Selenium is a crucial trace element that contributes to physiological processes in the body as selenoproteins.Selenoproteins serve as an integral role in the body in controlling the redox state of cells and protecting...Selenium is a crucial trace element that contributes to physiological processes in the body as selenoproteins.Selenoproteins serve as an integral role in the body in controlling the redox state of cells and protecting against damage induced by oxidative stress.This study aimed to investigate the effects and possible mechanism of selenium on selenoproteins expression in EA.hy926 cells induced by oxidized low density lipoprotein(oxLDL).The impact of selenium on the viability of EA.hy926 cells was detected by the methylthiazolyldiphenyltetrazolium bromide(MTT)method,and intracellular reactive oxygen species(ROS)level and mitochondrial membrane potential were assessed by fluorescent probe DCFH-DA and JC-1,respectively.RNA-seq,quantitative real-time polymerase chain reaction(qPCR),and Western blot were used to investigate the selenoprotein expression.Selenoprotein mRNA translation efficiency was analyzed by ribosome profiling(Ribo-Seq)coupled with transcriptomics.Our data showed that selenium supplementation(0.5μmol/L)significantly decreased ROS production,increased mitochondrial inner membrane potential and increased the proliferative activity of EA.hy926 cells induced by oxLDL.Moreover,The protective effects of selenium against oxLDL-induced EA.hy926 cell injury were associated with the upregulation of the expressions of selenoproteins glutathione peroxidase 1(GPX1),glutathione peroxidase 4(GPX4),and thioredoxin reductase 1(TXNRD1).Furthermore,the expressions of selenoproteins GPX1 and GPX4 were hierarchically controlled,but the expressions of selenoproteins TXNRD1 were mainly regulated by oxLDL.Finally,Ribo-Seq coupled with transcriptomics results demonstrated that the expressions of selenoproteins GPX1,GPX4,and TXNRD1 were regulated at the translation process level.These findings suggested that selenium could have preventive effects in oxLDL induced EA.hy926 cell injury by regulating the selenoprotein expression,and the selenoproteins expressions at the translation level in vascular endothelial cells need further study.展开更多
Goji berry(Lycium barbarum L.),a traditional Chinese herbal remedy and functional food,contains polysaccharides(LBP)as its main active constituents.LBP has been shown to improve blood glucose and immune function.Howev...Goji berry(Lycium barbarum L.),a traditional Chinese herbal remedy and functional food,contains polysaccharides(LBP)as its main active constituents.LBP has been shown to improve blood glucose and immune function.However,its effects under ageing conditions,particularly with high-fat diet(HFD)consumption,remain unclear.Herein,we evaluated the effects of water-soluble LBP on glucose and lipid metabolism and on immune function in elderly mice fed a high-fat diet.2-month-old and 17-month-old female C57BL/6 mice were used.The mice were assigned to 5 groups:young,ageing,ageing+0.3%LBP,ageing+HFD,and ageing+HFD+0.3%LBP.The results showed that 0.3%LBP extract treatment significantly reduced fasting blood glucose,insulin,total cholesterol,and low-density lipoprotein cholesterol levels and improved glucose tolerance in HFDfed ageing mice.Histological and immunohistochemical analysis demonstrated that LBP extract ameliorated hepatic lipid accumulation and adipocyte hypertrophy and increased GLUT4 expression in adipocytes.Flow cytometry analysis demonstrated that in HFD-fed ageing mice,LBP extract intervention significantly decreased the proportion of peripheral MDSCs and increased the CD4+/CD8+ratio.Additionally,correlation analysis revealed significant associations among fasting blood glucose,MDSCs proportion,and CD4+/CD8+ratio.These findings highlight the preventive effect of LBP on aging-associated metabolic and immune dysfunction.展开更多
基金supported by grants from the National Natural Science Foundation of China(NSFC,81960588)the Ningxia Natural Science Foundation(2020AAC03146)support from the Ningxia Medical University。
文摘Selenium is a crucial trace element that contributes to physiological processes in the body as selenoproteins.Selenoproteins serve as an integral role in the body in controlling the redox state of cells and protecting against damage induced by oxidative stress.This study aimed to investigate the effects and possible mechanism of selenium on selenoproteins expression in EA.hy926 cells induced by oxidized low density lipoprotein(oxLDL).The impact of selenium on the viability of EA.hy926 cells was detected by the methylthiazolyldiphenyltetrazolium bromide(MTT)method,and intracellular reactive oxygen species(ROS)level and mitochondrial membrane potential were assessed by fluorescent probe DCFH-DA and JC-1,respectively.RNA-seq,quantitative real-time polymerase chain reaction(qPCR),and Western blot were used to investigate the selenoprotein expression.Selenoprotein mRNA translation efficiency was analyzed by ribosome profiling(Ribo-Seq)coupled with transcriptomics.Our data showed that selenium supplementation(0.5μmol/L)significantly decreased ROS production,increased mitochondrial inner membrane potential and increased the proliferative activity of EA.hy926 cells induced by oxLDL.Moreover,The protective effects of selenium against oxLDL-induced EA.hy926 cell injury were associated with the upregulation of the expressions of selenoproteins glutathione peroxidase 1(GPX1),glutathione peroxidase 4(GPX4),and thioredoxin reductase 1(TXNRD1).Furthermore,the expressions of selenoproteins GPX1 and GPX4 were hierarchically controlled,but the expressions of selenoproteins TXNRD1 were mainly regulated by oxLDL.Finally,Ribo-Seq coupled with transcriptomics results demonstrated that the expressions of selenoproteins GPX1,GPX4,and TXNRD1 were regulated at the translation process level.These findings suggested that selenium could have preventive effects in oxLDL induced EA.hy926 cell injury by regulating the selenoprotein expression,and the selenoproteins expressions at the translation level in vascular endothelial cells need further study.
基金funded by the Natural Science Foundation of Ningxia(2023AAC03168)Ningxia Key Research and Development Program(2021BEF02008)+2 种基金Innovation Team Project of Ningxia Hui Autonomous Region(2024CXTDOO7)Ningxia Infrastructure Construction Foundation(2025DPC05014)Special Program for Scientific and Technological Cooperation of the Ningxia Academy of Agricultural and Forestry Sciences(DW202306).
文摘Goji berry(Lycium barbarum L.),a traditional Chinese herbal remedy and functional food,contains polysaccharides(LBP)as its main active constituents.LBP has been shown to improve blood glucose and immune function.However,its effects under ageing conditions,particularly with high-fat diet(HFD)consumption,remain unclear.Herein,we evaluated the effects of water-soluble LBP on glucose and lipid metabolism and on immune function in elderly mice fed a high-fat diet.2-month-old and 17-month-old female C57BL/6 mice were used.The mice were assigned to 5 groups:young,ageing,ageing+0.3%LBP,ageing+HFD,and ageing+HFD+0.3%LBP.The results showed that 0.3%LBP extract treatment significantly reduced fasting blood glucose,insulin,total cholesterol,and low-density lipoprotein cholesterol levels and improved glucose tolerance in HFDfed ageing mice.Histological and immunohistochemical analysis demonstrated that LBP extract ameliorated hepatic lipid accumulation and adipocyte hypertrophy and increased GLUT4 expression in adipocytes.Flow cytometry analysis demonstrated that in HFD-fed ageing mice,LBP extract intervention significantly decreased the proportion of peripheral MDSCs and increased the CD4+/CD8+ratio.Additionally,correlation analysis revealed significant associations among fasting blood glucose,MDSCs proportion,and CD4+/CD8+ratio.These findings highlight the preventive effect of LBP on aging-associated metabolic and immune dysfunction.
