The kinetic study of in-situ eopolymerization of aniline with o- and p-methylaniline by ammonium persulfate （APS） has been carried out. UV-vis spectroscopic method was used to investigate the course of copolymerizat...The kinetic study of in-situ eopolymerization of aniline with o- and p-methylaniline by ammonium persulfate （APS） has been carried out. UV-vis spectroscopic method was used to investigate the course of copolymerization. Structural characterization was studied by PT-IR spectral analysis. The electronic spectra of the copolymers poly（aniline-co-p-toluidine） and poly（aniline-co-o- toluidine） show blue shift. The shift has been observed in the bands corresponding to π→π^＊ transition as well as in the exciton transition. The increase in absorbance recorded during the reaction for different concentration of aniline, o- and p-toluidine at various intervals of time of polymerization reaction indicates a growth in the polymer formation. The resulting first-order rate constant was used to calculate the rate of copolymer formation using the rate equation -d[A]/dt = kc^n.展开更多
The serological method is one of the most important techniques extensively used in crop production to detect different pathogens,especially plant viruses.An antiserum is essential for serological tests.The 17 kDa move...The serological method is one of the most important techniques extensively used in crop production to detect different pathogens,especially plant viruses.An antiserum is essential for serological tests.The 17 kDa movement protein(MP)of Potato leafroll virus(PLRV)is related to the membranous structures and localized to the plasmodesmata,but there is no report on preparation of PLRV-MP antiserum for detection of PLRV.To prepare PLRV-MP antiserum,reverse transcription polymerase chain reaction(RT-PCR)was carried out to amplify the PLRV-MP gene,which was constructed into a prokaryotic vector to express the protein in Escherichia coli for immunization of rabbits,after purification.Western blotting revealed that this developed antiserum could effectively detect PLRV,but with better results from the perspective of color development and economics by using antiserum at the ratio range of 1:10000 to 1:40000,presenting high sensitivity and specificity to PLRV.The serological detection results for PLRV of the field samples were identical to the RT-PCR detection.This is the first report on the development of PLRV-MP antiserum that has been successfully used for both laboratory and field detection of PLRV.The results provide a fundamental tool for further research on the function of PLRV-MP.展开更多
文摘The kinetic study of in-situ eopolymerization of aniline with o- and p-methylaniline by ammonium persulfate （APS） has been carried out. UV-vis spectroscopic method was used to investigate the course of copolymerization. Structural characterization was studied by PT-IR spectral analysis. The electronic spectra of the copolymers poly（aniline-co-p-toluidine） and poly（aniline-co-o- toluidine） show blue shift. The shift has been observed in the bands corresponding to π→π^＊ transition as well as in the exciton transition. The increase in absorbance recorded during the reaction for different concentration of aniline, o- and p-toluidine at various intervals of time of polymerization reaction indicates a growth in the polymer formation. The resulting first-order rate constant was used to calculate the rate of copolymer formation using the rate equation -d[A]/dt = kc^n.
基金supported by the National Natural Science Foundation of China(31671995 and 31371909)the 111 project(B13006).
文摘The serological method is one of the most important techniques extensively used in crop production to detect different pathogens,especially plant viruses.An antiserum is essential for serological tests.The 17 kDa movement protein(MP)of Potato leafroll virus(PLRV)is related to the membranous structures and localized to the plasmodesmata,but there is no report on preparation of PLRV-MP antiserum for detection of PLRV.To prepare PLRV-MP antiserum,reverse transcription polymerase chain reaction(RT-PCR)was carried out to amplify the PLRV-MP gene,which was constructed into a prokaryotic vector to express the protein in Escherichia coli for immunization of rabbits,after purification.Western blotting revealed that this developed antiserum could effectively detect PLRV,but with better results from the perspective of color development and economics by using antiserum at the ratio range of 1:10000 to 1:40000,presenting high sensitivity and specificity to PLRV.The serological detection results for PLRV of the field samples were identical to the RT-PCR detection.This is the first report on the development of PLRV-MP antiserum that has been successfully used for both laboratory and field detection of PLRV.The results provide a fundamental tool for further research on the function of PLRV-MP.
