Vitiligo is an immune memory skin disease.T-cell factor 1(TCF1)is essential for maintaining the memory Tcell pool.There is an urgent need to investigate the characteristics of peripheral memory T-cell profile and TCF1...Vitiligo is an immune memory skin disease.T-cell factor 1(TCF1)is essential for maintaining the memory Tcell pool.There is an urgent need to investigate the characteristics of peripheral memory T-cell profile and TCF1^(+)T-cell frequencies in patients with vitiligo.In this study,31 patients with active vitiligo(AV),22 with stable vitiligo(SV),and 30 healthy controls(HCs)were included.We measured circulating memory and TCF1^(+)T-cell frequencies using flow cytometry.The Spearman's rank test was used to evaluate the correlation between cell frequencies and disease characteristics.Receiver operating characteristic curves(ROC)were constructed to investigate the discriminative power of the cell subpopulations.Circulating CD4^(+)and CD8^(+)terminally differentiated effector memory T-cell(TeMRA)frequencies were significantly higher in the AV group than in HCs(P<0.05).TCF1^(+)T-cellsubpopulations were widespread increased in patients with vitiligo(P<0.05).After adjusting for potential confounders,CD8^(+)and CD4^(+)central memory(Tcm)cells,and CD8^(+)TeMRA were correlated with disease activity(P<0.05).The combined diagnostic value of the four(naive,effector memory,TcM,and TeMRA)CD8^(+)TCF1^(+)T-cell subsets was relatively high(area under the ROC curve(AUC)=0.804,sensitivity=71.70%,specificity=83.34%),and the CD8^(+)T-cell subsets combination performed well in discriminating disease activity(AUC=0.849,sensitivity=70.97%,specificity=90.91%).We demonstrated an altered circulating memory T-cell profile and increased TCF1^(+)T-cell percentage in patients with vitiligo.T-cell subpopulations had a strong value for vitiligo diagnosis and activity evaluation.This evidence presents a potential new pharmacological target for inhibiting autoimmunity that leads to vitiligo.展开更多
基金funded by the National Natural Science Foundation of China(Grant Nos.:82222059,82173416,and 81930087).
文摘Vitiligo is an immune memory skin disease.T-cell factor 1(TCF1)is essential for maintaining the memory Tcell pool.There is an urgent need to investigate the characteristics of peripheral memory T-cell profile and TCF1^(+)T-cell frequencies in patients with vitiligo.In this study,31 patients with active vitiligo(AV),22 with stable vitiligo(SV),and 30 healthy controls(HCs)were included.We measured circulating memory and TCF1^(+)T-cell frequencies using flow cytometry.The Spearman's rank test was used to evaluate the correlation between cell frequencies and disease characteristics.Receiver operating characteristic curves(ROC)were constructed to investigate the discriminative power of the cell subpopulations.Circulating CD4^(+)and CD8^(+)terminally differentiated effector memory T-cell(TeMRA)frequencies were significantly higher in the AV group than in HCs(P<0.05).TCF1^(+)T-cellsubpopulations were widespread increased in patients with vitiligo(P<0.05).After adjusting for potential confounders,CD8^(+)and CD4^(+)central memory(Tcm)cells,and CD8^(+)TeMRA were correlated with disease activity(P<0.05).The combined diagnostic value of the four(naive,effector memory,TcM,and TeMRA)CD8^(+)TCF1^(+)T-cell subsets was relatively high(area under the ROC curve(AUC)=0.804,sensitivity=71.70%,specificity=83.34%),and the CD8^(+)T-cell subsets combination performed well in discriminating disease activity(AUC=0.849,sensitivity=70.97%,specificity=90.91%).We demonstrated an altered circulating memory T-cell profile and increased TCF1^(+)T-cell percentage in patients with vitiligo.T-cell subpopulations had a strong value for vitiligo diagnosis and activity evaluation.This evidence presents a potential new pharmacological target for inhibiting autoimmunity that leads to vitiligo.
