The phytohormone auxin plays crucial roles in nearly every aspect of plant growth and development.Auxin signaling is activated through the phytohormone-induced proteasomal degradation of the Auxin/INDOLE-3-ACETIC ACID...The phytohormone auxin plays crucial roles in nearly every aspect of plant growth and development.Auxin signaling is activated through the phytohormone-induced proteasomal degradation of the Auxin/INDOLE-3-ACETIC ACID(Aux/IAA)family of transcriptional repressors.Notably,many auxin-modulated physiological processes are also regulated by nitric oxide(NO)that executes its biological effects predominantly through protein S-nitrosylation at specific cysteine residues.However,little is known about the molecular mechanisms in regulating the interactive NO and auxin networks.Here,we show that NO represses auxin signaling by inhibiting IAA17 protein degradation.NO induces the S-nitrosylation of Cys-70 located in the intrinsically disordered region of IAA17,which inhibits the TIR1-IAA17 interaction and consequently the proteasomal degradation of IAA17.The accumulation of a higher level of IAA17 attenuates auxin response.Moreover,an IAA17^(C70W)nitrosomimetic mutation renders the accumulation of a higher level of the mutated protein,thereby causing partial resistance to auxin and defective lateral root development.Taken together,these results suggest that S-nitrosylation of IAA17 at Cys-70 inhibits its interaction with TIR1,thereby negatively regulating auxin signaling.This study provides unique molecular insights into the redox-based auxin signaling in regulating plant growth and development.展开更多
Cellular function relies on numerous diverse processes,all happening simultaneously.One means by which cells manage these processes is through the use of biomolecular condensates.These membrane-less compartments,often...Cellular function relies on numerous diverse processes,all happening simultaneously.One means by which cells manage these processes is through the use of biomolecular condensates.These membrane-less compartments,often created through phase separation,influence cellular mechanisms ranging from reaction kinetics to biomolecule sequestration(Banani et al.,2017).In plants,biomolecular condensates play roles in desiccation tolerance(Belott et al.,2020),transcriptional control(Powers et al.,2019;Bondos et al.,2021),and a myriad of other diverse processes(Emenecker et al.,2021).Recently,condensates have been discovered to have the ability to exert forces through interactions with cellular components.For example,capillary interaction between condensates and other cellular components can generate force;however,more study is necessary to elucidate these mechanisms and interactions(Kusumaatmaja et al.,2021;Gouveia et al.,2022).A recent work by Wang et al.(2024)has revealed new insights into how biomolecular condensates may exert forces on membranes through bending and scission.Further,they provide in silico methods and a model to investigate the forces at play during membrane wetting.展开更多
Plants are exposed to a range of daily and seasonal temperatures and thus are required to regulate their cellular processes under fluctuating temperature conditions.Among various temperature-responsive genes,GLYCINE-R...Plants are exposed to a range of daily and seasonal temperatures and thus are required to regulate their cellular processes under fluctuating temperature conditions.Among various temperature-responsive genes,GLYCINE-RICH RNA BINDING PROTEIN7(GRP7)encodes a glycine-rich RNA-binding protein that provides freezing tolerance to plants(Carpenter et al.,1994;Kim et al.,2008)by acting as an RNA chaperone(Kwak et al.,2011).展开更多
基金supported by grants from the National Natural Science Foundation of China (31830017)Chinese Academy of Sciences (XDB27030207)+1 种基金the Hainan Excellent Talent TeamState Key Laboratory of Plant Genomics (SKLPG2023-22)
文摘The phytohormone auxin plays crucial roles in nearly every aspect of plant growth and development.Auxin signaling is activated through the phytohormone-induced proteasomal degradation of the Auxin/INDOLE-3-ACETIC ACID(Aux/IAA)family of transcriptional repressors.Notably,many auxin-modulated physiological processes are also regulated by nitric oxide(NO)that executes its biological effects predominantly through protein S-nitrosylation at specific cysteine residues.However,little is known about the molecular mechanisms in regulating the interactive NO and auxin networks.Here,we show that NO represses auxin signaling by inhibiting IAA17 protein degradation.NO induces the S-nitrosylation of Cys-70 located in the intrinsically disordered region of IAA17,which inhibits the TIR1-IAA17 interaction and consequently the proteasomal degradation of IAA17.The accumulation of a higher level of IAA17 attenuates auxin response.Moreover,an IAA17^(C70W)nitrosomimetic mutation renders the accumulation of a higher level of the mutated protein,thereby causing partial resistance to auxin and defective lateral root development.Taken together,these results suggest that S-nitrosylation of IAA17 at Cys-70 inhibits its interaction with TIR1,thereby negatively regulating auxin signaling.This study provides unique molecular insights into the redox-based auxin signaling in regulating plant growth and development.
基金supported by the National Science Foundation(PGRP BIO-2112056)the National Institutes of Health(R35 GM136338)Duke Beyond the Horizons funds.
文摘Cellular function relies on numerous diverse processes,all happening simultaneously.One means by which cells manage these processes is through the use of biomolecular condensates.These membrane-less compartments,often created through phase separation,influence cellular mechanisms ranging from reaction kinetics to biomolecule sequestration(Banani et al.,2017).In plants,biomolecular condensates play roles in desiccation tolerance(Belott et al.,2020),transcriptional control(Powers et al.,2019;Bondos et al.,2021),and a myriad of other diverse processes(Emenecker et al.,2021).Recently,condensates have been discovered to have the ability to exert forces through interactions with cellular components.For example,capillary interaction between condensates and other cellular components can generate force;however,more study is necessary to elucidate these mechanisms and interactions(Kusumaatmaja et al.,2021;Gouveia et al.,2022).A recent work by Wang et al.(2024)has revealed new insights into how biomolecular condensates may exert forces on membranes through bending and scission.Further,they provide in silico methods and a model to investigate the forces at play during membrane wetting.
基金supported by the National Science Foundation(PGRP BIO-2112056 to L.C.S.)the National Institute of Health(R35 GM136338 to L.C.S.).
文摘Plants are exposed to a range of daily and seasonal temperatures and thus are required to regulate their cellular processes under fluctuating temperature conditions.Among various temperature-responsive genes,GLYCINE-RICH RNA BINDING PROTEIN7(GRP7)encodes a glycine-rich RNA-binding protein that provides freezing tolerance to plants(Carpenter et al.,1994;Kim et al.,2008)by acting as an RNA chaperone(Kwak et al.,2011).
