As a potential endocrine-disrupting chemical,bisphenol F(BPF)may cause nonalcoholic fatty liver disease(NAFLD)-like changes,but the mechanisms under its pathogenesis as well as the intervention strategies remain uncle...As a potential endocrine-disrupting chemical,bisphenol F(BPF)may cause nonalcoholic fatty liver disease(NAFLD)-like changes,but the mechanisms under its pathogenesis as well as the intervention strategies remain unclear.Using the electron microscopy technology,along with LipidTOX Deep Red neutral and Bodipy 493/503 staining assays,we observed that BPF treatment elicited a striking accumulation of lipid droplets in HepG2 cells,accompanied by an increased total level of triglycerides.At the molecular level,the lipogenesis-associated mRNAs and proteins,including acetyl-CoA carboxylase,fatty acid synthase,stearoyl-CoA desaturase-1,peroxisome proliferator-activated receptor gamma,and CCAAT-enhancer-binding proteins,increased significantly via the AMP-activated protein kinase(AMPK)-mammalian target of rapamycin(mTOR)signaling regulation in both in vitro and in vivo studies.Furthermore,the immunofluorescence results also showed the robust lipogenesis induced by BPF,evident in its ability to promote the translocation of sterol regulatory element-binding protein-1c from the cytoplasm to the nuclei.To investigate the intervention strategies for BPF-induced NAFLD-like changes,we demonstrated that bellidifolin,isolated and purified from Swertia chirayita,significantly attenuated BPF-induced lipid droplet deposition in HepG2 cells and NAFLD-like changes in mice by blocking the expression of lipogenesis-associated proteins.Therefore,the present study elucidates the mechanisms underlying the BPF-induced lipid accumulation in HepG2 cells,while also highlighting the potential of bellidifolin to mitigate BPF-induced NAFLD-like changes.展开更多
Ruminant livestock provide a rich source of products,such as meat,milk,and wool,and play a critical role in global food security and nutrition.Over the past few decades,genomic studies of ruminant livestock have provi...Ruminant livestock provide a rich source of products,such as meat,milk,and wool,and play a critical role in global food security and nutrition.Over the past few decades,genomic studies of ruminant livestock have provided valuable insights into their domestication and the genetic basis of economically important traits,facilitating the breeding of elite varieties.In this review,we summarize the main advancements for domestic ruminants in reference genome assemblies,population genomics,and the identification of functional genes or variants for phenotypic traits.These traits include meat and carcass quality,reproduction,milk production,feed efficiency,wool and cashmere yield,horn development,tail type,coat color,environmental adaptation,and disease resistance.Functional genomic research is entering a new era with the advancements of graphical pangenomics and telomere-to-telomere(T2T)gap-free genome assembly.These advancements promise to improve our understanding of domestication and the molecular mechanisms underlying economically important traits in ruminant livestock.Finally,we provide new perspectives and future directions for genomic research on ruminant genomes.We suggest how ever-increasing multiomics datasets will facilitate future studies and molecular breeding in livestock,including the potential to uncover novel genetic mechanisms underlying phenotypic traits,to enable more accurate genomic prediction models,and to accelerate genetic improvement programs.展开更多
Fumonisins(FBs)are mycotoxins primarily synthesized by Fusarium moniliformis.Among these,FB1 exhibits not only high toxicity towards humans and animals but also carcinogenic properties.The global prevalence of FB1 con...Fumonisins(FBs)are mycotoxins primarily synthesized by Fusarium moniliformis.Among these,FB1 exhibits not only high toxicity towards humans and animals but also carcinogenic properties.The global prevalence of FB1 contamination in cereals and related products,particularly maize,is alarmingly significant.Consequently,the accurate determination of FB1 levels in cereals holds immense importance.In this study,highly sensitive monoclonal antibodies specifically targeting FB1 were prepared and utilized for the establishment of a time-resolved fluorescence immunochromatographic assay(TRFIC)to detect FB1.The parameters of antibody labeling with time-resolved fluorescent microspheres were optimized.The detection time was significantly reduced to 6 min.The limits of detection(LOD)for corn,rice,and feed were determined as 0.496-0.844μg/kg,and the quantification(LOQ)was 0.788-1.322μg/kg.In addition,an indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)was successfully developed.Under optimized conditions,the half inhibitory concentration(IC50)value for FB1 was determined as 2.137μg/L.A strong correlation between the results obtained from these two methods and HPLC-MS/MS analysis was observed in the same samples tested.In conclusion,both immunological methods developed in this work are highly suitable for rapid FB1 detection in real field samples.展开更多
Dexamethasone,a long-acting glucocorticoid,exhibits considerable structural similarity to other glucocorticoids.To avoid false-positive results in immunoassays due to cross-reactivity of antibodies with structural ana...Dexamethasone,a long-acting glucocorticoid,exhibits considerable structural similarity to other glucocorticoids.To avoid false-positive results in immunoassays due to cross-reactivity of antibodies with structural analogues,the preparation of highly specific antibodies is crucial.In this study,a novel hapten DEX-GA was designed and synthesized,and the monoclonal antibody(mAb)3D1 was successfully prepared based on the hapten.The mAb 3D1 has the 50%inhibitory concentration of 0.38 ng/mL for dexamethasone,with cross-reactivity below 7.45%against twelve glucocorticoids including betamethasone,prednisone,triamcinolone and beclomethasone.Based on this antibody,indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)and colloidal gold immunochromatography assay(GICA)were developed for accurate detection of dexamethasone in milk and animal tissues.The limits of detection of ic-ELISA in milk and animal tissues ranged from 0.132 to 0.215μg/kg,which was a 1-3-fold increase in sensitivity compared with the previously reported ic-ELISA method.The GICA method established in this study had a visual limit of detection of 0.3μg/L in milk.Notably,this constitutes the first application of GICA in animal tissues,demonstrating a visual detection limit of 0.2μg/kg.The accuracy and reliability of both ic-ELISA and GICA were validated using liquid chromatography-tandem mass spectrometry.展开更多
Thiamethoxam(TMX)is a widely used second-generation neonicotinoid insecticide.However,improper application could result in the accumulation of residue in edible products,posing risks to consumers.As a result,accuratel...Thiamethoxam(TMX)is a widely used second-generation neonicotinoid insecticide.However,improper application could result in the accumulation of residue in edible products,posing risks to consumers.As a result,accurately identifying TMX residues in foods is essential.In this research,monoclonal antibody(mAb)that specifically recognize TMX was developed.To improve detection sensitivity,a heterologous coating strategy was utilized in TMX detection.Based on the developed monoclonal antibody,ic-ELISA and GICA methods were established.Using the established optimal conditions for ic-ELISA,the IC50 was 0.85μg/L.Additionally,a sample extraction method was developed,which can handle both vegetables and animal-derived food at the same time.The limits of detection(LODs)were 0.099-0.117μg/kg,quantification(LOQs)were 0.143-0.194μg/kg,and the recovery rates were 81.5%-121.3%in lake water,pork,beef,eggs,and spinach.Furthermore,we also optimized key parameters of GICA;the VLOD for eggs was 10μg/kg,while for spinach,beef,and pork,it was 5μg/kg.These two detection methods established in this research can more sensitively and accurately detect TMX in various samples during on-site rapid testing.展开更多
基金supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(Grant No.21KJA330002)Natural Science Foundation of Jiangsu Province(Grant No.BK20211252)+3 种基金Young and Middle-aged Academic Leaders of the"Blue Project"in Jiangsu Universities(2022-2)Jiangsu Health and Family Planning Commission Medical Research Program(Grant No.Z2018035)Project of Public Health Research Center of Jiangnan University(Grant No.JUPH201842)Natural Science Foundation of Nanjing University of Chinese Medicine(Grant No.XZR2020021).
文摘As a potential endocrine-disrupting chemical,bisphenol F(BPF)may cause nonalcoholic fatty liver disease(NAFLD)-like changes,but the mechanisms under its pathogenesis as well as the intervention strategies remain unclear.Using the electron microscopy technology,along with LipidTOX Deep Red neutral and Bodipy 493/503 staining assays,we observed that BPF treatment elicited a striking accumulation of lipid droplets in HepG2 cells,accompanied by an increased total level of triglycerides.At the molecular level,the lipogenesis-associated mRNAs and proteins,including acetyl-CoA carboxylase,fatty acid synthase,stearoyl-CoA desaturase-1,peroxisome proliferator-activated receptor gamma,and CCAAT-enhancer-binding proteins,increased significantly via the AMP-activated protein kinase(AMPK)-mammalian target of rapamycin(mTOR)signaling regulation in both in vitro and in vivo studies.Furthermore,the immunofluorescence results also showed the robust lipogenesis induced by BPF,evident in its ability to promote the translocation of sterol regulatory element-binding protein-1c from the cytoplasm to the nuclei.To investigate the intervention strategies for BPF-induced NAFLD-like changes,we demonstrated that bellidifolin,isolated and purified from Swertia chirayita,significantly attenuated BPF-induced lipid droplet deposition in HepG2 cells and NAFLD-like changes in mice by blocking the expression of lipogenesis-associated proteins.Therefore,the present study elucidates the mechanisms underlying the BPF-induced lipid accumulation in HepG2 cells,while also highlighting the potential of bellidifolin to mitigate BPF-induced NAFLD-like changes.
基金supported by the Project of Northern Agriculture and Livestock Husbandry Technology Innovation Center,Chinese Academy of Agricultural Sciences(BFGJ2022002)the National Key Research and Development Program of China(2021YFD1200900,2023YFF1001003,and 2023YFF1000900)+3 种基金Biological Breeding-National Science and Technology Major Project(2023ZD0407106)the National Natural Science Foundation of China(32102511,31661143014,31972527,32320103006,and 32272845)Chinese Universities Scientific Fund(2024TC162)National High Level Hospital Clinical Research Funding(2023-NHLHCRF-YXHZ-TJMS-09)。
文摘Ruminant livestock provide a rich source of products,such as meat,milk,and wool,and play a critical role in global food security and nutrition.Over the past few decades,genomic studies of ruminant livestock have provided valuable insights into their domestication and the genetic basis of economically important traits,facilitating the breeding of elite varieties.In this review,we summarize the main advancements for domestic ruminants in reference genome assemblies,population genomics,and the identification of functional genes or variants for phenotypic traits.These traits include meat and carcass quality,reproduction,milk production,feed efficiency,wool and cashmere yield,horn development,tail type,coat color,environmental adaptation,and disease resistance.Functional genomic research is entering a new era with the advancements of graphical pangenomics and telomere-to-telomere(T2T)gap-free genome assembly.These advancements promise to improve our understanding of domestication and the molecular mechanisms underlying economically important traits in ruminant livestock.Finally,we provide new perspectives and future directions for genomic research on ruminant genomes.We suggest how ever-increasing multiomics datasets will facilitate future studies and molecular breeding in livestock,including the potential to uncover novel genetic mechanisms underlying phenotypic traits,to enable more accurate genomic prediction models,and to accelerate genetic improvement programs.
基金funded by the National Natural Science Foundation of China(32072920 and 32373067)the National Key Research and Development Programs of China(2023YFD1301001)+1 种基金the HZAU-AGIS Cooperation Fund(SZYJY2022024)the Fundamental Research Funds for the Central Universities(2662022DKPY007).
文摘Fumonisins(FBs)are mycotoxins primarily synthesized by Fusarium moniliformis.Among these,FB1 exhibits not only high toxicity towards humans and animals but also carcinogenic properties.The global prevalence of FB1 contamination in cereals and related products,particularly maize,is alarmingly significant.Consequently,the accurate determination of FB1 levels in cereals holds immense importance.In this study,highly sensitive monoclonal antibodies specifically targeting FB1 were prepared and utilized for the establishment of a time-resolved fluorescence immunochromatographic assay(TRFIC)to detect FB1.The parameters of antibody labeling with time-resolved fluorescent microspheres were optimized.The detection time was significantly reduced to 6 min.The limits of detection(LOD)for corn,rice,and feed were determined as 0.496-0.844μg/kg,and the quantification(LOQ)was 0.788-1.322μg/kg.In addition,an indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)was successfully developed.Under optimized conditions,the half inhibitory concentration(IC50)value for FB1 was determined as 2.137μg/L.A strong correlation between the results obtained from these two methods and HPLC-MS/MS analysis was observed in the same samples tested.In conclusion,both immunological methods developed in this work are highly suitable for rapid FB1 detection in real field samples.
基金funded by the National Natural Science Founda-tion of China(32573424)the National Key Research and Development Programs of China(2023YFD1301001,2024YFF1105705)the Hubei Provincial Natural Science Foundation(2024AFB146).
文摘Dexamethasone,a long-acting glucocorticoid,exhibits considerable structural similarity to other glucocorticoids.To avoid false-positive results in immunoassays due to cross-reactivity of antibodies with structural analogues,the preparation of highly specific antibodies is crucial.In this study,a novel hapten DEX-GA was designed and synthesized,and the monoclonal antibody(mAb)3D1 was successfully prepared based on the hapten.The mAb 3D1 has the 50%inhibitory concentration of 0.38 ng/mL for dexamethasone,with cross-reactivity below 7.45%against twelve glucocorticoids including betamethasone,prednisone,triamcinolone and beclomethasone.Based on this antibody,indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)and colloidal gold immunochromatography assay(GICA)were developed for accurate detection of dexamethasone in milk and animal tissues.The limits of detection of ic-ELISA in milk and animal tissues ranged from 0.132 to 0.215μg/kg,which was a 1-3-fold increase in sensitivity compared with the previously reported ic-ELISA method.The GICA method established in this study had a visual limit of detection of 0.3μg/L in milk.Notably,this constitutes the first application of GICA in animal tissues,demonstrating a visual detection limit of 0.2μg/kg.The accuracy and reliability of both ic-ELISA and GICA were validated using liquid chromatography-tandem mass spectrometry.
基金funded by the National Key Research and Development Programs of China(2024YFF1105705,2023YFD1301001)the National Natural Science Foundation of China(32373067).
文摘Thiamethoxam(TMX)is a widely used second-generation neonicotinoid insecticide.However,improper application could result in the accumulation of residue in edible products,posing risks to consumers.As a result,accurately identifying TMX residues in foods is essential.In this research,monoclonal antibody(mAb)that specifically recognize TMX was developed.To improve detection sensitivity,a heterologous coating strategy was utilized in TMX detection.Based on the developed monoclonal antibody,ic-ELISA and GICA methods were established.Using the established optimal conditions for ic-ELISA,the IC50 was 0.85μg/L.Additionally,a sample extraction method was developed,which can handle both vegetables and animal-derived food at the same time.The limits of detection(LODs)were 0.099-0.117μg/kg,quantification(LOQs)were 0.143-0.194μg/kg,and the recovery rates were 81.5%-121.3%in lake water,pork,beef,eggs,and spinach.Furthermore,we also optimized key parameters of GICA;the VLOD for eggs was 10μg/kg,while for spinach,beef,and pork,it was 5μg/kg.These two detection methods established in this research can more sensitively and accurately detect TMX in various samples during on-site rapid testing.
