背景:氧化应激作为体内氧化与抗氧化系统失衡的状态,在骨质疏松症发病过程中扮演着重要角色,然而,目前关于氧化应激与骨质疏松症领域的研究现状及发展趋势尚缺乏系统性分析。目的:采用文献计量学方法分析氧化应激与骨质疏松症领域的研...背景:氧化应激作为体内氧化与抗氧化系统失衡的状态,在骨质疏松症发病过程中扮演着重要角色,然而,目前关于氧化应激与骨质疏松症领域的研究现状及发展趋势尚缺乏系统性分析。目的:采用文献计量学方法分析氧化应激与骨质疏松症领域的研究现状、热点及趋势。方法:通过Web of Science核心数据库,以“oxidative stress”和“osteoporosis”为检索词,限定语言类型为“English”,文献类型为“article”和“review article”,检索时限为1999-01-01/2024-12-31。按照纳入和排除标准筛选文献后,利用CiteSpace(6.3.R1)和VOSviewer(1.6.20)软件对发文量、国家、机构、作者、期刊及关键词等进行数据分析和可视化图谱生成。结果与结论:①发文量分析:共检索到文献2558篇,最终纳入2416篇。1999-2024年,氧化应激与骨质疏松症领域发文量呈显著增长趋势,尤其在2011年后增长迅速,反映出该领域研究的逐步深入和热度提升;②发文国家分析:中国以1088篇的发文量位居第一,但平均每篇被引次数较低;美国以353篇发文量排名第二,但平均每篇被引次数高达74.62次,展现出研究质量的国际影响力;③发文机构分析:中国机构在发文量上占据主导地位,但国际合作水平有待提升,其中上海交通大学和苏州大学等机构合作广泛,被引次数较高;④发文作者和作者共被引分析:少数核心作者如Almeida和Manolagas在该领域影响力显著,研究成果被引次数极高,合作模式多样,以国际作者为主导;⑤发文期刊分析:美国期刊如《Journal of Bone and Mineral Research》在骨质疏松症氧化应激领域的研究中占据核心地位,学术权威性和影响力显著;⑥关键词分析:研究核心聚焦于“骨质疏松”与“氧化应激”的相互作用机制,高频关键词包括“活性氧”“炎症”“成骨细胞”和“破骨细胞”等,关键词聚类分析显示,研究热点集中在炎症、氧化应激与骨代谢失衡、人群与临床研究、抗氧化治疗的开发与应用等方面;⑦氧化应激与骨质疏松症领域研究呈快速增长趋势,中国虽占据主导地位但国际影响力有待提升,未来需深入解析氧化应激信号网络,探索跨疾病交互作用,开发新型抗氧化剂和治疗方法,推动精准医学和多组学技术应用,并加强国际合作与交流,以期为骨质疏松症的预防和治疗提供更为科学和有效的解决方案。展开更多
Although the dynamic properties of subgrade soils in seasonally frozen areas have already been studied, few researchers have considered the influence of shallow groundwater during the freeze–thaw(F–T) cycles. So a m...Although the dynamic properties of subgrade soils in seasonally frozen areas have already been studied, few researchers have considered the influence of shallow groundwater during the freeze–thaw(F–T) cycles. So a multifunctional F–T cycle system was developed to imitate the groundwater recharge in the subgrade during the freezing process and a large number of dynamic triaxial experiments were conducted after the F–T cycles. Some significant factors including the F–T cycle number, compaction degree, confining pressure, cyclic deviator stress, loading frequency, and water content were investigated for the resilient modulus of soils. The experimental results indicated that the dynamic resilient modulus of the subgrade was negatively correlated with the cyclic deviator stress, F–T cycle number, and initial water content, whereas the degree of compaction, confining pressure, and loading frequency could enhance the resilient modulus. Furthermore, a modified model considering the F–T cycle number and stress state was established to predict the dynamic resilient modulus. The calculated results of this modified model were very close to the experimental results. Consequently, calculation of the resilient modulus for F–T cycles considering the dynamic load was appropriate. This study provides reference for research focusing on F–T cycles with groundwater supply and the dynamic resilient moduli of subgrade soils in seasonally frozen areas.展开更多
A new high strength 2A97 Al-Cu-Li-X alloy was subjected to triple-aging of retrogression and re-aging treatments （RRA）. Transmission electron microscopy （TEM）, differential scanning calorimetry （DSC）, and tensil...A new high strength 2A97 Al-Cu-Li-X alloy was subjected to triple-aging of retrogression and re-aging treatments （RRA）. Transmission electron microscopy （TEM）, differential scanning calorimetry （DSC）, and tensile tests were used to investigate the effects of RRA treatment on the microstructures and properties. DSC test reveals the reversion temperature range of the strengthening δ＇ （Al3Li） phase. The results show that the microstructure consists of δ＇ （Al3Li） phase, T1 （Al2CuLi） phase and θ″/θ′（Al2Cu） phase for 2A97 alloy treated by a triple-aging of a retrogression and re-aging treatment in the following order： （1） at 165℃×30 min, （2） at 220 ℃ or 240℃ × 15 min, （3） at 165℃×24 h. The plastic deformation, incorporated into the treatment after secondary high temperature aging, promotes the T1 precipitation during final re-aging. The tensile properties of the alloy treated by the retrogression and re-aging treatment reach the peak level of alloy single-aged at 165℃ in T6 temper.展开更多
BACKGROUND Calpain-2 is a Ca^2+-dependent cysteine protease,and high calpain-2 activity can enhance apoptosis mediated by multiple triggers.AIM To investigate whether calpain-2 can modulate aberrant endoplasmic reticu...BACKGROUND Calpain-2 is a Ca^2+-dependent cysteine protease,and high calpain-2 activity can enhance apoptosis mediated by multiple triggers.AIM To investigate whether calpain-2 can modulate aberrant endoplasmic reticulum(ER)stress-related apoptosis in rat hepatocyte BRL-3A cells.METHODS BRL-3A cells were treated with varying doses of dithiothreitol(DTT),and their viability and apoptosis were quantified by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2-H-tetrazolium bromide and flow cytometry.The expression of ER stress-and apoptosis-related proteins was detected by Western blot analysis.The protease activity of calpain-2 was determined using a fluorescent substrate,Nsuccinyl-Leu-Leu-Val-Tyr-AMC.Intracellular Ca^2+content,and ER and calpain-2 co-localization were characterized by fluorescent microscopy.The impact of calpain-2 silencing by specific small interfering RNA on caspase-12 activation and apoptosis of BRL-3A cells was quantified.RESULTS DTT exhibited dose-dependent cytotoxicity against BRL-3A cells and treatment with 2 mmol/L DTT triggered BRL-3A cell apoptosis.DTT treatment significantly upregulated 78 kDa glucose-regulated protein,activating transcription factor 4,C/EBP-homologous protein expression by>2-fold,and enhanced PRKR-like ER kinase phosphorylation,caspase-12 and caspase-3 cleavage in BRL-3A cells in a trend of time-dependence.DTT treatment also significantly increased intracellular Ca^2+content,calpain-2 expression,and activity by>2-fold in BRL-3A cells.Furthermore,immunofluorescence revealed that DTT treatment promoted the ER accumulation of calpain-2.Moreover,calpain-2 silencing to decrease calpain-2 expression by 85%significantly mitigated DTT-enhanced calpain-2 expression,caspase-12 cleavage,and apoptosis in BRL-3A cells.CONCLUSION The data indicated that Ca^2+-dependent calpain-2 activity promoted the aberrant ER stress-related apoptosis of rat hepatocytes by activating caspase-12 in the ER.展开更多
Given the rapid changes in social structure(urbanization),economic structure(industrialization),and demographic structure(population aging)in China,cancer has become a major public health problem1.Extensive evidence h...Given the rapid changes in social structure(urbanization),economic structure(industrialization),and demographic structure(population aging)in China,cancer has become a major public health problem1.Extensive evidence has indicated that screening can decrease cancer mortality,particularly among high-risk groups,and several representative national and regional cancer screening programs have been launched in China to cope with the increasing burden of cancer.展开更多
BACKGROUND Hepatocellular carcinoma(HCC) is one of the most common malignant tumors with high mortality-to-incidence ratios. Nuclear factor erythroid 2-like 3(NFE2 L3), also known as NRF3, is a member of the cap ‘n&#...BACKGROUND Hepatocellular carcinoma(HCC) is one of the most common malignant tumors with high mortality-to-incidence ratios. Nuclear factor erythroid 2-like 3(NFE2 L3), also known as NRF3, is a member of the cap ‘n' collar basic-region leucine zipper family of transcription factors. NFE2 L3 is involved in the regulation of various biological processes, whereas its role in HCC has not been elucidated.AIM To explore the expression and biological function of NFE2 L3 in HCC.METHODS We analyzed the expression of NFE2 L3 in HCC tissues and its correlation with clinicopathological parameters based on The Cancer Genome Atlas(TCGA) data portal. Short hairpin RNA(shRNA) interference technology was utilized to knock down NFE2 L3 in vitro. Cell apoptosis, clone formation, proliferation, migration,and invasion assays were used to identify the biological effects of NFE2 L3 in BEL-7404 and SMMC-7721 cells. The expression of epithelial-mesenchymal transition(EMT) markers was examined by Western blot analysis.RESULTS TCGA analysis showed that NFE2 L3 expression was significantly positively correlated with tumor grade, T stage, and pathologic stage. The qPCR and Western blot results showed that both the mRNA and protein levels of NFE2 L3 were significantly decreased after shRNA-mediated knockdown in BEL-7404 and SMMC-7721 cells. The shRNA-mediated knockdown of NFE2 L3 could induce apoptosis and inhibit the clone formation and cell proliferation of SMMC-7721 and BEL-7404 cells. NFE2 L3 knockdown also significantly suppressed the migration, invasion, and EMT of the two cell lines.CONCLUSION Our study showed that shRNA-mediated knockdown of NFE2 L3 exhibited tumor-suppressing effects in HCC cells.展开更多
The safe and efficient disposal and utilization of sludge are major issues to be solved in solid waste treatment and environmental protection due to the complex characteristics of sludge and the low rate of innocuous ...The safe and efficient disposal and utilization of sludge are major issues to be solved in solid waste treatment and environmental protection due to the complex characteristics of sludge and the low rate of innocuous treatments.Composting is a process of decomposing organic matter and transformed low-molecular organic acids into high-molecular humus substances under the action of microorganisms.Although land-use after composting has become an important direction for sludge treatment,heavy metal pollution is still the bottleneck problem restrict-ing land use of sludge compost.Adding zeolite,hydroxyapatite,and other conditioning agents to the composting process affects the concentration or form of some heavy metals and effectively reduces the environmental risk.Lime and phosphorus modifiers change heavy metal speciation in samples,playing a role in decreasing biological availability and mobility.In this study,the effects of sludge composting treatment and conditioning agents on the concentrations and forms of heavy metals are reviewed.This review will provide a theoretical basis to treat heavy metals in sludge composting and lay the foundation for the land utilization and waste recycling of sludge.展开更多
Absolute one-handed chiral quinoline tetramers andoctamers containing different oxazolylanilines at the C-terminus have been synthesized.The absolute one-handed sense and diastereomeric excess values were valued by^1H...Absolute one-handed chiral quinoline tetramers andoctamers containing different oxazolylanilines at the C-terminus have been synthesized.The absolute one-handed sense and diastereomeric excess values were valued by^1H NMR.X-ray crystal diffractionand CD studies reveal that the S-oxazolylaniline always induces a P-handed helicity and the absolute helicity is driven by the stable three-center hydrogen bonding between protons in the amide and N atoms in oxazolylaniline and adjacent quinoline ring.CPL investigations demonstrated that S-CQn-a-d are CPL active and its g(lum)values are dependent on its length.Interestingly,the sizes of the substituents in the chiral centers are different,howeve r,they exert no effect on the dissymmetric factors g(abs)and g(lum)of quinoline oligoamide foldamers.展开更多
文摘背景:氧化应激作为体内氧化与抗氧化系统失衡的状态,在骨质疏松症发病过程中扮演着重要角色,然而,目前关于氧化应激与骨质疏松症领域的研究现状及发展趋势尚缺乏系统性分析。目的:采用文献计量学方法分析氧化应激与骨质疏松症领域的研究现状、热点及趋势。方法:通过Web of Science核心数据库,以“oxidative stress”和“osteoporosis”为检索词,限定语言类型为“English”,文献类型为“article”和“review article”,检索时限为1999-01-01/2024-12-31。按照纳入和排除标准筛选文献后,利用CiteSpace(6.3.R1)和VOSviewer(1.6.20)软件对发文量、国家、机构、作者、期刊及关键词等进行数据分析和可视化图谱生成。结果与结论:①发文量分析:共检索到文献2558篇,最终纳入2416篇。1999-2024年,氧化应激与骨质疏松症领域发文量呈显著增长趋势,尤其在2011年后增长迅速,反映出该领域研究的逐步深入和热度提升;②发文国家分析:中国以1088篇的发文量位居第一,但平均每篇被引次数较低;美国以353篇发文量排名第二,但平均每篇被引次数高达74.62次,展现出研究质量的国际影响力;③发文机构分析:中国机构在发文量上占据主导地位,但国际合作水平有待提升,其中上海交通大学和苏州大学等机构合作广泛,被引次数较高;④发文作者和作者共被引分析:少数核心作者如Almeida和Manolagas在该领域影响力显著,研究成果被引次数极高,合作模式多样,以国际作者为主导;⑤发文期刊分析:美国期刊如《Journal of Bone and Mineral Research》在骨质疏松症氧化应激领域的研究中占据核心地位,学术权威性和影响力显著;⑥关键词分析:研究核心聚焦于“骨质疏松”与“氧化应激”的相互作用机制,高频关键词包括“活性氧”“炎症”“成骨细胞”和“破骨细胞”等,关键词聚类分析显示,研究热点集中在炎症、氧化应激与骨代谢失衡、人群与临床研究、抗氧化治疗的开发与应用等方面;⑦氧化应激与骨质疏松症领域研究呈快速增长趋势,中国虽占据主导地位但国际影响力有待提升,未来需深入解析氧化应激信号网络,探索跨疾病交互作用,开发新型抗氧化剂和治疗方法,推动精准医学和多组学技术应用,并加强国际合作与交流,以期为骨质疏松症的预防和治疗提供更为科学和有效的解决方案。
基金Projects(41672312, 41972294) supported by the National Natural Science Foundation of ChinaProject(2017CFA056) supported by the Outstanding Youth Foundation of Hubei Province, ChinaProject(KFJ170104) supported by the Changsha University of Science & Technology via Open Fund of National Engineering Laboratory of Highway Maintenance Technology, China。
文摘Although the dynamic properties of subgrade soils in seasonally frozen areas have already been studied, few researchers have considered the influence of shallow groundwater during the freeze–thaw(F–T) cycles. So a multifunctional F–T cycle system was developed to imitate the groundwater recharge in the subgrade during the freezing process and a large number of dynamic triaxial experiments were conducted after the F–T cycles. Some significant factors including the F–T cycle number, compaction degree, confining pressure, cyclic deviator stress, loading frequency, and water content were investigated for the resilient modulus of soils. The experimental results indicated that the dynamic resilient modulus of the subgrade was negatively correlated with the cyclic deviator stress, F–T cycle number, and initial water content, whereas the degree of compaction, confining pressure, and loading frequency could enhance the resilient modulus. Furthermore, a modified model considering the F–T cycle number and stress state was established to predict the dynamic resilient modulus. The calculated results of this modified model were very close to the experimental results. Consequently, calculation of the resilient modulus for F–T cycles considering the dynamic load was appropriate. This study provides reference for research focusing on F–T cycles with groundwater supply and the dynamic resilient moduli of subgrade soils in seasonally frozen areas.
基金"973" Key Project of Chinese National Programs for Fundamental Research and Development (2005CB623705)
文摘A new high strength 2A97 Al-Cu-Li-X alloy was subjected to triple-aging of retrogression and re-aging treatments （RRA）. Transmission electron microscopy （TEM）, differential scanning calorimetry （DSC）, and tensile tests were used to investigate the effects of RRA treatment on the microstructures and properties. DSC test reveals the reversion temperature range of the strengthening δ＇ （Al3Li） phase. The results show that the microstructure consists of δ＇ （Al3Li） phase, T1 （Al2CuLi） phase and θ″/θ′（Al2Cu） phase for 2A97 alloy treated by a triple-aging of a retrogression and re-aging treatment in the following order： （1） at 165℃×30 min, （2） at 220 ℃ or 240℃ × 15 min, （3） at 165℃×24 h. The plastic deformation, incorporated into the treatment after secondary high temperature aging, promotes the T1 precipitation during final re-aging. The tensile properties of the alloy treated by the retrogression and re-aging treatment reach the peak level of alloy single-aged at 165℃ in T6 temper.
基金the National Natural Science Foundation of China,No.81560105the Department of Science and Technology of Guizhou Province,No.LH(2014)7074。
文摘BACKGROUND Calpain-2 is a Ca^2+-dependent cysteine protease,and high calpain-2 activity can enhance apoptosis mediated by multiple triggers.AIM To investigate whether calpain-2 can modulate aberrant endoplasmic reticulum(ER)stress-related apoptosis in rat hepatocyte BRL-3A cells.METHODS BRL-3A cells were treated with varying doses of dithiothreitol(DTT),and their viability and apoptosis were quantified by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2-H-tetrazolium bromide and flow cytometry.The expression of ER stress-and apoptosis-related proteins was detected by Western blot analysis.The protease activity of calpain-2 was determined using a fluorescent substrate,Nsuccinyl-Leu-Leu-Val-Tyr-AMC.Intracellular Ca^2+content,and ER and calpain-2 co-localization were characterized by fluorescent microscopy.The impact of calpain-2 silencing by specific small interfering RNA on caspase-12 activation and apoptosis of BRL-3A cells was quantified.RESULTS DTT exhibited dose-dependent cytotoxicity against BRL-3A cells and treatment with 2 mmol/L DTT triggered BRL-3A cell apoptosis.DTT treatment significantly upregulated 78 kDa glucose-regulated protein,activating transcription factor 4,C/EBP-homologous protein expression by>2-fold,and enhanced PRKR-like ER kinase phosphorylation,caspase-12 and caspase-3 cleavage in BRL-3A cells in a trend of time-dependence.DTT treatment also significantly increased intracellular Ca^2+content,calpain-2 expression,and activity by>2-fold in BRL-3A cells.Furthermore,immunofluorescence revealed that DTT treatment promoted the ER accumulation of calpain-2.Moreover,calpain-2 silencing to decrease calpain-2 expression by 85%significantly mitigated DTT-enhanced calpain-2 expression,caspase-12 cleavage,and apoptosis in BRL-3A cells.CONCLUSION The data indicated that Ca^2+-dependent calpain-2 activity promoted the aberrant ER stress-related apoptosis of rat hepatocytes by activating caspase-12 in the ER.
基金supported by grants from the National Key Research and Development Program of China(Grant No.2021YFC2500400)the National Natural Science Foundation of China(Grant Nos.81974439&82204121)+2 种基金the Beijing-Tianjin-Hebei Basic Research Cooperation Special Project(20JCZXJC00090)the Tianjin Health Committee Foundation(Grant No.TJWJ2021MS008)the Tianjin Key Medical Discipline(Specialty)Construction Project(Grant No.TJYXZDXK-009A).
文摘Given the rapid changes in social structure(urbanization),economic structure(industrialization),and demographic structure(population aging)in China,cancer has become a major public health problem1.Extensive evidence has indicated that screening can decrease cancer mortality,particularly among high-risk groups,and several representative national and regional cancer screening programs have been launched in China to cope with the increasing burden of cancer.
基金the Changzhou High-Level Medical Talents Training Project,No.2016ZCLJ002
文摘BACKGROUND Hepatocellular carcinoma(HCC) is one of the most common malignant tumors with high mortality-to-incidence ratios. Nuclear factor erythroid 2-like 3(NFE2 L3), also known as NRF3, is a member of the cap ‘n' collar basic-region leucine zipper family of transcription factors. NFE2 L3 is involved in the regulation of various biological processes, whereas its role in HCC has not been elucidated.AIM To explore the expression and biological function of NFE2 L3 in HCC.METHODS We analyzed the expression of NFE2 L3 in HCC tissues and its correlation with clinicopathological parameters based on The Cancer Genome Atlas(TCGA) data portal. Short hairpin RNA(shRNA) interference technology was utilized to knock down NFE2 L3 in vitro. Cell apoptosis, clone formation, proliferation, migration,and invasion assays were used to identify the biological effects of NFE2 L3 in BEL-7404 and SMMC-7721 cells. The expression of epithelial-mesenchymal transition(EMT) markers was examined by Western blot analysis.RESULTS TCGA analysis showed that NFE2 L3 expression was significantly positively correlated with tumor grade, T stage, and pathologic stage. The qPCR and Western blot results showed that both the mRNA and protein levels of NFE2 L3 were significantly decreased after shRNA-mediated knockdown in BEL-7404 and SMMC-7721 cells. The shRNA-mediated knockdown of NFE2 L3 could induce apoptosis and inhibit the clone formation and cell proliferation of SMMC-7721 and BEL-7404 cells. NFE2 L3 knockdown also significantly suppressed the migration, invasion, and EMT of the two cell lines.CONCLUSION Our study showed that shRNA-mediated knockdown of NFE2 L3 exhibited tumor-suppressing effects in HCC cells.
基金The study was financially supported by the National Natural Science Foundation of China(41371464)the Study Abroad Fund of Yangtze University,the Study Fund of Engineering Research of Ecology and Agricultural Use of Wetland,the Ministry of Education,Huangshi city Project for Institute Development(HL20181201)+1 种基金the Technical Innovation Project(Major Project)of Hubei Province,China(2016ACA176)the Scientific Research Project of Hubei Polytechnic University(16xjz04R).
文摘The safe and efficient disposal and utilization of sludge are major issues to be solved in solid waste treatment and environmental protection due to the complex characteristics of sludge and the low rate of innocuous treatments.Composting is a process of decomposing organic matter and transformed low-molecular organic acids into high-molecular humus substances under the action of microorganisms.Although land-use after composting has become an important direction for sludge treatment,heavy metal pollution is still the bottleneck problem restrict-ing land use of sludge compost.Adding zeolite,hydroxyapatite,and other conditioning agents to the composting process affects the concentration or form of some heavy metals and effectively reduces the environmental risk.Lime and phosphorus modifiers change heavy metal speciation in samples,playing a role in decreasing biological availability and mobility.In this study,the effects of sludge composting treatment and conditioning agents on the concentrations and forms of heavy metals are reviewed.This review will provide a theoretical basis to treat heavy metals in sludge composting and lay the foundation for the land utilization and waste recycling of sludge.
基金financial supports from the 973 Program(No.2015CB856502)the National Natural Science Foundation of China(No.21672026)。
文摘Absolute one-handed chiral quinoline tetramers andoctamers containing different oxazolylanilines at the C-terminus have been synthesized.The absolute one-handed sense and diastereomeric excess values were valued by^1H NMR.X-ray crystal diffractionand CD studies reveal that the S-oxazolylaniline always induces a P-handed helicity and the absolute helicity is driven by the stable three-center hydrogen bonding between protons in the amide and N atoms in oxazolylaniline and adjacent quinoline ring.CPL investigations demonstrated that S-CQn-a-d are CPL active and its g(lum)values are dependent on its length.Interestingly,the sizes of the substituents in the chiral centers are different,howeve r,they exert no effect on the dissymmetric factors g(abs)and g(lum)of quinoline oligoamide foldamers.
