[Objectives]To establish an efficient and environmentally friendly separation and purification method for the large-scale preparation of the major active components-eleutherol,eleutherine,and isoeleutherine-from the e...[Objectives]To establish an efficient and environmentally friendly separation and purification method for the large-scale preparation of the major active components-eleutherol,eleutherine,and isoeleutherine-from the ethnomedicinal plant Eleutherine americana Merr.et K.Heyne.[Methods]The sample of E.americana bulbs was initially extracted with ethanol,followed by three successive extractions with ethyl acetate-water(2:1,V/V)to obtain the target component-enriched fraction.Eight solvent systems were systematically optimized,and a mixture of petroleum ether-ethyl acetate-ethanol-water(5:5:6:4,V/V/V/V)was identified as the optimal solvent system for high-speed counter-current chromatography(HSCCC)separation under conditions of 900 rpm,2 mL/min,and 35℃.The crude HSCCC product was further purified by silica gel column chromatography(200-300 mesh)using gradient elution with a solvent system of n-hexane-dichloromethane-ethyl acetate(varying from 10:5:1 to 4:5:1,V/V/V).UPLC-PDA(Agilent SB-C_(18)column)and nuclear magnetic resonance spectroscopy(600 MHz)were comprehensively employed to assess compound purity and confirm molecular structures.[Results]An optimized technique integrating HSCCC and silica gel column chromatography was established,successfully enabling the large-scale preparation of three bioactive components:eleutherol(purity 99%),eleutherine(purity 98%),and isoeleutherine(purity 98%).Structural identification results were consistent with those reported in the literature.Compared to traditional methods,the new approach demonstrated improved separation efficiency and reduced solvent consumption.[Conclusions]The combined separation method utilizing HSCCC and silica gel column chromatography established in this study demonstrates notable advantages,including high efficiency,environmental friendliness,and cost-effectiveness,enabling the large-scale preparation of the three major active components from E.americana.This approach outperforms conventional methods by offering higher separation efficiency,reduced solvent consumption,and superior product purity,providing a robust technical solution for the development and utilization of bioactive compounds from E.americana.Moreover,it offers a novel methodological reference for the isolation and purification of other natural products.展开更多
[Objectives]To prepare plumbagin nanomicelle(PLB-N)in-situ gel,and optimize the formulation and process.[Methods]PLB-N was prepared by self-assembly method,and the optimal formulation of PLB-N in-situ gel was determin...[Objectives]To prepare plumbagin nanomicelle(PLB-N)in-situ gel,and optimize the formulation and process.[Methods]PLB-N was prepared by self-assembly method,and the optimal formulation of PLB-N in-situ gel was determined by orthogonal experiment design and single factor method.[Results]The optimal preparation process for PLB-N was a drug to lipid ratio of 1:3,a Tween 80 content of 5%,an ethanol content of 7.5%of the hydration medium,a magnetic stirring speed of 2200 rpm,a stirring time of 30 min,and an ultrasound time of 10 min.The optimal formulation of PLB-N in-situ gel was 22%of poloxamer 407,6%of poloxamer 188,and 1:1 of PLB-N to water.The encapsulation efficiency of PLB-N prepared with the optimal formula was(95.8%±0.4%),and the average particle size was(75.19±1.14)nm,and the Zeta potential was(-20.73±1.19)mv.[Conclusions]PLB-N in-situ gel had stable and reliable preparation process,uniform content,and broad application prospects.展开更多
[Objectives]This study aimed to study and analyze the main components of the oil from seeds of 8 different pomegranate varieties.[Methods]The oil in sour and sweet pomegranate seeds was extracted by ultrasonic-assiste...[Objectives]This study aimed to study and analyze the main components of the oil from seeds of 8 different pomegranate varieties.[Methods]The oil in sour and sweet pomegranate seeds was extracted by ultrasonic-assisted method,and the composition of the soil after methyl esterification was identified by GC-MS.[Results]The oil yield of the four sweet pomegranate varieties and the four sour pomegranate varieties ranged from 9.43%-16.97%.A total of 6 compounds were identified in the methyl esterified pomegranate seed oil by GC-MS.[Conclusions]There were no significant differences in oil yield between sweet and sour pomegranate varieties(P>0.05).The content of methyl punicate in the four sweet pomegranate varieties was 52.18%-78.06%,and that in the four sour pomegranate varieties was 78.25%-84.55%.The content of methyl punicate in sour pomegranate was slightly higher than that in sweet pomegranate.展开更多
[Objectives]The paper was to establish the quality standard of Carpesium lipskyi,and to provide a scientific reference for its quality control and rational development and utilization.[Methods]Morphological identifica...[Objectives]The paper was to establish the quality standard of Carpesium lipskyi,and to provide a scientific reference for its quality control and rational development and utilization.[Methods]Morphological identification,microscopic identification and thin layer chromatography(TLC)identification of the herbal samples were performed.The contents of moisture,total ash,acid-insoluble ash and alcohol-soluble extract of the herbal samples were determined,and their total flavonoid content was determined by UV-spectrophotometry.[Results]The average contents of moisture,total ash,acid-insoluble ash and alcohol-soluble extracts of 10 batches of C.lipskyi were 10.38%-12.27%,8.03%-14.72%,1.01%-3.06%and 20.84%-26.82%,respectively.The total flavonoids content ranged from 4.60%to 5.63%.[Conclusions]The established standard can provide a reference for the quality control of C.lipskyi.The tentative regulations stipulate that the moisture content of C.lipskyi shall not exceed 13.0%;the total ash content shall not exceed 15.0%;the acid-insoluble ash content shall not exceed 4.0%;the alcohol-soluble extract shall not be less than 16.0%;and the total flavonoids shall not be less than 3.0%.展开更多
[Objectives] To study in vitro antioxidant activity of extracts from Eleutherine plicata Herb by different extraction methods.[Methods]1,1-diphenyl-2-picrylhydrazyl( DPPH) free radical scavenging method,2,2'-Azino...[Objectives] To study in vitro antioxidant activity of extracts from Eleutherine plicata Herb by different extraction methods.[Methods]1,1-diphenyl-2-picrylhydrazyl( DPPH) free radical scavenging method,2,2'-Azinobis( 3-ehtylbenzothiazolin-6-sulfnic acid) diammonium salt( ABTS) free radical scavenging method,and iron ion reduction ability determination method were applied to measure the extracts of Eleutherine plicata Herb by ethanol extraction,water extraction,and ethanol extraction after water extraction,and comparison was made with positive control Vc( vitamin C). [Results]The ethanol extract,water extract,and ethanol extract after water extraction of Eleutherine plicata Herb had a high DPPH free radical,ABTS free radical scavenging activity,and reducing activity. [Conclusions]E. plicata Herb is a natural antioxidant with high potential.展开更多
[Objectives] To study the original plant,traits and microscopic identification for Yi medicinal material Campanula colorata,and provide experimental data for its identification and medicinal use. [Methods] Plant ident...[Objectives] To study the original plant,traits and microscopic identification for Yi medicinal material Campanula colorata,and provide experimental data for its identification and medicinal use. [Methods] Plant identification,trait identification,powder identification and paraffin slice identification were adopted in the study. [Results]It was found that the original plant of Campanula colorata was characterized by stiff hairs,slightly thicker root than stem,serrated leaf margin,tubular bell-shaped corolla and blue-purple color; traits were featured by odorlessness,and bitter taste after slight sweetness; the microscopic identification characteristics were that the laticifer was sporadic,containing reddish brown or yellow brown materials,and the oil droplets and nonglandular hairs were common. [Conclusions] The original plant,trait and microscopic identification characteristics were obvious for Yi medicinal material Campanula colorata,which could provide a reference for developing quality standards and identifying the original plant of Campanula colorata,and provide the basis for further research and development.展开更多
基金Supported by Natural Science Foundation of Tibet Autonomous Region,Science and Technology Department of Tibet(XZ202501ZR0118).
文摘[Objectives]To establish an efficient and environmentally friendly separation and purification method for the large-scale preparation of the major active components-eleutherol,eleutherine,and isoeleutherine-from the ethnomedicinal plant Eleutherine americana Merr.et K.Heyne.[Methods]The sample of E.americana bulbs was initially extracted with ethanol,followed by three successive extractions with ethyl acetate-water(2:1,V/V)to obtain the target component-enriched fraction.Eight solvent systems were systematically optimized,and a mixture of petroleum ether-ethyl acetate-ethanol-water(5:5:6:4,V/V/V/V)was identified as the optimal solvent system for high-speed counter-current chromatography(HSCCC)separation under conditions of 900 rpm,2 mL/min,and 35℃.The crude HSCCC product was further purified by silica gel column chromatography(200-300 mesh)using gradient elution with a solvent system of n-hexane-dichloromethane-ethyl acetate(varying from 10:5:1 to 4:5:1,V/V/V).UPLC-PDA(Agilent SB-C_(18)column)and nuclear magnetic resonance spectroscopy(600 MHz)were comprehensively employed to assess compound purity and confirm molecular structures.[Results]An optimized technique integrating HSCCC and silica gel column chromatography was established,successfully enabling the large-scale preparation of three bioactive components:eleutherol(purity 99%),eleutherine(purity 98%),and isoeleutherine(purity 98%).Structural identification results were consistent with those reported in the literature.Compared to traditional methods,the new approach demonstrated improved separation efficiency and reduced solvent consumption.[Conclusions]The combined separation method utilizing HSCCC and silica gel column chromatography established in this study demonstrates notable advantages,including high efficiency,environmental friendliness,and cost-effectiveness,enabling the large-scale preparation of the three major active components from E.americana.This approach outperforms conventional methods by offering higher separation efficiency,reduced solvent consumption,and superior product purity,providing a robust technical solution for the development and utilization of bioactive compounds from E.americana.Moreover,it offers a novel methodological reference for the isolation and purification of other natural products.
基金Supported by Special Fund for Basic Scientific Research Business in Central Universities(2019NYB31)Scientific Research Funded Project of Southwest Minzu University(2023KYZZ06N).
文摘[Objectives]To prepare plumbagin nanomicelle(PLB-N)in-situ gel,and optimize the formulation and process.[Methods]PLB-N was prepared by self-assembly method,and the optimal formulation of PLB-N in-situ gel was determined by orthogonal experiment design and single factor method.[Results]The optimal preparation process for PLB-N was a drug to lipid ratio of 1:3,a Tween 80 content of 5%,an ethanol content of 7.5%of the hydration medium,a magnetic stirring speed of 2200 rpm,a stirring time of 30 min,and an ultrasound time of 10 min.The optimal formulation of PLB-N in-situ gel was 22%of poloxamer 407,6%of poloxamer 188,and 1:1 of PLB-N to water.The encapsulation efficiency of PLB-N prepared with the optimal formula was(95.8%±0.4%),and the average particle size was(75.19±1.14)nm,and the Zeta potential was(-20.73±1.19)mv.[Conclusions]PLB-N in-situ gel had stable and reliable preparation process,uniform content,and broad application prospects.
基金the Program of Study Abroad for Young Scholar Sponsored by China Scholarship Council(CSC201800850005)Central University Basic Research Funds Project(No.2019NYB31).
文摘[Objectives]This study aimed to study and analyze the main components of the oil from seeds of 8 different pomegranate varieties.[Methods]The oil in sour and sweet pomegranate seeds was extracted by ultrasonic-assisted method,and the composition of the soil after methyl esterification was identified by GC-MS.[Results]The oil yield of the four sweet pomegranate varieties and the four sour pomegranate varieties ranged from 9.43%-16.97%.A total of 6 compounds were identified in the methyl esterified pomegranate seed oil by GC-MS.[Conclusions]There were no significant differences in oil yield between sweet and sour pomegranate varieties(P>0.05).The content of methyl punicate in the four sweet pomegranate varieties was 52.18%-78.06%,and that in the four sour pomegranate varieties was 78.25%-84.55%.The content of methyl punicate in sour pomegranate was slightly higher than that in sweet pomegranate.
基金Supported by Traditional Chinese Medicine(Ethnic Medicine)Standard Improvement Project of Sichuan Medical Products Administration(510201202102305)Scientific Research Funded Project of Southwest Minzu University(2023KYZZ06N)。
文摘[Objectives]The paper was to establish the quality standard of Carpesium lipskyi,and to provide a scientific reference for its quality control and rational development and utilization.[Methods]Morphological identification,microscopic identification and thin layer chromatography(TLC)identification of the herbal samples were performed.The contents of moisture,total ash,acid-insoluble ash and alcohol-soluble extract of the herbal samples were determined,and their total flavonoid content was determined by UV-spectrophotometry.[Results]The average contents of moisture,total ash,acid-insoluble ash and alcohol-soluble extracts of 10 batches of C.lipskyi were 10.38%-12.27%,8.03%-14.72%,1.01%-3.06%and 20.84%-26.82%,respectively.The total flavonoids content ranged from 4.60%to 5.63%.[Conclusions]The established standard can provide a reference for the quality control of C.lipskyi.The tentative regulations stipulate that the moisture content of C.lipskyi shall not exceed 13.0%;the total ash content shall not exceed 15.0%;the acid-insoluble ash content shall not exceed 4.0%;the alcohol-soluble extract shall not be less than 16.0%;and the total flavonoids shall not be less than 3.0%.
基金Supported by Science and Technology Project of Sichuan Province(2015-JY0226)
文摘[Objectives] To study in vitro antioxidant activity of extracts from Eleutherine plicata Herb by different extraction methods.[Methods]1,1-diphenyl-2-picrylhydrazyl( DPPH) free radical scavenging method,2,2'-Azinobis( 3-ehtylbenzothiazolin-6-sulfnic acid) diammonium salt( ABTS) free radical scavenging method,and iron ion reduction ability determination method were applied to measure the extracts of Eleutherine plicata Herb by ethanol extraction,water extraction,and ethanol extraction after water extraction,and comparison was made with positive control Vc( vitamin C). [Results]The ethanol extract,water extract,and ethanol extract after water extraction of Eleutherine plicata Herb had a high DPPH free radical,ABTS free radical scavenging activity,and reducing activity. [Conclusions]E. plicata Herb is a natural antioxidant with high potential.
基金Supported by National Science and Technology Support Program(2015BAC05-B02)Sichuan Science and Technology Support Program(2015SZ0034)+1 种基金Education and Teaching Research and Reform Project of Southwest University for Nationalities(2015)National Development Project for Central Universities-Southwest University for Nationalities(2016NGJPY11)
文摘[Objectives] To study the original plant,traits and microscopic identification for Yi medicinal material Campanula colorata,and provide experimental data for its identification and medicinal use. [Methods] Plant identification,trait identification,powder identification and paraffin slice identification were adopted in the study. [Results]It was found that the original plant of Campanula colorata was characterized by stiff hairs,slightly thicker root than stem,serrated leaf margin,tubular bell-shaped corolla and blue-purple color; traits were featured by odorlessness,and bitter taste after slight sweetness; the microscopic identification characteristics were that the laticifer was sporadic,containing reddish brown or yellow brown materials,and the oil droplets and nonglandular hairs were common. [Conclusions] The original plant,trait and microscopic identification characteristics were obvious for Yi medicinal material Campanula colorata,which could provide a reference for developing quality standards and identifying the original plant of Campanula colorata,and provide the basis for further research and development.
