Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segment...Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However.展开更多
The rarity of Francisella novicida infection in humans is well-known,and the F.novicida cases occur in immunocompromised patients or those with underlying health problems.Herein,we report the case of a patient with lo...The rarity of Francisella novicida infection in humans is well-known,and the F.novicida cases occur in immunocompromised patients or those with underlying health problems.Herein,we report the case of a patient with long-term diabetes who died following F.novicida infection that caused multiple organ failure,although F.novicida was effectively eliminated using antimicrobial therapy.Microbiological confirmation of F.novicida infection relies on metagenomic next-generation sequencing(mNGS)and pdpD-2 gene-specific identification.This study highlights the importance of early pathogen diagnosis in severely infected patients,particularly in cases of F.novicida,and indicates that mNGS is a useful tool for early diagnosis.展开更多
The increase in emerging and reemerging infectious diseases has underscored the need for the prompt monitoring of intact infectious viruses and the quick assessment of their infectivity.However,molecular techniques ca...The increase in emerging and reemerging infectious diseases has underscored the need for the prompt monitoring of intact infectious viruses and the quick assessment of their infectivity.However,molecular techniques cannot distinguish between intact infectious and noninfectious viruses.Here,two distinct methodologies have been developed for the expeditious and dependable quantification of intact infectious H1N1 virus,and several experiments have been conducted to substantiate their efficacy.One is an integrated cell absorption quantitative polymerase chain reaction(qPCR)method(ICA-qPCR),and the other is a combined propidium monoazide qPCR method(PMA-qPCR).The quantification limit is 100 cell culture infective dose 50%(CCID50)/mL in ICA-qPCR following a 1.5-hour cell absorption or 126 CCID50/mL after a 15-minute incubation.For PMA-qPCR,the limit was 2,512 CCID50/mL.The number of genome copies quantified by the ICA-qPCR and PMA-qPCR methods was strongly correlated with the infectious titer determined by the CCID50 assay,thereby enabling the estimation of virus infectivity.The ICA-qPCR and PMA-qPCR methods are both suitable for the identification and quantification of intact infectious H1N1 virus in inactivated samples,wastewater,and biological materials.In conclusion,the ICA-qPCR and PMA-qPCR methods have distinct advantages and disadvantages,and can be used to quantify intact infectious viruses rapidly.These methodologies can facilitate the identification of the presence of intact infectious viruses in wastewater or on pathogen-related physical surfaces in high-level biosafety laboratories and medical facilities.Furthermore,these methodologies can also be utilized to detect other highly pathogenic pathogens.展开更多
Parvovirus B19(B19V)infection can cause pure red cell aplasia(PRCA)in patients with human immunodeficiency virus(HIV)infection.Intravenous immunoglobulin(IVIG)is a preferred treatment option.From July 2019 to March 20...Parvovirus B19(B19V)infection can cause pure red cell aplasia(PRCA)in patients with human immunodeficiency virus(HIV)infection.Intravenous immunoglobulin(IVIG)is a preferred treatment option.From July 2019 to March 2022,four patients with HIV infection were admitted to Guangzhou Eighth People’s Hospital with dizziness and fatigue and were diagnosed with PRCA.Blood investigations revealed severe anemia and the B19V genome.Therefore,the four patients were diagnosed with B19V-induced PRCA.All four patients received red blood cell transfusion in the setting of antiretroviral therapy,and two of the four patients received intravenous immunoglobulin(IVIG).After 3-7 months of treatment,all four patients recovered,although two did not receive IVIG.This suggests that IVIG is not always necessary for the treatment of PRCA in patients with HIV infection and that effective antiretroviral therapy and immunological reconstitution play an important role in the eradication of parvovirus.展开更多
基金supported by the National Key Research and Development Program of China (Nos. 2016YFE0203200, 2017YFD0500704)the Major Project of National Natural Science Foundation of China (No. 31430087)the Modern Agro-industry Technology Research System (No. CARS-41-G15)
文摘Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However.
基金supported by National key R&D projects(2022YFC2302701)Science and Technology Planning Project of Guangdong Province(2021B1212040017)Sun Yat-sen University Founded Program(2022_76220_B21127).
文摘The rarity of Francisella novicida infection in humans is well-known,and the F.novicida cases occur in immunocompromised patients or those with underlying health problems.Herein,we report the case of a patient with long-term diabetes who died following F.novicida infection that caused multiple organ failure,although F.novicida was effectively eliminated using antimicrobial therapy.Microbiological confirmation of F.novicida infection relies on metagenomic next-generation sequencing(mNGS)and pdpD-2 gene-specific identification.This study highlights the importance of early pathogen diagnosis in severely infected patients,particularly in cases of F.novicida,and indicates that mNGS is a useful tool for early diagnosis.
基金supported by Key-Area Research and Development Program of Guangdong Province,China(2022B1111010004,2021B1212040017)Basic Research Program of Guangzhou Municipal Science and Technology Bureau(SL2023A03J01074)+1 种基金Guangdong Provincial Highly Pathogenic Microorganism Science Data Center(2024B1212070013)Shenzhen Science and Technology Program(ZDSYS20230626091203007).
文摘The increase in emerging and reemerging infectious diseases has underscored the need for the prompt monitoring of intact infectious viruses and the quick assessment of their infectivity.However,molecular techniques cannot distinguish between intact infectious and noninfectious viruses.Here,two distinct methodologies have been developed for the expeditious and dependable quantification of intact infectious H1N1 virus,and several experiments have been conducted to substantiate their efficacy.One is an integrated cell absorption quantitative polymerase chain reaction(qPCR)method(ICA-qPCR),and the other is a combined propidium monoazide qPCR method(PMA-qPCR).The quantification limit is 100 cell culture infective dose 50%(CCID50)/mL in ICA-qPCR following a 1.5-hour cell absorption or 126 CCID50/mL after a 15-minute incubation.For PMA-qPCR,the limit was 2,512 CCID50/mL.The number of genome copies quantified by the ICA-qPCR and PMA-qPCR methods was strongly correlated with the infectious titer determined by the CCID50 assay,thereby enabling the estimation of virus infectivity.The ICA-qPCR and PMA-qPCR methods are both suitable for the identification and quantification of intact infectious H1N1 virus in inactivated samples,wastewater,and biological materials.In conclusion,the ICA-qPCR and PMA-qPCR methods have distinct advantages and disadvantages,and can be used to quantify intact infectious viruses rapidly.These methodologies can facilitate the identification of the presence of intact infectious viruses in wastewater or on pathogen-related physical surfaces in high-level biosafety laboratories and medical facilities.Furthermore,these methodologies can also be utilized to detect other highly pathogenic pathogens.
基金Guangzhou Basic Research Program on People’s Livelihood Science and Technology(202002020005)National Natural Science Foundation of China(82072265)+1 种基金Technology Planning Project of Guangdong Province(2021B1212040017)Sun Yat-sen University Founded Program(2022_76220_B21127)。
文摘Parvovirus B19(B19V)infection can cause pure red cell aplasia(PRCA)in patients with human immunodeficiency virus(HIV)infection.Intravenous immunoglobulin(IVIG)is a preferred treatment option.From July 2019 to March 2022,four patients with HIV infection were admitted to Guangzhou Eighth People’s Hospital with dizziness and fatigue and were diagnosed with PRCA.Blood investigations revealed severe anemia and the B19V genome.Therefore,the four patients were diagnosed with B19V-induced PRCA.All four patients received red blood cell transfusion in the setting of antiretroviral therapy,and two of the four patients received intravenous immunoglobulin(IVIG).After 3-7 months of treatment,all four patients recovered,although two did not receive IVIG.This suggests that IVIG is not always necessary for the treatment of PRCA in patients with HIV infection and that effective antiretroviral therapy and immunological reconstitution play an important role in the eradication of parvovirus.
