The aim of this experiment was to investigate the effect of Lycium barbarum polysaccharides(LBP) on alleviating soybean meal-induced enteritis(SBMIE) in spotted sea bass Lateolabrax maculatus. The diet with 44% fishme...The aim of this experiment was to investigate the effect of Lycium barbarum polysaccharides(LBP) on alleviating soybean meal-induced enteritis(SBMIE) in spotted sea bass Lateolabrax maculatus. The diet with 44% fishmeal(FM) content was used as a blank control, and soybean meal(SM) was used to replace50% FM as an experimental control to induce enteritis. Then, on the basis of experimental control, 0.10%0.15%, and 0.20% LBP were added as experimental diets. A total of 225 spotted sea bass(44.52 ± 0.24 g)were randomly divided into 5 groups and fed the corresponding diets for 52 d. The results showed that0.15% LBP decreased serum D-lactic acid(D-LA) content and diamine oxidase(DAO) activity(P < 0.05). In addition, in all LBP supplementation groups, the intestinal tissue morphology was significantly improved(P < 0.05);the intestinal microbial structure gradually recovered to a level close to that without adding SM;and the microbial species richness and diversity were significantly increased(P < 0.05). Through transcriptomic and metabolomic analysis, it was found that the expression of proinflammatory factors such as interleukin-1β(IL-1β), interleukin-12(IL-12), nuclear factor kappa B subunit 2(NF-κB2), and Tolllike receptor 2(TLR2) were significantly down-regulated in the mitogen-activated protein kinase(MAPK)and Toll-like receptor signaling pathways(P < 0.05), and the important tight junction protein gene Occludin was up-regulated(P < 0.05). In addition, LBP down-regulated saponin metabolites and upregulated amino acid metabolites(P < 0.05). In conclusion, LBP demonstrated a significant alleviating effect on SBMIE of spotted sea bass L. maculatus.展开更多
基金funded by Science and Technology Planning Project in Fujian,China(grant No.2015N0010)Science and Technology Planning Project in Xiamen,China(grant No.3502Z20143017).
文摘The aim of this experiment was to investigate the effect of Lycium barbarum polysaccharides(LBP) on alleviating soybean meal-induced enteritis(SBMIE) in spotted sea bass Lateolabrax maculatus. The diet with 44% fishmeal(FM) content was used as a blank control, and soybean meal(SM) was used to replace50% FM as an experimental control to induce enteritis. Then, on the basis of experimental control, 0.10%0.15%, and 0.20% LBP were added as experimental diets. A total of 225 spotted sea bass(44.52 ± 0.24 g)were randomly divided into 5 groups and fed the corresponding diets for 52 d. The results showed that0.15% LBP decreased serum D-lactic acid(D-LA) content and diamine oxidase(DAO) activity(P < 0.05). In addition, in all LBP supplementation groups, the intestinal tissue morphology was significantly improved(P < 0.05);the intestinal microbial structure gradually recovered to a level close to that without adding SM;and the microbial species richness and diversity were significantly increased(P < 0.05). Through transcriptomic and metabolomic analysis, it was found that the expression of proinflammatory factors such as interleukin-1β(IL-1β), interleukin-12(IL-12), nuclear factor kappa B subunit 2(NF-κB2), and Tolllike receptor 2(TLR2) were significantly down-regulated in the mitogen-activated protein kinase(MAPK)and Toll-like receptor signaling pathways(P < 0.05), and the important tight junction protein gene Occludin was up-regulated(P < 0.05). In addition, LBP down-regulated saponin metabolites and upregulated amino acid metabolites(P < 0.05). In conclusion, LBP demonstrated a significant alleviating effect on SBMIE of spotted sea bass L. maculatus.
