Cell culture media is a significant contributor to the high cost of bioprocesses.This study explored the potential of algae to re-condition spent cell culture medium,which may reduce the costs of pharmaceutical and la...Cell culture media is a significant contributor to the high cost of bioprocesses.This study explored the potential of algae to re-condition spent cell culture medium,which may reduce the costs of pharmaceutical and lab-grown-meat manufacturing.Chlorella sorokiniana,a thermally resistant microalgal species,exhibited heterotrophic and mixotrophic growth in mammalian cell culture growth media(GM).Spent animal cell media,generated by culturing quail myoblast cells(QM7s)for 4 or 8d(4D-SGM and 8D-SGM,respectively),was harvested for algal culture.Increased algal growth was observed in 4D-SGM,when compared to fresh media after 3d(optical density of 1.39±0.22,0.47±0.17,p≤0.05).Within 72 h,ammonia and glucose were eliminated from 4D-SGM with algal treatment.After treating 4D-SGM with algae,the treated media was reintroduced into QM7 cell cultures.No cytotoxic effects were observed on QM7 cells grown in algal-treated growth media and QM7 cells exhibited better metabolic activity in algal-treated spent medium than in untreated spent medium(80.85±12.02%and 44.57±10.82%activity of fresh media group,respectively,p≤0.05).These results suggest that C.sorokiniana can be grown in spent media at 37℃,sequester ammonia,and potentially extend the lifespan of media,thereby enabling more affordable bioprocesses.展开更多
基金funded via laboratory startup funds to Glenn Gaudette from Boston Collegea New Harvest fellowship awarded to Richard Thyden。
文摘Cell culture media is a significant contributor to the high cost of bioprocesses.This study explored the potential of algae to re-condition spent cell culture medium,which may reduce the costs of pharmaceutical and lab-grown-meat manufacturing.Chlorella sorokiniana,a thermally resistant microalgal species,exhibited heterotrophic and mixotrophic growth in mammalian cell culture growth media(GM).Spent animal cell media,generated by culturing quail myoblast cells(QM7s)for 4 or 8d(4D-SGM and 8D-SGM,respectively),was harvested for algal culture.Increased algal growth was observed in 4D-SGM,when compared to fresh media after 3d(optical density of 1.39±0.22,0.47±0.17,p≤0.05).Within 72 h,ammonia and glucose were eliminated from 4D-SGM with algal treatment.After treating 4D-SGM with algae,the treated media was reintroduced into QM7 cell cultures.No cytotoxic effects were observed on QM7 cells grown in algal-treated growth media and QM7 cells exhibited better metabolic activity in algal-treated spent medium than in untreated spent medium(80.85±12.02%and 44.57±10.82%activity of fresh media group,respectively,p≤0.05).These results suggest that C.sorokiniana can be grown in spent media at 37℃,sequester ammonia,and potentially extend the lifespan of media,thereby enabling more affordable bioprocesses.
