High sensitive,accurate detection for tumor-associated overexpressed enzyme activity is highly significant for further understanding enzyme function,discovering potential drugs,and early diagnosis and prevention of di...High sensitive,accurate detection for tumor-associated overexpressed enzyme activity is highly significant for further understanding enzyme function,discovering potential drugs,and early diagnosis and prevention of diseases.In this work,we developed a facile,direct and single-step detection platform for primary ovarian cancers related glycosidase activity based on the inner filter effect(IFE)between glycosidase catalytic product and black phosphorus quantum dots(BPQDs).Highly fluorescent BPQDs were successfully synthesized from bulk black phosphorus by a simple liquid exfoliation method.Under the catalysis ofβ-galactosidase,p-nitrophenyl-β-D-galactopyranoside(PNPG)was transformed into pnitrophenol(PNP)andβ-D-galactopyranoside.Meanwhile,the absorption of catalytic product PNP greatly overlapped with the excitation and emission spectra of fluorescent BPQDs,leading to the fluorescence quenching of BPQDs with a high quenching efficiency.The proposed sensing strategy provided a low detection limit of 0.76 U/L,which was 1-2 orders of magnitude lower than most unmodified sensing platforms.D-Galactal was selected as the inhibitor forβ-galactosidase to further assess the feasibility of screening potential inhibitors.The fluorescence recovery of BPQDs suggests that the unmodified sensing platform is feasible to discover potential drugs ofβ-galactosidase.Our work paves a general way in the detection of glycosidase activity with fluorescent BPQDs,which can be promising for glycosidase-related disease diagnosis and pathophysiology elucidation.展开更多
Nature has created complex living systems with outstanding structure and remarkable function.Constructing biomimetic systems that rival living organisms has attracted considerable research inter-est in research fields...Nature has created complex living systems with outstanding structure and remarkable function.Constructing biomimetic systems that rival living organisms has attracted considerable research inter-est in research fields of self-assembly and bionic sci-ence.展开更多
基金supported by the National Key R&D Program of China(No.2017YFA0208000)the National Natural Science Foundation of China(No.21675120)+1 种基金Ten Thousand Talents Program for Young Talents,the Foundation for Innovative Research Groups of the National Nature Science Foundation of China(No.21521063)the Start-up Research Fund for Prof.Q.Yuan(Nos.531107050973,531109010053)
文摘High sensitive,accurate detection for tumor-associated overexpressed enzyme activity is highly significant for further understanding enzyme function,discovering potential drugs,and early diagnosis and prevention of diseases.In this work,we developed a facile,direct and single-step detection platform for primary ovarian cancers related glycosidase activity based on the inner filter effect(IFE)between glycosidase catalytic product and black phosphorus quantum dots(BPQDs).Highly fluorescent BPQDs were successfully synthesized from bulk black phosphorus by a simple liquid exfoliation method.Under the catalysis ofβ-galactosidase,p-nitrophenyl-β-D-galactopyranoside(PNPG)was transformed into pnitrophenol(PNP)andβ-D-galactopyranoside.Meanwhile,the absorption of catalytic product PNP greatly overlapped with the excitation and emission spectra of fluorescent BPQDs,leading to the fluorescence quenching of BPQDs with a high quenching efficiency.The proposed sensing strategy provided a low detection limit of 0.76 U/L,which was 1-2 orders of magnitude lower than most unmodified sensing platforms.D-Galactal was selected as the inhibitor forβ-galactosidase to further assess the feasibility of screening potential inhibitors.The fluorescence recovery of BPQDs suggests that the unmodified sensing platform is feasible to discover potential drugs ofβ-galactosidase.Our work paves a general way in the detection of glycosidase activity with fluorescent BPQDs,which can be promising for glycosidase-related disease diagnosis and pathophysiology elucidation.
基金This work was supported by the National Key R&D Pro-gram of China(2017YFA0208000)the National Natural Science Foundation of China(21675120 and 31701249)+1 种基金the Foundation for Innovative Research Groups of NSFC(grant 21521063)the Keypoint Research and Inven-tion Program of Hunan province(2017DK2011).
文摘Nature has created complex living systems with outstanding structure and remarkable function.Constructing biomimetic systems that rival living organisms has attracted considerable research inter-est in research fields of self-assembly and bionic sci-ence.
