The careful observation and evaluation of the squamocolumnar junction(SCJ)are essential requirements in colposcopy procedures.While traditional colposcopes with visible light offer high resolution and sensitivity,the ...The careful observation and evaluation of the squamocolumnar junction(SCJ)are essential requirements in colposcopy procedures.While traditional colposcopes with visible light offer high resolution and sensitivity,the presence of glare patterns on the cervix limits the visualization of deeper tissue features.Cross-polarization imaging,on the other hand,overcomes this limitation by effectively removing glare and providing greater optical penetration depth to noninvasively examine subsurface tissue structures.In this study,we present the potential of polarized light in enhancing diagnostics through cross-polarization imaging for the identification and contrast enhancement of squamous and columnar epithelium(CE)in cases of cervical ectropion.Our results demonstrate that polarized light colposcopy offers valuable diagnostic information that complements traditional colposcopy.By effectively reducing glare and improving visibility during cervical examinations,polarized light colposcopy proves to be a useful tool.Furthermore,we introduce a novel method that practically enhances the contrast ratio(CR)between columnar and squamous epithelium(SE)in colposcopic images.This method significantly increases the contrast between these tissue types,facilitating clearer differentiation and improving diagnostic accuracy.Notably,the combination of the cross-polarization imaging technique with our proposed algorithm enables the clear observation of the SCJ boundary.These findings emphasize the potential of our approach in enhancing the accuracy and effectiveness of polarized colposcopy for evaluating cervical tissue.展开更多
Collagen provides tissue strength and structural integrity.Quanti fication of the orientated dispersion of collagen fibers is an important factor when studying the mechanical properties of the cervix.In this study,for...Collagen provides tissue strength and structural integrity.Quanti fication of the orientated dispersion of collagen fibers is an important factor when studying the mechanical properties of the cervix.In this study,for the first time,a new method for rapid characterization of the collagen fiber orientations of the cervix using linearly polarized light colposcopy is presented.A total of 24 colposcopic images were captured using a cross-polarized imaging system with white LED light sources.In the preprocessing stage,the Red channel of the RGB image was chosen,which contains no information of the blood vessels because of the low-absorption of blood cells in the red region.OrientationJ,which is an ImageJ plug-in,was used to estimate the local orientation of the collagen fibers.The result shows that in the nonpregnant cervix,the middle zone(Zone 2)has circumferentially aligned collagen fibers while the inner zone(Zone 1)has randomly arranged.The collagen fiber dispersion in Zone 2 is much smaller than that in Zone 1 at all four quadrants region(anterior,posterior,left,and right quadrant).This new analysis technique could potentially combine with diagnostic tools to provide a quantitative platform of collagen fibers in the clinic.展开更多
Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology...Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology approach, we exploited the responsiveness of Arabidopsis UDP-glucosyltransferase-encoding UGT74E2 towards mitochondrial per- turbation in order to look for novel mechanisms regulating mitochondria-to-nucleus communication. The most potent inducers of UGT74E2 shared a （2-furyl）acrylate （FAA） substructure that negatively affected mitochondrial function and was identified before as an auxin transcriptional inhibitor. Based on these premises, we demonstrated that perturbed mitochondria negatively affect the auxin signaling machinery. Moreover, chemical perturbation of polar auxin transport and auxin biosynthesis was sufficient to induce mitochondrial retrograde markers and their transcript abundance was constitutively elevated in the absence of the auxin transcriptional activators ARF7 and ARF19.展开更多
文摘The careful observation and evaluation of the squamocolumnar junction(SCJ)are essential requirements in colposcopy procedures.While traditional colposcopes with visible light offer high resolution and sensitivity,the presence of glare patterns on the cervix limits the visualization of deeper tissue features.Cross-polarization imaging,on the other hand,overcomes this limitation by effectively removing glare and providing greater optical penetration depth to noninvasively examine subsurface tissue structures.In this study,we present the potential of polarized light in enhancing diagnostics through cross-polarization imaging for the identification and contrast enhancement of squamous and columnar epithelium(CE)in cases of cervical ectropion.Our results demonstrate that polarized light colposcopy offers valuable diagnostic information that complements traditional colposcopy.By effectively reducing glare and improving visibility during cervical examinations,polarized light colposcopy proves to be a useful tool.Furthermore,we introduce a novel method that practically enhances the contrast ratio(CR)between columnar and squamous epithelium(SE)in colposcopic images.This method significantly increases the contrast between these tissue types,facilitating clearer differentiation and improving diagnostic accuracy.Notably,the combination of the cross-polarization imaging technique with our proposed algorithm enables the clear observation of the SCJ boundary.These findings emphasize the potential of our approach in enhancing the accuracy and effectiveness of polarized colposcopy for evaluating cervical tissue.
基金supported by the Ho Chi Minh City Department of Science and Technology of Vietnam,under grant number 116/2020/HDQPTKHCN.
文摘Collagen provides tissue strength and structural integrity.Quanti fication of the orientated dispersion of collagen fibers is an important factor when studying the mechanical properties of the cervix.In this study,for the first time,a new method for rapid characterization of the collagen fiber orientations of the cervix using linearly polarized light colposcopy is presented.A total of 24 colposcopic images were captured using a cross-polarized imaging system with white LED light sources.In the preprocessing stage,the Red channel of the RGB image was chosen,which contains no information of the blood vessels because of the low-absorption of blood cells in the red region.OrientationJ,which is an ImageJ plug-in,was used to estimate the local orientation of the collagen fibers.The result shows that in the nonpregnant cervix,the middle zone(Zone 2)has circumferentially aligned collagen fibers while the inner zone(Zone 1)has randomly arranged.The collagen fiber dispersion in Zone 2 is much smaller than that in Zone 1 at all four quadrants region(anterior,posterior,left,and right quadrant).This new analysis technique could potentially combine with diagnostic tools to provide a quantitative platform of collagen fibers in the clinic.
文摘Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology approach, we exploited the responsiveness of Arabidopsis UDP-glucosyltransferase-encoding UGT74E2 towards mitochondrial per- turbation in order to look for novel mechanisms regulating mitochondria-to-nucleus communication. The most potent inducers of UGT74E2 shared a （2-furyl）acrylate （FAA） substructure that negatively affected mitochondrial function and was identified before as an auxin transcriptional inhibitor. Based on these premises, we demonstrated that perturbed mitochondria negatively affect the auxin signaling machinery. Moreover, chemical perturbation of polar auxin transport and auxin biosynthesis was sufficient to induce mitochondrial retrograde markers and their transcript abundance was constitutively elevated in the absence of the auxin transcriptional activators ARF7 and ARF19.
