为研究不同苜蓿和燕麦混合比例制备的发酵型全混合日粮(fermented total mixed ration,FTMR)对羔羊的生长性能、血清生化指标及免疫力的影响。试验选择健康状况良好、体况一致(17.09±0.32)kg的小尾寒羊×乌珠穆沁羊杂交一代羔...为研究不同苜蓿和燕麦混合比例制备的发酵型全混合日粮(fermented total mixed ration,FTMR)对羔羊的生长性能、血清生化指标及免疫力的影响。试验选择健康状况良好、体况一致(17.09±0.32)kg的小尾寒羊×乌珠穆沁羊杂交一代羔羊(公羔)25只,按体重接近原则将羔羊随机分为5组,每组5只。饲喂不同苜蓿与燕麦配比制备的FTMR,各试验组日粮中苜蓿与燕麦占比分别为FTMR1(苜蓿:燕麦=29:35)、FTMR2(苜蓿:燕麦=30:30)、FTMR3(苜蓿:燕麦=34:29)、FTMR4(苜蓿:燕麦=35:26)和FTMR5(苜蓿:燕麦=39:25)。预试期7 d,正试期23 d。结果表明:FTMR1组的平均日增重、总增重和干物质采食量显著高于其他处理(P<0.05),FTMR2、FTMR3、FTMR4和FTMR5组总增重和平均日增重无显著差异(P>0.05);FTMR3组体高显著高于FTMR2组(P<0.05)。各组间羊血液常规指标值均处于正常范围,除白蛋白(ALB)和葡萄糖(GLU)含量在各处理组间无显著差异外(P>0.05),苜蓿与燕麦混合比例对各处理组试验羊血液生化指标影响显著(P<0.05)。FTMR2和FTMR5组谷丙转氨酶(ALT)含量显著低于FTMR4组,FTMR5组碱性磷酸酶(ALP)含量显著低于其他处理(P<0.05),FTMR5组三酰甘油(TG)含量显著低于其他处理(P<0.05);FTMR5组免疫力指标显著高于其他处理(P<0.05),其他各组间免疫力指标无显著差异(P>0.05)。综上所述,苜蓿与燕麦混合制备的FTMR对羔羊生长发育、血液生化和免疫力都具有显著影响,FTMR5组饲喂效果最好。展开更多
Background Advances in the understanding of cardiovascular pathogenesis have highlighted that inflammation plays a central role in athemsclemtic coronary heart disease.Therefore,exploring pharmacologically based anti-...Background Advances in the understanding of cardiovascular pathogenesis have highlighted that inflammation plays a central role in athemsclemtic coronary heart disease.Therefore,exploring pharmacologically based anti-inflammatory treatments to be used in cardiovascular therapeutics is worthwhile to promote the discovery of novel ways of treating cardiovascular disorders.Methods The myocardial cell line H9c2（2-1） was exposed to lipopolysacchadde （LPS） in culture and resulted in a cellular pro-inflammation status,miR-21 microRNA levels were detected using quantitative real-time polymerase chain reaction （Q-RT-PCR）.The influence of Iovastatin on miR-21 under normal and pro-inflammatory conditions was tested after being added to the cell culture mixture for 24 hours.Conditional gene function of two predicted cardiovascular system relevant downstream targets of miR-21,protein phosphatase 1 regulatory subunit 3A （PPP1R3A） and signal transducer and activator of transcription 3 （STAT3）,were analyzed with immunoblotting.Results Forty-eight hours of LPS treatment significantly increased the miR-21 to 170.71%±34.32% of control levels （P=0.002）.Co-treatment with Iovastatin for 24 hours before harvesting attenuated the up-regulation of miR-21 （P=0.013）.Twenty-four hours of Iovastatin exposure up-regulated PPP1R3A to 143.85%±21.89% of control levels in cardiomyocytes （P=0.023）.Lovastatin up-regulated the phosphorylation level of STAT3 compared to the background LPS pretreatment （P=0.0077）,this effect was significantly （P=0.018） blunted when miR-21 was functionally inhibited.Conclusions miR-21 plays a major role in the regulation of the cellular anti-inflammation effects of Iovastatin.展开更多
文摘Background Advances in the understanding of cardiovascular pathogenesis have highlighted that inflammation plays a central role in athemsclemtic coronary heart disease.Therefore,exploring pharmacologically based anti-inflammatory treatments to be used in cardiovascular therapeutics is worthwhile to promote the discovery of novel ways of treating cardiovascular disorders.Methods The myocardial cell line H9c2（2-1） was exposed to lipopolysacchadde （LPS） in culture and resulted in a cellular pro-inflammation status,miR-21 microRNA levels were detected using quantitative real-time polymerase chain reaction （Q-RT-PCR）.The influence of Iovastatin on miR-21 under normal and pro-inflammatory conditions was tested after being added to the cell culture mixture for 24 hours.Conditional gene function of two predicted cardiovascular system relevant downstream targets of miR-21,protein phosphatase 1 regulatory subunit 3A （PPP1R3A） and signal transducer and activator of transcription 3 （STAT3）,were analyzed with immunoblotting.Results Forty-eight hours of LPS treatment significantly increased the miR-21 to 170.71%±34.32% of control levels （P=0.002）.Co-treatment with Iovastatin for 24 hours before harvesting attenuated the up-regulation of miR-21 （P=0.013）.Twenty-four hours of Iovastatin exposure up-regulated PPP1R3A to 143.85%±21.89% of control levels in cardiomyocytes （P=0.023）.Lovastatin up-regulated the phosphorylation level of STAT3 compared to the background LPS pretreatment （P=0.0077）,this effect was significantly （P=0.018） blunted when miR-21 was functionally inhibited.Conclusions miR-21 plays a major role in the regulation of the cellular anti-inflammation effects of Iovastatin.
