Schwannoma surgeries pose a significant risk of postoperative neurological impairment.While intraoperative neuromonitoring(IONM)has improved surgical outcomes,it offers an indirect assessment of neural structures and ...Schwannoma surgeries pose a significant risk of postoperative neurological impairment.While intraoperative neuromonitoring(IONM)has improved surgical outcomes,it offers an indirect assessment of neural structures and functions.However,during the surgeries,it is not feasible to achieve comprehensive visualization of the nerves.To address this limitation,we introduced a multi-channel flexible microelectrode array(FMEA)characterized by its exceptional resolution,consistent conductivity,and unwavering electrical properties.FMEA conforms precisely to the uneven tumor surface during IONM,capturing detailed spatiotemporal patterns of neural signals.Consequently,neurosurgeons can delineate nerve trajectories on the schwannoma surface with heightened precision and evaluate the functional potential of the residual nerve by analyzing signal amplitudes.For surgical guidance,we developed algorithms enabling real-time intraoperative neuro-mapping.This innovation is poised to refine schwannoma surgical practices,promoting nerve anatomical preservation after surgery and guaranteeing postoperative neural outcomes.展开更多
The nematode Bursaphelenchus xylophilus is the causal agent of pine wilt disease and is one of the most destructive plant-parasitic nematodes worldwide.As there are no effective control measures,early and accurate det...The nematode Bursaphelenchus xylophilus is the causal agent of pine wilt disease and is one of the most destructive plant-parasitic nematodes worldwide.As there are no effective control measures,early and accurate detection is crucial to prevent disease spread.Here,we developed a droplet digital PCR(ddPCR)assay and a recombinase polymerase amplification-lateral flow assay(RPA-LFA)for detecting B.xylophilus and compared these methods with real-time quantitative PCR(qPCR).The results showed that this ddPCR assay was highly specific for B.xylophilus and could be quantified through the 5S gene copy numbers.Compared to qPCR,ddPCR is more sensitive and achieves absolute quantification.Additionally,the results of the RPA-LFA were 100%consistent with the positive qPCR results but significantly reduced the required reaction time to within 30 min.Together,establishing and combining these two assays can offer an effective method for the early detection of B.xylophilus,i.e.,the ddPCR assay represents a promising alternative method for the precise quantitative detection of B.xylophilus,while the RPA-LFA is a simple,rapid and visual method that can be used for rapid detection of B.xylophilus in the field and in resource-limited conditions.展开更多
基金supported by the Natural Science Foundation of Beijing Municipality(L234023)National Natural Science Foundation of China(82201170,82027805,82402429)National Key Research and Development Program of China(2022YFF1202301).
文摘Schwannoma surgeries pose a significant risk of postoperative neurological impairment.While intraoperative neuromonitoring(IONM)has improved surgical outcomes,it offers an indirect assessment of neural structures and functions.However,during the surgeries,it is not feasible to achieve comprehensive visualization of the nerves.To address this limitation,we introduced a multi-channel flexible microelectrode array(FMEA)characterized by its exceptional resolution,consistent conductivity,and unwavering electrical properties.FMEA conforms precisely to the uneven tumor surface during IONM,capturing detailed spatiotemporal patterns of neural signals.Consequently,neurosurgeons can delineate nerve trajectories on the schwannoma surface with heightened precision and evaluate the functional potential of the residual nerve by analyzing signal amplitudes.For surgical guidance,we developed algorithms enabling real-time intraoperative neuro-mapping.This innovation is poised to refine schwannoma surgical practices,promoting nerve anatomical preservation after surgery and guaranteeing postoperative neural outcomes.
基金supported by the Science and Technology Innovation Program of Xiongan New Area(2023XAGG0065)Fundamental Research Funds for the Central Universities(QNTD202510).
文摘The nematode Bursaphelenchus xylophilus is the causal agent of pine wilt disease and is one of the most destructive plant-parasitic nematodes worldwide.As there are no effective control measures,early and accurate detection is crucial to prevent disease spread.Here,we developed a droplet digital PCR(ddPCR)assay and a recombinase polymerase amplification-lateral flow assay(RPA-LFA)for detecting B.xylophilus and compared these methods with real-time quantitative PCR(qPCR).The results showed that this ddPCR assay was highly specific for B.xylophilus and could be quantified through the 5S gene copy numbers.Compared to qPCR,ddPCR is more sensitive and achieves absolute quantification.Additionally,the results of the RPA-LFA were 100%consistent with the positive qPCR results but significantly reduced the required reaction time to within 30 min.Together,establishing and combining these two assays can offer an effective method for the early detection of B.xylophilus,i.e.,the ddPCR assay represents a promising alternative method for the precise quantitative detection of B.xylophilus,while the RPA-LFA is a simple,rapid and visual method that can be used for rapid detection of B.xylophilus in the field and in resource-limited conditions.
