Wheat grain morphology,particularly grain length(GL)and width(GW),is a key determinant of yield.To improve the suboptimal grain dimensions of the local anthocyanin-rich variety Guizi 1(GZ1),we crossed it with Zhongyan...Wheat grain morphology,particularly grain length(GL)and width(GW),is a key determinant of yield.To improve the suboptimal grain dimensions of the local anthocyanin-rich variety Guizi 1(GZ1),we crossed it with Zhongyan 96-3(ZY96-3),an elite germplasm known for faster grain filling and superior grain size.A genotyping-by-sequencing(GBS)approach was applied to an F_(2)population of 110 individuals derived from GZ1×ZY96-3,resulting in the identification of 23,134 high-quality SNPs.Most of the SNPs associated with GL and GW were clustered on chromosomes 2B,3A,and 3B.QTL mapping for GL revealed two major loci,GL1 on chromosome 2B and GL2 on chromosome 3B,and eight candidate genes were identified within their corresponding intervals(2B:63.6–70.4 Mb;3B:631.5–633.3 Mb).These genes encode proteins potentially involved in grain size regulation,including a TOR2 regulation-associated protein,erect spike 2(EP2),fibroblast growth factor 6(FGF6),cellulose synthase-like(CSLD),RelA/pot homologue three family protein,and three GDSL esterase/lipase(GLIP)proteins.Additionally,we detected a QTL associated with GW on chromosome 3A and identified two candidate genes,TOR2 regulation and starch synthase within the 61.4–68.5 Mb interval.Overall,this study provides a strong theoretical and technical basis for wheat genetic improvement and offers valuable resources for precise QTL mapping and candidate gene discovery.展开更多
Seed priming is an effective seed pretreatment technology that enhances germination and overall crop performance by optimizing seed hydration and metabolic processes before planting.Seed quality is a critical determin...Seed priming is an effective seed pretreatment technology that enhances germination and overall crop performance by optimizing seed hydration and metabolic processes before planting.Seed quality is a critical determinant of cotton(Gossypium hirsutum)crop performance,influencing germination,plant vigor,and yield.This study evaluates the effects of seed priming with potassium salts(1%and 2%KCl and K2SO4)on germination,morphological traits,and Cry1Ac gene expression in three Bt cotton cultivars(IUB-2013,NIAB-878B,FH-142)as Cry1Ac enhance the pest resistance in Bt cotton and reduce the plant’s dependence on chemical insecticides.Seeds were primed for six hours,air-dried,and sown in the field.Germination rates,plant height,number of bolls per plant,boll weight,seed cotton yield,and ginning outturn(GOT)were assessed at crop maturity.Cry1Ac gene expression was quantified to explore the influence of priming treatments on transgene activity.Results demonstrated that 1%K2SO4 priming significantly enhanced germination and yield-related traits,with Cry1Ac expression peaking in the IUB-2013 cultivar under 1%K2SO4 treatment.These findings suggest that potassium-based halopriming improves cotton seedling establishment and Bt gene expression.This study addresses the critical gaps in understanding the effects of seed halopriming on morphological traits,germination,and expression of the Cry1Ac gene in Bt cotton while providing a novel eco-friendly and cost-effective halopriming approach,offering the potential to improve cotton production.展开更多
基金Funding for this project was provided by the National Natural Science Foundation of China(Grants No.32160456,32360474,32360486,32260496)the Key Laboratory of Functional Agriculture of Guizhou Provincial Higher Education Institutions(Grant No.Qianjiaoji(2023)007).
文摘Wheat grain morphology,particularly grain length(GL)and width(GW),is a key determinant of yield.To improve the suboptimal grain dimensions of the local anthocyanin-rich variety Guizi 1(GZ1),we crossed it with Zhongyan 96-3(ZY96-3),an elite germplasm known for faster grain filling and superior grain size.A genotyping-by-sequencing(GBS)approach was applied to an F_(2)population of 110 individuals derived from GZ1×ZY96-3,resulting in the identification of 23,134 high-quality SNPs.Most of the SNPs associated with GL and GW were clustered on chromosomes 2B,3A,and 3B.QTL mapping for GL revealed two major loci,GL1 on chromosome 2B and GL2 on chromosome 3B,and eight candidate genes were identified within their corresponding intervals(2B:63.6–70.4 Mb;3B:631.5–633.3 Mb).These genes encode proteins potentially involved in grain size regulation,including a TOR2 regulation-associated protein,erect spike 2(EP2),fibroblast growth factor 6(FGF6),cellulose synthase-like(CSLD),RelA/pot homologue three family protein,and three GDSL esterase/lipase(GLIP)proteins.Additionally,we detected a QTL associated with GW on chromosome 3A and identified two candidate genes,TOR2 regulation and starch synthase within the 61.4–68.5 Mb interval.Overall,this study provides a strong theoretical and technical basis for wheat genetic improvement and offers valuable resources for precise QTL mapping and candidate gene discovery.
基金National Natural Science Foundation of China(3216045632360474+2 种基金32360486)grants from the Provincial Basic Research Program(Natural Science)([2020]1Z018)Provincial Key Technology R&D Program([2021]YiBan272).
文摘Seed priming is an effective seed pretreatment technology that enhances germination and overall crop performance by optimizing seed hydration and metabolic processes before planting.Seed quality is a critical determinant of cotton(Gossypium hirsutum)crop performance,influencing germination,plant vigor,and yield.This study evaluates the effects of seed priming with potassium salts(1%and 2%KCl and K2SO4)on germination,morphological traits,and Cry1Ac gene expression in three Bt cotton cultivars(IUB-2013,NIAB-878B,FH-142)as Cry1Ac enhance the pest resistance in Bt cotton and reduce the plant’s dependence on chemical insecticides.Seeds were primed for six hours,air-dried,and sown in the field.Germination rates,plant height,number of bolls per plant,boll weight,seed cotton yield,and ginning outturn(GOT)were assessed at crop maturity.Cry1Ac gene expression was quantified to explore the influence of priming treatments on transgene activity.Results demonstrated that 1%K2SO4 priming significantly enhanced germination and yield-related traits,with Cry1Ac expression peaking in the IUB-2013 cultivar under 1%K2SO4 treatment.These findings suggest that potassium-based halopriming improves cotton seedling establishment and Bt gene expression.This study addresses the critical gaps in understanding the effects of seed halopriming on morphological traits,germination,and expression of the Cry1Ac gene in Bt cotton while providing a novel eco-friendly and cost-effective halopriming approach,offering the potential to improve cotton production.
