The Metal Stable Isotope Geochemistry Laboratory(MSIGL)at the University of Science and Technology of China has developed state-of-the-art analytical methods for twelve stable isotope systems,including Mg,Si,V,Fe,Cu,Z...The Metal Stable Isotope Geochemistry Laboratory(MSIGL)at the University of Science and Technology of China has developed state-of-the-art analytical methods for twelve stable isotope systems,including Mg,Si,V,Fe,Cu,Zn,Rb,Sr,Ag,Cd,Ba,and U.Geological and biological samples were first digested by acid dissolution or alkali dissolution.The target element was subsequently purified by the column chromatography method.A Neptune Plus MC-ICP-MS was used to measure isotope compositions and the isotope bias caused during measurements was calibrated by standard bracketing and/or the double spike method.The analytical procedure was carefully checked to ensure the high precision and accuracy of the data.Here,we summarized the protocol of these established methods and compiled the standard data measured at our lab as well as those reported in literature.This comprehensive dataset can serve as a reliable benchmark for calibration,method validation,and quality assurance in metal stable isotope analyses.展开更多
Pollination methods play an important role in developing F1 populations.Our study presents an improved method of petal-assisted artificial pollination in tea plants.The field operation using this method exhibited high...Pollination methods play an important role in developing F1 populations.Our study presents an improved method of petal-assisted artificial pollination in tea plants.The field operation using this method exhibited high efficiency in which more than 500 pistillate flowers per day can be pollinated per capita,and the pollen amount from only one staminate flower was sufficient to pollinate more than five pistillate flowers.Additionally,we surveyed the fruit-setting ratio in three F1 groups,which were established using this method and the influence of rainfall on the fruit-setting ratio.The results indicate that the fruit-setting ratio was superior among the tested F1 population with an average value of 12.90%,and a significant negative correlation was observed between rainfall and fruit-setting ratio.By analyzing the growth states of each F1 population,both a high germination rate and survival rate were observed.Furthermore,the purity for 184 individuals in the F1 population of'Jiaming 1'×'Longjing 43'(J×L)were analyzed with 32 selected simple sequence repeats(SSRs).The purposes of this paper are(1)to demonstrate that this method is beneficial to establish high purity F1 populations and(2)to identify the number of SSR markers that are suitable for identification.The parentage analysis detected nine false-hybrid individuals,and the rate of false-hybrid was only 4.89%at 95%confidence,demonstrating that a low false-hybrid rate can be achieved using the method.Furthermore,one to two SSR markers randomly selected from each linkage group or chromosome were recommended as a sufficient quantity for SSR identification.展开更多
基金the National Science Foundation of China(Nos.42273007 and 42473008)the Distinguished Young Scholars of Anhui,China(No.2408085J021)。
文摘The Metal Stable Isotope Geochemistry Laboratory(MSIGL)at the University of Science and Technology of China has developed state-of-the-art analytical methods for twelve stable isotope systems,including Mg,Si,V,Fe,Cu,Zn,Rb,Sr,Ag,Cd,Ba,and U.Geological and biological samples were first digested by acid dissolution or alkali dissolution.The target element was subsequently purified by the column chromatography method.A Neptune Plus MC-ICP-MS was used to measure isotope compositions and the isotope bias caused during measurements was calibrated by standard bracketing and/or the double spike method.The analytical procedure was carefully checked to ensure the high precision and accuracy of the data.Here,we summarized the protocol of these established methods and compiled the standard data measured at our lab as well as those reported in literature.This comprehensive dataset can serve as a reliable benchmark for calibration,method validation,and quality assurance in metal stable isotope analyses.
基金supported by the Chinese Modern Agricultural Industrial Technological System(CARS-19)the Key Scientific and Technological Project for New Variety of Tea Plant Breeding of Zhejiang Province(2016C02053).
文摘Pollination methods play an important role in developing F1 populations.Our study presents an improved method of petal-assisted artificial pollination in tea plants.The field operation using this method exhibited high efficiency in which more than 500 pistillate flowers per day can be pollinated per capita,and the pollen amount from only one staminate flower was sufficient to pollinate more than five pistillate flowers.Additionally,we surveyed the fruit-setting ratio in three F1 groups,which were established using this method and the influence of rainfall on the fruit-setting ratio.The results indicate that the fruit-setting ratio was superior among the tested F1 population with an average value of 12.90%,and a significant negative correlation was observed between rainfall and fruit-setting ratio.By analyzing the growth states of each F1 population,both a high germination rate and survival rate were observed.Furthermore,the purity for 184 individuals in the F1 population of'Jiaming 1'×'Longjing 43'(J×L)were analyzed with 32 selected simple sequence repeats(SSRs).The purposes of this paper are(1)to demonstrate that this method is beneficial to establish high purity F1 populations and(2)to identify the number of SSR markers that are suitable for identification.The parentage analysis detected nine false-hybrid individuals,and the rate of false-hybrid was only 4.89%at 95%confidence,demonstrating that a low false-hybrid rate can be achieved using the method.Furthermore,one to two SSR markers randomly selected from each linkage group or chromosome were recommended as a sufficient quantity for SSR identification.
