Migratory birds undertake regular seasonal movements between breeding and non-breeding grounds each year,often spanning intercontinental distances.Several migratory waterbirds are declining globally,owing to multiple ...Migratory birds undertake regular seasonal movements between breeding and non-breeding grounds each year,often spanning intercontinental distances.Several migratory waterbirds are declining globally,owing to multiple threats from parts of annual migration.Therefore,understanding the spatial distribution and conservation challenges of migratory waterbirds is critical for effective flyway-scale conservation.In this study,we compiled a comprehensive dataset of 199 migratory waterbird species,mapped seasonal distribution,and identified population trends and threats along the East Asian-Australasian Flyway and Central Asian Flyway.Species richness exhibited latitudinal gradients along the two flyways,peaking at approximately 60°N in the breeding season and15°N in the non-breeding season.Migration distance and geographic spread significantly varied among orders.More than half of the waterbird species(50.75%)showed decreasing population trends;however,no significant difference in the proportion of population decline was noticed across flyways or orders.A total of 31 species(15.58%)were listed as threatened,most of which were concentrated in the East Asian-Australasian Flyway and primarily affected by the use of biological resources,pollution,and agricultural expansion.Overall,this study provides a comparative assessment of migratory waterbirds in Asian flyways.Our findings highlight the importance of multinational conservation efforts targeting key breeding and non-breeding regions,specifically for threatened species,and underscore the need for coordinated strategies to mitigate multiple,overlapping threats across flyways.These further emphasize that conservation actions should prioritize transboundary habitat networks and policy integration among flyway countries to enhance the long-term resilience of migratory waterbird populations.展开更多
Microorganisms are crucial in the bioremediation of organophosphorus pesticides. However, most functional microorganisms (> 99%) are yet to be cultivated. This study applied two cultivation-independent approaches, ...Microorganisms are crucial in the bioremediation of organophosphorus pesticides. However, most functional microorganisms (> 99%) are yet to be cultivated. This study applied two cultivation-independent approaches, DNA-SIP and magnetic-nanoparticle mediated isolation (MMI), to identify the functional microorganisms in degrading dimethoate in agricultural soils. MMI identified five dimethoate degraders: Pseudomonas, Bacillus, Ramlibacter, Arthrobacter, and Rhodococcus, whereas DNA-SIP identified three dimethoate degraders: Ramlibacter, Arthrobacter, and Rhodococcus. Also, MMI showed higher resolution than DNA-SIP in identifying functional microorganisms. Two organic phosphohydrolase (OPH) genes: ophC2 and ophB, were involved in dimethoate metabolism, as revealed by DNA-SIP and MMI. The degradation products of dimethoate include omethoate, O,O,S-trimethyl thiophosphorothioate, N-methyl-2-sulfanylacetamide, O,O-diethyl S-hydrogen phosphorodithioate, O,O,O-trimethyl thiophosphate, O,O,S-trimethyl thiophosphorodithioate, and O,O,O-trimethyl phosphoric. This study emphasizes the feasibility of using SIP and MMI to explore the functional dimethoate degraders, expanding our knowledge of microbial resources with cultivation-independent approaches.展开更多
基金supported by The National Key Research and Development Program of China(2022YFC3204304)Jiangsu Provincial Innovation Research Program on Carbon Peaking and Carbon Neutrality(BT2024012)Postgraduate Research&Practice Innovation Program of Jiangsu Province。
文摘Migratory birds undertake regular seasonal movements between breeding and non-breeding grounds each year,often spanning intercontinental distances.Several migratory waterbirds are declining globally,owing to multiple threats from parts of annual migration.Therefore,understanding the spatial distribution and conservation challenges of migratory waterbirds is critical for effective flyway-scale conservation.In this study,we compiled a comprehensive dataset of 199 migratory waterbird species,mapped seasonal distribution,and identified population trends and threats along the East Asian-Australasian Flyway and Central Asian Flyway.Species richness exhibited latitudinal gradients along the two flyways,peaking at approximately 60°N in the breeding season and15°N in the non-breeding season.Migration distance and geographic spread significantly varied among orders.More than half of the waterbird species(50.75%)showed decreasing population trends;however,no significant difference in the proportion of population decline was noticed across flyways or orders.A total of 31 species(15.58%)were listed as threatened,most of which were concentrated in the East Asian-Australasian Flyway and primarily affected by the use of biological resources,pollution,and agricultural expansion.Overall,this study provides a comparative assessment of migratory waterbirds in Asian flyways.Our findings highlight the importance of multinational conservation efforts targeting key breeding and non-breeding regions,specifically for threatened species,and underscore the need for coordinated strategies to mitigate multiple,overlapping threats across flyways.These further emphasize that conservation actions should prioritize transboundary habitat networks and policy integration among flyway countries to enhance the long-term resilience of migratory waterbird populations.
基金The authors would like to thank the National Natural Science Foundation of China(Nos.42177359 and 41807119)the Natural Science Foundation of Beijing(No.8212030)+1 种基金the Fundamental Research Funds for the Central Universities(Nos.FRF-TP-20-010A3 and FRF-IDRY-22-001)the Open Fund of National Engineering Laboratory for Site Remediation Technologies(No.NEL-SRT201907).
文摘Microorganisms are crucial in the bioremediation of organophosphorus pesticides. However, most functional microorganisms (> 99%) are yet to be cultivated. This study applied two cultivation-independent approaches, DNA-SIP and magnetic-nanoparticle mediated isolation (MMI), to identify the functional microorganisms in degrading dimethoate in agricultural soils. MMI identified five dimethoate degraders: Pseudomonas, Bacillus, Ramlibacter, Arthrobacter, and Rhodococcus, whereas DNA-SIP identified three dimethoate degraders: Ramlibacter, Arthrobacter, and Rhodococcus. Also, MMI showed higher resolution than DNA-SIP in identifying functional microorganisms. Two organic phosphohydrolase (OPH) genes: ophC2 and ophB, were involved in dimethoate metabolism, as revealed by DNA-SIP and MMI. The degradation products of dimethoate include omethoate, O,O,S-trimethyl thiophosphorothioate, N-methyl-2-sulfanylacetamide, O,O-diethyl S-hydrogen phosphorodithioate, O,O,O-trimethyl thiophosphate, O,O,S-trimethyl thiophosphorodithioate, and O,O,O-trimethyl phosphoric. This study emphasizes the feasibility of using SIP and MMI to explore the functional dimethoate degraders, expanding our knowledge of microbial resources with cultivation-independent approaches.
