new heterocyclic dipeptide with a highly functionalized 1,2-oxazadecaline core,named trichodermamide H(1),and three known analogues,along with three known polyketides,were isolated from the fermentation extract of the...new heterocyclic dipeptide with a highly functionalized 1,2-oxazadecaline core,named trichodermamide H(1),and three known analogues,along with three known polyketides,were isolated from the fermentation extract of the mangrovederived fungus Penicillium janthinellum XLN32122.The structure of 1 was elucidated on the basis of extensive 1D and 2D NMR spectra data analysis,HR-ESI-MS,electronic circular dichroism(ECD)calculations.Trichodermamide B(3)exhibited better inhibitory effect on nitric oxide(NO)production in lipopolysaccharide(LPS)induced RAW 264.7 cells with an IC_(50)value of(13.13±0.005)μmol/L than that of the positive control dexamethasone[IC_(50)=(136.84±1.33)μmol/L].Compound 3 exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus(MRSA)with an IC_(50)value of 12.5μg/mL,while the positive control vancomycin showed an IC_(50)value of 1.563μg/mL.展开更多
基金Project supported by the National Natural Science Foundation of China(No.32160108)the Science and Technology Special Fund of Hainan Province(No.ZDYF2024SHFZ116)+2 种基金the Specific Research Fund of the Innovation Center for Academicians of Hainan Province(No.YSPTZX202309)the Scientific Research Project of Hainan Higher Education Institutions(No.Hnky2022ZD-6)the Hainan Normal University National College Student Innovation Training Program(No.202411658006)。
文摘new heterocyclic dipeptide with a highly functionalized 1,2-oxazadecaline core,named trichodermamide H(1),and three known analogues,along with three known polyketides,were isolated from the fermentation extract of the mangrovederived fungus Penicillium janthinellum XLN32122.The structure of 1 was elucidated on the basis of extensive 1D and 2D NMR spectra data analysis,HR-ESI-MS,electronic circular dichroism(ECD)calculations.Trichodermamide B(3)exhibited better inhibitory effect on nitric oxide(NO)production in lipopolysaccharide(LPS)induced RAW 264.7 cells with an IC_(50)value of(13.13±0.005)μmol/L than that of the positive control dexamethasone[IC_(50)=(136.84±1.33)μmol/L].Compound 3 exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus(MRSA)with an IC_(50)value of 12.5μg/mL,while the positive control vancomycin showed an IC_(50)value of 1.563μg/mL.
基金Project supported by the National Natural Science Foundation of China(Nos.21462015,31760093)the National Natural Science Foundation of Hainan Province(No.218MS045)the Program for Innovative Research Team in University(No.IRT-16R19)~~
