用分级法和^(13)C-NMR研究了从中国产江蓠属红藻(真江蓠、细基江蓠、芋根江蓠和凤尾菜)提取的6份琼胶多糖的结构特征。这些琼胶主要由用0.5mol/L和1.0mol/LNaCl从DEAE-Sephadex A 50层析柱洗脱下的带电荷的琼胶糖分子组成。6.0mol/L尿...用分级法和^(13)C-NMR研究了从中国产江蓠属红藻(真江蓠、细基江蓠、芋根江蓠和凤尾菜)提取的6份琼胶多糖的结构特征。这些琼胶主要由用0.5mol/L和1.0mol/LNaCl从DEAE-Sephadex A 50层析柱洗脱下的带电荷的琼胶糖分子组成。6.0mol/L尿素级分是带低电荷密度的琼胶糖分子。各级分总得率达80%或以上。^(13)C-NMR谱图表明各种江蓠琼胶的主要级分基本上由琼胶糖结构构成,但有的级分中琼胶糖含微量6-SO_(4)-L-半乳糖,真江蓠和细基江蓠的琼胶糖含有6-OCH_(3)-D-半乳糖,而凤尾菜琼胶中则主要含有2-OCH_(3)-3,6-内醚-L-半乳糖。真江蓠和凤尾菜的个别级分的琼胶酶降解产物与^(13)C-NMR分析结果相一致。展开更多
The 60% ethanolic extract from Gracilaria textorii (Rhbdophyta) was degraded with B- agarase, and certain charged (sulfated) and neutral oligosaccharides were separated by using DEAE Sephadex A 25 and Bio- gel P6 , P2...The 60% ethanolic extract from Gracilaria textorii (Rhbdophyta) was degraded with B- agarase, and certain charged (sulfated) and neutral oligosaccharides were separated by using DEAE Sephadex A 25 and Bio- gel P6 , P2 chromatographic techniques . Some of the charged oligomers were verified to be neoagarotetraose - 63- sulfate ( DP2 ). neoagarohexaose- 63 】 65 - disulfate ( DP3 ) and neoagarooctaose- 63, 65, 67-trisulfate (DP4) by using 13C-and ’H-NMR spectroscopy . One neutral oligomer was assumed to be a mixture of methylated neoagarotetraoses (DP2 ) by 1H-NMR spectroscopy .These oligomers assigned by their chemical shifts may be used as the model compounds for the structural investigation of the agar- type sulfated polysaccharides using the B- agarase degradation method .展开更多
文摘用分级法和^(13)C-NMR研究了从中国产江蓠属红藻(真江蓠、细基江蓠、芋根江蓠和凤尾菜)提取的6份琼胶多糖的结构特征。这些琼胶主要由用0.5mol/L和1.0mol/LNaCl从DEAE-Sephadex A 50层析柱洗脱下的带电荷的琼胶糖分子组成。6.0mol/L尿素级分是带低电荷密度的琼胶糖分子。各级分总得率达80%或以上。^(13)C-NMR谱图表明各种江蓠琼胶的主要级分基本上由琼胶糖结构构成,但有的级分中琼胶糖含微量6-SO_(4)-L-半乳糖,真江蓠和细基江蓠的琼胶糖含有6-OCH_(3)-D-半乳糖,而凤尾菜琼胶中则主要含有2-OCH_(3)-3,6-内醚-L-半乳糖。真江蓠和凤尾菜的个别级分的琼胶酶降解产物与^(13)C-NMR分析结果相一致。
文摘The 60% ethanolic extract from Gracilaria textorii (Rhbdophyta) was degraded with B- agarase, and certain charged (sulfated) and neutral oligosaccharides were separated by using DEAE Sephadex A 25 and Bio- gel P6 , P2 chromatographic techniques . Some of the charged oligomers were verified to be neoagarotetraose - 63- sulfate ( DP2 ). neoagarohexaose- 63 】 65 - disulfate ( DP3 ) and neoagarooctaose- 63, 65, 67-trisulfate (DP4) by using 13C-and ’H-NMR spectroscopy . One neutral oligomer was assumed to be a mixture of methylated neoagarotetraoses (DP2 ) by 1H-NMR spectroscopy .These oligomers assigned by their chemical shifts may be used as the model compounds for the structural investigation of the agar- type sulfated polysaccharides using the B- agarase degradation method .
