Biological robot is a kind of creature controlled by human beings by applying intervention signals through control technology to regulate biological behavior.At present,the research on bio-robot mainly focuses on terr...Biological robot is a kind of creature controlled by human beings by applying intervention signals through control technology to regulate biological behavior.At present,the research on bio-robot mainly focuses on terrestrial mammals and insects,while the research on aquatic animal robot is less.Early studies have shown that the medial longitudinal fasciculus nucleus(NFLM)of carp midbrain was related to tail wagging,but the research has not been applied to the navigation control of the carp robot.The purpose of this study is to realize the quantitative control of the forward and steering behavior of the carp robot by NFLM electrical stimulation.Under the condition of no craniotomy,brain electrode was implanted into the NFLM of the carp midbrain,and the underwater control experiment was carried out by applying different electrical stimulation parameters.Using the ImageJ software and self-programmed,the forward motion speed and steering angle of steering motion of the carp robot before and after being stimulated were calculated.The experimental results showed for the carp robot that was induced the steering motion,the left and right steering motion of 30°to 150°could be achieved by adjusting the stimulation parameters,for the carp robot that was induced the forward motion,the speed of forward motion could be controlled to reach 100 cm/s.The research lays a foundation for the accurate control of the forward and steering motion of the aquatic animal robot.展开更多
Brain-controlled technology is the key technology in biological control,and the corresponding relationship between animal brain nuclei and motor behavior is the core.The purpose of this study was to explore the motor ...Brain-controlled technology is the key technology in biological control,and the corresponding relationship between animal brain nuclei and motor behavior is the core.The purpose of this study was to explore the motor nuclei of the medulla oblongata in carp.The carps were subjected to electrical stimulation and chemical stimulation experiments,respectively,in the water-free state,and the effective chemical stimulation sites were injected with the pontamine sky blue solution.The brain tissue sections were obtained by paraffin tissue section technology and the neutral red staining method.By comparing the positions of the brain nuclei shown in earlier studies,the motor nerve nuclei in the medulla oblongata were identified.The brain electrode was implanted into the motor nucleus of the medulla oblongata,and the underwater control experiment and behavioral tests were carried out with different electrical stimulation parameters.The results showed that the abducens nucleus(NVI)was the motor nucleus that controls the ipsilateral steering,and the facial nucleus(NVII)was the motor nucleus that controls the forward movement.By adjusting the stimulation voltage and the stimulation pulse number,the carp can be stably controlled to achieve a left–right steering motion of 30°–180°and a forward motion of more than 80 cm/s.This study indicated that the quantitative control of the steering and forward behavior of the carp can be achieved by electrical stimulation of the NVI and NVII,which provided a certain experimental basis for the accurate control of the carp robot.展开更多
基金This work was financially supported by the Project of National Natural Science Foundation of China(project number:61573305)Projectof Natural Science Foundation of Hebei Provinceof China(project number:F2019203511)National High-Tech Research and Development Plan of China(863 Plan)Project(2013AA***)Fund.
文摘Biological robot is a kind of creature controlled by human beings by applying intervention signals through control technology to regulate biological behavior.At present,the research on bio-robot mainly focuses on terrestrial mammals and insects,while the research on aquatic animal robot is less.Early studies have shown that the medial longitudinal fasciculus nucleus(NFLM)of carp midbrain was related to tail wagging,but the research has not been applied to the navigation control of the carp robot.The purpose of this study is to realize the quantitative control of the forward and steering behavior of the carp robot by NFLM electrical stimulation.Under the condition of no craniotomy,brain electrode was implanted into the NFLM of the carp midbrain,and the underwater control experiment was carried out by applying different electrical stimulation parameters.Using the ImageJ software and self-programmed,the forward motion speed and steering angle of steering motion of the carp robot before and after being stimulated were calculated.The experimental results showed for the carp robot that was induced the steering motion,the left and right steering motion of 30°to 150°could be achieved by adjusting the stimulation parameters,for the carp robot that was induced the forward motion,the speed of forward motion could be controlled to reach 100 cm/s.The research lays a foundation for the accurate control of the forward and steering motion of the aquatic animal robot.
基金supported by the Project of National Natural Science Foundation of China(project number:61573305)Project of Natural Science Foundation of Hebei Province of China(project number:F2022203038,F2019203511)National High-Tech Research and Development Plan of China(863 Plan)Project(2013AA****)Fund.
文摘Brain-controlled technology is the key technology in biological control,and the corresponding relationship between animal brain nuclei and motor behavior is the core.The purpose of this study was to explore the motor nuclei of the medulla oblongata in carp.The carps were subjected to electrical stimulation and chemical stimulation experiments,respectively,in the water-free state,and the effective chemical stimulation sites were injected with the pontamine sky blue solution.The brain tissue sections were obtained by paraffin tissue section technology and the neutral red staining method.By comparing the positions of the brain nuclei shown in earlier studies,the motor nerve nuclei in the medulla oblongata were identified.The brain electrode was implanted into the motor nucleus of the medulla oblongata,and the underwater control experiment and behavioral tests were carried out with different electrical stimulation parameters.The results showed that the abducens nucleus(NVI)was the motor nucleus that controls the ipsilateral steering,and the facial nucleus(NVII)was the motor nucleus that controls the forward movement.By adjusting the stimulation voltage and the stimulation pulse number,the carp can be stably controlled to achieve a left–right steering motion of 30°–180°and a forward motion of more than 80 cm/s.This study indicated that the quantitative control of the steering and forward behavior of the carp can be achieved by electrical stimulation of the NVI and NVII,which provided a certain experimental basis for the accurate control of the carp robot.
