OBJECTIVE:To investigate the efficacy of Astragaloside IV(AS-IV)on radiation-induced liver inflammation in mice.METHODS:The mice were divided into normal group,dimethyl sulfoxide solvent group,irradiation group(IR),ir...OBJECTIVE:To investigate the efficacy of Astragaloside IV(AS-IV)on radiation-induced liver inflammation in mice.METHODS:The mice were divided into normal group,dimethyl sulfoxide solvent group,irradiation group(IR),irradiation+AS-IV(20 mg/kg)group(IR+AS-20)and irradiation+AS-IV(40 mg/kg)group(IR+AS-40).One month after intraperitoneal injection of AS-IV,the mice were irradiated with 8Gry Co60γ,the blood was collected for biochemical analysis,and the liver was collected for hematoxylin-eosin staining,immunofluorescence and electron microscopic observation,oxidative stress,and Western blot analysis.RESULTS:The AS-IV treatment significantly ameliorated the pathological morphology of liver and reduced the alanine aminotransferase and aspertate aminotransferase levels in serum induced by radiation;AS-IV treatment also significantly reduced the expression of inflammatory factors tumor necrosis factor alpha and interleukin 6 and antagonized malonaldehyde content and superoxide dismutase activity in liver caused by radiation;in addition,AS-IV treatment can significantly inhibited the positive expression of thioredoxin-interacting protein(TXNIP)and nod-like receptor protein 3(NLRP3)inflammasome in liver tissue after radiation;The expression of TXNIP,NLRP3 inflammasome,apoptosisassociated speck-like protein containing a CARD,cysteinyl aspartate-specific proteinase 1 and interleukin 1beta in the AS-IV prevention group decreased significantly compared to the radiation group.CONCLUSIONS:These findings suggested that Co60γradiation can cause structural and functional damage to the liver,which may be related to the NLRP3 mediated inflammatory pathway;AS-IV may play a protective role by inhibiting the TXNIP/NLRP3 inflammasome signaling pathway in the radiation-induced liver injury model.展开更多
弛豫铁电体因其卓越的介电和压电特性,在传感器、光电器件、高密度存储器、类脑计算等领域展现出广泛的应用潜力。然而,纳米尺度超薄膜的弛豫特性研究受到严重漏电流的限制,基于Sawyer-Tower电路和Positive-Up-Negative-Down(PUND)脉冲...弛豫铁电体因其卓越的介电和压电特性,在传感器、光电器件、高密度存储器、类脑计算等领域展现出广泛的应用潜力。然而,纳米尺度超薄膜的弛豫特性研究受到严重漏电流的限制,基于Sawyer-Tower电路和Positive-Up-Negative-Down(PUND)脉冲波形的测试方法存在显著挑战。本研究提出了一种基于压电力显微镜(Piezoresponse Force Microscopy, PFM)的测试方法,来研究纳米尺度弛豫薄膜的极化特性。以Pb(Mg,Nb)O_(3)-PbTiO_(3)(PMN-PT)超薄膜为例,比较了不同厚度的PMN-PT弛豫薄膜与铁电Pb(Zr,Ti)O_(3)(PZT)薄膜在双频追踪PFM(DART-PFM)测量中On-field和Off-field两种模式下的极化回滞行为。通过调节PFM回线测量中的用于极化读出的交流信号电压振幅,系统表征了纳米厚度PMN-PT薄膜的弛豫特性。进一步对不同面内应变和厚度的PMN-PT超薄膜进行PFM测试,发现在较大压缩应变(3.19%)下,弛豫特性被抑制,表现出显著的铁电特性,并观测到铁电-弛豫转变的临界厚度。这些实验结果验证了所提出测试方法的有效性。本研究不仅为超薄膜弛豫特性的探索提供了一种新的表征方法,也为理解铁电材料的弛豫极化行为奠定了基础,推动了弛豫铁电材料在低维电子学器件中的应用。展开更多
为探究耕作和施肥方式对西北半干旱区饲用玉米(Zea may L.)土壤水分和产量的影响,以饲用玉米陇饲1号为材料,设置传统旋耕、立式深旋耕2种耕作方式以及单施化肥、有机肥替代化肥2种施肥方式组合,共4个处理,研究不同的耕作和施肥方式对饲...为探究耕作和施肥方式对西北半干旱区饲用玉米(Zea may L.)土壤水分和产量的影响,以饲用玉米陇饲1号为材料,设置传统旋耕、立式深旋耕2种耕作方式以及单施化肥、有机肥替代化肥2种施肥方式组合,共4个处理,研究不同的耕作和施肥方式对饲用玉米土壤贮水量、花前花后耗水量、单株鲜重和干重以及产量的影响。结果表明,与传统旋耕相比,立式深旋耕能够降低饲用玉米0~300 cm土层土壤贮水量,提高花前耗水量,降低花后耗水量,增加生育期总耗水量,而有机肥替代化肥能够降低立式深旋耕方式下土壤总耗水量;立式深旋耕使成熟期单株干重增加1.3%~10.6%,单株鲜重增加4.9%~21.9%,而且不同程度增加了饲用玉米株高、穗长、穗粗、行粒数、百粒重、双穗率,降低了秃顶长,以上指标的变化均有利于高产试验形成。3年试验中立式深旋耕化肥处理较其他处理的籽粒产量增加1.8%~38.6%,丰水年生物量增加1.2%~15.1%,立式深旋耕有机肥处理较其他处理提高了干旱年生物量4.9%~21.9%、籽粒产量水分利用效率6.3%~34.8%、生物量水分利用效率7.1%~21.5%。综上,立式深旋耕能够改善作物生长土壤环境,有利于饲用玉米对土壤水分的吸收以及干物质量的积累,其组合化肥处理可以增加饲用玉米籽粒产量和丰水年生物量,组合有机肥替代处理可增加干旱年饲用玉米生物量和水分利用效率。本研究为西北半干旱区饲用玉米高产高效可持续生产提供了理论依据。展开更多
基金Supported by Natural Science Foundation-Funded Project:Molecular Mechanism of the Brain AQP4 Adaptation to the Extreme Hhypoxia in the Qinghai-Tibet Plateau Animal(No.31760271)。
文摘OBJECTIVE:To investigate the efficacy of Astragaloside IV(AS-IV)on radiation-induced liver inflammation in mice.METHODS:The mice were divided into normal group,dimethyl sulfoxide solvent group,irradiation group(IR),irradiation+AS-IV(20 mg/kg)group(IR+AS-20)and irradiation+AS-IV(40 mg/kg)group(IR+AS-40).One month after intraperitoneal injection of AS-IV,the mice were irradiated with 8Gry Co60γ,the blood was collected for biochemical analysis,and the liver was collected for hematoxylin-eosin staining,immunofluorescence and electron microscopic observation,oxidative stress,and Western blot analysis.RESULTS:The AS-IV treatment significantly ameliorated the pathological morphology of liver and reduced the alanine aminotransferase and aspertate aminotransferase levels in serum induced by radiation;AS-IV treatment also significantly reduced the expression of inflammatory factors tumor necrosis factor alpha and interleukin 6 and antagonized malonaldehyde content and superoxide dismutase activity in liver caused by radiation;in addition,AS-IV treatment can significantly inhibited the positive expression of thioredoxin-interacting protein(TXNIP)and nod-like receptor protein 3(NLRP3)inflammasome in liver tissue after radiation;The expression of TXNIP,NLRP3 inflammasome,apoptosisassociated speck-like protein containing a CARD,cysteinyl aspartate-specific proteinase 1 and interleukin 1beta in the AS-IV prevention group decreased significantly compared to the radiation group.CONCLUSIONS:These findings suggested that Co60γradiation can cause structural and functional damage to the liver,which may be related to the NLRP3 mediated inflammatory pathway;AS-IV may play a protective role by inhibiting the TXNIP/NLRP3 inflammasome signaling pathway in the radiation-induced liver injury model.
文摘弛豫铁电体因其卓越的介电和压电特性,在传感器、光电器件、高密度存储器、类脑计算等领域展现出广泛的应用潜力。然而,纳米尺度超薄膜的弛豫特性研究受到严重漏电流的限制,基于Sawyer-Tower电路和Positive-Up-Negative-Down(PUND)脉冲波形的测试方法存在显著挑战。本研究提出了一种基于压电力显微镜(Piezoresponse Force Microscopy, PFM)的测试方法,来研究纳米尺度弛豫薄膜的极化特性。以Pb(Mg,Nb)O_(3)-PbTiO_(3)(PMN-PT)超薄膜为例,比较了不同厚度的PMN-PT弛豫薄膜与铁电Pb(Zr,Ti)O_(3)(PZT)薄膜在双频追踪PFM(DART-PFM)测量中On-field和Off-field两种模式下的极化回滞行为。通过调节PFM回线测量中的用于极化读出的交流信号电压振幅,系统表征了纳米厚度PMN-PT薄膜的弛豫特性。进一步对不同面内应变和厚度的PMN-PT超薄膜进行PFM测试,发现在较大压缩应变(3.19%)下,弛豫特性被抑制,表现出显著的铁电特性,并观测到铁电-弛豫转变的临界厚度。这些实验结果验证了所提出测试方法的有效性。本研究不仅为超薄膜弛豫特性的探索提供了一种新的表征方法,也为理解铁电材料的弛豫极化行为奠定了基础,推动了弛豫铁电材料在低维电子学器件中的应用。
文摘为探究耕作和施肥方式对西北半干旱区饲用玉米(Zea may L.)土壤水分和产量的影响,以饲用玉米陇饲1号为材料,设置传统旋耕、立式深旋耕2种耕作方式以及单施化肥、有机肥替代化肥2种施肥方式组合,共4个处理,研究不同的耕作和施肥方式对饲用玉米土壤贮水量、花前花后耗水量、单株鲜重和干重以及产量的影响。结果表明,与传统旋耕相比,立式深旋耕能够降低饲用玉米0~300 cm土层土壤贮水量,提高花前耗水量,降低花后耗水量,增加生育期总耗水量,而有机肥替代化肥能够降低立式深旋耕方式下土壤总耗水量;立式深旋耕使成熟期单株干重增加1.3%~10.6%,单株鲜重增加4.9%~21.9%,而且不同程度增加了饲用玉米株高、穗长、穗粗、行粒数、百粒重、双穗率,降低了秃顶长,以上指标的变化均有利于高产试验形成。3年试验中立式深旋耕化肥处理较其他处理的籽粒产量增加1.8%~38.6%,丰水年生物量增加1.2%~15.1%,立式深旋耕有机肥处理较其他处理提高了干旱年生物量4.9%~21.9%、籽粒产量水分利用效率6.3%~34.8%、生物量水分利用效率7.1%~21.5%。综上,立式深旋耕能够改善作物生长土壤环境,有利于饲用玉米对土壤水分的吸收以及干物质量的积累,其组合化肥处理可以增加饲用玉米籽粒产量和丰水年生物量,组合有机肥替代处理可增加干旱年饲用玉米生物量和水分利用效率。本研究为西北半干旱区饲用玉米高产高效可持续生产提供了理论依据。
