Objectives:Rural areas are the weakest place for epidemic prevention and control,yet few studies have specifically conducted surveys in rural areas.The purpose of this study is to assess rural residents’knowledge of ...Objectives:Rural areas are the weakest place for epidemic prevention and control,yet few studies have specifically conducted surveys in rural areas.The purpose of this study is to assess rural residents’knowledge of the COVID-19 and its prevention in China.Methods:A cross-sectional survey study containing 1,426 samples was conducted in Fuqing,China to assess rural residents’knowledge of the COVID-19 and its prevention.Logistic regression was used to identify potential differences in participants’knowledge of COVID-19 and its prevention and control in different population subgroups.Results:The mean and median of residents’knowledge of COVID-19 was 5.53 and 6 points,respectively.The mean and median of residents’knowledge related to self-protection against COVID-19 was 10.34 and 11 points,respectively.Older adults(AOR45e59=2.26,95%CI 1.20 to 4.27;AOR60-69=3.13,95%CI 1.63 to 5.98;AOR70=4.68,95%CI 2.35 to 9.33)were more deficient in knowledge of COVID-19.Those who were better educated and with a higher average annual household income were less likely to be deficient in the knowledge of COVID-19 and knowledge of self-protection against COVID-19.Moreover,those who were single,divorced,or widowed(AOR=1.67,95%CI 1.13 to 2.46)were more deficient in the knowledge of self-protection against COVID-19.Conclusions:Our study suggests that when facing a pandemic like COVID-19,the key is to inform the public to understand simple and effective self-protection measures.Therefore,we call on the govern-ments to give priority to publicity and education on self-prevention measures for the targeted pop-ulations and regions.This is most evident among the vulnerable groups like those who were unmarried,elderly,and those with low education or low income.Similar suggestions may be of use in other countries as well.展开更多
Quantitative real-time polymerase chain reaction(qRT-PCR)is a rapid and effective approach toward detecting the expression patterns of target genes.The selection of a stable reference gene under specific test conditio...Quantitative real-time polymerase chain reaction(qRT-PCR)is a rapid and effective approach toward detecting the expression patterns of target genes.The selection of a stable reference gene under specific test condition is essential for expressing levels of target genes accurately.Tilia miqueliana,considered endangered,is a prominent native ornamental and honey tree in East China.No study has evaluated the optimal endogenous reference gene for qRT-PCR analysis in T.miqueliana systematically.In this study,fifteen commonly used reference genes were selected as candidate genes,and the stabilities of their expressions were assessed using four algorithms(GeNorm,NormFiner,BestKeeper,and DeltaCt)in nine experimental datasets.The final integrated evaluation was performed using a comprehensive analysis algorithm(RefFinder).Finally,a target MYB transcription factor gene(TmMYB)was used to verify the accuracy of the candidate reference genes.The results showed that PP2αwas the most stable in tissue set,while RPS13 and SAMCD were optimal for heat and cold stress,respectively.Under waterlogging stress,PP2αand TUB were the most stable genes in the leaves and roots,respectively.EF1αand PP2αwere optimal for drying stress in leaf and root tissues.TUB and ACT7 were the most stable genes in the leaf and root tissues under salt stress.This is the first systematic evaluation of candidate reference genes in T.miqueliana,and it will benefit future studies on expression and functional analysis of target genes in T.miqueliana.展开更多
Objective This study aims to investigate the prevalence of familial cerebral cavernous malformations(FCCMs)in first-degree relatives(FDRs)using familial screening,to describe the distribution of initial symptoms,lesio...Objective This study aims to investigate the prevalence of familial cerebral cavernous malformations(FCCMs)in first-degree relatives(FDRs)using familial screening,to describe the distribution of initial symptoms,lesion count on cranial MRI and pathogenic gene in patients.Methods Patients with multiple CCMs who enrolled from the Treatments and Outcomes of Untreated Cerebral Cavernous Malformations in China database were considered as probands and FDRs were recruited.Cranial MRI was performed to screen the CCMs lesions,and whole-exome sequencing was performed to identify CCM mutations.MRI and genetic screening were combined to diagnose FCCM in FDRs,and the results were presented as prevalence and 95%CIs.The Kaplan-Meier(KM)method was used to calculate the cumulative incidence of FCCM.Results 33(76.74%)of the 43 families(110 FDRs)were identified as FCCM(85 FDRs).Receiver operating characteristic analysis revealed three lesions on T2-weighted imaging(T2WI)were the strong indicator for distinguishing probands with FCCM(sensitivity,87.10%;specificity,87.50%).Of the 85 FDRs,31 were diagnosed with FCCM,resulting in a prevalence of 36.5%(26.2%-46.7%).In families with FCCMs,the mutation rates for CCM1,CCM2 and CCM3 were 45.45%,21.21%and 9.09%,respectively.Furthermore,53.13%of patients were asymptomatic,17.19%were intracranial haemorrhage and 9.38%were epilepsy.The mean age of symptom onset analysed by KM was 46.67(40.56-52.78)years.Conclusion Based on MRI and genetic analysis,the prevalence of CCMs in the FDRs of families with FCCMs in China was 36.5%.Genetic counselling and MRI screening are recommended for FDRs in patients with more than three CCM lesions on T2WI.展开更多
Evidence for the influence of chronic inflammation induced by microbial dysbiosis on aberrant DNA methylation supports a plausible connexion between disordered microbiota and precancerous lesions of gastric cancer(PLG...Evidence for the influence of chronic inflammation induced by microbial dysbiosis on aberrant DNA methylation supports a plausible connexion between disordered microbiota and precancerous lesions of gastric cancer(PLGC).Here,a comprehensive study including multi-omics data was performed to estimate the relationships amongst the gastric microbiome,inflammatory proteins and DNA methylation alterations and their roles in PLGC development.The results demonstrated that gastric dysbacteriosis increased the risk of PLGC and DNA methylation alterations in related tumour suppressor genes.Seven inflammatory biomarkers were identified for antrum and corpus tissues,respectively,amongst which the expression levels of several biomarkers were significantly correlated with the microbial dysbiosis index(MDI)and methylation status of specific tumour suppressor genes.Notably,mediation analysis revealed that microbial dysbiosis partially contributed to DNA methylation changes in the stomach via the inflammatory cytokines C-C motif chemokine 20(CCL20)and tumour necrosis factor receptor superfamily member 9(TNFRSF9).Overall,these results may provide new insights into the mechanisms that might link the gastric microbiome to PLGC.展开更多
基金supported by funding from Fujian Medical University(Grant No.XRCZX 2020020Grant No.XRZCX 2017035,Grant No.XRZCX 2020034).
文摘Objectives:Rural areas are the weakest place for epidemic prevention and control,yet few studies have specifically conducted surveys in rural areas.The purpose of this study is to assess rural residents’knowledge of the COVID-19 and its prevention in China.Methods:A cross-sectional survey study containing 1,426 samples was conducted in Fuqing,China to assess rural residents’knowledge of the COVID-19 and its prevention.Logistic regression was used to identify potential differences in participants’knowledge of COVID-19 and its prevention and control in different population subgroups.Results:The mean and median of residents’knowledge of COVID-19 was 5.53 and 6 points,respectively.The mean and median of residents’knowledge related to self-protection against COVID-19 was 10.34 and 11 points,respectively.Older adults(AOR45e59=2.26,95%CI 1.20 to 4.27;AOR60-69=3.13,95%CI 1.63 to 5.98;AOR70=4.68,95%CI 2.35 to 9.33)were more deficient in knowledge of COVID-19.Those who were better educated and with a higher average annual household income were less likely to be deficient in the knowledge of COVID-19 and knowledge of self-protection against COVID-19.Moreover,those who were single,divorced,or widowed(AOR=1.67,95%CI 1.13 to 2.46)were more deficient in the knowledge of self-protection against COVID-19.Conclusions:Our study suggests that when facing a pandemic like COVID-19,the key is to inform the public to understand simple and effective self-protection measures.Therefore,we call on the govern-ments to give priority to publicity and education on self-prevention measures for the targeted pop-ulations and regions.This is most evident among the vulnerable groups like those who were unmarried,elderly,and those with low education or low income.Similar suggestions may be of use in other countries as well.
基金the Natural Science Foundation of Jiangsu Province under Grant No.BK20170619National Natural Science Foundation of China under Grant No.31700477Innovation and Extension of Forestry Science and Technology of Jiangsu Province under Grant No.LYKJ[2019]06.
文摘Quantitative real-time polymerase chain reaction(qRT-PCR)is a rapid and effective approach toward detecting the expression patterns of target genes.The selection of a stable reference gene under specific test condition is essential for expressing levels of target genes accurately.Tilia miqueliana,considered endangered,is a prominent native ornamental and honey tree in East China.No study has evaluated the optimal endogenous reference gene for qRT-PCR analysis in T.miqueliana systematically.In this study,fifteen commonly used reference genes were selected as candidate genes,and the stabilities of their expressions were assessed using four algorithms(GeNorm,NormFiner,BestKeeper,and DeltaCt)in nine experimental datasets.The final integrated evaluation was performed using a comprehensive analysis algorithm(RefFinder).Finally,a target MYB transcription factor gene(TmMYB)was used to verify the accuracy of the candidate reference genes.The results showed that PP2αwas the most stable in tissue set,while RPS13 and SAMCD were optimal for heat and cold stress,respectively.Under waterlogging stress,PP2αand TUB were the most stable genes in the leaves and roots,respectively.EF1αand PP2αwere optimal for drying stress in leaf and root tissues.TUB and ACT7 were the most stable genes in the leaf and root tissues under salt stress.This is the first systematic evaluation of candidate reference genes in T.miqueliana,and it will benefit future studies on expression and functional analysis of target genes in T.miqueliana.
基金supported by grants from Technology Platform Construction Project of Fujian Province(2020Y2003,2021Y2001)supported the Fujian Province High level Neuromedical Center Construction Fund(principal investigator:DK),a grant from the Government of Fujian Province(grant number:HLNCC-FJFY 003)supported by grants from National Natural Science Foundation of China(8227051360).
文摘Objective This study aims to investigate the prevalence of familial cerebral cavernous malformations(FCCMs)in first-degree relatives(FDRs)using familial screening,to describe the distribution of initial symptoms,lesion count on cranial MRI and pathogenic gene in patients.Methods Patients with multiple CCMs who enrolled from the Treatments and Outcomes of Untreated Cerebral Cavernous Malformations in China database were considered as probands and FDRs were recruited.Cranial MRI was performed to screen the CCMs lesions,and whole-exome sequencing was performed to identify CCM mutations.MRI and genetic screening were combined to diagnose FCCM in FDRs,and the results were presented as prevalence and 95%CIs.The Kaplan-Meier(KM)method was used to calculate the cumulative incidence of FCCM.Results 33(76.74%)of the 43 families(110 FDRs)were identified as FCCM(85 FDRs).Receiver operating characteristic analysis revealed three lesions on T2-weighted imaging(T2WI)were the strong indicator for distinguishing probands with FCCM(sensitivity,87.10%;specificity,87.50%).Of the 85 FDRs,31 were diagnosed with FCCM,resulting in a prevalence of 36.5%(26.2%-46.7%).In families with FCCMs,the mutation rates for CCM1,CCM2 and CCM3 were 45.45%,21.21%and 9.09%,respectively.Furthermore,53.13%of patients were asymptomatic,17.19%were intracranial haemorrhage and 9.38%were epilepsy.The mean age of symptom onset analysed by KM was 46.67(40.56-52.78)years.Conclusion Based on MRI and genetic analysis,the prevalence of CCMs in the FDRs of families with FCCMs in China was 36.5%.Genetic counselling and MRI screening are recommended for FDRs in patients with more than three CCM lesions on T2WI.
基金funded by Grant of Science and Technology of Fujian,China(2019L3006)Special Funds of Fujian Finance Department(2020czbz01)High-level Talents Research Start-up Project of Fujian Medical University(XRCZX2017035 and XRCZX2020034).
文摘Evidence for the influence of chronic inflammation induced by microbial dysbiosis on aberrant DNA methylation supports a plausible connexion between disordered microbiota and precancerous lesions of gastric cancer(PLGC).Here,a comprehensive study including multi-omics data was performed to estimate the relationships amongst the gastric microbiome,inflammatory proteins and DNA methylation alterations and their roles in PLGC development.The results demonstrated that gastric dysbacteriosis increased the risk of PLGC and DNA methylation alterations in related tumour suppressor genes.Seven inflammatory biomarkers were identified for antrum and corpus tissues,respectively,amongst which the expression levels of several biomarkers were significantly correlated with the microbial dysbiosis index(MDI)and methylation status of specific tumour suppressor genes.Notably,mediation analysis revealed that microbial dysbiosis partially contributed to DNA methylation changes in the stomach via the inflammatory cytokines C-C motif chemokine 20(CCL20)and tumour necrosis factor receptor superfamily member 9(TNFRSF9).Overall,these results may provide new insights into the mechanisms that might link the gastric microbiome to PLGC.
