<span style="font-family:Verdana;"></span><span style="font-family:Verdana;">This study aimed to describe the factors associated with biofilms formation in dental pathology by com...<span style="font-family:Verdana;"></span><span style="font-family:Verdana;">This study aimed to describe the factors associated with biofilms formation in dental pathology by comparison of bacterial growth on dental and stainless-steel surfaces.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">We studied </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> the behavior of </span><i><span style="font-family:Verdana;">Staphylococcus aureus Méti</span></i><span style="font-family:Verdana;">s in order to observe the capacity of adhesion, to evaluate quantitatively the potential of proliferation and to compare the behavior of this germ in contact with the two surfaces.</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">The biomaterials used were cylinders in Stainless steel (AISI 316L), dental fragments and stainless-steel fragments, all were disinfected for 15 minutes and then sterilized in a wet autoclave at 120<span style="white-space:nowrap;">˚</span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">C for 30 min. Macroscopic observation with a binocular magnifier of bacterial proliferation was carried out regularly after 6 h and 24 h of incubation. Observation by optical microscope based on GRAM staining made it possible to visualize the presence or absence of bacteria and to differentiate them. The adhesion of </span><i><span style="font-family:Verdana;">Staphylococcus aureus Méti S</span></i><span style="font-family:Verdana;"> on dental fragments was compared to </span><span style="font-family:Verdana;">the one obtained on stainless steel fragments. We also carried a Bacterial</span><span style="font-family:Verdana;"> count by optical dosing.</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">The results show that the ability of this germ to colonize and develop biofilms on surfaces depends mainly on the characteristics of the surface. Rough surfaces as dental surface are more likely to developing biofilms than smooth surfaces like stainless-steel surface.</span>展开更多
文摘<span style="font-family:Verdana;"></span><span style="font-family:Verdana;">This study aimed to describe the factors associated with biofilms formation in dental pathology by comparison of bacterial growth on dental and stainless-steel surfaces.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">We studied </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> the behavior of </span><i><span style="font-family:Verdana;">Staphylococcus aureus Méti</span></i><span style="font-family:Verdana;">s in order to observe the capacity of adhesion, to evaluate quantitatively the potential of proliferation and to compare the behavior of this germ in contact with the two surfaces.</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">The biomaterials used were cylinders in Stainless steel (AISI 316L), dental fragments and stainless-steel fragments, all were disinfected for 15 minutes and then sterilized in a wet autoclave at 120<span style="white-space:nowrap;">˚</span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">C for 30 min. Macroscopic observation with a binocular magnifier of bacterial proliferation was carried out regularly after 6 h and 24 h of incubation. Observation by optical microscope based on GRAM staining made it possible to visualize the presence or absence of bacteria and to differentiate them. The adhesion of </span><i><span style="font-family:Verdana;">Staphylococcus aureus Méti S</span></i><span style="font-family:Verdana;"> on dental fragments was compared to </span><span style="font-family:Verdana;">the one obtained on stainless steel fragments. We also carried a Bacterial</span><span style="font-family:Verdana;"> count by optical dosing.</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">The results show that the ability of this germ to colonize and develop biofilms on surfaces depends mainly on the characteristics of the surface. Rough surfaces as dental surface are more likely to developing biofilms than smooth surfaces like stainless-steel surface.</span>
