Endoplasmic reticulum(ER)is an enclosed three-dimensional eukaryotic membrane network composed of flattened sacs.Fusion of homologous membranes to the ER membrane is essential for the maintenance of this network struc...Endoplasmic reticulum(ER)is an enclosed three-dimensional eukaryotic membrane network composed of flattened sacs.Fusion of homologous membranes to the ER membrane is essential for the maintenance of this network structure.In yeast,ER membrane fusion is mediated by Sey1p,whose paralogues function distinctly in different species.In this study,we investigated the biological functions of MoSEY1 in the devastating rice blast fungus Magnaporthe oryzae by functional genomic approach.Compared to wild type,deletion of MoSEY1 considerably decreased the growth and conidia production of M.oryzae.Additionally,the absence of MoSEY1 delayed appressorium formation and invasive hyphae growth.The appressorium function was also impaired inΔMosey1 mutant.Subcellular localization analysis revealed that MoSey1 is localized at the ER.TheΔMosey1 mutant showed augmented sensitivity to ER stress.Additionally,we found that MoSey1 regulated the unfolded protein response,autophagy,and protein secretion in M.oryzae.In conclusion,our study unveiled the involvement of MoSey1 in the development,pathogenesis,and ER functions in M.oryzae.展开更多
基金supported by grants from the Natural Science Foundation of Fujian Province(2022J01129)Fujian Provincial Science and Technology Key Project(2022NZ030014)+2 种基金Central Guidance on Local Science and Technology Development Fund of Fujian Province(2022L3088)State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products(2021DG700024-KF202406)Special Fund for Science and Technology Innovation of Fujian Agriculture and Forestry University(CXZX2019007G).
文摘Endoplasmic reticulum(ER)is an enclosed three-dimensional eukaryotic membrane network composed of flattened sacs.Fusion of homologous membranes to the ER membrane is essential for the maintenance of this network structure.In yeast,ER membrane fusion is mediated by Sey1p,whose paralogues function distinctly in different species.In this study,we investigated the biological functions of MoSEY1 in the devastating rice blast fungus Magnaporthe oryzae by functional genomic approach.Compared to wild type,deletion of MoSEY1 considerably decreased the growth and conidia production of M.oryzae.Additionally,the absence of MoSEY1 delayed appressorium formation and invasive hyphae growth.The appressorium function was also impaired inΔMosey1 mutant.Subcellular localization analysis revealed that MoSey1 is localized at the ER.TheΔMosey1 mutant showed augmented sensitivity to ER stress.Additionally,we found that MoSey1 regulated the unfolded protein response,autophagy,and protein secretion in M.oryzae.In conclusion,our study unveiled the involvement of MoSey1 in the development,pathogenesis,and ER functions in M.oryzae.
