This study concerns the synthesis of gel materials based on carbon nanotubes dispersed strontium-modified hydroxyapatite(Sr-HA)at different compositions obtained by sol–gel technology and their influence on human-bon...This study concerns the synthesis of gel materials based on carbon nanotubes dispersed strontium-modified hydroxyapatite(Sr-HA)at different compositions obtained by sol–gel technology and their influence on human-bone-marrow-derived mesenchymal stem cells.Furthermore,an evaluation of the influence of nanotubes and Strontium on physico-chemical,morphological,rheological and biological properties of hydroxyapatite gel was also performed.Morphological analysis(scanning electron microscopy)shows a homogeneous distribution of modified nanotubes in the ceramic matrix improving the bioactive properties of materials.The biological investigations proved that Sr-HA/carbon nanotube gel containing 0–20 mol(%)of Sr showed no toxic effect and promote the expression of early and late markers of osteogenic differentiation in cell culture performed in basal medium without osteogenic factors.Finally,the SrHA/carbon nanotube gels could have a good potential application as filler in bone repair and regeneration and may be used in the osteoporotic disease treatment.展开更多
The main driving idea of the present study was the comparison between two different chemical modifications of hyaluronic acid(HA)followed by the development of nanocomposite hydrogels directly in situ by biomineraliza...The main driving idea of the present study was the comparison between two different chemical modifications of hyaluronic acid(HA)followed by the development of nanocomposite hydrogels directly in situ by biomineralization of photocrosslinkable HA polymers through sol-gel synthesis.In this way,it has been possible to overcome some limitations due to classical approaches based on the physical blending of inorganic fillers into polymer matrix.To this aim,methacrylated and maleated HA,synthesized with similar degree of substitution(DS)were compared in terms of mechanical and physico-chemical properties.The success of in situ biomineralization was highlighted by reflect Fourier transform infrared spectroscopy and thermogravimetric analysis.Furthermore,mechanical characterization demonstrated the reinforcing effect of inorganic fillers evidencing a strong correlation with DS.The swelling behavior resulted to be correlated with filler concentration.Finally,the cytotoxicity tests revealed the absence of toxic components and an increase of cell proliferation over culture time was observed,highlighting these bio-nanocomposite hyaluronan derivatives as biocompatible hydrogel with tunable properties.展开更多
基金supported through funds provided by the PNR-CNR Aging Program 2012-2014.
文摘This study concerns the synthesis of gel materials based on carbon nanotubes dispersed strontium-modified hydroxyapatite(Sr-HA)at different compositions obtained by sol–gel technology and their influence on human-bone-marrow-derived mesenchymal stem cells.Furthermore,an evaluation of the influence of nanotubes and Strontium on physico-chemical,morphological,rheological and biological properties of hydroxyapatite gel was also performed.Morphological analysis(scanning electron microscopy)shows a homogeneous distribution of modified nanotubes in the ceramic matrix improving the bioactive properties of materials.The biological investigations proved that Sr-HA/carbon nanotube gel containing 0–20 mol(%)of Sr showed no toxic effect and promote the expression of early and late markers of osteogenic differentiation in cell culture performed in basal medium without osteogenic factors.Finally,the SrHA/carbon nanotube gels could have a good potential application as filler in bone repair and regeneration and may be used in the osteoporotic disease treatment.
基金the H2020-MSCA-RISE-2016,SECOND.R.I.,Grant Agreement No 734391Progetto Premiale di AREA SCIENCE PARK“OPEN LAB-A System of Open Research Facilities”for the support。
文摘The main driving idea of the present study was the comparison between two different chemical modifications of hyaluronic acid(HA)followed by the development of nanocomposite hydrogels directly in situ by biomineralization of photocrosslinkable HA polymers through sol-gel synthesis.In this way,it has been possible to overcome some limitations due to classical approaches based on the physical blending of inorganic fillers into polymer matrix.To this aim,methacrylated and maleated HA,synthesized with similar degree of substitution(DS)were compared in terms of mechanical and physico-chemical properties.The success of in situ biomineralization was highlighted by reflect Fourier transform infrared spectroscopy and thermogravimetric analysis.Furthermore,mechanical characterization demonstrated the reinforcing effect of inorganic fillers evidencing a strong correlation with DS.The swelling behavior resulted to be correlated with filler concentration.Finally,the cytotoxicity tests revealed the absence of toxic components and an increase of cell proliferation over culture time was observed,highlighting these bio-nanocomposite hyaluronan derivatives as biocompatible hydrogel with tunable properties.
