Antifungal resistance is the leading cause of antifungal treatment failure in invasive candidiasis.Metabolic rewiring could become a new insight to account for antifungal resistance as to find innovative clinical ther...Antifungal resistance is the leading cause of antifungal treatment failure in invasive candidiasis.Metabolic rewiring could become a new insight to account for antifungal resistance as to find innovative clinical therapies.Here,we show that dynamic surface-enhanced Raman spectroscopy is a promising tool to identify the metabolic differences between fluconazole(Diflucan)-resistant and fluconazole(Diflucan)-sensitive Candida albicans through the signatures of biochemical components and complemented with machine learning algorithms and two-dimensional correlation spectroscopy,an underlying resistance mechanism,that is,the change of purine metabolites induced the resistance of Candida albicans has been clarified yet never reported anywhere.We hope the integrated methodology introduced in this work could be beneficial for the interpretation of cellular regulation,propelling the development of targeted antifungal therapies and diagnostic tools for more efficient management of severe antifungal resistance.展开更多
Polymyxin B,which is a last-line antibiotic for extensively drug-resistant Gram-negative bacterial infections,became available in China in Dec.2017.As dose adjustments are based solely on clinical experience of risk t...Polymyxin B,which is a last-line antibiotic for extensively drug-resistant Gram-negative bacterial infections,became available in China in Dec.2017.As dose adjustments are based solely on clinical experience of risk toxicity,treatment failure,and emergence of resistance,there is an urgent clinical need to perform therapeutic drug monitoring(TDM)to optimize the use of polymyxin B.It is thus necessary to standardize operating procedures to ensure the accuracy of TDM and provide evidence for their rational use.We report a consensus on TDM guidelines for polymyxin B,as endorsed by the Infection and Chemotherapy Committee of the Shanghai Medical Association and the Therapeutic Drug Monitoring Committee of the Chinese Pharmacological Society.The consensus panel was composed of clinicians,pharmacists,and microbiologists from different provinces in China and Australia who made recommendations regarding target concentrations,sample collection,reporting,and explanation of TDM results.The guidelines provide the first-ever consensus on conducting TDM of polymyxin B,and are intended to guide optimal clinical use.展开更多
A dual-frequency(105/140 GHz)MW-level continuous-wave gyrotron was developed for fusion application at Institute of Applied Electronics,China Academy of Engineering Physics.This gyrotron employs a cylindrical cavity w...A dual-frequency(105/140 GHz)MW-level continuous-wave gyrotron was developed for fusion application at Institute of Applied Electronics,China Academy of Engineering Physics.This gyrotron employs a cylindrical cavity working in the TE18,7 mode at 105 GHz and the TE24,9 mode at 140 GHz.A triode magnetron injection gun and a built-in quasi-optical mode converter were designed to operate at these two frequencies.For the proof-test phase,the gyrotron was equipped with a single-disk boron nitride window to achieve radio frequency output with a power of~500 k W for a short-pulse duration.In the preliminary short-pulse proof-test in the first quarter of2021,the dual-frequency gyrotron achieved output powers of 300 k W at 105 GHz and 540 k W at140 GHz,respectively,under 5 Hz 1 ms continuous pulse-burst operations.Power upgrade and pulse-width extension were hampered by the limitation of the high-voltage power supply and output window.This gyrotron design was preliminarily validated.展开更多
Nickel-rich layered oxide LiNi_(1-x-y)Co_(x)Al_yO_(2)(NCA) with high theoretical capacity is a promising cathode material for the next-generation high-energy batteries.However,it undergoes a rapid capacity fading when...Nickel-rich layered oxide LiNi_(1-x-y)Co_(x)Al_yO_(2)(NCA) with high theoretical capacity is a promising cathode material for the next-generation high-energy batteries.However,it undergoes a rapid capacity fading when operating at high temperature due to the accelerated cathode/electrolyte interfacial reactions and adhesive efficacy loss of conventional polyvinylideneffuoride(PVdF) binder.Herein,poly(acrylonitrile-co-methyl acrylate) copolymer is designed with electron-rich-C≡N groups as a novel binder for LiNi_(0.8)Co_(0.1)Al_(0.1)O_(2) cathode at high temperature.The electron-rich-C≡N groups are able to coordinate with the active Ni^(3+) on the surface of NCA,alleviating electrolyte decomposition and cathode structure degradation.Moreover,the strong adhesive ability is conducive to maintain integrity of electrodes upon cycling at 55℃.In consequence,the NCA electrodes with this functional binder display improved cycling stability(81.5% capacity retention after 100 cycles) and rate performance at 55℃.展开更多
A high-power 28 GHz gyrotron has been successfully developed at the Institute of Applied Electronics,China Academy of Engineering Physics.This gyrotron was designed for electron cyclotron resonance heating(ECRH)in the...A high-power 28 GHz gyrotron has been successfully developed at the Institute of Applied Electronics,China Academy of Engineering Physics.This gyrotron was designed for electron cyclotron resonance heating(ECRH)in the spherical tokamak XL-50.A diode magnetron injection gun was designed to produce the required gyrating electron beam.The gyrotron operates in the TE8,3mode in a cylindrical open cavity.An internal quasi-optical mode converter was designed to convert the operating mode into a fundamental Gaussian wave beam and separate the spent electron beam from the outgoing microwave power.A tube has been built and successfully tested.The operational frequency of the tube is 28.1 GHz.For beam parameters at an accelerating voltage of 71 kV and beam current of 16 A,the gyrotron has delivered an output power of 400 kW,with a pulse length of 5 s.The output efficiency is about 50%with a singlestage depressed collector.The gyrotron has been installed on the XL-50 and has played an important role in the ECRH experiments.展开更多
In order to achieve the transformation from the conventional soil-covering cultivation of Tricholoma giganteum with bags to the soilless cultivation with bottles and understand the mechanism of primordium formation of...In order to achieve the transformation from the conventional soil-covering cultivation of Tricholoma giganteum with bags to the soilless cultivation with bottles and understand the mechanism of primordium formation of Tricholoma giganteum,with Tricholoma giganteum mycelia as experimental materials,this paper studied the effect of different fruiting treatments on the activity of three enzymes in different time.The results showed that from the mycelial recovery to primardial formation and budding under three treatment groups which could form primordium,the tyrosinase activity was relatively stable,and under two treatment groups which could not form primordium,the tyrosinase activity dropped after the rise and reached a maximum on the 9 th day,significantly higher than under the other three groups,indicating that too high tyrosinase inhibited primardial formation; the prolease and amylase activity was effectively activated before primardial formation,and the enzymic activity was significantly higher than under the two treatment groups which could not form primordium.展开更多
Refractory lithospheric deep roots are the cornerstone for the prolonged stability of cratons and mantle xenoliths are normally the key targets for study on the evolution of such deep roots. In regions with few mantle...Refractory lithospheric deep roots are the cornerstone for the prolonged stability of cratons and mantle xenoliths are normally the key targets for study on the evolution of such deep roots. In regions with few mantle xenoliths, the basalts enriched in radiogenic isotopic compositions due to marked lithospheric mantle contribution are crucial to unmask the lithospheric mantle evolution based on a comprehensive study involving petrology, geochemistry and thermodynamic modelling. Here, the Early Cretaceous basalts from the northwest North China Craton with few mantle xenoliths are taken as an example to show the significance of enriched basalts on the study of lithospheric mantle. These basalts are characterized by high silica and alkali contents(SiO_(2)=45.8–59.8 wt.%, K_(2)O+Na_(2)O=4.81–9.88 wt.%), arc-type trace-element patterns and enriched radiogenic isotope compositions(e.g., ε_(Nd)=-2.64–-12.88,^(87)Sr/^(86)Sr=0.7063–0.7093). The TiO_(2) and FeO(total) contents are higher than those of natural and experimental melts from refractory mantle peridotite but comparable to those of partial melts of fertile mantle rocks.The high contents of fluid-loving elements(e.g., Rb and Ba) suggest source metasomatism by aqueous fluids. Combined with thermodynamic modelling and regional tectonic history, these enriched basalts likely record simultaneous decompression melting of asthenosphere and low-extent melting of thin and fertile lithospheric mantle fluxed by aqueous fluids from the subducted Paleo-Asian oceanic slab. The newly unmasked lithospheric mantle under the western NCC contrasts with the coeval thick and refractory one supporting the eastern NCC, and highlights that the craton destruction, especially the loss of its ancient refractory mantle root, should take place in a diachronous manner related to the craton-girded subduction episodes. Our study illustrates the potential of enriched basalts to recover the nature and evolution of mantle lithosphere beneath craton margins and associated tectonic histories.展开更多
基金supported by grants from the National Natural Science Foundation of China(Nos.22074015 and 82074428)Youth Talent Cultivation Initiation Fund of Zhongda Hospital,Southeast University(No.CZXM-GSP-RC110)to Hao Li+1 种基金Evidence-Based Capacity Building for TCM Specialty Therapies for Skin Diseases of National Administration of TCMInnovative Team Projects of Shanghai Municipal Commission of Health(No.2022CX011)to Fulun Li.
文摘Antifungal resistance is the leading cause of antifungal treatment failure in invasive candidiasis.Metabolic rewiring could become a new insight to account for antifungal resistance as to find innovative clinical therapies.Here,we show that dynamic surface-enhanced Raman spectroscopy is a promising tool to identify the metabolic differences between fluconazole(Diflucan)-resistant and fluconazole(Diflucan)-sensitive Candida albicans through the signatures of biochemical components and complemented with machine learning algorithms and two-dimensional correlation spectroscopy,an underlying resistance mechanism,that is,the change of purine metabolites induced the resistance of Candida albicans has been clarified yet never reported anywhere.We hope the integrated methodology introduced in this work could be beneficial for the interpretation of cellular regulation,propelling the development of targeted antifungal therapies and diagnostic tools for more efficient management of severe antifungal resistance.
基金the Shanghai Leading Talents Award,Shanghai Municipal Health Commission(No.LJ2016-01)the Clinical Research Plan of Shanghai Hospital Development Center(No.SHDC2022CRW004)。
文摘Polymyxin B,which is a last-line antibiotic for extensively drug-resistant Gram-negative bacterial infections,became available in China in Dec.2017.As dose adjustments are based solely on clinical experience of risk toxicity,treatment failure,and emergence of resistance,there is an urgent clinical need to perform therapeutic drug monitoring(TDM)to optimize the use of polymyxin B.It is thus necessary to standardize operating procedures to ensure the accuracy of TDM and provide evidence for their rational use.We report a consensus on TDM guidelines for polymyxin B,as endorsed by the Infection and Chemotherapy Committee of the Shanghai Medical Association and the Therapeutic Drug Monitoring Committee of the Chinese Pharmacological Society.The consensus panel was composed of clinicians,pharmacists,and microbiologists from different provinces in China and Australia who made recommendations regarding target concentrations,sample collection,reporting,and explanation of TDM results.The guidelines provide the first-ever consensus on conducting TDM of polymyxin B,and are intended to guide optimal clinical use.
基金supported in part by NSAF(No.U1830201)in part by the State Administration of Science,Technology and Industry for Nation Defense of China,Technology Foundation Project(No.JSJL2019212B006)+1 种基金in part by the Academy Innovation Funder(No.CX2020038)in part by the National Defense Basic Scientific Research Program(No.2018212C015)。
文摘A dual-frequency(105/140 GHz)MW-level continuous-wave gyrotron was developed for fusion application at Institute of Applied Electronics,China Academy of Engineering Physics.This gyrotron employs a cylindrical cavity working in the TE18,7 mode at 105 GHz and the TE24,9 mode at 140 GHz.A triode magnetron injection gun and a built-in quasi-optical mode converter were designed to operate at these two frequencies.For the proof-test phase,the gyrotron was equipped with a single-disk boron nitride window to achieve radio frequency output with a power of~500 k W for a short-pulse duration.In the preliminary short-pulse proof-test in the first quarter of2021,the dual-frequency gyrotron achieved output powers of 300 k W at 105 GHz and 540 k W at140 GHz,respectively,under 5 Hz 1 ms continuous pulse-burst operations.Power upgrade and pulse-width extension were hampered by the limitation of the high-voltage power supply and output window.This gyrotron design was preliminarily validated.
基金supported by the National Natural Science Foundation of China (No. 21875181)the Natural Science Basic Research Program of Shaanxi (Program No. 2019JLP-13)+1 种基金the Shaanxi Key Research and Development Project (No. 2019TSLGY07-05)the 111 Project 2.0 (BP2018008)。
文摘Nickel-rich layered oxide LiNi_(1-x-y)Co_(x)Al_yO_(2)(NCA) with high theoretical capacity is a promising cathode material for the next-generation high-energy batteries.However,it undergoes a rapid capacity fading when operating at high temperature due to the accelerated cathode/electrolyte interfacial reactions and adhesive efficacy loss of conventional polyvinylideneffuoride(PVdF) binder.Herein,poly(acrylonitrile-co-methyl acrylate) copolymer is designed with electron-rich-C≡N groups as a novel binder for LiNi_(0.8)Co_(0.1)Al_(0.1)O_(2) cathode at high temperature.The electron-rich-C≡N groups are able to coordinate with the active Ni^(3+) on the surface of NCA,alleviating electrolyte decomposition and cathode structure degradation.Moreover,the strong adhesive ability is conducive to maintain integrity of electrodes upon cycling at 55℃.In consequence,the NCA electrodes with this functional binder display improved cycling stability(81.5% capacity retention after 100 cycles) and rate performance at 55℃.
基金partially supported by National Natural Science Foundation(No.12175217)the State Administration of Science,Technology and Industry for Nation Defense of China,Technology Foundation Project(No.JSJL2021212B003)。
文摘A high-power 28 GHz gyrotron has been successfully developed at the Institute of Applied Electronics,China Academy of Engineering Physics.This gyrotron was designed for electron cyclotron resonance heating(ECRH)in the spherical tokamak XL-50.A diode magnetron injection gun was designed to produce the required gyrating electron beam.The gyrotron operates in the TE8,3mode in a cylindrical open cavity.An internal quasi-optical mode converter was designed to convert the operating mode into a fundamental Gaussian wave beam and separate the spent electron beam from the outgoing microwave power.A tube has been built and successfully tested.The operational frequency of the tube is 28.1 GHz.For beam parameters at an accelerating voltage of 71 kV and beam current of 16 A,the gyrotron has delivered an output power of 400 kW,with a pulse length of 5 s.The output efficiency is about 50%with a singlestage depressed collector.The gyrotron has been installed on the XL-50 and has played an important role in the ECRH experiments.
文摘In order to achieve the transformation from the conventional soil-covering cultivation of Tricholoma giganteum with bags to the soilless cultivation with bottles and understand the mechanism of primordium formation of Tricholoma giganteum,with Tricholoma giganteum mycelia as experimental materials,this paper studied the effect of different fruiting treatments on the activity of three enzymes in different time.The results showed that from the mycelial recovery to primardial formation and budding under three treatment groups which could form primordium,the tyrosinase activity was relatively stable,and under two treatment groups which could not form primordium,the tyrosinase activity dropped after the rise and reached a maximum on the 9 th day,significantly higher than under the other three groups,indicating that too high tyrosinase inhibited primardial formation; the prolease and amylase activity was effectively activated before primardial formation,and the enzymic activity was significantly higher than under the two treatment groups which could not form primordium.
基金supported by the National Natural Science Foundation of China (Grant Nos.42272053 and 41930215)the MOST Special Fund from the State Key Laboratory of Geological Processes and Mineral Resources (Grant No.MSFGPMR2022-6)。
文摘Refractory lithospheric deep roots are the cornerstone for the prolonged stability of cratons and mantle xenoliths are normally the key targets for study on the evolution of such deep roots. In regions with few mantle xenoliths, the basalts enriched in radiogenic isotopic compositions due to marked lithospheric mantle contribution are crucial to unmask the lithospheric mantle evolution based on a comprehensive study involving petrology, geochemistry and thermodynamic modelling. Here, the Early Cretaceous basalts from the northwest North China Craton with few mantle xenoliths are taken as an example to show the significance of enriched basalts on the study of lithospheric mantle. These basalts are characterized by high silica and alkali contents(SiO_(2)=45.8–59.8 wt.%, K_(2)O+Na_(2)O=4.81–9.88 wt.%), arc-type trace-element patterns and enriched radiogenic isotope compositions(e.g., ε_(Nd)=-2.64–-12.88,^(87)Sr/^(86)Sr=0.7063–0.7093). The TiO_(2) and FeO(total) contents are higher than those of natural and experimental melts from refractory mantle peridotite but comparable to those of partial melts of fertile mantle rocks.The high contents of fluid-loving elements(e.g., Rb and Ba) suggest source metasomatism by aqueous fluids. Combined with thermodynamic modelling and regional tectonic history, these enriched basalts likely record simultaneous decompression melting of asthenosphere and low-extent melting of thin and fertile lithospheric mantle fluxed by aqueous fluids from the subducted Paleo-Asian oceanic slab. The newly unmasked lithospheric mantle under the western NCC contrasts with the coeval thick and refractory one supporting the eastern NCC, and highlights that the craton destruction, especially the loss of its ancient refractory mantle root, should take place in a diachronous manner related to the craton-girded subduction episodes. Our study illustrates the potential of enriched basalts to recover the nature and evolution of mantle lithosphere beneath craton margins and associated tectonic histories.
