Podophyllotoxin is isolated mainly from the rhizomes of Podophyllum plants, and serves as the main precursor for synthesis of anticancer drugs, such as VP-16 and VM-26. VP-16 and VM-26 are used for curing lung cancer,...Podophyllotoxin is isolated mainly from the rhizomes of Podophyllum plants, and serves as the main precursor for synthesis of anticancer drugs, such as VP-16 and VM-26. VP-16 and VM-26 are used for curing lung cancer, testicular cancer, neuroblastoma, hepatoma and other tumors. However, these plants are all near-extinction species due to over-collection and their own biological characteristics. The chemical synthesis of podophyllotoxin is so complicated that its price is unbelievably high. This paper discusses the current status of the biosynthetic pathway of podophyllotoxin and that of the podophyllotoxin production using several biotechnological approaches such as plant organ cultures, plant cell cultures with both flasks and bioreactors, hairy root cultures, bioconversions and metabolic regulations.展开更多
The putative transgenic hybrid triploid poplars [(P. tomentosa P. bolleana) P. tomentosa] with CpTI gene have been outplanted in test field for 2 years. Although the authors previous studies have proved that they ar...The putative transgenic hybrid triploid poplars [(P. tomentosa P. bolleana) P. tomentosa] with CpTI gene have been outplanted in test field for 2 years. Although the authors previous studies have proved that they are highly resistant to 3 species of poplar-threatening insect pests and contain high content of CpTI protein in foliage, incorporation status of foreign CpTI gene in poplar genome is uncertain. In this present study, the incorporation of foreign CpTI gene in genome of 5 transgenic poplars was confirmed by PCR and Southern blotting analysis. DNA amplification showed that there were clear DNA bands of about 450bp specific to CpTI gene in transgenic lanes, while no corresponding band in non-transgenic lane was observed. Correspondingly, clear DNA hybridization signals and no signal were exhibited on film for DNA Southern blotting analysis in transgenic lanes and non-transgenic lane, respectively, which further confirmed the stable integration of foreign CpTI gene in genome of 2-year-old transgenic poplar.展开更多
基金Supported by the Natural Science Foundation of Tibet (Grant No. 2002-66)
文摘Podophyllotoxin is isolated mainly from the rhizomes of Podophyllum plants, and serves as the main precursor for synthesis of anticancer drugs, such as VP-16 and VM-26. VP-16 and VM-26 are used for curing lung cancer, testicular cancer, neuroblastoma, hepatoma and other tumors. However, these plants are all near-extinction species due to over-collection and their own biological characteristics. The chemical synthesis of podophyllotoxin is so complicated that its price is unbelievably high. This paper discusses the current status of the biosynthetic pathway of podophyllotoxin and that of the podophyllotoxin production using several biotechnological approaches such as plant organ cultures, plant cell cultures with both flasks and bioreactors, hairy root cultures, bioconversions and metabolic regulations.
基金Supported by the National Project in Transgenic Plant and Application (Grant No. J2002-B003)
文摘The putative transgenic hybrid triploid poplars [(P. tomentosa P. bolleana) P. tomentosa] with CpTI gene have been outplanted in test field for 2 years. Although the authors previous studies have proved that they are highly resistant to 3 species of poplar-threatening insect pests and contain high content of CpTI protein in foliage, incorporation status of foreign CpTI gene in poplar genome is uncertain. In this present study, the incorporation of foreign CpTI gene in genome of 5 transgenic poplars was confirmed by PCR and Southern blotting analysis. DNA amplification showed that there were clear DNA bands of about 450bp specific to CpTI gene in transgenic lanes, while no corresponding band in non-transgenic lane was observed. Correspondingly, clear DNA hybridization signals and no signal were exhibited on film for DNA Southern blotting analysis in transgenic lanes and non-transgenic lane, respectively, which further confirmed the stable integration of foreign CpTI gene in genome of 2-year-old transgenic poplar.
