OBJECTIVE:To investigate the efficacy of Astragaloside IV(AS-IV)on radiation-induced liver inflammation in mice.METHODS:The mice were divided into normal group,dimethyl sulfoxide solvent group,irradiation group(IR),ir...OBJECTIVE:To investigate the efficacy of Astragaloside IV(AS-IV)on radiation-induced liver inflammation in mice.METHODS:The mice were divided into normal group,dimethyl sulfoxide solvent group,irradiation group(IR),irradiation+AS-IV(20 mg/kg)group(IR+AS-20)and irradiation+AS-IV(40 mg/kg)group(IR+AS-40).One month after intraperitoneal injection of AS-IV,the mice were irradiated with 8Gry Co60γ,the blood was collected for biochemical analysis,and the liver was collected for hematoxylin-eosin staining,immunofluorescence and electron microscopic observation,oxidative stress,and Western blot analysis.RESULTS:The AS-IV treatment significantly ameliorated the pathological morphology of liver and reduced the alanine aminotransferase and aspertate aminotransferase levels in serum induced by radiation;AS-IV treatment also significantly reduced the expression of inflammatory factors tumor necrosis factor alpha and interleukin 6 and antagonized malonaldehyde content and superoxide dismutase activity in liver caused by radiation;in addition,AS-IV treatment can significantly inhibited the positive expression of thioredoxin-interacting protein(TXNIP)and nod-like receptor protein 3(NLRP3)inflammasome in liver tissue after radiation;The expression of TXNIP,NLRP3 inflammasome,apoptosisassociated speck-like protein containing a CARD,cysteinyl aspartate-specific proteinase 1 and interleukin 1beta in the AS-IV prevention group decreased significantly compared to the radiation group.CONCLUSIONS:These findings suggested that Co60γradiation can cause structural and functional damage to the liver,which may be related to the NLRP3 mediated inflammatory pathway;AS-IV may play a protective role by inhibiting the TXNIP/NLRP3 inflammasome signaling pathway in the radiation-induced liver injury model.展开更多
弛豫铁电体因其卓越的介电和压电特性,在传感器、光电器件、高密度存储器、类脑计算等领域展现出广泛的应用潜力。然而,纳米尺度超薄膜的弛豫特性研究受到严重漏电流的限制,基于Sawyer-Tower电路和Positive-Up-Negative-Down(PUND)脉冲...弛豫铁电体因其卓越的介电和压电特性,在传感器、光电器件、高密度存储器、类脑计算等领域展现出广泛的应用潜力。然而,纳米尺度超薄膜的弛豫特性研究受到严重漏电流的限制,基于Sawyer-Tower电路和Positive-Up-Negative-Down(PUND)脉冲波形的测试方法存在显著挑战。本研究提出了一种基于压电力显微镜(Piezoresponse Force Microscopy, PFM)的测试方法,来研究纳米尺度弛豫薄膜的极化特性。以Pb(Mg,Nb)O_(3)-PbTiO_(3)(PMN-PT)超薄膜为例,比较了不同厚度的PMN-PT弛豫薄膜与铁电Pb(Zr,Ti)O_(3)(PZT)薄膜在双频追踪PFM(DART-PFM)测量中On-field和Off-field两种模式下的极化回滞行为。通过调节PFM回线测量中的用于极化读出的交流信号电压振幅,系统表征了纳米厚度PMN-PT薄膜的弛豫特性。进一步对不同面内应变和厚度的PMN-PT超薄膜进行PFM测试,发现在较大压缩应变(3.19%)下,弛豫特性被抑制,表现出显著的铁电特性,并观测到铁电-弛豫转变的临界厚度。这些实验结果验证了所提出测试方法的有效性。本研究不仅为超薄膜弛豫特性的探索提供了一种新的表征方法,也为理解铁电材料的弛豫极化行为奠定了基础,推动了弛豫铁电材料在低维电子学器件中的应用。展开更多
基金Supported by Natural Science Foundation-Funded Project:Molecular Mechanism of the Brain AQP4 Adaptation to the Extreme Hhypoxia in the Qinghai-Tibet Plateau Animal(No.31760271)。
文摘OBJECTIVE:To investigate the efficacy of Astragaloside IV(AS-IV)on radiation-induced liver inflammation in mice.METHODS:The mice were divided into normal group,dimethyl sulfoxide solvent group,irradiation group(IR),irradiation+AS-IV(20 mg/kg)group(IR+AS-20)and irradiation+AS-IV(40 mg/kg)group(IR+AS-40).One month after intraperitoneal injection of AS-IV,the mice were irradiated with 8Gry Co60γ,the blood was collected for biochemical analysis,and the liver was collected for hematoxylin-eosin staining,immunofluorescence and electron microscopic observation,oxidative stress,and Western blot analysis.RESULTS:The AS-IV treatment significantly ameliorated the pathological morphology of liver and reduced the alanine aminotransferase and aspertate aminotransferase levels in serum induced by radiation;AS-IV treatment also significantly reduced the expression of inflammatory factors tumor necrosis factor alpha and interleukin 6 and antagonized malonaldehyde content and superoxide dismutase activity in liver caused by radiation;in addition,AS-IV treatment can significantly inhibited the positive expression of thioredoxin-interacting protein(TXNIP)and nod-like receptor protein 3(NLRP3)inflammasome in liver tissue after radiation;The expression of TXNIP,NLRP3 inflammasome,apoptosisassociated speck-like protein containing a CARD,cysteinyl aspartate-specific proteinase 1 and interleukin 1beta in the AS-IV prevention group decreased significantly compared to the radiation group.CONCLUSIONS:These findings suggested that Co60γradiation can cause structural and functional damage to the liver,which may be related to the NLRP3 mediated inflammatory pathway;AS-IV may play a protective role by inhibiting the TXNIP/NLRP3 inflammasome signaling pathway in the radiation-induced liver injury model.
文摘弛豫铁电体因其卓越的介电和压电特性,在传感器、光电器件、高密度存储器、类脑计算等领域展现出广泛的应用潜力。然而,纳米尺度超薄膜的弛豫特性研究受到严重漏电流的限制,基于Sawyer-Tower电路和Positive-Up-Negative-Down(PUND)脉冲波形的测试方法存在显著挑战。本研究提出了一种基于压电力显微镜(Piezoresponse Force Microscopy, PFM)的测试方法,来研究纳米尺度弛豫薄膜的极化特性。以Pb(Mg,Nb)O_(3)-PbTiO_(3)(PMN-PT)超薄膜为例,比较了不同厚度的PMN-PT弛豫薄膜与铁电Pb(Zr,Ti)O_(3)(PZT)薄膜在双频追踪PFM(DART-PFM)测量中On-field和Off-field两种模式下的极化回滞行为。通过调节PFM回线测量中的用于极化读出的交流信号电压振幅,系统表征了纳米厚度PMN-PT薄膜的弛豫特性。进一步对不同面内应变和厚度的PMN-PT超薄膜进行PFM测试,发现在较大压缩应变(3.19%)下,弛豫特性被抑制,表现出显著的铁电特性,并观测到铁电-弛豫转变的临界厚度。这些实验结果验证了所提出测试方法的有效性。本研究不仅为超薄膜弛豫特性的探索提供了一种新的表征方法,也为理解铁电材料的弛豫极化行为奠定了基础,推动了弛豫铁电材料在低维电子学器件中的应用。
