Objective: This study aims to investigate the drainage effect and clinical outcomes of negative pressure chest drainage in patients after two-port thoracoscopic valve surgery, comparing the differences in postoperativ...Objective: This study aims to investigate the drainage effect and clinical outcomes of negative pressure chest drainage in patients after two-port thoracoscopic valve surgery, comparing the differences in postoperative pain, hospital stay, and other factors between the negative pressure group and the control group. Methods: This study is a prospective controlled trial that selected patients undergoing two-port thoracoscopic valve surgery at a certain hospital from January 2019 to December 2024. Patients were randomly assigned to the control group and the negative pressure group using a random number table method. The control group consisted of 30 patients (20 males, 10 females, mean age 42.03 ± 12.89 years), and the negative pressure group consisted of 35 patients (26 males, 9 females, mean age 41.84 ± 11.83 years). The control group received traditional chest drainage, while the negative pressure group received negative pressure chest drainage. Postoperative pain scores, hospital stay, drainage time, number of tube blockages, and incidences of pneumothorax or subcutaneous emphysema were recorded and statistically analyzed. Results: The negative pressure group had a significantly shorter postoperative drainage time compared to the control group (49.09 ± 11.99 hours vs. 79.10 ± 7.32 hours, P < 0.001). The postoperative pain score was lower in the negative pressure group (4.49 ± 1.27 vs. 7.03 ± 0.85, P < 0.001), and the hospital stay was significantly shorter (9.83 ± 1.69 days vs. 14.73 ± 2.32 days, P < 0.001). The incidence of pneumothorax or subcutaneous emphysema was significantly lower in the negative pressure group than in the control group (14.29% vs. 56.67%, P = 0.0003). Conclusion: The application of negative pressure chest drainage in patients after two-port thoracoscopic valve surgery can effectively reduce postoperative pain, shorten hospital stay, and lower the incidence of tube blockage and pneumothorax, demonstrating good clinical outcomes.展开更多
Background:Phototherapies based on sunlight,infrared,ultraviolet,visible,and laser-based treatments present advantages like high curative effects,small invasion,and negligible adverse reactions in cancer treatment.We ...Background:Phototherapies based on sunlight,infrared,ultraviolet,visible,and laser-based treatments present advantages like high curative effects,small invasion,and negligible adverse reactions in cancer treatment.We aimed to explore the potential therapeutic effects of blue light emitting diode(LED)in human hepatoma cells and decipher the underlying cellular and molecular mechanisms.Methods:Wound healing and transwell assays were employed to probe the inhibition of the invasion and migration of hepatocellular carcinoma cells in the presence of blue LED.The sphere-forming test was used to evaluate the effect of LED blue light irradiation on cancer stem cell properties.Immunofluorescence and western blotting were used to detect the changes inγ-H2AX.The Cell Counting Kit-8 assay,5-ethynyl-2′-deoxyuridine staining,and colony formation assay were used to detect the combined effect of blue LED and sorafenib on cell proliferation inhibition.Results:We demonstrated that the irradiation of blue LED light in hepatoma cells could lead to cell proliferation reduction along with the increase of cell apoptosis.Simultaneously,blue LED irradiation also markedly suppressed the migration and invasion ability of human hepatoma cells.Sphere formation analysis further revealed the decreased cancer stemness of hepatoma cells upon blue LED irradiation.Mechanistically,blue LED irradiation significantly promoted the expression of the phosphorylation of the core histone protein H2AX(γ-H2AX),a sensitive molecular marker of DNA damage.In addition,we found that the combined treatment of blue LED irradiation and sorafenib increased cancer cell sensitivity to sorafenib.Conclusion:Collectively,we demonstrated that blue LED irradiation exhibited anti-tumor effects on liver cancer cells by inducing DNA damage and could enhance chemosensitivity of cancer cells,which represents a potential approach for human hepatoma treatment.展开更多
Objective: This study aims to compare the effects of different drainage tube diameters (22F vs. 26F) combined with negative pressure suction on patients after valve replacement surgery, including postoperative indicat...Objective: This study aims to compare the effects of different drainage tube diameters (22F vs. 26F) combined with negative pressure suction on patients after valve replacement surgery, including postoperative indicators and complications. Methods: A total of 104 patients undergoing valve replacement surgery were included and divided into a 22F group (45 patients) and a 26F group (59 patients). The basic characteristics, postoperative ICU stay duration, drainage duration, postoperative complications, and pain scores were compared between the two groups. All data were analyzed using SPSS statistical software, with p Results: There were no significant differences between the two groups in terms of age, sex, and underlying diseases. The ICU stay duration and drainage duration showed no significant differences (p > 0.05). The total drainage volume in the 22F group was significantly lower than that in the 26F group (225 vs. 380 ml, p = 0.035), and the pain scores on the third postoperative day were also significantly lower in the 22F group (p Conclusion: Compared to the 26F group, patients in the 22F group exhibited less postoperative drainage volume and lower pain scores, suggesting that the 22F drainage tube may have better clinical outcomes after valve replacement surgery.展开更多
Main observation and conclusion Herein,we report a highly chemo-and regioselective vicinal trifunctionalization of aryl iodides by palladium/norbornene(Pd/NBE)catalysis.The key feature of this new method is the introd...Main observation and conclusion Herein,we report a highly chemo-and regioselective vicinal trifunctionalization of aryl iodides by palladium/norbornene(Pd/NBE)catalysis.The key feature of this new method is the introduction of two distinct nitrogen and carbon electrophiles,with a large gap in reactivity,for ortho-unsubstituted aryl iodides via an intermolecular and intramolecular C―H functionalization,respectively.Eight types of ipso terminations can be coupled with both ortho-amination and ortho-alkylation,affording a variety of polysubstituted benzoheterocyclic scaffolds.Silicon-tethered substrates can lead to polyfucntional arenes via a single-step operation.Noteworthy,these products exhibit full-color-tunable strong fluorescence emissions with large Stokes shifts,and product 7r can serve as a fluorescent probe to specifically target lysosome in living cells.展开更多
The oxygen reduction reaction(ORR)is an important reaction in fuel cells and metal air batteries.The reaction is affected by slow kinetics and the use of high-priced and resource-scarce platinum-based catalysts.Theref...The oxygen reduction reaction(ORR)is an important reaction in fuel cells and metal air batteries.The reaction is affected by slow kinetics and the use of high-priced and resource-scarce platinum-based catalysts.Therefore,there is an urgent need to develop cost-effective nonprecious metal catalysts for use in the ORR to replace Pt-based catalysts.In this study,we used two-dimensional covalent organic frameworks(recorded as TF-COFs)as precursors to produce a metal-free nitrogen and fluorine-co-doped porous carbon catalyst(recorded as TF-800,TF-900,TF-1000,and TF-1100).TF-1000 had a high initial potential(0.90 V)and half-wave potential(0.83 V)in an alkaline medium,indicating good catalytic activity.In addition,the ORR stability of TF-1000 was better than that of commercial Pt/C(20%).This is mainly because the porous carbon catalyst has a high specific surface area,and the nitrogen and fluorine atoms in it have good dispersion and synergistic effects on the polarization of adjacent carbon atoms.TF-1000 also shows excellent performance in zinc-air batteries.In the TF-1000 based zinc-air batteries,excellent battery performance was observed:the peak power density reached 216.66 mW·cm^(-2),the specific capacity reached 752.86 mAh·g^(-1) at 10 mA·cm^(-2),and the long-term charge-discharge stability was demonstrated.This work provides inspiration for new strategies to prepare future ORR catalysts.展开更多
The development of covalent organic frameworks(COFs)with robust linkages is fundamentally important for the photocatalytic production of H_(2)O_(2).In this work,a series of isostructural COFs with robust imidazole lin...The development of covalent organic frameworks(COFs)with robust linkages is fundamentally important for the photocatalytic production of H_(2)O_(2).In this work,a series of isostructural COFs with robust imidazole linkage were synthesized as photocatalysts for H_(2)O_(2)production via the precise N-substituted microenvironment regulation(benzene,pyridine,pyrimidine,and triazine).The corresponding frameworks enable water and dissolved oxygen to reach the catalytic sites easily via planar skeletons and regulation of nitrogen-atom numbers.Additionally,the N-adjustment of heterocycle units in these COFs could significantly regulate the electronic band structures,light-harvesting capacity,and hydrophilic properties.The experimental investigation demonstrated that the photocatalytic process of COFs was composed of a dominant and indirect two-electron(2e^(-))oxygen reduction reaction(ORR).Notably,compared to H-COF(benzene),P-COF(pyridine),and M-COF(pyrimidine),T-COF with triazine unit exhibited the highest H_(2)O_(2)production rate of 42,180μmol·g^(-1)·h^(-1)due to its wider visible light absorption and higher separation efficiency of photogenerated electron-hole pairs.Theoretical investigations confirmed that N-heterocycle units in COFs could precisely modulate the energy barrier related to the formation of*OOH and*O_(2-).This study is expected to provide a new way for rationally designing imidazole-linked COFs as promising photocatalysts for efficiently photocatalytic H_(2)O_(2)generation.展开更多
Adding prebiotics to fermented dairy products is an effective method to simultaneously improve product quality and promote health.This study investigated how co-fermenting three oligosaccharides affected the quality o...Adding prebiotics to fermented dairy products is an effective method to simultaneously improve product quality and promote health.This study investigated how co-fermenting three oligosaccharides affected the quality of fermented goat milk(FGM)and its ability to regulate the small intestinal environment.The co-fermentation with three oligosaccharides increased the number of lactic acid bacteria(LAB)in the FGM and maintained a higher percentage of probiotic content during 35 d of storage compared to that of the control FGM without added sugars.The degree of acidification and textural attributes differed among the FGM samples,but the overall quality level increased in the samples fermented with only fructooligosaccharide(FOS)compared to that of the control.Moreover,complex probiotics(FGM)positively affected the small intestinal environment of mice by stabilizing the microbial population and promoting the growth of Bifidobacteria and butyric acid-producing bacteria,specifically Ruminococcus-gnavus.The butyric acid content was higher in the FOS-FGM group than in the other treatment groups.These results indicated that FOS-FGM is more suitable for the gut microbiota and influences short-chain fatty acid metabolism.This study is the first to investigate the effects of co-fermentation with three different oligosaccharides on the quality and small intestinal environment regulatory capacity of FGM products.展开更多
Cronobacter sakazakii is a foodborne pathogen,and the use of effective and non-hazardous bacteriostatic agents to manage C.sakazakii infection has garnered attention.Portulaca oleracea L.is a food plant with several p...Cronobacter sakazakii is a foodborne pathogen,and the use of effective and non-hazardous bacteriostatic agents to manage C.sakazakii infection has garnered attention.Portulaca oleracea L.is a food plant with several pharmaceutical properties.However,the products of kombucha-fermented P.oleracea(FP)have been scarcely studied with respect to their metabolite profiles and for their effects against food-borne pathogens.Therefore,in this study,the chemical and microbiological composition and inhibitory effects of FP against C.sakazakii were evaluated.The activities and molecular mechanisms of FP against C.sakazakii were investigated for the first time using transcriptomic and physiological experiments.Komagataeibacter was the most abundant bacterium in fermentation broth(>79%),and chlorogenic and acetic acids were the most abundant phenolics and organic acids in fermentation products.Transcriptomic analysis showed that FP induced significant changes in C.sakazakii as demonstrated by the differential expression of 506 genes.The genes that exhibited differential expression were primarily involved in ATP-related energy metabolism,cell membrane structure,protein biosynthesis,amino acid transport,and metabolism.These roles suggest multifaceted potential effects including cell membrane damage,protein synthesis limitation,metabolism disorder,reduced virulence factor expression,and reduced cell mobility.FP addition significantly inhibited C.sakazakii growth in PIF.These results provide insights into the anti-C.sakazakii mechanism of FP,which is valuable for its development and application in food safety.展开更多
文摘Objective: This study aims to investigate the drainage effect and clinical outcomes of negative pressure chest drainage in patients after two-port thoracoscopic valve surgery, comparing the differences in postoperative pain, hospital stay, and other factors between the negative pressure group and the control group. Methods: This study is a prospective controlled trial that selected patients undergoing two-port thoracoscopic valve surgery at a certain hospital from January 2019 to December 2024. Patients were randomly assigned to the control group and the negative pressure group using a random number table method. The control group consisted of 30 patients (20 males, 10 females, mean age 42.03 ± 12.89 years), and the negative pressure group consisted of 35 patients (26 males, 9 females, mean age 41.84 ± 11.83 years). The control group received traditional chest drainage, while the negative pressure group received negative pressure chest drainage. Postoperative pain scores, hospital stay, drainage time, number of tube blockages, and incidences of pneumothorax or subcutaneous emphysema were recorded and statistically analyzed. Results: The negative pressure group had a significantly shorter postoperative drainage time compared to the control group (49.09 ± 11.99 hours vs. 79.10 ± 7.32 hours, P < 0.001). The postoperative pain score was lower in the negative pressure group (4.49 ± 1.27 vs. 7.03 ± 0.85, P < 0.001), and the hospital stay was significantly shorter (9.83 ± 1.69 days vs. 14.73 ± 2.32 days, P < 0.001). The incidence of pneumothorax or subcutaneous emphysema was significantly lower in the negative pressure group than in the control group (14.29% vs. 56.67%, P = 0.0003). Conclusion: The application of negative pressure chest drainage in patients after two-port thoracoscopic valve surgery can effectively reduce postoperative pain, shorten hospital stay, and lower the incidence of tube blockage and pneumothorax, demonstrating good clinical outcomes.
基金supported by grants from the National Key Research and Development Program of China(2017YFB0403802)the Outstanding Youth Project of the Natural Science Foundation of Heilongjiang Province(YQ2020H019)the Huaier Fund in 2023 from Chen Xiao-Ping Foundation for the Development of Science and Technology of Hubei Province.
文摘Background:Phototherapies based on sunlight,infrared,ultraviolet,visible,and laser-based treatments present advantages like high curative effects,small invasion,and negligible adverse reactions in cancer treatment.We aimed to explore the potential therapeutic effects of blue light emitting diode(LED)in human hepatoma cells and decipher the underlying cellular and molecular mechanisms.Methods:Wound healing and transwell assays were employed to probe the inhibition of the invasion and migration of hepatocellular carcinoma cells in the presence of blue LED.The sphere-forming test was used to evaluate the effect of LED blue light irradiation on cancer stem cell properties.Immunofluorescence and western blotting were used to detect the changes inγ-H2AX.The Cell Counting Kit-8 assay,5-ethynyl-2′-deoxyuridine staining,and colony formation assay were used to detect the combined effect of blue LED and sorafenib on cell proliferation inhibition.Results:We demonstrated that the irradiation of blue LED light in hepatoma cells could lead to cell proliferation reduction along with the increase of cell apoptosis.Simultaneously,blue LED irradiation also markedly suppressed the migration and invasion ability of human hepatoma cells.Sphere formation analysis further revealed the decreased cancer stemness of hepatoma cells upon blue LED irradiation.Mechanistically,blue LED irradiation significantly promoted the expression of the phosphorylation of the core histone protein H2AX(γ-H2AX),a sensitive molecular marker of DNA damage.In addition,we found that the combined treatment of blue LED irradiation and sorafenib increased cancer cell sensitivity to sorafenib.Conclusion:Collectively,we demonstrated that blue LED irradiation exhibited anti-tumor effects on liver cancer cells by inducing DNA damage and could enhance chemosensitivity of cancer cells,which represents a potential approach for human hepatoma treatment.
文摘Objective: This study aims to compare the effects of different drainage tube diameters (22F vs. 26F) combined with negative pressure suction on patients after valve replacement surgery, including postoperative indicators and complications. Methods: A total of 104 patients undergoing valve replacement surgery were included and divided into a 22F group (45 patients) and a 26F group (59 patients). The basic characteristics, postoperative ICU stay duration, drainage duration, postoperative complications, and pain scores were compared between the two groups. All data were analyzed using SPSS statistical software, with p Results: There were no significant differences between the two groups in terms of age, sex, and underlying diseases. The ICU stay duration and drainage duration showed no significant differences (p > 0.05). The total drainage volume in the 22F group was significantly lower than that in the 26F group (225 vs. 380 ml, p = 0.035), and the pain scores on the third postoperative day were also significantly lower in the 22F group (p Conclusion: Compared to the 26F group, patients in the 22F group exhibited less postoperative drainage volume and lower pain scores, suggesting that the 22F drainage tube may have better clinical outcomes after valve replacement surgery.
基金supported by the National Natural Science Foundation of China(No.21925108)the Key Labora-of Xi'an(No.201805058ZD9CG42)。
文摘Main observation and conclusion Herein,we report a highly chemo-and regioselective vicinal trifunctionalization of aryl iodides by palladium/norbornene(Pd/NBE)catalysis.The key feature of this new method is the introduction of two distinct nitrogen and carbon electrophiles,with a large gap in reactivity,for ortho-unsubstituted aryl iodides via an intermolecular and intramolecular C―H functionalization,respectively.Eight types of ipso terminations can be coupled with both ortho-amination and ortho-alkylation,affording a variety of polysubstituted benzoheterocyclic scaffolds.Silicon-tethered substrates can lead to polyfucntional arenes via a single-step operation.Noteworthy,these products exhibit full-color-tunable strong fluorescence emissions with large Stokes shifts,and product 7r can serve as a fluorescent probe to specifically target lysosome in living cells.
基金supported by the National Natural Science Foundation of China(No.U22A20143,52103277)the Program for Science&Technology Innovation Talents in Universities of Henan Province(23HASTIT015)+2 种基金Henan Province science and technology research project(242102240071)certificate of postdoctoral research grant in Henan provinceZhongyuan Institute of Technology Superior Discipline Strength Enhancement Program.
文摘The oxygen reduction reaction(ORR)is an important reaction in fuel cells and metal air batteries.The reaction is affected by slow kinetics and the use of high-priced and resource-scarce platinum-based catalysts.Therefore,there is an urgent need to develop cost-effective nonprecious metal catalysts for use in the ORR to replace Pt-based catalysts.In this study,we used two-dimensional covalent organic frameworks(recorded as TF-COFs)as precursors to produce a metal-free nitrogen and fluorine-co-doped porous carbon catalyst(recorded as TF-800,TF-900,TF-1000,and TF-1100).TF-1000 had a high initial potential(0.90 V)and half-wave potential(0.83 V)in an alkaline medium,indicating good catalytic activity.In addition,the ORR stability of TF-1000 was better than that of commercial Pt/C(20%).This is mainly because the porous carbon catalyst has a high specific surface area,and the nitrogen and fluorine atoms in it have good dispersion and synergistic effects on the polarization of adjacent carbon atoms.TF-1000 also shows excellent performance in zinc-air batteries.In the TF-1000 based zinc-air batteries,excellent battery performance was observed:the peak power density reached 216.66 mW·cm^(-2),the specific capacity reached 752.86 mAh·g^(-1) at 10 mA·cm^(-2),and the long-term charge-discharge stability was demonstrated.This work provides inspiration for new strategies to prepare future ORR catalysts.
基金supported by the National Natural Science Foundation of China(Nos.U22A20143,52403288)the Natural Science Foundation of Henan Province(No.242300421073)+3 种基金the Program for Science&Technology Innovation Talents in Universities of Henan Province(No.23HASTIT015)Henan Provincial Science and Technology R&D Program Joint Fund(No.235200810071),Central Plains Elite Young Top-notch Talents,the Key Scientific and Technological Project of Henan Province(No.252102230152)Open Project Program of Yaoshan laboratory(No.2024004)Certificate of Postdoctoral Research Grant in Henan Province and Zhongyuan Institute of Technology Superior Discipline Strength Enhancement Program.
文摘The development of covalent organic frameworks(COFs)with robust linkages is fundamentally important for the photocatalytic production of H_(2)O_(2).In this work,a series of isostructural COFs with robust imidazole linkage were synthesized as photocatalysts for H_(2)O_(2)production via the precise N-substituted microenvironment regulation(benzene,pyridine,pyrimidine,and triazine).The corresponding frameworks enable water and dissolved oxygen to reach the catalytic sites easily via planar skeletons and regulation of nitrogen-atom numbers.Additionally,the N-adjustment of heterocycle units in these COFs could significantly regulate the electronic band structures,light-harvesting capacity,and hydrophilic properties.The experimental investigation demonstrated that the photocatalytic process of COFs was composed of a dominant and indirect two-electron(2e^(-))oxygen reduction reaction(ORR).Notably,compared to H-COF(benzene),P-COF(pyridine),and M-COF(pyrimidine),T-COF with triazine unit exhibited the highest H_(2)O_(2)production rate of 42,180μmol·g^(-1)·h^(-1)due to its wider visible light absorption and higher separation efficiency of photogenerated electron-hole pairs.Theoretical investigations confirmed that N-heterocycle units in COFs could precisely modulate the energy barrier related to the formation of*OOH and*O_(2-).This study is expected to provide a new way for rationally designing imidazole-linked COFs as promising photocatalysts for efficiently photocatalytic H_(2)O_(2)generation.
基金supported by Shaanxi science and tech-nology plan projects of China(2022KXJ-010,2022ZDLNY04-09)Xi'an city science and technology plan projects of China(22NYGG0012,23KGDW0021-2022)+1 种基金Science and technology plan projects in Xianyang city of Shaanxi Province(2021ZDZX-NY-0014)the Fundamental Research Funds for the Central Universities in China(GK202306005).
文摘Adding prebiotics to fermented dairy products is an effective method to simultaneously improve product quality and promote health.This study investigated how co-fermenting three oligosaccharides affected the quality of fermented goat milk(FGM)and its ability to regulate the small intestinal environment.The co-fermentation with three oligosaccharides increased the number of lactic acid bacteria(LAB)in the FGM and maintained a higher percentage of probiotic content during 35 d of storage compared to that of the control FGM without added sugars.The degree of acidification and textural attributes differed among the FGM samples,but the overall quality level increased in the samples fermented with only fructooligosaccharide(FOS)compared to that of the control.Moreover,complex probiotics(FGM)positively affected the small intestinal environment of mice by stabilizing the microbial population and promoting the growth of Bifidobacteria and butyric acid-producing bacteria,specifically Ruminococcus-gnavus.The butyric acid content was higher in the FOS-FGM group than in the other treatment groups.These results indicated that FOS-FGM is more suitable for the gut microbiota and influences short-chain fatty acid metabolism.This study is the first to investigate the effects of co-fermentation with three different oligosaccharides on the quality and small intestinal environment regulatory capacity of FGM products.
基金financially supported by Shaanxi science and technology plan projects of China(2022KXJ-010,2022ZDLNY04-09)Xi’an city science and technology plan projects of China(22NYGG0012,23KGDW0021-2022)the Fundamental Research Funds for the Central Universities in China(GK202306005).
文摘Cronobacter sakazakii is a foodborne pathogen,and the use of effective and non-hazardous bacteriostatic agents to manage C.sakazakii infection has garnered attention.Portulaca oleracea L.is a food plant with several pharmaceutical properties.However,the products of kombucha-fermented P.oleracea(FP)have been scarcely studied with respect to their metabolite profiles and for their effects against food-borne pathogens.Therefore,in this study,the chemical and microbiological composition and inhibitory effects of FP against C.sakazakii were evaluated.The activities and molecular mechanisms of FP against C.sakazakii were investigated for the first time using transcriptomic and physiological experiments.Komagataeibacter was the most abundant bacterium in fermentation broth(>79%),and chlorogenic and acetic acids were the most abundant phenolics and organic acids in fermentation products.Transcriptomic analysis showed that FP induced significant changes in C.sakazakii as demonstrated by the differential expression of 506 genes.The genes that exhibited differential expression were primarily involved in ATP-related energy metabolism,cell membrane structure,protein biosynthesis,amino acid transport,and metabolism.These roles suggest multifaceted potential effects including cell membrane damage,protein synthesis limitation,metabolism disorder,reduced virulence factor expression,and reduced cell mobility.FP addition significantly inhibited C.sakazakii growth in PIF.These results provide insights into the anti-C.sakazakii mechanism of FP,which is valuable for its development and application in food safety.
