Reducing aerobic spoilage and rumen greenhouse gas emissions from anaerobically fermented feeds remains critical challenges in energy saving and environmental protection of animal husbandry.This study investigated the...Reducing aerobic spoilage and rumen greenhouse gas emissions from anaerobically fermented feeds remains critical challenges in energy saving and environmental protection of animal husbandry.This study investigated the effects of dandelions,both alone and combined with Lactobacillus plantarum and Lactobacillus buchneri(LAB)on fermentation quality,bacteriome and mycobiome after 180 d of anaerobic and 4 d of aerobic fermentation of whole-plant corn.In vitro dry matter digestion(IVDMD)and gas production from anaerobically fermented whole-plant corn were also assessed.The results demonstrated that dandelions,either alone or combined with LAB,effectively improved fermentation quality by reducing NH_(3)-N concentrations(22.72–25.99%)after anaerobic fermentation,decreasing the proliferation of yeast and molds to enhance the aerobic stability.Notably,the changes in the bacteriome were more pronounced than those in the mycobiome after aerobic exposure.The addition of dandelions or the combination reduced Acetobacter fabarum abundance,a member of the Acetobacter that was spoilage-induced microbe indicated by correlation analysis.Besides,these treatments facilitated competition relations of microbiome which contributed to the enhanced aerobic stability.Furthermore,dandelions reduced CH_(4)and CO_(2)emissions by 14.88 and 13.73%,respectively,and also positively influencing IVDMD by 4.46%.Collectively,dandelion alone or combined with LAB are promising strategies to improve the aerobic stability of anaerobically fermented whole-plant corn,a process linked to the interactions between the bacteriome and mycobiome,and to contribute to clean production by reducing rumen CH_(4)and CO_(2)emissions.展开更多
The effects of Lactobacillus plantarum in microencapsulation(LPM)on intestinal development in layer chicks were investigated in this study,as well as the colonization of L.plantarum in the gut.A total of 480 healthy H...The effects of Lactobacillus plantarum in microencapsulation(LPM)on intestinal development in layer chicks were investigated in this study,as well as the colonization of L.plantarum in the gut.A total of 480 healthy Hy-Line Brown layer chicks at 0 d old were randomly divided into 4 groups(8 replicates each treatment),and the diets of these birds were supplemented with nothing(control),L.plantarum(0.02 g/kg feed;10^(9) CFU/kg feed),LPM(1.0 g/kg feed;109 CFU/kg feed)and wall material of LPM(WM;0.98 g/kg feed),respectively.Compared to control,LPM improved growth performance and intestinal development of layer chicks,evidenced by significantly increased body weight,average daily gain,average daily feed intake,villus height,villus height/crypt depth,as well as weight and length of the duodenum,jejunum and ileum(P<0.05).These results could be attributed to the increased colonization of L.plantarum in the gut,which was verified by significant increases in lactic acid content,viable counts in chyme and mucosa(P<0.05),as well as a visible rise in number of strains labeled with fluorescein isothiocyanate.Meanwhile,the relative abundances of Lactobacillus and Bifidobacterium significantly increased in response to microencapsulated L.plantarum supplementation(P<0.05),accompanied by the significant up-regulation of colonization related genes(P<0.05),encoding solute carrier family,monocarboxylate transporter,activin A receptor,succinate receptor and secretogranin II.To sum up,microencapsulated L.plantarum supplementation promoted intestinal development,which could be attributed to the enhancement of L.plantarum colonization in the intestine through the mutual assistance of Bifidobacterium and interactions with colonization related transmembrane proteins.展开更多
Liver fibrosis, resulting from chronic liver damage and characterized by the accumulation of extracellular matrix (ECM) proteins, is a characteristic of most types of chronic liver diseases. The activation of hepatic ...Liver fibrosis, resulting from chronic liver damage and characterized by the accumulation of extracellular matrix (ECM) proteins, is a characteristic of most types of chronic liver diseases. The activation of hepatic stellate cells (HSC) is considered an essential pathological hallmark in liver fibrosis. Although nitric oxide (NO) can effectively induce HSC apoptosis, the systemic administration of NO is ineffective and may cause severe complications such as hypotension. To overcome this limitation, nanoparticles were designed to target HSCs and release NO locally under the exposure of near infrared light (NIR). To achieve this, upconversion nanoparticle (UCNP) cores were enveloped in mesoporous silica shells (UCNP@mSiO2), which were modified with hyaluronic acid (HA-UCNP@mSiO2) and Roussin’s black salt (RBS). HA molecules recognize and bind to CD44 proteins, which are overexpressed on activated HSCs. Under exposure to a 980-nm NIR laser, the UCNP cores convert the 980-nm wavelength into ultraviolet (UV) light, which then energizes the RBS (NO donors), resulting in an efficient release of NO inside of the HSCs. Once released, NO triggers HSC apoptosis and reverses the liver fibrosis. This targeted and controlled release method provides the theoretical and experimental basis for novel therapeutic approaches to treat hepatic fibrosis.展开更多
基金supported by the Henan Provincial Science and Technology Research Project,China(242102111014)the Key Scientific Research Project of Colleges and Universities in Henan Province,China(23A230003)the Cultivation Program for Young Key Teachers and the Scientific Research Foundation for High-level Talents in Henan University of Technology,China(31401489)。
文摘Reducing aerobic spoilage and rumen greenhouse gas emissions from anaerobically fermented feeds remains critical challenges in energy saving and environmental protection of animal husbandry.This study investigated the effects of dandelions,both alone and combined with Lactobacillus plantarum and Lactobacillus buchneri(LAB)on fermentation quality,bacteriome and mycobiome after 180 d of anaerobic and 4 d of aerobic fermentation of whole-plant corn.In vitro dry matter digestion(IVDMD)and gas production from anaerobically fermented whole-plant corn were also assessed.The results demonstrated that dandelions,either alone or combined with LAB,effectively improved fermentation quality by reducing NH_(3)-N concentrations(22.72–25.99%)after anaerobic fermentation,decreasing the proliferation of yeast and molds to enhance the aerobic stability.Notably,the changes in the bacteriome were more pronounced than those in the mycobiome after aerobic exposure.The addition of dandelions or the combination reduced Acetobacter fabarum abundance,a member of the Acetobacter that was spoilage-induced microbe indicated by correlation analysis.Besides,these treatments facilitated competition relations of microbiome which contributed to the enhanced aerobic stability.Furthermore,dandelions reduced CH_(4)and CO_(2)emissions by 14.88 and 13.73%,respectively,and also positively influencing IVDMD by 4.46%.Collectively,dandelion alone or combined with LAB are promising strategies to improve the aerobic stability of anaerobically fermented whole-plant corn,a process linked to the interactions between the bacteriome and mycobiome,and to contribute to clean production by reducing rumen CH_(4)and CO_(2)emissions.
基金financed by the National Key Research and Development Program of China (2021YFD1300300)the National Natural Science Foundation of China (32202697)+1 种基金Key Technology Research and Development Program of Henan Province (232102111041)the Innovation Fund of Henan University of Technology (2022ZKCJ17).
文摘The effects of Lactobacillus plantarum in microencapsulation(LPM)on intestinal development in layer chicks were investigated in this study,as well as the colonization of L.plantarum in the gut.A total of 480 healthy Hy-Line Brown layer chicks at 0 d old were randomly divided into 4 groups(8 replicates each treatment),and the diets of these birds were supplemented with nothing(control),L.plantarum(0.02 g/kg feed;10^(9) CFU/kg feed),LPM(1.0 g/kg feed;109 CFU/kg feed)and wall material of LPM(WM;0.98 g/kg feed),respectively.Compared to control,LPM improved growth performance and intestinal development of layer chicks,evidenced by significantly increased body weight,average daily gain,average daily feed intake,villus height,villus height/crypt depth,as well as weight and length of the duodenum,jejunum and ileum(P<0.05).These results could be attributed to the increased colonization of L.plantarum in the gut,which was verified by significant increases in lactic acid content,viable counts in chyme and mucosa(P<0.05),as well as a visible rise in number of strains labeled with fluorescein isothiocyanate.Meanwhile,the relative abundances of Lactobacillus and Bifidobacterium significantly increased in response to microencapsulated L.plantarum supplementation(P<0.05),accompanied by the significant up-regulation of colonization related genes(P<0.05),encoding solute carrier family,monocarboxylate transporter,activin A receptor,succinate receptor and secretogranin II.To sum up,microencapsulated L.plantarum supplementation promoted intestinal development,which could be attributed to the enhancement of L.plantarum colonization in the intestine through the mutual assistance of Bifidobacterium and interactions with colonization related transmembrane proteins.
基金This work was supported by the American Heart Association(Nos.18TPA34230092 and 19EIA34660286 to K.C.)the National Natural Science Foundation of China(No.U1904149 to H.X.L.)+1 种基金National S&T Major Project of China(No.2018ZX10301201-008 to Z.G.R.)the High Technology Research and Development Program of Henan Province(No.20A320055 to H.X.L.).
文摘Liver fibrosis, resulting from chronic liver damage and characterized by the accumulation of extracellular matrix (ECM) proteins, is a characteristic of most types of chronic liver diseases. The activation of hepatic stellate cells (HSC) is considered an essential pathological hallmark in liver fibrosis. Although nitric oxide (NO) can effectively induce HSC apoptosis, the systemic administration of NO is ineffective and may cause severe complications such as hypotension. To overcome this limitation, nanoparticles were designed to target HSCs and release NO locally under the exposure of near infrared light (NIR). To achieve this, upconversion nanoparticle (UCNP) cores were enveloped in mesoporous silica shells (UCNP@mSiO2), which were modified with hyaluronic acid (HA-UCNP@mSiO2) and Roussin’s black salt (RBS). HA molecules recognize and bind to CD44 proteins, which are overexpressed on activated HSCs. Under exposure to a 980-nm NIR laser, the UCNP cores convert the 980-nm wavelength into ultraviolet (UV) light, which then energizes the RBS (NO donors), resulting in an efficient release of NO inside of the HSCs. Once released, NO triggers HSC apoptosis and reverses the liver fibrosis. This targeted and controlled release method provides the theoretical and experimental basis for novel therapeutic approaches to treat hepatic fibrosis.
