Amine-responsive fluorescent indicator films for meat freshness were constructed by incorporating β-glucan grafted with fluorescein isothiocyanate(FITC)in polyvinyl alcohol(PVA).The structural,thermal,and responsive ...Amine-responsive fluorescent indicator films for meat freshness were constructed by incorporating β-glucan grafted with fluorescein isothiocyanate(FITC)in polyvinyl alcohol(PVA).The structural,thermal,and responsive properties of grafted copolymers(βGlu-FITC)were investigated.The results of 1H NMR spectra showed that βGlu-FITC exhibited proton signals between 6.5 and 6.7 ppm,suggesting the successful grafting of FITC onto β-glucan.The fluorescence quantum yield of βGlu-FITC was 18.72%,which was higher than that of β-glucan of 12.94% at the optimum excitation wavelength of ultraviolet light of 365 nm.With the incorporation of PVA as a film-forming matrix,βGlu-FITC/PVA films showed high yellow-green fluorescent responsiveness under volatile ammonia and pH solution.When the film was used for meat freshness,the 0.06βGlu-FITC/PVA film provided a noticeable fluorescent indication of pork freshness that corresponded to increases in total volatile basic nitrogen,total colony counts,and drip loss,suggesting its potential for meat packaging and as a freshness indicator.展开更多
基金supported by The Basic Research Funds Project for Universities Directly under the Inner Mongolia Autonomous Region(BR230401)Outstanding Youth Incubation Project of College of Food Science of Inner Mongolia Agricultural University(SPYQ202101)+3 种基金Young Innovative Talent of Grassland Talent Project of Inner Mongolia(2020)Youth Science and Technology Talent Development Project of Inner Mongolia Autonomous Region(Innovation Team)(NMGIRT2310)Science and Technology Program Projects in the College of Food Science and Engineering(YLXKZX-NND-015)Science and Technology Project of Food Science and Engineering College,Inner Mongolia Agricultural University(SPKJ202405).
文摘Amine-responsive fluorescent indicator films for meat freshness were constructed by incorporating β-glucan grafted with fluorescein isothiocyanate(FITC)in polyvinyl alcohol(PVA).The structural,thermal,and responsive properties of grafted copolymers(βGlu-FITC)were investigated.The results of 1H NMR spectra showed that βGlu-FITC exhibited proton signals between 6.5 and 6.7 ppm,suggesting the successful grafting of FITC onto β-glucan.The fluorescence quantum yield of βGlu-FITC was 18.72%,which was higher than that of β-glucan of 12.94% at the optimum excitation wavelength of ultraviolet light of 365 nm.With the incorporation of PVA as a film-forming matrix,βGlu-FITC/PVA films showed high yellow-green fluorescent responsiveness under volatile ammonia and pH solution.When the film was used for meat freshness,the 0.06βGlu-FITC/PVA film provided a noticeable fluorescent indication of pork freshness that corresponded to increases in total volatile basic nitrogen,total colony counts,and drip loss,suggesting its potential for meat packaging and as a freshness indicator.
