Identifying cryptic species poses a substantial challenge to both biologists and naturalists due to morphological similarities.Bemisia tabaci is a cryptic species complex containing more than 44 putative species;sever...Identifying cryptic species poses a substantial challenge to both biologists and naturalists due to morphological similarities.Bemisia tabaci is a cryptic species complex containing more than 44 putative species;several of which are currently among the world's most destructive crop pests.Interpreting and delimiting the evolution of this species complex has proved problematic.To develop a comprehensive framework for species delimitation and identification,we evaluated the performance of distinct data sources both individually and in combination among numerous samples of the B.tabaci species complex acquired worldwide.Distinct datasets include full mitogenomes,single-copy nuclear genes,restriction site-associated DNA sequencing,geographic range,host speciation,and reproductive compatibility datasets.Phylogenetically,our well-supported topologies generated from three dense molecular markers highlighted the evolutionary divergence of species of the B.tabaci complex and suggested that the nuclear markers serve as a more accurate representation of B.tabaci species diversity.Reproductive compatibility datasets facilitated the identification of at least 17 different cryptic species within our samples.Native geographic range information provides a complementary assessment of species recognition,while the host range datasets provide low rate of delimiting resolution.We further summarized different data performances in species classification when compared with reproductive compatibility,indicating that combination of mtCOI divergence,nuclear markers,geographic range provide a complementary assessment of species recognition.Finally,we represent a model for understanding and untangling the cryptic species complexes based on the evidence from this study and previously published articles.展开更多
Bemisia tabaci is a complex of cryptic agro-economically important pest species characterized by diverse clades,substantial genetic diversity along with strong phylogeographic associations.However,a comprehensive phyl...Bemisia tabaci is a complex of cryptic agro-economically important pest species characterized by diverse clades,substantial genetic diversity along with strong phylogeographic associations.However,a comprehensive phylogenomic analysis across the entire complex has been lacking,we thus conducted phylogenomic analyses and explored biogeographic patterns using 680 single-copy nuclear genes(SCNs)obtained from whole-genome sequencing data of 58 globally sourced B.tabaci specimens.We constructed both concatenation and coalescent trees using 680 SCNs,which produced highly supported bootstrap values and nearly identical topologies for all major clades.When comparing these concatenation trees with those constructed using mitochondrial cytochrome oxidase I(mtCOI)and mitochondrial genome,we found conflicting phylogenetic relationships,with the later trees recovering fewer major clades.In a separate comparison between concatenation and coalescent trees,particularly those generated using IQ-TREE,they were found to delineate population relationships more effectively than RaxML.In contrast,coalescent phylogenies were proficient in elucidating geographical dispersal patterns and the reorganization of biological species.Furthermore,we provided a strict consensus tree that clearly defines relationships within most clades,laying a solid foundation for future research on the evolution and taxonomy of B.tabaci.Ancestral range estimates suggested that the ancestral region of the complex is likely situated in equatorial Africa,the Middle East,and Mediterranean regions.Subsequently,the expansion occurred into part of the Palearctic and further into the Nearctic,Neotropical,Indomalayan,and Australasian regions.These findings challenge both previous classifications and origin hypotheses,offering a notably more comprehensive understanding of the global distribution,evolutionary history,diversification,and biogeography of B.tabaci.展开更多
基金Peter Sseruwagi and Joseph Ndunguru,Mikocheni Agriculture Research Institute,Tanzania,for collecting samples from Uganda.J.N.C.is supported by grants(AGL2013-48913-C2-1-R and AGL2016-75819-C2-2-R)from the Ministerio de Economia y Competitividad(MINECO,Spain)co-financed by ERDF.E.F.O.is a recipient of a"Juan de la Cierva-Incorporacion"postdoctoral contract from MINECO.
文摘Identifying cryptic species poses a substantial challenge to both biologists and naturalists due to morphological similarities.Bemisia tabaci is a cryptic species complex containing more than 44 putative species;several of which are currently among the world's most destructive crop pests.Interpreting and delimiting the evolution of this species complex has proved problematic.To develop a comprehensive framework for species delimitation and identification,we evaluated the performance of distinct data sources both individually and in combination among numerous samples of the B.tabaci species complex acquired worldwide.Distinct datasets include full mitogenomes,single-copy nuclear genes,restriction site-associated DNA sequencing,geographic range,host speciation,and reproductive compatibility datasets.Phylogenetically,our well-supported topologies generated from three dense molecular markers highlighted the evolutionary divergence of species of the B.tabaci complex and suggested that the nuclear markers serve as a more accurate representation of B.tabaci species diversity.Reproductive compatibility datasets facilitated the identification of at least 17 different cryptic species within our samples.Native geographic range information provides a complementary assessment of species recognition,while the host range datasets provide low rate of delimiting resolution.We further summarized different data performances in species classification when compared with reproductive compatibility,indicating that combination of mtCOI divergence,nuclear markers,geographic range provide a complementary assessment of species recognition.Finally,we represent a model for understanding and untangling the cryptic species complexes based on the evidence from this study and previously published articles.
基金supported by the Bill&Melinda Gates Foundation(African cassava whitefly project,OPP1058938)the National Natural Science Foundation of China(Grant numbers 31501878).
文摘Bemisia tabaci is a complex of cryptic agro-economically important pest species characterized by diverse clades,substantial genetic diversity along with strong phylogeographic associations.However,a comprehensive phylogenomic analysis across the entire complex has been lacking,we thus conducted phylogenomic analyses and explored biogeographic patterns using 680 single-copy nuclear genes(SCNs)obtained from whole-genome sequencing data of 58 globally sourced B.tabaci specimens.We constructed both concatenation and coalescent trees using 680 SCNs,which produced highly supported bootstrap values and nearly identical topologies for all major clades.When comparing these concatenation trees with those constructed using mitochondrial cytochrome oxidase I(mtCOI)and mitochondrial genome,we found conflicting phylogenetic relationships,with the later trees recovering fewer major clades.In a separate comparison between concatenation and coalescent trees,particularly those generated using IQ-TREE,they were found to delineate population relationships more effectively than RaxML.In contrast,coalescent phylogenies were proficient in elucidating geographical dispersal patterns and the reorganization of biological species.Furthermore,we provided a strict consensus tree that clearly defines relationships within most clades,laying a solid foundation for future research on the evolution and taxonomy of B.tabaci.Ancestral range estimates suggested that the ancestral region of the complex is likely situated in equatorial Africa,the Middle East,and Mediterranean regions.Subsequently,the expansion occurred into part of the Palearctic and further into the Nearctic,Neotropical,Indomalayan,and Australasian regions.These findings challenge both previous classifications and origin hypotheses,offering a notably more comprehensive understanding of the global distribution,evolutionary history,diversification,and biogeography of B.tabaci.
