting age,gender,baPWV,walk time and sleeping time.The prevalence of MetS was 17.48% in 30-year age population in Shanghai.There were significant differences （Х^2=96.46,P 〈0.05） between male and female participants on MetS prevalence.According to the ROC analyses,the ideal cutoff point of baPWV was 1 358.50 cm/s （AUC=60.20%） to predict MetS among male group and 1 350.00 cm/s （AUC=70.90%） among female group.Conclusion BaPWV may be considered as a screening marker to predict MetS in community Chinese population and the diagnostic value of 1 350.00 cm/s was more significant for the female group.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 29873042)the State Key Laboratory of Catalysis+1 种基金 Dalian Institute of Chemical Physicsthe Chinese Academy of Sciences.
文摘The preparation of SmOx/Rh(100) and CO adsorption on this model surface have been investigated with Auger electron spectroscopy (AES), X-ray photoelectron spectroscopy (XPS) and temperature programmed desorption spectroscopy (TDS). The oxygen adsorption on the SmRh alloy surface leads to the aggregation of Sm on the surface. The thermal treatment of this oxidized surface induces the further agglomeration of SmOx on the Rh(100) surface. Compared with CO TDS on the clean Rh(100) surface, three additional CO desorption peaks can be observed at 176,331 and 600 K on the SmOx/Rh(100) surface. The CO desorption peak at 176 K may originate from CO adsorbed on SmOx islands, while the appearance of the CO adsorption peaks at 331 and 600 K, depending on the oxidation state of Sm, is attributed to CO species located at the interface of SmOx/Rh(100).
基金supported by the National Natural Science Foundation of China (NSFC20503027, NSFC20773113, NSFC20803072)the Ministry of Sci-ence and Technology of China, MOE Program for PCSIRT (IRT0756)
文摘We report a detailed investigation of the behavior of chemisorbed hydrogen atoms (Ha) on Pt(111) by a combination of an ex-perimental study of the Ha + Da reaction and first-principles calculations. The coverage-dependent adsorption and desorption behavior of Ha and Da on Pt(111) have been systematically established and can be well interpreted in terms of repulsive inter-actions between adsorbates. Ha adsorbs exclusively on the face-centered cubic (fcc) sites of Pt(111) at coverages not exceeding 1 monolayer (ML). With increasing Ha coverage,repulsive interactions between Ha increase,leading to a reduction in both the adsorption energy and the desorption activation energy. It is proposed that the lateral interactions within a Ha layer are partly induced by the local repulsive interactions due to high mobility of Ha on Pt(111). For the Ha + Da exchange reaction on Pt(111),it is found that Ha has a higher selectivity for HD formation than Da. Considering that Ha diffuses much faster than Da on Pt(111),we propose that the difference in diffusion rates between Ha and Da may determine the selectivity of Ha and Da in forming HD in the Ha + Da reaction on Pt(111).
