共同项目负责人(Multiple-Principal Investigator,M-PI)制度是美国国立卫生研究院(National Institutes of Health,NIH)科研项目的重要特征,目前NIH多种研究类项目已采用M-PI模式。M-PI允许在一个项目中设立多个PI,他们在指导项目完成...共同项目负责人(Multiple-Principal Investigator,M-PI)制度是美国国立卫生研究院(National Institutes of Health,NIH)科研项目的重要特征,目前NIH多种研究类项目已采用M-PI模式。M-PI允许在一个项目中设立多个PI,他们在指导项目完成方面具有同等权力和责任。M-PI项目的评审原则与单一PI项目相同,但需要另外提供一份领导方案,说明选择M-PI模式的理由及不同PI之间合作方案。自2006年实施以来,M-PI项目数量持续增长,有效促进了生物医学领域学科交叉和深度融合,其科学性和合理性已得到广泛认可。国家自然科学基金委员会医学科学部2022年在生命与医学板块专项中试点Co-PI(Co-Principal Investigator)制,有效引导临床与基础学科交叉融合。本文通过分析NIH实施M-PI政策的运行情况和取得的成果,为我国促进学科交叉融合、推动原始创新提供借鉴,为我国科学基金管理制度的改革和完善提供参考。展开更多
The initial healing stages of bone fracture is a complex physiological process involving a series of spatially and temporally overlapping events,including pathogen clearance,immunological modulation,and osteogenesis.I...The initial healing stages of bone fracture is a complex physiological process involving a series of spatially and temporally overlapping events,including pathogen clearance,immunological modulation,and osteogenesis.In this study,we have developed a piezoelectric and aligned nanofibrous scaffold composed of ZnO@PCL/PVDF with multiple antibacterial,immunomodulatory,and osteogenic effects using electrospinning technology.This scaffold’s piezoelectric signal output under ultrasound(US)control can be similar to the physiological electrical signals of healthy bone tissue,creating a truly biomimetic electrical microenvironment in the bone defect.In vitro studies have shown that ZnO@PCL/PVDF scaffold significantly enhances the proliferation,migration,and osteogenic differentiation of MC3T3-E1 cells under piezoelectric drive provided by ultrasound.Furthermore,the scaffold exhibits inhibitory effects on the growth of E.coli and S.aureus,as well as the ability to induce M2 macrophage polarization,indicating potent antibacterial and immunomodulatory properties.In vivo experiments demonstrated that the ZnO@PCL/PVDF scaffold can accelerate the repair of mandibular defects in rats,effectively inhibit bacterial colonization,and reduce inflammatory responses.Altogether,this study confirms that the newly developed ZnO@PCL/PVDF scaffold effectively promotes bone repair by truly mimicking the endogenous electrical microenvironment and precisely regulating the temporospatial disorders of initial bone healing,thus providing a simple and effective solution for bone defects.展开更多
基金funded by National Natural Science Foundation of China(Nos.82151312,82272493,and 82072406)the Beijing Science Nova Program(No.20220484155)+1 种基金the Natural Science Foundation of Shaanxi Province(No.2023-YBSF-426)Beijing Jishuitan Hospital Elite Young Scholar Programme(No.XKGG2021).
文摘The initial healing stages of bone fracture is a complex physiological process involving a series of spatially and temporally overlapping events,including pathogen clearance,immunological modulation,and osteogenesis.In this study,we have developed a piezoelectric and aligned nanofibrous scaffold composed of ZnO@PCL/PVDF with multiple antibacterial,immunomodulatory,and osteogenic effects using electrospinning technology.This scaffold’s piezoelectric signal output under ultrasound(US)control can be similar to the physiological electrical signals of healthy bone tissue,creating a truly biomimetic electrical microenvironment in the bone defect.In vitro studies have shown that ZnO@PCL/PVDF scaffold significantly enhances the proliferation,migration,and osteogenic differentiation of MC3T3-E1 cells under piezoelectric drive provided by ultrasound.Furthermore,the scaffold exhibits inhibitory effects on the growth of E.coli and S.aureus,as well as the ability to induce M2 macrophage polarization,indicating potent antibacterial and immunomodulatory properties.In vivo experiments demonstrated that the ZnO@PCL/PVDF scaffold can accelerate the repair of mandibular defects in rats,effectively inhibit bacterial colonization,and reduce inflammatory responses.Altogether,this study confirms that the newly developed ZnO@PCL/PVDF scaffold effectively promotes bone repair by truly mimicking the endogenous electrical microenvironment and precisely regulating the temporospatial disorders of initial bone healing,thus providing a simple and effective solution for bone defects.
