Nereididae is a prolific annelid family widely distributed in the world oceans,especially in the Indo-Pacific Convergence Zone(IPCZ).However,its biogeographic pattern remains unexplored in IPCZ.To contribute to the un...Nereididae is a prolific annelid family widely distributed in the world oceans,especially in the Indo-Pacific Convergence Zone(IPCZ).However,its biogeographic pattern remains unexplored in IPCZ.To contribute to the understanding of biodiversity and biogeography of Nereididae in the IPCZ,we integrated historical data of species distributions with those of model-predicted ones to determine the biogeographic patterns of nereid species,from which we projected to its future distribution patterns for 2090-2100 under different climate scenarios(SSP1-1.9 and SSP5-8.5).Functional diversity within IPCZ was assessed using functional richness,functional evenness,and functional disparity.Divergence times within Nereididae were estimated using three DNA marker genes(COI,16S,and 18S rRNA),and a time tree was constructed based on a strict molecular clock model.The IPCZ was established as a key Nereididae biodiversity hotspot through distribution modelling of 256 species(44 genera),and temperature emerging as the predominant climatic driver of species distribution patterns.The distribution of species and functional diversity is notable for its non-centralized pattern.We projected that by the end of the century,areas of medium-to-high species richness will expand significantly under the low-emission SSP1-1.9 climate scenario.However,under the high-emission SSP5-8.5 scenario,the suitability of these regions significantly declines,posing an increasingly severe threat to biodiversity.In addition,by molecular clock analysis,we revealed that the evolutionary divergence of extant nereidid species occurred mainly in the Cretaceous and Jurassic,suggesting that paleogeographical and environmental events,such as oceanic anoxic events,might have played a pivotal role in shaping the evolutionary trajectory and ecological adaptations of marine annelids.These findings highlight the importance of considering both current biodiversity patterns and historical contexts in conservation planning,and provided insights into the potential factors on the biogeographic distribution and evolutionary processes of Nereididae.展开更多
Atmospheric carbon dioxide(CO_(2))levels are escalating at an unprecedented rate,leading to the phenomenon of ocean acidification(OA).Parental exposure to acidification has the potential to enhance offspring resilienc...Atmospheric carbon dioxide(CO_(2))levels are escalating at an unprecedented rate,leading to the phenomenon of ocean acidification(OA).Parental exposure to acidification has the potential to enhance offspring resilience through cross-generation plasticity.In this study,we analyzed larval growth and transcriptomic profiles in the Pacific oyster,Crassostrea gigas,a species of significant ecological relevance,under both control and elevated CO_(2)conditions experienced by their parental generation.Our findings indicate that the oyster populations exposed to OA exhibited a higher incidence of abnormalities during the D-shaped larval stage,followed by accelerated growth at the eyed stage.Through a comparative transcriptomic investigation of eyed larvae(25 d after fertilization),we observed that parental exposure to OA substantially influenced the gene expression in the offspring.Genes associated with lipid catabolism and shell formation were notably upregulated in oysters with parental OA exposure,potentially playing a role in cross-generational conditioning and conferring resilience to OA stressors.These results underscore the profound impact of OA on oyster larval development via cross-generational mechanisms and shed light on the molecular underpinnings of cross-generation plasticity.展开更多
Recently,Internet ofThings(IoT)has been increasingly integrated into the automotive sector,enabling the development of diverse applications such as the Internet of Vehicles(IoV)and intelligent connected vehicles.Lever...Recently,Internet ofThings(IoT)has been increasingly integrated into the automotive sector,enabling the development of diverse applications such as the Internet of Vehicles(IoV)and intelligent connected vehicles.Leveraging IoVtechnologies,operational data fromcore vehicle components can be collected and analyzed to construct fault diagnosis models,thereby enhancing vehicle safety.However,automakers often struggle to acquire sufficient fault data to support effective model training.To address this challenge,a robust and efficient federated learning method(REFL)is constructed for machinery fault diagnosis in collaborative IoV,which can organize multiple companies to collaboratively develop a comprehensive fault diagnosis model while keeping their data locally.In the REFL,the gradient-based adversary algorithm is first introduced to the fault diagnosis field to enhance the deep learning model robustness.Moreover,the adaptive gradient processing process is designed to improve the model training speed and ensure the model accuracy under unbalance data scenarios.The proposed REFL is evaluated on non-independent and identically distributed(non-IID)real-world machinery fault dataset.Experiment results demonstrate that the REFL can achieve better performance than traditional learning methods and are promising for real industrial fault diagnosis.展开更多
Synergy strategy of photocatalysts and polymer resins are promising technology for marine antifouling.However,it is still a main challenge to obtain a green,safe,and efficient antifouling coatings.Herein,carbon(graphe...Synergy strategy of photocatalysts and polymer resins are promising technology for marine antifouling.However,it is still a main challenge to obtain a green,safe,and efficient antifouling coatings.Herein,carbon(graphene or CNT)modified Ti O_(2)photocatalyst was synthesized via hydrothermal and annealing process and has successfully applied in acrylate fluoroboron polymer(ABFP)composite coating.Morphology and chemical composition were detailed characterized.The graphene or CNT acted as a bridge with supplemental spatial structures(petal gaps,entanglement)and new functional groups(C-O,C-Ti-O,etc.)on Ti O_(2)particle.Carbon nanotube(CNT)modified TiO_(2)-ABFP coatings(BTCP)achieved excellent antibacterial and anti-diatom adhesion rate of 89.3%-96.70%and 99.00%-99.50%,which was 1.84-4.94-fold more than that of the single ABFP.CNT or graphene served as electronic bridges was considered as the crucial mechanism,which significantly improved the light absorption range and capacity,conductivity,and photoelectric response of Ti O_(2),and further accelerated the generation and transfer of free radicals to the surface of BTCP or FTGP.Moreover,the improvement of catalyst activity synergizes with the smooth surface,hydrophilicity,and slow hydrolysis of composite coatings,achieved long-term and efficient antifouling performance.This work provides a new insight into the modification of Ti O_(2)and antifouling mechanism of polymer coating.展开更多
Objective: To study the mechanism of Sijunzi decoction treating limb weakness in spleen Qi deficiency (SQD) based on the myonuclear domain (MND) theory. Methods: 40 male Sprague-Dawley rats were randomly divided into ...Objective: To study the mechanism of Sijunzi decoction treating limb weakness in spleen Qi deficiency (SQD) based on the myonuclear domain (MND) theory. Methods: 40 male Sprague-Dawley rats were randomly divided into the normal group, SQD model group (model group), SQD+ still water group (SW group) and SQD+ Sijunzi decoction group (CM group), 10 rats each group;Grip-Strength Meter was used to measure limb grip strength;transmission electron microscope was employed to observe the ultrastructural changes of the myofibers, Image Pro 6.0 was used to measure the myonuclear numbers, cross-section area (CSA) and then their ratios (the MND sizes) were calculated, immunofluorescence assay was chosen to test the expressions of paired box gene 7 (Pax7) and myogenic differentiation antigen (MyoD). Results: Compared with those in the normal group, limb grip strength was decreased, sarcomeres were abnormal, and all the myonuclear numbers, CSA and MND sizes were reduced, but the Pax7+ cell numbers were increased, significantly, in the model and SW groups;Compared with those in the model and SW groups, limb grip strength was increased, sarcomeres were basically normal, the myonuclear number and CSA were both greater, and the Pax7+ and MyoD+ cell numbers were both increased, significantly, in the CM group. Conclusion: Sijunzi decoction might increase the myonuclear number by activating the MSCs to treat limb weakness in SQD.展开更多
株型是影响谷类作物产量的重要性状,株型改良对提高作物产量具有重要意义。独脚金内酯(strigolactones,SLs)作为一种最新被鉴定的植物激素,其通过抑制腋芽的伸长调控分枝/分蘖的形成。β-胡萝卜素异构酶(D27s)是SLs合成途径的关键酶,通...株型是影响谷类作物产量的重要性状,株型改良对提高作物产量具有重要意义。独脚金内酯(strigolactones,SLs)作为一种最新被鉴定的植物激素,其通过抑制腋芽的伸长调控分枝/分蘖的形成。β-胡萝卜素异构酶(D27s)是SLs合成途径的关键酶,通过对谷子(Setaria italica)β-胡萝卜素异构酶典型结构域Pfam:DUF4033进行分析,鉴定到3个谷子D27s基因家族成员(Seita.8G168400、Seita.6G088800和Seita.3G050900)。蛋白质特性分析显示,谷子D27s蛋白由271–277个氨基酸残基组成,分子量为30.1–30.4 k Da,等电点为5.85–9.31,不稳定系数介于38.48–74.47之间,且均定位于叶绿体;系统进化分析发现,谷子D27s家族成员位于3个不同进化分支;顺式作用元件预测显示,SiD27-1(Seita.8G168400)可能参与调控生物节律、生长素介导的生长发育以及干旱和低温等胁迫应答过程。基因表达分析显示,SiD27-1在谷子多分蘖材料中表达下调,在低磷胁迫处理下,D27s基因均能产生不同程度的响应,并且Si D27-1的响应较其它成员更快速。单倍型分析结果表明,SiD27-1的H001单倍型为优异单倍型,对谷子的株高、抽穗期和产量改良具有重要应用价值。综上,推测SiD27-1极可能在SLs合成中发挥关键作用并对谷子株型产生影响。研究结果为深入揭示D27s对谷子分蘖形成的调控机制奠定了基础,也为谷子株型分子设计育种提供了优异的等位变异位点。展开更多
Plate culture counting and strain isolation methods were utilized to assess the species richness and abundance of planktonic and attached bacteria on glass plates in the surface and bottom seawater of Qingdao Middle H...Plate culture counting and strain isolation methods were utilized to assess the species richness and abundance of planktonic and attached bacteria on glass plates in the surface and bottom seawater of Qingdao Middle Harbor over a year,with monthly and quarterly sampling.Both species richness and bacterial numbers exhibited seasonal variations.Specifically,the abundance of attached bacteria and bacterioplankton peaked in June and July,corresponding to higher water temperatures in summer and autumn,while lower abundances were noted in January and December during cooler periods.Throughout the year,the species richness of attached bacteria consistently exceeded that of planktonic bacteria in both shallow and deep waters.Pseudoalteromonas emerged as the most prevalent genus among both planktonic and attached bacteria in surface and bottom seawater samples.Furthermore,the magnitude of changes in species richness and abundance for attached bacteria(0.66×10^(5)-15.85×10^(5)CFU/cm^(2))was greater than that observed for planktonic bacteria(0.58×10^(8)-5.33×10^(8)CFU/L).We propose that the attached bacterial populations,situated in limited microenvironments within the larger seawater ecosystem,exhibit heightened sensitivity to environmental fluctuations,resulting in more rapid shifts in population dynamics and lower ecological stability.The theoretical implications and potential applications of these findings warrant further investigation.展开更多
Accurate timing of myelination is crucial for the proper functioning of the central nervous system. Here, we identified a de novo heterozygous mutation in TMEM63A (c.1894G>A;p. Ala632Thr) in a 7-year-old boy exhibi...Accurate timing of myelination is crucial for the proper functioning of the central nervous system. Here, we identified a de novo heterozygous mutation in TMEM63A (c.1894G>A;p. Ala632Thr) in a 7-year-old boy exhibiting hypomyelination. A Ca2+ influx assay suggested that this is a loss-of-function mutation. To explore how TMEM63A deficiency causes hypomyelination, we generated Tmem63a knockout mice. Genetic deletion of TMEM63A resulted in hypomyelination at postnatal day 14 (P14) arising from impaired differentiation of oligodendrocyte precursor cells (OPCs). Notably, the myelin dysplasia was transient, returning to normal levels by P28. Primary cultures of Tmem63a^(−/−) OPCs presented delayed differentiation. Lentivirus-based expression of TMEM63A but not TMEM63A_A632T rescued the differentiation of Tmem63a^(−/−) OPCs in vitro and myelination in Tmem63a^(−/−) mice. These data thus support the conclusion that the mutation in TMEM63A is the pathogenesis of the hypomyelination in the patient. Our study further demonstrated that TMEM63A-mediated Ca^(2+) influx plays critical roles in the early development of myelin and oligodendrocyte differentiation.展开更多
This paper focuses on the problems existing in the teaching of analog electronic technology courses,and puts forward the teaching reform idea of combining marine knowledge with the course content.Based on the BOPPPS t...This paper focuses on the problems existing in the teaching of analog electronic technology courses,and puts forward the teaching reform idea of combining marine knowledge with the course content.Based on the BOPPPS teaching model,specific teaching links are designed,and the teaching practice is carried out as an example of a low-power,high-precision microprocessor development project.The practical results show that the teaching reform improves students’learning interest and participation,cultivates students’engineering practice and innovation abilities,and provides a useful reference for the teaching reform of related courses.展开更多
Epstein-Barr virus(EBV)is very common,with the infection rate in adults over 90%worldwide.Infectious mononucleosis(IM)is caused by primary infection with EBV.Most IM patients are generally considered to have a favorab...Epstein-Barr virus(EBV)is very common,with the infection rate in adults over 90%worldwide.Infectious mononucleosis(IM)is caused by primary infection with EBV.Most IM patients are generally considered to have a favorable prognosis,but a few patients will also develop complications.Children with severe symptoms will require hospitalization.However,the disease burden of children hospitalized with IM in China has been rarely described.In this study,we included the Face sheets of discharge medical records from 27 member children's hospitals of Futang Research Center of Pediatric Development from Jan 1st,2016 to Dec 31st,2020,and medical information such as gender,age,region,time of admission,length of stay and expenditure were extracted.There were 24,120 IM cases,which accounted for 0.42%(24,120/5,693,262)of all hospitalized cases during this period.The ratio of male to female was 1.48:1.Hospitalization for IM in the 4-6 years age group was the highest among inpatients of all age groups.Case numbers increased year by year between 2016 and 2020,and the monthly hospitalization was generally high from Jul to Sep but reduced from Jan to Feb per year.Bronchitis/pneumonia and hepatic dysfunction were two common complications in hospitalized IM patients.The median length of stay was 8 days,and the median cost of hospitalization was 970.59 US dollars.This study will help understand the epidemiological characteristics and disease burden of hospitalized children with IM in China.展开更多
Implantable bioelectronics for analyzing physiological biomarkers has recently been recognized as a promising technique in medical treatment or diagnostics. In this study, we developed a self-powered implantable skinl...Implantable bioelectronics for analyzing physiological biomarkers has recently been recognized as a promising technique in medical treatment or diagnostics. In this study, we developed a self-powered implantable skinlike glucometer for real-time detection of blood glucose level in vivo. Based on the piezo-enzymatic-reaction coupling effect of GOx@ZnO nanowire, the device under an applied deformation can actively output piezoelectric signal containing the glucose-detecting information. No external electricity power source or battery is needed for this device, and the outputting piezoelectric voltage acts as both the biosensing signal and electricity power. A practical application of the skin-like glucometer implanted in mouse body for detecting blood glucose level has been simply demonstrated. These results provide a new technique path for diabetes prophylaxis and treatment.展开更多
High-performance and cost-effective catalysts for water splitting are key components of hydrogen-based energy technologies. Metal-organic framework(MOF)-derived metal phosphide composites have immense potential as hig...High-performance and cost-effective catalysts for water splitting are key components of hydrogen-based energy technologies. Metal-organic framework(MOF)-derived metal phosphide composites have immense potential as highly active and stable electrocatalysts but suffer from the poor efficacy of available electrode assembly methods. In this study, an MOF-derived nitrogen-doped porous carbon/Co/Co P/carbon paper(NC/Co/Co P/CP) composite electrode was assembled by electrophoretic deposition and post-processing reactions. The binder-free electrode showed good catalytic activity, significantly higher than that of traditional electrodes. The electrode required overpotentials of 208 and 350 m V to achieve a current density of 10 m A/cm^2 for the hydrogen and oxygen evolution reactions, respectively. This facile synthetic method provides a promising route for designing metal-doped and multi-metal phase MOF-derived composite electrodes for energy storage and conversion devices.展开更多
The rapid development of flexible electronic devices requires the design of flexible energy-storage devices. Lithium-sulfur(Li-S) batteries are attracting much interest due to their high energy density. Therefore, fle...The rapid development of flexible electronic devices requires the design of flexible energy-storage devices. Lithium-sulfur(Li-S) batteries are attracting much interest due to their high energy density. Therefore, flexible Li-S batteries with high areal capacity are desired. Herein, we fabricated freestanding reduced graphene oxide-sulfur(RGO@S) composite films with a cross-linked structure using a blade coating technique, followed by a subsequent chemical reduction. The porous cross-linked structure endows the composite films with excellent electrochemical performance. The batteries based on RGO@S composite films could exhibit a high discharge capacity of 1381 m Ah/g at 0.1 C and excellent cycle stability. Furthermore, the freestanding composite film possesses excellent conductivity and high mechanical strength. Therefore, they can be used as the cathodes of flexible Li-S batteries. As a proof of concept, soft-packaged Li-S batteries were assembled and remained stable electrochemical performance under different bending states.展开更多
Heading Chinese cabbage(Brassica rapa L.syn.B.campestris L.ssp.chinensis Makino var.pekinensis(Rupr.)J.Cao et Sh.Cao)is a cruciferous Brassica vegetable that has a triplicate genome,owing to an ancient genome duplicat...Heading Chinese cabbage(Brassica rapa L.syn.B.campestris L.ssp.chinensis Makino var.pekinensis(Rupr.)J.Cao et Sh.Cao)is a cruciferous Brassica vegetable that has a triplicate genome,owing to an ancient genome duplication event.It is unclear whether the duplicated homologs have conserved or diversi fied functions.Hydrogen sulfide(H_(2)S)is a plant gasotransmitter that plays important physiological roles in growth,development,and responses to environmental stresses.The modification of cysteines through S-sulfhydration is an important mechanism of H_(2)S,which regulates protein functions.H?S promotes flowering in Arabidopsis and heading Chinese cabbage.Here we investigated the molecular mechanisms of H_(2)S used to promote flowering in the latter.Four,five,and four BraFLC,BraSOC I,and BraFT homologs were identi fi ed in heading Chinese cabbage.Different BraFLC proteins were bound to different CArG boxes in the promoter regions of the BraSOC I and BraFT homologs,producing different binding patterns.Thus,there may be functionally diverse BraFLC homologs in heading Chinese cabbage.Exogenous H_(2)S at 100μmol L^(-1) significantly promoted flowering by compensating for insuf fi cient vernalization.BraFLC 1 and BraFLC_(3) underwent S-sulfhydration by H_(2)S,after which their abilities to bind most BraSOC I or BraFT promoter probes weakened or even disappeared.These changes in binding ability were consistent with the expression pattern of the BraFT and BraSOC I homologs in seedlings treated with H_(2)S.These results indicated that H_(2)S signaling regulates flowering time.In summary,H_(2)S signaling promoted plant flowering by weakening or eliminating the binding abilities of BraFLCs to downstream promoters through S-sulfhydration.展开更多
Remifentanil is widely used to control intraoperative pain. However, its analgesic effect is limited by the generation of postoperative hyperalgesia. In this study, we investigated whether the impairment of transmembr...Remifentanil is widely used to control intraoperative pain. However, its analgesic effect is limited by the generation of postoperative hyperalgesia. In this study, we investigated whether the impairment of transmembrane protein 16C(TMEM16C)/Slack is required for a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic receptor(AMPAR) activation in remifentanil-induced postoperative hyperalgesia. Remifentanil anesthesia reduced the paw withdrawal threshold from 2 h to 48 h postoperatively,with a decrease in the expression of TMEM16C and Slack in the dorsal root ganglia(DRG) and spinal cord.Knockdown of TMEM16C in the DRG reduced the expression of Slack and elevated the basal peripheral sensitivity and AMPAR expression and function. Overexpression of TMEM16C in the DRG impaired remifentanilinduced ERK1/2 phosphorylation and behavioral hyperalgesia. AMPAR-mediated current and neuronal excitability were downregulated by TMEM16C overexpression in the spinal cord. Taken together, these findings suggest that TMEM16C/Slack regulation of excitatory synaptic plasticity via GluA1-containing AMPARs is critical in the pathogenesis of remifentanil-induced postoperative hyperalgesia in rats.展开更多
基金Supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB42000000)the National Natural Science Foundation of China(No.42376092)the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(No.2022QNLM030004)。
文摘Nereididae is a prolific annelid family widely distributed in the world oceans,especially in the Indo-Pacific Convergence Zone(IPCZ).However,its biogeographic pattern remains unexplored in IPCZ.To contribute to the understanding of biodiversity and biogeography of Nereididae in the IPCZ,we integrated historical data of species distributions with those of model-predicted ones to determine the biogeographic patterns of nereid species,from which we projected to its future distribution patterns for 2090-2100 under different climate scenarios(SSP1-1.9 and SSP5-8.5).Functional diversity within IPCZ was assessed using functional richness,functional evenness,and functional disparity.Divergence times within Nereididae were estimated using three DNA marker genes(COI,16S,and 18S rRNA),and a time tree was constructed based on a strict molecular clock model.The IPCZ was established as a key Nereididae biodiversity hotspot through distribution modelling of 256 species(44 genera),and temperature emerging as the predominant climatic driver of species distribution patterns.The distribution of species and functional diversity is notable for its non-centralized pattern.We projected that by the end of the century,areas of medium-to-high species richness will expand significantly under the low-emission SSP1-1.9 climate scenario.However,under the high-emission SSP5-8.5 scenario,the suitability of these regions significantly declines,posing an increasingly severe threat to biodiversity.In addition,by molecular clock analysis,we revealed that the evolutionary divergence of extant nereidid species occurred mainly in the Cretaceous and Jurassic,suggesting that paleogeographical and environmental events,such as oceanic anoxic events,might have played a pivotal role in shaping the evolutionary trajectory and ecological adaptations of marine annelids.These findings highlight the importance of considering both current biodiversity patterns and historical contexts in conservation planning,and provided insights into the potential factors on the biogeographic distribution and evolutionary processes of Nereididae.
基金Supported by the Key Research and Development Program of Shandong(No.2022LZGC015)the National Key R&D Program of China(No.2022YFD2401400)+1 种基金the Taishan Scholars Program,the National Key R&D Program of China(No.2022YFD2400304)the Agricultural Seed Project of Shandong Key R&D Program(No.2024LZGCQY003)。
文摘Atmospheric carbon dioxide(CO_(2))levels are escalating at an unprecedented rate,leading to the phenomenon of ocean acidification(OA).Parental exposure to acidification has the potential to enhance offspring resilience through cross-generation plasticity.In this study,we analyzed larval growth and transcriptomic profiles in the Pacific oyster,Crassostrea gigas,a species of significant ecological relevance,under both control and elevated CO_(2)conditions experienced by their parental generation.Our findings indicate that the oyster populations exposed to OA exhibited a higher incidence of abnormalities during the D-shaped larval stage,followed by accelerated growth at the eyed stage.Through a comparative transcriptomic investigation of eyed larvae(25 d after fertilization),we observed that parental exposure to OA substantially influenced the gene expression in the offspring.Genes associated with lipid catabolism and shell formation were notably upregulated in oysters with parental OA exposure,potentially playing a role in cross-generational conditioning and conferring resilience to OA stressors.These results underscore the profound impact of OA on oyster larval development via cross-generational mechanisms and shed light on the molecular underpinnings of cross-generation plasticity.
基金supported in part by National key R&D projects(2024YFB4207203)National Natural Science Foundation of China(52401376)+3 种基金the Zhejiang Provincial Natural Science Foundation of China under Grant(No.LTGG24F030004)Hangzhou Key Scientific Research Plan Project(2024SZD1A24)“Pioneer”and“Leading Goose”R&DProgramof Zhejiang(2024C03254,2023C03154)Jiangxi Provincial Gan-Po Elite Support Program(Major Academic and Technical Leaders Cultivation Project,20243BCE51180).
文摘Recently,Internet ofThings(IoT)has been increasingly integrated into the automotive sector,enabling the development of diverse applications such as the Internet of Vehicles(IoV)and intelligent connected vehicles.Leveraging IoVtechnologies,operational data fromcore vehicle components can be collected and analyzed to construct fault diagnosis models,thereby enhancing vehicle safety.However,automakers often struggle to acquire sufficient fault data to support effective model training.To address this challenge,a robust and efficient federated learning method(REFL)is constructed for machinery fault diagnosis in collaborative IoV,which can organize multiple companies to collaboratively develop a comprehensive fault diagnosis model while keeping their data locally.In the REFL,the gradient-based adversary algorithm is first introduced to the fault diagnosis field to enhance the deep learning model robustness.Moreover,the adaptive gradient processing process is designed to improve the model training speed and ensure the model accuracy under unbalance data scenarios.The proposed REFL is evaluated on non-independent and identically distributed(non-IID)real-world machinery fault dataset.Experiment results demonstrate that the REFL can achieve better performance than traditional learning methods and are promising for real industrial fault diagnosis.
基金supported by the National Natural Science Foundation of China(Nos.42277315,22066009)the Scientific Research Startup Fund of Hainan University(Nos.XJ2300005916,kyqd(zr)22185)+1 种基金supported by Scientific Research Project of Hainan Higher Education Institutions(No.Hnky2023-9)Innovational Fund for Scientific and Technological Personnel of Hainan Province(No.KJRC2023C12)。
文摘Synergy strategy of photocatalysts and polymer resins are promising technology for marine antifouling.However,it is still a main challenge to obtain a green,safe,and efficient antifouling coatings.Herein,carbon(graphene or CNT)modified Ti O_(2)photocatalyst was synthesized via hydrothermal and annealing process and has successfully applied in acrylate fluoroboron polymer(ABFP)composite coating.Morphology and chemical composition were detailed characterized.The graphene or CNT acted as a bridge with supplemental spatial structures(petal gaps,entanglement)and new functional groups(C-O,C-Ti-O,etc.)on Ti O_(2)particle.Carbon nanotube(CNT)modified TiO_(2)-ABFP coatings(BTCP)achieved excellent antibacterial and anti-diatom adhesion rate of 89.3%-96.70%and 99.00%-99.50%,which was 1.84-4.94-fold more than that of the single ABFP.CNT or graphene served as electronic bridges was considered as the crucial mechanism,which significantly improved the light absorption range and capacity,conductivity,and photoelectric response of Ti O_(2),and further accelerated the generation and transfer of free radicals to the surface of BTCP or FTGP.Moreover,the improvement of catalyst activity synergizes with the smooth surface,hydrophilicity,and slow hydrolysis of composite coatings,achieved long-term and efficient antifouling performance.This work provides a new insight into the modification of Ti O_(2)and antifouling mechanism of polymer coating.
文摘Objective: To study the mechanism of Sijunzi decoction treating limb weakness in spleen Qi deficiency (SQD) based on the myonuclear domain (MND) theory. Methods: 40 male Sprague-Dawley rats were randomly divided into the normal group, SQD model group (model group), SQD+ still water group (SW group) and SQD+ Sijunzi decoction group (CM group), 10 rats each group;Grip-Strength Meter was used to measure limb grip strength;transmission electron microscope was employed to observe the ultrastructural changes of the myofibers, Image Pro 6.0 was used to measure the myonuclear numbers, cross-section area (CSA) and then their ratios (the MND sizes) were calculated, immunofluorescence assay was chosen to test the expressions of paired box gene 7 (Pax7) and myogenic differentiation antigen (MyoD). Results: Compared with those in the normal group, limb grip strength was decreased, sarcomeres were abnormal, and all the myonuclear numbers, CSA and MND sizes were reduced, but the Pax7+ cell numbers were increased, significantly, in the model and SW groups;Compared with those in the model and SW groups, limb grip strength was increased, sarcomeres were basically normal, the myonuclear number and CSA were both greater, and the Pax7+ and MyoD+ cell numbers were both increased, significantly, in the CM group. Conclusion: Sijunzi decoction might increase the myonuclear number by activating the MSCs to treat limb weakness in SQD.
文摘株型是影响谷类作物产量的重要性状,株型改良对提高作物产量具有重要意义。独脚金内酯(strigolactones,SLs)作为一种最新被鉴定的植物激素,其通过抑制腋芽的伸长调控分枝/分蘖的形成。β-胡萝卜素异构酶(D27s)是SLs合成途径的关键酶,通过对谷子(Setaria italica)β-胡萝卜素异构酶典型结构域Pfam:DUF4033进行分析,鉴定到3个谷子D27s基因家族成员(Seita.8G168400、Seita.6G088800和Seita.3G050900)。蛋白质特性分析显示,谷子D27s蛋白由271–277个氨基酸残基组成,分子量为30.1–30.4 k Da,等电点为5.85–9.31,不稳定系数介于38.48–74.47之间,且均定位于叶绿体;系统进化分析发现,谷子D27s家族成员位于3个不同进化分支;顺式作用元件预测显示,SiD27-1(Seita.8G168400)可能参与调控生物节律、生长素介导的生长发育以及干旱和低温等胁迫应答过程。基因表达分析显示,SiD27-1在谷子多分蘖材料中表达下调,在低磷胁迫处理下,D27s基因均能产生不同程度的响应,并且Si D27-1的响应较其它成员更快速。单倍型分析结果表明,SiD27-1的H001单倍型为优异单倍型,对谷子的株高、抽穗期和产量改良具有重要应用价值。综上,推测SiD27-1极可能在SLs合成中发挥关键作用并对谷子株型产生影响。研究结果为深入揭示D27s对谷子分蘖形成的调控机制奠定了基础,也为谷子株型分子设计育种提供了优异的等位变异位点。
基金The National Natural Science Foundation of China under contract Nos 42206126 and 42076044the Natural Science Foundation of Shandong Province under contract No.ZR2021QD099.
文摘Plate culture counting and strain isolation methods were utilized to assess the species richness and abundance of planktonic and attached bacteria on glass plates in the surface and bottom seawater of Qingdao Middle Harbor over a year,with monthly and quarterly sampling.Both species richness and bacterial numbers exhibited seasonal variations.Specifically,the abundance of attached bacteria and bacterioplankton peaked in June and July,corresponding to higher water temperatures in summer and autumn,while lower abundances were noted in January and December during cooler periods.Throughout the year,the species richness of attached bacteria consistently exceeded that of planktonic bacteria in both shallow and deep waters.Pseudoalteromonas emerged as the most prevalent genus among both planktonic and attached bacteria in surface and bottom seawater samples.Furthermore,the magnitude of changes in species richness and abundance for attached bacteria(0.66×10^(5)-15.85×10^(5)CFU/cm^(2))was greater than that observed for planktonic bacteria(0.58×10^(8)-5.33×10^(8)CFU/L).We propose that the attached bacterial populations,situated in limited microenvironments within the larger seawater ecosystem,exhibit heightened sensitivity to environmental fluctuations,resulting in more rapid shifts in population dynamics and lower ecological stability.The theoretical implications and potential applications of these findings warrant further investigation.
基金supported by grants from the National Key R&D Program of China(2019YFA0801603)the Guangdong High Level Innovation Research Institute(2021B0909050004)+2 种基金the National Natural Science Foundation of China(32330044,32170951,82201615,and 82101393)the Natural Science Foundation of Jiangsu Province(BK20201255 and BK20210008)the Fundamental Research Funds for the Central Universities(021414380533).
文摘Accurate timing of myelination is crucial for the proper functioning of the central nervous system. Here, we identified a de novo heterozygous mutation in TMEM63A (c.1894G>A;p. Ala632Thr) in a 7-year-old boy exhibiting hypomyelination. A Ca2+ influx assay suggested that this is a loss-of-function mutation. To explore how TMEM63A deficiency causes hypomyelination, we generated Tmem63a knockout mice. Genetic deletion of TMEM63A resulted in hypomyelination at postnatal day 14 (P14) arising from impaired differentiation of oligodendrocyte precursor cells (OPCs). Notably, the myelin dysplasia was transient, returning to normal levels by P28. Primary cultures of Tmem63a^(−/−) OPCs presented delayed differentiation. Lentivirus-based expression of TMEM63A but not TMEM63A_A632T rescued the differentiation of Tmem63a^(−/−) OPCs in vitro and myelination in Tmem63a^(−/−) mice. These data thus support the conclusion that the mutation in TMEM63A is the pathogenesis of the hypomyelination in the patient. Our study further demonstrated that TMEM63A-mediated Ca^(2+) influx plays critical roles in the early development of myelin and oligodendrocyte differentiation.
基金Education Reform Research Project of Hainan Tropical Ocean University(RHYjg2025-10)Key Research and Development Projects of Hainan Province,China(ZDYF2024GXJS303)。
文摘This paper focuses on the problems existing in the teaching of analog electronic technology courses,and puts forward the teaching reform idea of combining marine knowledge with the course content.Based on the BOPPPS teaching model,specific teaching links are designed,and the teaching practice is carried out as an example of a low-power,high-precision microprocessor development project.The practical results show that the teaching reform improves students’learning interest and participation,cultivates students’engineering practice and innovation abilities,and provides a useful reference for the teaching reform of related courses.
基金supported by the National Natural Science Foundation of China (82002130)supported by the CAMS Innovation Fund for Medical Sciences (2019-I2M-5-026)
文摘Epstein-Barr virus(EBV)is very common,with the infection rate in adults over 90%worldwide.Infectious mononucleosis(IM)is caused by primary infection with EBV.Most IM patients are generally considered to have a favorable prognosis,but a few patients will also develop complications.Children with severe symptoms will require hospitalization.However,the disease burden of children hospitalized with IM in China has been rarely described.In this study,we included the Face sheets of discharge medical records from 27 member children's hospitals of Futang Research Center of Pediatric Development from Jan 1st,2016 to Dec 31st,2020,and medical information such as gender,age,region,time of admission,length of stay and expenditure were extracted.There were 24,120 IM cases,which accounted for 0.42%(24,120/5,693,262)of all hospitalized cases during this period.The ratio of male to female was 1.48:1.Hospitalization for IM in the 4-6 years age group was the highest among inpatients of all age groups.Case numbers increased year by year between 2016 and 2020,and the monthly hospitalization was generally high from Jul to Sep but reduced from Jan to Feb per year.Bronchitis/pneumonia and hepatic dysfunction were two common complications in hospitalized IM patients.The median length of stay was 8 days,and the median cost of hospitalization was 970.59 US dollars.This study will help understand the epidemiological characteristics and disease burden of hospitalized children with IM in China.
基金supported by the National Natural Science Foundation of China (11674048)the Fundamental Research Funds for the Central Universities (N160502002)Liaoning BaiQianWan Talents Program (2014921017)
文摘Implantable bioelectronics for analyzing physiological biomarkers has recently been recognized as a promising technique in medical treatment or diagnostics. In this study, we developed a self-powered implantable skinlike glucometer for real-time detection of blood glucose level in vivo. Based on the piezo-enzymatic-reaction coupling effect of GOx@ZnO nanowire, the device under an applied deformation can actively output piezoelectric signal containing the glucose-detecting information. No external electricity power source or battery is needed for this device, and the outputting piezoelectric voltage acts as both the biosensing signal and electricity power. A practical application of the skin-like glucometer implanted in mouse body for detecting blood glucose level has been simply demonstrated. These results provide a new technique path for diabetes prophylaxis and treatment.
基金supported by the National Natural Science Foundation of China(21573033)Shandong Provincial Natural Science Foundation,China(ZR2018BB037)+1 种基金Project of Shandong Province Higher Educational Science and Technology Program(J17KA104)Project of Qingdao Applied Basic Research Programs of Science and Technology(18-2-2-10-jch and 18-2-2-35-jch)~~
文摘High-performance and cost-effective catalysts for water splitting are key components of hydrogen-based energy technologies. Metal-organic framework(MOF)-derived metal phosphide composites have immense potential as highly active and stable electrocatalysts but suffer from the poor efficacy of available electrode assembly methods. In this study, an MOF-derived nitrogen-doped porous carbon/Co/Co P/carbon paper(NC/Co/Co P/CP) composite electrode was assembled by electrophoretic deposition and post-processing reactions. The binder-free electrode showed good catalytic activity, significantly higher than that of traditional electrodes. The electrode required overpotentials of 208 and 350 m V to achieve a current density of 10 m A/cm^2 for the hydrogen and oxygen evolution reactions, respectively. This facile synthetic method provides a promising route for designing metal-doped and multi-metal phase MOF-derived composite electrodes for energy storage and conversion devices.
基金supported by the National Natural Science Foundation of China(21573116 , 51822205 , 21875121 and 51602218)Ministry of Science and Technology of China(2017YFA0206701)+1 种基金Ministry of Education of China(B12015)the Young Thousand Talents Program
文摘The rapid development of flexible electronic devices requires the design of flexible energy-storage devices. Lithium-sulfur(Li-S) batteries are attracting much interest due to their high energy density. Therefore, flexible Li-S batteries with high areal capacity are desired. Herein, we fabricated freestanding reduced graphene oxide-sulfur(RGO@S) composite films with a cross-linked structure using a blade coating technique, followed by a subsequent chemical reduction. The porous cross-linked structure endows the composite films with excellent electrochemical performance. The batteries based on RGO@S composite films could exhibit a high discharge capacity of 1381 m Ah/g at 0.1 C and excellent cycle stability. Furthermore, the freestanding composite film possesses excellent conductivity and high mechanical strength. Therefore, they can be used as the cathodes of flexible Li-S batteries. As a proof of concept, soft-packaged Li-S batteries were assembled and remained stable electrochemical performance under different bending states.
基金the National Natural Science Foundation of China(31972428 and 31672140)the Shanxi Province Natural Science Foundation(201801D121191 and 201801D121197).
文摘Heading Chinese cabbage(Brassica rapa L.syn.B.campestris L.ssp.chinensis Makino var.pekinensis(Rupr.)J.Cao et Sh.Cao)is a cruciferous Brassica vegetable that has a triplicate genome,owing to an ancient genome duplication event.It is unclear whether the duplicated homologs have conserved or diversi fied functions.Hydrogen sulfide(H_(2)S)is a plant gasotransmitter that plays important physiological roles in growth,development,and responses to environmental stresses.The modification of cysteines through S-sulfhydration is an important mechanism of H_(2)S,which regulates protein functions.H?S promotes flowering in Arabidopsis and heading Chinese cabbage.Here we investigated the molecular mechanisms of H_(2)S used to promote flowering in the latter.Four,five,and four BraFLC,BraSOC I,and BraFT homologs were identi fi ed in heading Chinese cabbage.Different BraFLC proteins were bound to different CArG boxes in the promoter regions of the BraSOC I and BraFT homologs,producing different binding patterns.Thus,there may be functionally diverse BraFLC homologs in heading Chinese cabbage.Exogenous H_(2)S at 100μmol L^(-1) significantly promoted flowering by compensating for insuf fi cient vernalization.BraFLC 1 and BraFLC_(3) underwent S-sulfhydration by H_(2)S,after which their abilities to bind most BraSOC I or BraFT promoter probes weakened or even disappeared.These changes in binding ability were consistent with the expression pattern of the BraFT and BraSOC I homologs in seedlings treated with H_(2)S.These results indicated that H_(2)S signaling regulates flowering time.In summary,H_(2)S signaling promoted plant flowering by weakening or eliminating the binding abilities of BraFLCs to downstream promoters through S-sulfhydration.
基金supported by the National Natural Science Foundation of China (82071243, 81801107, 81772043, and 81400908)Tianjin Natural Science Foundation (20JCYBJC00460)Young Elite Scientists Sponsorship Program by Tianjin Municipality, China (TJSQNTJ-2020-10)。
文摘Remifentanil is widely used to control intraoperative pain. However, its analgesic effect is limited by the generation of postoperative hyperalgesia. In this study, we investigated whether the impairment of transmembrane protein 16C(TMEM16C)/Slack is required for a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic receptor(AMPAR) activation in remifentanil-induced postoperative hyperalgesia. Remifentanil anesthesia reduced the paw withdrawal threshold from 2 h to 48 h postoperatively,with a decrease in the expression of TMEM16C and Slack in the dorsal root ganglia(DRG) and spinal cord.Knockdown of TMEM16C in the DRG reduced the expression of Slack and elevated the basal peripheral sensitivity and AMPAR expression and function. Overexpression of TMEM16C in the DRG impaired remifentanilinduced ERK1/2 phosphorylation and behavioral hyperalgesia. AMPAR-mediated current and neuronal excitability were downregulated by TMEM16C overexpression in the spinal cord. Taken together, these findings suggest that TMEM16C/Slack regulation of excitatory synaptic plasticity via GluA1-containing AMPARs is critical in the pathogenesis of remifentanil-induced postoperative hyperalgesia in rats.
