Bacterial infection and excessive inflammation are still the main obstacles to wound repair.Thus,antibacterial and antiinflammation nanomaterials are always attracting for infected wound healing.In this work,ultra-uni...Bacterial infection and excessive inflammation are still the main obstacles to wound repair.Thus,antibacterial and antiinflammation nanomaterials are always attracting for infected wound healing.In this work,ultra-uniform(-20 nm)and colloidally stable Ag nanoparticles(Ag-Hes NPs)with core-shell structure were prepared by using hesperidin as reducing and capping agent.The obtained Ag-Hes NPs present effective antibacterial properties on both Staphylococcus aureus and Escherichia coli.Ag-Hes NPs also got high 1,1-diphenyl-1-picrylhydrazyl scavenging capability of 69%.Under the package of polyvinyl alcohol and sodium alginate,Ag-Hes NPs were encapsulated into electro spun nanofibers to form hydrogel(Ag-Hes@H).This strategy provides a moisture environment which could enrich and release Ag-Hes NPs gradually.Cell experiments and animal wound healing investigation proved that Ag-Hes@H could promote the proliferation and migration of human umbilical vein endothelial cells and accelerate infected wound healing.Meanwhile,Ag-Hes@H significantly reduced the expression of inflammatory cytokines,including IL-6,MMP9 and TNF-α.Immunohistochemistry data further suggested that Ag-Hes@H accelerated wound closure by promoting collagen deposition and skin cell proliferation.The designed antibacterial and anti-inflammatory Ag-Hes@H has great potential for promoting infected wound healing.展开更多
Arthritis is a kind of chronic inflammatory autoimmune disease,which can destroy joint cartilage and bone,leading to joint pain,joint swelling,and limited mobility.Traditional therapies have many side effects or focus...Arthritis is a kind of chronic inflammatory autoimmune disease,which can destroy joint cartilage and bone,leading to joint pain,joint swelling,and limited mobility.Traditional therapies have many side effects or focus too much on anti-inflammation while neglecting joint repair.In this experiment,we combined Epigallocatechin gallate(EGCG)with extracellular vesicles derived from macrophages to treat rheumatoid arthritis.Sustained-release resulted in a significant decrease in chondrocyte expression of hypoxia-inducible factor 1-alpha,a decrease in apoptosis-related proteins Cytochrome C,Caspase-3,Caspase-9,and Bax.Molecular biological analysis showed that extracellular vesicles-encapsulated EGCG(EVs-EGCG)more significantly upregulated type II collagen expression by about 1.8-fold than EGCG alone,which was more beneficial for arthritis repair.Animal experiments revealed that these EGCG-coated extracellular vesicles significantly reduced swelling,decreased synovial hyperplasia,repaired cartilage,and attenuated arthritis-related pathology scores in arthritic rats.Measurement data showed that EVs-EGCG treatment reduced joint swelling by approximately 39.5%in rheumatoid rats.In vitro studies have shown that this EVs-EGCG can increase the expression of cartilage type II collagen and reduce apoptosis of chondrocytes.Moreover,it was demonstrated in vivo experiments to reduce cartilage destruction in rheumatoid arthritis rats,providing a solution for the treatment of rheumatoid arthritis.展开更多
基金supported by National Natural Science Foundation of China(No.82072076)2021 scientific research fund project of Liaoning Provincial Department of Education(LJKZ0819).
文摘Bacterial infection and excessive inflammation are still the main obstacles to wound repair.Thus,antibacterial and antiinflammation nanomaterials are always attracting for infected wound healing.In this work,ultra-uniform(-20 nm)and colloidally stable Ag nanoparticles(Ag-Hes NPs)with core-shell structure were prepared by using hesperidin as reducing and capping agent.The obtained Ag-Hes NPs present effective antibacterial properties on both Staphylococcus aureus and Escherichia coli.Ag-Hes NPs also got high 1,1-diphenyl-1-picrylhydrazyl scavenging capability of 69%.Under the package of polyvinyl alcohol and sodium alginate,Ag-Hes NPs were encapsulated into electro spun nanofibers to form hydrogel(Ag-Hes@H).This strategy provides a moisture environment which could enrich and release Ag-Hes NPs gradually.Cell experiments and animal wound healing investigation proved that Ag-Hes@H could promote the proliferation and migration of human umbilical vein endothelial cells and accelerate infected wound healing.Meanwhile,Ag-Hes@H significantly reduced the expression of inflammatory cytokines,including IL-6,MMP9 and TNF-α.Immunohistochemistry data further suggested that Ag-Hes@H accelerated wound closure by promoting collagen deposition and skin cell proliferation.The designed antibacterial and anti-inflammatory Ag-Hes@H has great potential for promoting infected wound healing.
基金supported via National Natural Science Foundation of China(No.82072076,81771987 and 82072165)Natural Science Foundation of Liaoning Province(No.201602309).
文摘Arthritis is a kind of chronic inflammatory autoimmune disease,which can destroy joint cartilage and bone,leading to joint pain,joint swelling,and limited mobility.Traditional therapies have many side effects or focus too much on anti-inflammation while neglecting joint repair.In this experiment,we combined Epigallocatechin gallate(EGCG)with extracellular vesicles derived from macrophages to treat rheumatoid arthritis.Sustained-release resulted in a significant decrease in chondrocyte expression of hypoxia-inducible factor 1-alpha,a decrease in apoptosis-related proteins Cytochrome C,Caspase-3,Caspase-9,and Bax.Molecular biological analysis showed that extracellular vesicles-encapsulated EGCG(EVs-EGCG)more significantly upregulated type II collagen expression by about 1.8-fold than EGCG alone,which was more beneficial for arthritis repair.Animal experiments revealed that these EGCG-coated extracellular vesicles significantly reduced swelling,decreased synovial hyperplasia,repaired cartilage,and attenuated arthritis-related pathology scores in arthritic rats.Measurement data showed that EVs-EGCG treatment reduced joint swelling by approximately 39.5%in rheumatoid rats.In vitro studies have shown that this EVs-EGCG can increase the expression of cartilage type II collagen and reduce apoptosis of chondrocytes.Moreover,it was demonstrated in vivo experiments to reduce cartilage destruction in rheumatoid arthritis rats,providing a solution for the treatment of rheumatoid arthritis.
