With the intelligent upgrading of manufacturing equipment,achieving high-precision and efficient fault diagnosis is essential to enhance equipment stability and increase productivity.Online monitoring and fault diagno...With the intelligent upgrading of manufacturing equipment,achieving high-precision and efficient fault diagnosis is essential to enhance equipment stability and increase productivity.Online monitoring and fault diagnosis technology play a critical role in improving the stability of metal additive manufacturing equipment.However,the limited proportion of fault data during operation challenges the accuracy and efficiency of multi-classification models due to excessive redundant data.A multi-sensor and principal component analysis(PCA)and support vector machine(SVM)asymptotic classification(PCSV)for additive manufacturing fault diagnosis method is proposed,and it divides the fault diagnosis into two steps.In the first step,real-time data are evaluated using the T2 and Q statistical parameters of the PCAmodel to identify potential faults while filtering non-fault data,thereby reducing redundancy and enhancing real-time efficiency.In the second step,the identified fault data are input into the SVM model for precise multi-class classification of fault categories.The PCSV method advances the field by significantly improving diagnostic accuracy and efficiency,achieving an accuracy of 99%,a diagnosis time of 0.65 s,and a training time of 503 s.The experimental results demonstrate the sophistication of the PCSV method for high-precision and high-efficiency fault diagnosis of small fault samples.展开更多
Fatigue properties of materials by Additive Manufacturing(AM) depend on many factors such as AM processing parameter, microstructure, residual stress, surface roughness, porosities, post-treatments, etc. Their evaluat...Fatigue properties of materials by Additive Manufacturing(AM) depend on many factors such as AM processing parameter, microstructure, residual stress, surface roughness, porosities, post-treatments, etc. Their evaluation inevitably requires these factors combined as many as possible, thus resulting in low efficiency and high cost. In recent years, their assessment by leveraging the power of Machine Learning(ML) has gained increasing attentions. A comprehensive overview on the state-of-the-art progress of applying ML strategies to predict fatigue properties of AM materials, as well as their dependence on AM processing and post-processing parameters such as laser power, scanning speed, layer height, hatch distance, built direction, post-heat temperature,etc., were presented. A few attempts in employing Feedforward Neural Network(FNN), Convolutional Neural Network(CNN), Adaptive Network-Based Fuzzy Inference System(ANFIS), Support Vector Machine(SVM) and Random Forest(RF) to predict fatigue life and RF to predict fatigue crack growth rate are summarized. The ML models for predicting AM materials' fatigue properties are found intrinsically similar to the commonly used ones, but are modified to involve AM features. Finally, an outlook for challenges(i.e., small dataset, multifarious features,overfitting, low interpretability, and unable extension from AM material data to structure life) and potential solutions for the ML prediction of AM materials' fatigue properties is provided.展开更多
The remarkable capabilities of 2D plasmonic surfaces in controlling optical waves havegarnered significant attention.However,the challenge of large-scale manufacturing of uniform,well-aligned,and tunable plasmonic sur...The remarkable capabilities of 2D plasmonic surfaces in controlling optical waves havegarnered significant attention.However,the challenge of large-scale manufacturing of uniform,well-aligned,and tunable plasmonic surfaces has hindered their industrialization.To address this,we present a groundbreaking tunable plasmonic platform design achieved throughmagnetic field(MF)assisted ultrafast laser direct deposition in air.Through precise control of metal nanoparticles(NPs),with cobalt(Co)serving as the model material,employing an MF,and fine-tuning ultrafast laser parameters,we have effectively converted coarse and non-uniform NPs into densely packed,uniform,and ultrafine NPs(~3 nm).This revolutionary advancement results in the creation of customizable plasmonic‘hot spots,’which play a pivotal role insurface-enhanced Raman spectroscopy(SERS)sensors.The profound impact of this designable plasmonic platform lies in its close association with plasmonic resonance and energyenhancement.When the plasmonic nanostructures resonate with incident light,they generate intense local electromagnetic fields,thus vastly increasing the Raman scattering signal.This enhancement leads to an outstanding 2–18 fold boost in SERS performance and unparalleled sensing sensitivity down to 10^(-10)M.Notably,the plasmonic platform also demonstratesrobustness,retaining its sensing capability even after undergoing 50 cycles of rinsing andre-loading of chemicals.Moreover,this work adheres to green manufacturing standards,making it an efficient and environmentally friendly method for customizing plasmonic‘hot spots’inSERS devices.Our study not only achieves the formation of high-density,uniform,and ultrafine NP arrays on a tunable plasmonic platform but also showcases the profound relation betweenplasmonic resonance and energy enhancement.The outstanding results observed in SERS sensors further emphasize the immense potential of this technology for energy-relatedapplications,including photocatalysis,photovoltaics,and clean water,propelling us closer to a sustainable and cleaner future.展开更多
Bacterial biofilms(BFs)are widely present in nature,and more than 99%of bacteria can form BF,which is an important factor leading to persistent infection of refractory wound and repeated infection.The formation of BF i...Bacterial biofilms(BFs)are widely present in nature,and more than 99%of bacteria can form BF,which is an important factor leading to persistent infection of refractory wound and repeated infection.The formation of BF is a dynamic cyclic process involving various physical,chemical and biological processes,mainly including bacterial attachment,BF formation and maturation,and bacterial diffusion.The bacteria in bacterial biofilms are more resistant to antibiotics and disinfectants,and more resilient to environmental changes,which presents many challenges in treatment.This article reviews the basic characteristics,resistance mechanisms and treatment strategies of BF.At present,there are many studies on the treatment of BF,which need to be selected according to the specific situation and pathophysiological process of wound infection,and can be used as a single method or in combination.This article introduces some treatment methods to provide a reference for the clinical prevention and treatment of BF.展开更多
The nonlinear cumulative damage model is modified to have high prediction accuracy when the high-low cycle stress frequency ratio m is large(m500).The low cycle fatigue(LCF)tests,high cycle fatigue(HCF)tests and combi...The nonlinear cumulative damage model is modified to have high prediction accuracy when the high-low cycle stress frequency ratio m is large(m500).The low cycle fatigue(LCF)tests,high cycle fatigue(HCF)tests and combined high and low cycle fatigue(CCF)tests of TC11 titanium alloy were carried out,and the influencing factors of CCF life were analysed.The CCF life declines with the decrease of the ratio of high-low cycle stress frequency m.Both linear and nonlinear cumulative damage models are used to predict the CCF life.The CCF life prediction error of the linear cumulative damage model is great and the predictions tend to be overestimated,which is dangerous for engineering application.The accuracy is relatively high when the high-low cycle stress frequency ratio m500.The accuracy of nonlinear cumulative damage model is higher than that of linear model when the high-low cycle stress frequency ratio m500.Based on the relationship between high cycle average stress rmajor and material yield limit rp,0.2,a correction term is added to the nonlinear cumulative damage model and verified,which made the modified model more accurate when m500.展开更多
Burn as a strong traumatic stimulus can induce inflammatory responses mediated by neuroendocrine,cytokines and other inflammatory mediators,and directly lead to organ dysfunction,resulting in severe MODS.Excessive inf...Burn as a strong traumatic stimulus can induce inflammatory responses mediated by neuroendocrine,cytokines and other inflammatory mediators,and directly lead to organ dysfunction,resulting in severe MODS.Excessive inflammatory responses caused by necrotic tissues in the burned wound,also play an important role in stimulating the occurrence and the development of MODS.Complete removal of necrotic tissues in the early stage of burns to prevent from invasive wound infections,can effectively prevent MODS.AKI occurred earlier in this case,combined with its admission after the onset of shock and no obstruction of the urinary system can exclude prerenal and renal AKI,speculated as renal AKI.The factors leading to renal AKI are divided into renal ischemia and nephrotoxicity.The patient had adequate urinary output during shock and AKI in the presence of adequate renal blood flow,possibly due to the nephrotoxicity of a large number of necrotic tissues of the wound after they were broken down and absorbed into the bloodstream.The patient fell asleep after being drunk and the bedside stove broiled him for a long time,on one hand,heat accumulation lead to skin damage;on the other hand,sustained heat action caused hyperemia,edema and hemodynamic disorder in the deep tissues,and then resulted in secondary necrosis.展开更多
Since the discovery of adipose-derived mesenchymal stem cell (ADSC) in more than ten years, a great progress has been made from its basic research to clinical application. Compared with bone marrow mesenchymal stem ce...Since the discovery of adipose-derived mesenchymal stem cell (ADSC) in more than ten years, a great progress has been made from its basic research to clinical application. Compared with bone marrow mesenchymal stem cells, ADSC is more abundant in reserve, easier to obtain with fewer injuries and less complications. These cells have multiple differentiation potential and can differentiate into adipocytes, chondrocytes and osteoblasts with the influence of different inducing factors. Early studies of ADSC mainly focused on the ability of multi-directional differentiation, especially on the regeneration of bone defects and cartilage tissue. At present, the researches mainly focus on immunoregulation and paracrine function of ADSC. Although ADSC has made a great progress in clinical application, the cell preparation, use pattern, and mechanisms in clinical treatment are not clear. This paper elaborates on these issues.展开更多
China began using the colored liquid from tea and tincture to treat burns as early as the sixth century B.C.,and records of Chinese medicine for the rescue and treatment of burns date from the Jin Dynasty to the Ming ...China began using the colored liquid from tea and tincture to treat burns as early as the sixth century B.C.,and records of Chinese medicine for the rescue and treatment of burns date from the Jin Dynasty to the Ming and Qing Dynasties.Modern burn treatment medicine in China began in 1958 when national steel production began.Since then,burn patients have increased dramatically,independent burn departments have been established nationwide,and burn surgery has moved into technological development.With the combined efforts of several generations of burn medical workers in China,the clinical treatment of severe and extra-severe burns has steadily increased.Many fields,such as burn shock prevention and treatment,wound repair,and sepsis,have become world leaders in the 21st century,forming a treatment technology and theoretical system with distinctive Chinese characteristics.Burn medical professionals have conducted numerous clinical and fundamental experimental studies,founded the Burns Surgery Branch of the Chinese Medical Association,established academic journals,and successfully established China Burn Treatment Program.The advancement of science and technology has promoted the development of modern medicine,and 3D virtual reality technology,3D printing technology,organoid culture,and tissue engineering skin have demonstrated potential advantages and excellent application prospects in the treatment of severe burns,providing new ideas and directions for the discipline’s development.In the new journey of development and reform,we should continue to strengthen the systematic construction of the discipline,enhance the innovative treatment capability,and create further triumph.展开更多
Objective: To explore the effects of combined application of culture supernatant of human umbilical cord mesenchymal stem cells (hUCMSCs) and ciprofloxacin on Staphylococcus aureus (SA) in vitro. Methods: hUCMSCs were...Objective: To explore the effects of combined application of culture supernatant of human umbilical cord mesenchymal stem cells (hUCMSCs) and ciprofloxacin on Staphylococcus aureus (SA) in vitro. Methods: hUCMSCs were isolated from umbilical cord tissues of full-term healthy fetuses after cesarean section and then cultured. Cells in the third passage were chosen for the use of experiment after identification. SA strains isolated from wounds of burn patients in our burn wards were used in the following experiment. Cells were divided into 0, 10, 100 and 1,000 ng/ml lipopolysaccharide (LPS) groups by use of the random number table (similarly hereinafter). Cells were cultured with culture medium containing mesenchymal stem cells (MSCs) after being treated with medium containing corresponding mass concen-trations of LPS for 12 h. At post culture hour (PCH) 6, 12 and 24, 6 wells of culture supernatant of cells in each group were obtained to measure the content of LL-37 with enzyme-linked immunosorbent assay (ELISA). Ninety blood agar culture plates were divided into ciprofloxacin control group (CC), ciprofloxacin + supernatant group (CS), and ciprofloxacin + supernatant+ LL-37 antibody group (CSL), with 30 blood agar culture plates in each group. Blood agar culture plates in group CC were coated with 1.5 × 108 colony forming unit (CFU)/ml bacteria solution prepared with normal saline. Blood agar culture plates in group CS were coated with 1.5 × 108 CFU/ml bacteria solution prepared with normal saline and hUCMSC culture supernatant (cultured by MSC culture medium, the same below) in double volume of normal saline. Blood agar culture plates in group CSL were coated with 1.5 × 108 CFU/ml bacteria solution prepared with normal saline, hUCMSC culture supernatant in double volume of normal saline, and 2.6 μL of LL-37 antibodies at the concentration of 2 μg/ml. At PCH 12, 24 and 48, 10 blood agar culture plates were taken out from each group to observe the distribution of SA colonies on blood agar culture plates and to measure diameters of zones of inhibition of ciprofloxacin. The minimum inhibitory concentration (MIC) of ciprofloxacin against SA in each group was recorded. Fractional inhibitory concentration (FIC) indexes of ciprofloxacin in group CS and group CSL at PCH 12, 24 and 48 were calculated, with the synergistic effect evaluated. Data were processed with factorial design ANOVA, one way ANOVA, LSD-t test, Kruskal-Wallis test and Mann-Whitney U test. Results: (1) At each PCH, the content of LL-37 in cell culture supernatant in 10 ng/ml LPS group, 100 ng/ml LPS group or 1,000 ng/ml LPS group was higher than that in 0 ng/ml LPS group (with t values ranging from 11.22 to 33.36, p values all below .01);the content of LL-37 in cell culture supernatant in either 100 ng/ml LPS group or 1,000 ng/ml LPS group was higher than that in 10 ng/ml LPS group (with t values ranging from 2.24 to 18.73, p < .05 or p < .01);the content of LL-37 in cell culture supernatant in 1,000 ng/ml LPS group was higher than that in 100 ng/ml LPS group (with t values ranging from 12.46 to 14.70, p values all below .01). (2) At PCH 12, 24 and 48, the bacterial colonies in groups CC, CS and CSL became integrated over time. In CC group, diameters of zones of inhibition of ciprofloxacin at PCH 12, 24 and 48 were 26 mm, 24 mm and 23 mm respectively, with no obvious changes. At PCH 12, 24 and 48, diameters of zones of inhibition of ciprofloxacin in groups CS and CSL were 82 mm, 71 mm, 68 mm and 74 mm, 59 mm, 56 mm respectively, which were significantly larger than those in group CC. (3) At each PCH, MIC of ciprofloxacin against SA in group CC was significantly higher than that in groups CS and CSL respectively (with Z values ranging from 6.22 to 6.71, p values all below .01);MIC of ciprofloxacin against SA in group CSL was significantly higher than that in group CS (with Z values all equal to 6.72, p values all below .01). (4) FIC indexes of ciprofloxacin in groups CS and CSL at PCH 12, 24 and 48 were 0.011, 0.032, 0.032 and 0.122, 0.350, 0.350, respectively. The results indicated that hUCMSC culture supernatant had a synergistically antibacterial effect when combined with ciprofloxacin. Conclusions: hUCMSCs can secrete LL-37, and the secretion level is improved with increase of LPS concentration. The com-bination of hUCMSC culture supernatant with ciprofloxacin can decrease the dosage of ciprofloxacin in resisting SA effectively. Once LL-37 is neutralized, the synergistically antibacterial effect of hUCMSC culture supernatant is decreased thereby.展开更多
Objective:To observe the protective effects and mechanisms of topical Chinese medicine on kidneys of severe burns shock rats.Methods:Blood urea nitrogen(Bun),creatinine(Cr),myeloperoxidase(MPO),tumor necrosis factor-...Objective:To observe the protective effects and mechanisms of topical Chinese medicine on kidneys of severe burns shock rats.Methods:Blood urea nitrogen(Bun),creatinine(Cr),myeloperoxidase(MPO),tumor necrosis factor-α(TNF-α)and inter-leukin(IL-6),were checked by the time points of 8,24,48 hours.Blood tests,ELISA and other methods were used for these texts.Results:Compared with the povidone-iodine group,the levels of Bun and Cr were declined apparently in the Sanhuang group,and the activity of MPO was decreased as well.Conclusions:Sanhuangshaoshangling,taking ointment as matrix,can be externally applied to wounds.It contains some ef-fective ingredients which can clear heat-toxicity and promote blood circulation for removing blood stasis,and prevents wounds from the stimulation of the air to nerve endings and the invasion of bacteria.It can also inhibit bacteria from parasitizing,grow-ing,reproducing on the wound.Sanhuangshaoshangling helps blood circulation and facilitates the growth of tissues to inactivate the toxicity or reduce toxin by changing the structure of endotoxin after uptake into tissues.It can protect viscera by reducing the release of endotoxin,facilitating the excretion of endotoxin and weakening or eliminating the effects of TNF-αand IL-6.展开更多
Cell therapy represents an important area of biotechnology,with the potential to treat and even cure a variety of diseases that cannot be cured by traditional therapies.Cell therapy is currently a widely studied treat...Cell therapy represents an important area of biotechnology,with the potential to treat and even cure a variety of diseases that cannot be cured by traditional therapies.Cell therapy is currently a widely studied treatment in both preclinical and clinical settings,and many cell therapy products are undergoing clinical trials.This article reviews the application of cell therapy in a variety of diseases and the policy of stem cell therapy in China.展开更多
Objective: To explore the significance of D-dimer assay in the diagnosis of acute pulmonary embolism (APE) after deep second-degree burn by detecting the changes of plasma D-dimer in rabbits with deep second-degree bu...Objective: To explore the significance of D-dimer assay in the diagnosis of acute pulmonary embolism (APE) after deep second-degree burn by detecting the changes of plasma D-dimer in rabbits with deep second-degree burn and in different phases of burn with concomitant pulmonary embolism. Methods: 48 healthy male or female Chinese white rabbits, were randomly divided into: control group, burn group, burn shock group, burn shock + pulmonary embolism group, burn infection group and burn infection + pulmonary embolism group, with 8 rabbits in each group. By use of the ELISA method, the plasma D-dimer was determined on 1 d, 3 d, 5 d, 7 d, 14 d and 21 d after modeling in the burn group and the control group, and the plasma D-dimer in other groups was detected in 30 min, 1 h, 3 h, 6 h, 24 h after modeling. Results: The expression of D-dimer in the burn group was higher than that in the control group on 1 d, 3 d, 5 d and 7 d (p < .05). There were significant differences in the expression of D-dimer in the burn shock + pulmonary embolism group in comparison to the burn shock group (p < .05). There were also some significant differences in the expression of D-dimer in the burn infection+ pulmonary embolism group in comparison with the burn shock + pulmonary embolism group (p < .05). Conclusions: There were some differences in the expression of D-dimer in each group. D-dimer can be used as one of diagnostic indexes for deep second-degree burn with pulmonary embolism.展开更多
Objective:To explore the potential protective effects of ulinastatin on ventilation-induced lung injuries of severe burned rats.Methods:Ninety Wistar rats were randomly divided into three experimental groups:the contr...Objective:To explore the potential protective effects of ulinastatin on ventilation-induced lung injuries of severe burned rats.Methods:Ninety Wistar rats were randomly divided into three experimental groups:the control group(n=30),the ventilation group(n=30)and the ventilation-ulinastatin group(n=30).After establishing the severe burn model,the rats of latter two groups were mechanically ventilated for 1 hour with or without the pre-treatment of ulinastatin.After severe scald,the protective effect of ulinastatin on lung injury caused by mechanical ventilation was estimated through the observation of the tissues samples,and evaluation of the pathological changes of lung tissue by HE staining,ultrastructure change by electron microscopy,lung coefficient,and the expression levels of lung tissue cytokines TNF-α,IFN-γ,IL-2 by immunohistochemical staining.Results:Edema in lung tissues of the control group and the ventilation group was obvious,the hemorrhagic focus could be seen,and the cut surface was observed to be scattered and swelling;Edema in lung tissues of the ventilation-ulinastatin group was mild.HE staining revealed that the pathological changes of the ventilation-ulinastatin group were milder than the ventilation group.Under the electron microscope,the lung tissue organelles of the control group and the ventilation group were seriously damaged;the corresponding changes in the ventilation-ulinastatin group were lighter.The lung coefficient of the ventilation-ulinastatin group was significantly lower than that in the ventilation group.The immunohistochemical results showed that the intensity of TNF-α,IL-2 and IFN-γin lung tissue of the ventilation-ulinastatin group was significantly lower than that in the ventilation group.Conclusions:Ulinastatin has protective effects on lung injury caused by mechanical ventilation in severe scalded rats,whose mechanism may be related to the capacity of ulinastatin to reduce the expression of cytokines including TNF-α,IL-2 and IFN-γ.展开更多
Objective:To investigate the expression of cycloxygenase-2(COX-2),and vascular endothelial growth factor(VEGF)by using immunohistochemistry method and observe the changes of histological structure between the all skin...Objective:To investigate the expression of cycloxygenase-2(COX-2),and vascular endothelial growth factor(VEGF)by using immunohistochemistry method and observe the changes of histological structure between the all skin group and the xenoskin(sheepskin)group,and explore the effect of the xenoskin(sheepskin)which is a kind of covering of autogenous microskin grafting.Methods:A total of 180 Wistar healthy male rats were randomly divided into 3 groups:the autoskin group(the control group),the alloskin group and the xenoskin group(the sheepskin group),with 60 rats in each group.By using immunohistochemistry method,the expression of COX-2 and VEGF were revealed on 3,5,7,14,21 and 28 d,with 10 executed rats in each group on each time points,and the structure of histology was observed by light microscope.Results:There were obvious difference of expression of COX-2 and VEGF on wound in the autoskin group compared with other two groups(p<.01);but there were no differences between the alloskin group and xenoskin group(p>.05).Observed on light microscope,the paralleled collagen and elastic fiber appeared in the autoskin group on 14 post-transplantation days,but they appeared in the alloskin group and the xenoskin group only on 21 post-transplantation days.Conclusions:There were no differences of the expression of COX-2 and VEGF in the alloskin group and the xenoskin group,which provided clinical theoretical basis on sheepskin as a substitute for the alloskin to cover autogenous microskin.展开更多
Inflammation is generally considered as a pathological process,and the occurrence of multi-organ inflammatory diseases is usually accompanied by an inflammatory response.Persistent inflammation can eventually lead to ...Inflammation is generally considered as a pathological process,and the occurrence of multi-organ inflammatory diseases is usually accompanied by an inflammatory response.Persistent inflammation can eventually lead to organ dysfunction.In recent years,the study of mesenchymal stem cell-derived exosomes has found its unique anti-inflammatory effect,which provides a new idea for the treatment of multi-organ inflammatory diseases.This article reviews the effect of mesenchymal stem cell exosomes on multi-organ inflammatory diseases.展开更多
Objective:To explore the burn treatment and first aid flow mode in batch patients.Methods:A retrospective analysis was performed on the treatment experience of 10 or more patients in the group burn accident admitted t...Objective:To explore the burn treatment and first aid flow mode in batch patients.Methods:A retrospective analysis was performed on the treatment experience of 10 or more patients in the group burn accident admitted to the Third Affiliated Hospital of Inner Mongolia Medical University from 2008 to 2020,in order to acquire the general information(e.g.,age,sex[male and female],injury causes and factors,total burn surface area,inhalation injury and compound injury),the time when each first-aid rescue team was in place;the reception time of the triage group;specialist rescue start time;treatment process and treatment outcome in patients with severe burns,such as the establishment time of deep venous access.Results:67 cases(n=68)of patients were cured and the curative ratio was 98.5%.The average time from the rescue order of the commander-in-chief to the placement of each medical group was 8.8 minutes,the average reception time of the triage group was 4.5 minutes,the average specialist rescue start time was 18.6 minutes,and the average establishment time of deep vein access for patients with severe burns was about 11.3 minutes.Conclusions:Scientific and reasonable pre-hospital emergency triage and treatment for burn patients can be achieved by implementing integrated treatment.展开更多
Objective:This study is designed to analyze the impact of COVID-19 on hospitals in a certain region through the statistical comparison of outpatient,emergency and inpatient data of three representative AAA hospitals i...Objective:This study is designed to analyze the impact of COVID-19 on hospitals in a certain region through the statistical comparison of outpatient,emergency and inpatient data of three representative AAA hospitals in this region from January and February of 2019 and 2020.Methods:Relevant outpatient,emergency and inpatient data of three representative AAA hospitals in a certain region from January and February of 2019 and 2020 were collected,and the statistical analysis was conducted by using EXCEL 2019 and SPSS20.0.Results:In January and February of 2020,the number of the outpatient,the emergency,the inpatient and the inpatient surgical interventions in the three hospitals was significantly decreased in comparison with the same period in 2019,and the change of the emergency dispatching amount was relatively small.The mortality of the inpatient in January and February in 2020 was about 2.82%,and that in the same period in 2019 was about 1.51%,with an increase of about 1.31%,which presented a statistically significant difference(χ^(2)=348.422;p=.000).Conclusions:The outbreak of COVID-19 had a great impact on the normal operation of the hospital.展开更多
At present,the multiple drug resistance of Acinetobacter baumannii outbreaks worldwide and has intensified the trend,especially in the intensive care unit and burn ward.Generic drug resistant Acinetobacter baumannii i...At present,the multiple drug resistance of Acinetobacter baumannii outbreaks worldwide and has intensified the trend,especially in the intensive care unit and burn ward.Generic drug resistant Acinetobacter baumannii is known as the 21st century gram-negative bacterium“MRSA”,“Superbugs”.In recent years,researches have shown that this is associated with pathogenic bacteria to form biofilms.In this paper,the status of Acinetobacter baumannii infection,biofilm formation,resistance mechanism and prevention in recent years were summarized.展开更多
Objective:To discuss thymopentin-5 on renal pathology and relevant indexes of serum with sepsis-caused acute kidney injury(AKI)caused by cecal ligation and puncture(CLP).Methods:90 cases of healthy and male SD rats we...Objective:To discuss thymopentin-5 on renal pathology and relevant indexes of serum with sepsis-caused acute kidney injury(AKI)caused by cecal ligation and puncture(CLP).Methods:90 cases of healthy and male SD rats were randomly divided into 3 groups:control group(C group),sepsis group(S group),thymopentin group(T group).These groups were divided into five time point including 1 h,6 h,12 h,24 h and 48 h with 6 rats in each time point.The sepsis model was made by CLP.The blood and kidney tissue were collected in each time point.Changes of renal pathology were observed under light microscope and relevant indexes like serum creatinin(Cr),blood urea nitrogen(Bun),tumor necrosis factorα(TNF-α),interleukin-10(IL-10),CD4+/CD8+were tested and analyzed.Results:In T group,concentrations of Cr,Bun in 6 h after CLP started to rise,reached peak in 24 h and decreased in 48 h,which were all lower than S group(p<.05)and higher than C group(p<.05).Compared with C group,concentrations of TNF-αin 1 h significantly improved,reached peak in 12 h and decreased in 24 h,which were all lower than S group(p<.05);and concentrations of IL-10 in 1 h significantly decreased,reached peak in 12 h and rised in 24 h,which were all more than S group(p<.05).In T group,CD4+/CD8+ratio in 6 h after CLP started to decrease,reached the lowest in 24 h and rised in 48 h,which were all lower than S group(p<.05)and significantly lower than C group(p<.05).Conclusions:Thymopentin-5 plays the role of renal protection in AKI caused by sepsis.展开更多
Multiple organ dysfunction syndrome is the presence of altered organ function of two or more organ systems in acute ill patients with severe trauma,burn,shock and infection.In this case,the patient with burn area amou...Multiple organ dysfunction syndrome is the presence of altered organ function of two or more organ systems in acute ill patients with severe trauma,burn,shock and infection.In this case,the patient with burn area amounted to 95%and the third-degree burn was up to 90%.He underwent gastrointestinal tract,blood clotting,lung,brain,heart,liver dysfunction,and cardiac arrest for 30 minutes during the courses of treatment,and was discharged from the hospital after 108 days on the basis of comprehensive treatment and repeated skin grafting.展开更多
基金supported in part by the National Key R&D Program of China Grant 2022YFB4602200.
文摘With the intelligent upgrading of manufacturing equipment,achieving high-precision and efficient fault diagnosis is essential to enhance equipment stability and increase productivity.Online monitoring and fault diagnosis technology play a critical role in improving the stability of metal additive manufacturing equipment.However,the limited proportion of fault data during operation challenges the accuracy and efficiency of multi-classification models due to excessive redundant data.A multi-sensor and principal component analysis(PCA)and support vector machine(SVM)asymptotic classification(PCSV)for additive manufacturing fault diagnosis method is proposed,and it divides the fault diagnosis into two steps.In the first step,real-time data are evaluated using the T2 and Q statistical parameters of the PCAmodel to identify potential faults while filtering non-fault data,thereby reducing redundancy and enhancing real-time efficiency.In the second step,the identified fault data are input into the SVM model for precise multi-class classification of fault categories.The PCSV method advances the field by significantly improving diagnostic accuracy and efficiency,achieving an accuracy of 99%,a diagnosis time of 0.65 s,and a training time of 503 s.The experimental results demonstrate the sophistication of the PCSV method for high-precision and high-efficiency fault diagnosis of small fault samples.
基金the support from the National Science and Technology Major Project, China (No. J2019IV-0014-0082)the National Key Research and Development Program of China (No. 2022YFB4600700)+1 种基金the National Overseas Youth Talents Program, China, the Research Fund of State Key Laboratory of Mechanics and Control for Aerospace Structures, China (No. MCMS-I-0422K01)a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China。
文摘Fatigue properties of materials by Additive Manufacturing(AM) depend on many factors such as AM processing parameter, microstructure, residual stress, surface roughness, porosities, post-treatments, etc. Their evaluation inevitably requires these factors combined as many as possible, thus resulting in low efficiency and high cost. In recent years, their assessment by leveraging the power of Machine Learning(ML) has gained increasing attentions. A comprehensive overview on the state-of-the-art progress of applying ML strategies to predict fatigue properties of AM materials, as well as their dependence on AM processing and post-processing parameters such as laser power, scanning speed, layer height, hatch distance, built direction, post-heat temperature,etc., were presented. A few attempts in employing Feedforward Neural Network(FNN), Convolutional Neural Network(CNN), Adaptive Network-Based Fuzzy Inference System(ANFIS), Support Vector Machine(SVM) and Random Forest(RF) to predict fatigue life and RF to predict fatigue crack growth rate are summarized. The ML models for predicting AM materials' fatigue properties are found intrinsically similar to the commonly used ones, but are modified to involve AM features. Finally, an outlook for challenges(i.e., small dataset, multifarious features,overfitting, low interpretability, and unable extension from AM material data to structure life) and potential solutions for the ML prediction of AM materials' fatigue properties is provided.
基金the support by the Office of Naval Research’s NEPTUNE Program under the Grant Number N00014-16-1-3109the National Science Foundation CMMI NanoManufacturing Program。
文摘The remarkable capabilities of 2D plasmonic surfaces in controlling optical waves havegarnered significant attention.However,the challenge of large-scale manufacturing of uniform,well-aligned,and tunable plasmonic surfaces has hindered their industrialization.To address this,we present a groundbreaking tunable plasmonic platform design achieved throughmagnetic field(MF)assisted ultrafast laser direct deposition in air.Through precise control of metal nanoparticles(NPs),with cobalt(Co)serving as the model material,employing an MF,and fine-tuning ultrafast laser parameters,we have effectively converted coarse and non-uniform NPs into densely packed,uniform,and ultrafine NPs(~3 nm).This revolutionary advancement results in the creation of customizable plasmonic‘hot spots,’which play a pivotal role insurface-enhanced Raman spectroscopy(SERS)sensors.The profound impact of this designable plasmonic platform lies in its close association with plasmonic resonance and energyenhancement.When the plasmonic nanostructures resonate with incident light,they generate intense local electromagnetic fields,thus vastly increasing the Raman scattering signal.This enhancement leads to an outstanding 2–18 fold boost in SERS performance and unparalleled sensing sensitivity down to 10^(-10)M.Notably,the plasmonic platform also demonstratesrobustness,retaining its sensing capability even after undergoing 50 cycles of rinsing andre-loading of chemicals.Moreover,this work adheres to green manufacturing standards,making it an efficient and environmentally friendly method for customizing plasmonic‘hot spots’inSERS devices.Our study not only achieves the formation of high-density,uniform,and ultrafine NP arrays on a tunable plasmonic platform but also showcases the profound relation betweenplasmonic resonance and energy enhancement.The outstanding results observed in SERS sensors further emphasize the immense potential of this technology for energy-relatedapplications,including photocatalysis,photovoltaics,and clean water,propelling us closer to a sustainable and cleaner future.
文摘Bacterial biofilms(BFs)are widely present in nature,and more than 99%of bacteria can form BF,which is an important factor leading to persistent infection of refractory wound and repeated infection.The formation of BF is a dynamic cyclic process involving various physical,chemical and biological processes,mainly including bacterial attachment,BF formation and maturation,and bacterial diffusion.The bacteria in bacterial biofilms are more resistant to antibiotics and disinfectants,and more resilient to environmental changes,which presents many challenges in treatment.This article reviews the basic characteristics,resistance mechanisms and treatment strategies of BF.At present,there are many studies on the treatment of BF,which need to be selected according to the specific situation and pathophysiological process of wound infection,and can be used as a single method or in combination.This article introduces some treatment methods to provide a reference for the clinical prevention and treatment of BF.
基金This study was co-supported by the National Natural Science Foundation of China(No.51301090).
文摘The nonlinear cumulative damage model is modified to have high prediction accuracy when the high-low cycle stress frequency ratio m is large(m500).The low cycle fatigue(LCF)tests,high cycle fatigue(HCF)tests and combined high and low cycle fatigue(CCF)tests of TC11 titanium alloy were carried out,and the influencing factors of CCF life were analysed.The CCF life declines with the decrease of the ratio of high-low cycle stress frequency m.Both linear and nonlinear cumulative damage models are used to predict the CCF life.The CCF life prediction error of the linear cumulative damage model is great and the predictions tend to be overestimated,which is dangerous for engineering application.The accuracy is relatively high when the high-low cycle stress frequency ratio m500.The accuracy of nonlinear cumulative damage model is higher than that of linear model when the high-low cycle stress frequency ratio m500.Based on the relationship between high cycle average stress rmajor and material yield limit rp,0.2,a correction term is added to the nonlinear cumulative damage model and verified,which made the modified model more accurate when m500.
文摘Burn as a strong traumatic stimulus can induce inflammatory responses mediated by neuroendocrine,cytokines and other inflammatory mediators,and directly lead to organ dysfunction,resulting in severe MODS.Excessive inflammatory responses caused by necrotic tissues in the burned wound,also play an important role in stimulating the occurrence and the development of MODS.Complete removal of necrotic tissues in the early stage of burns to prevent from invasive wound infections,can effectively prevent MODS.AKI occurred earlier in this case,combined with its admission after the onset of shock and no obstruction of the urinary system can exclude prerenal and renal AKI,speculated as renal AKI.The factors leading to renal AKI are divided into renal ischemia and nephrotoxicity.The patient had adequate urinary output during shock and AKI in the presence of adequate renal blood flow,possibly due to the nephrotoxicity of a large number of necrotic tissues of the wound after they were broken down and absorbed into the bloodstream.The patient fell asleep after being drunk and the bedside stove broiled him for a long time,on one hand,heat accumulation lead to skin damage;on the other hand,sustained heat action caused hyperemia,edema and hemodynamic disorder in the deep tissues,and then resulted in secondary necrosis.
文摘Since the discovery of adipose-derived mesenchymal stem cell (ADSC) in more than ten years, a great progress has been made from its basic research to clinical application. Compared with bone marrow mesenchymal stem cells, ADSC is more abundant in reserve, easier to obtain with fewer injuries and less complications. These cells have multiple differentiation potential and can differentiate into adipocytes, chondrocytes and osteoblasts with the influence of different inducing factors. Early studies of ADSC mainly focused on the ability of multi-directional differentiation, especially on the regeneration of bone defects and cartilage tissue. At present, the researches mainly focus on immunoregulation and paracrine function of ADSC. Although ADSC has made a great progress in clinical application, the cell preparation, use pattern, and mechanisms in clinical treatment are not clear. This paper elaborates on these issues.
文摘China began using the colored liquid from tea and tincture to treat burns as early as the sixth century B.C.,and records of Chinese medicine for the rescue and treatment of burns date from the Jin Dynasty to the Ming and Qing Dynasties.Modern burn treatment medicine in China began in 1958 when national steel production began.Since then,burn patients have increased dramatically,independent burn departments have been established nationwide,and burn surgery has moved into technological development.With the combined efforts of several generations of burn medical workers in China,the clinical treatment of severe and extra-severe burns has steadily increased.Many fields,such as burn shock prevention and treatment,wound repair,and sepsis,have become world leaders in the 21st century,forming a treatment technology and theoretical system with distinctive Chinese characteristics.Burn medical professionals have conducted numerous clinical and fundamental experimental studies,founded the Burns Surgery Branch of the Chinese Medical Association,established academic journals,and successfully established China Burn Treatment Program.The advancement of science and technology has promoted the development of modern medicine,and 3D virtual reality technology,3D printing technology,organoid culture,and tissue engineering skin have demonstrated potential advantages and excellent application prospects in the treatment of severe burns,providing new ideas and directions for the discipline’s development.In the new journey of development and reform,we should continue to strengthen the systematic construction of the discipline,enhance the innovative treatment capability,and create further triumph.
文摘Objective: To explore the effects of combined application of culture supernatant of human umbilical cord mesenchymal stem cells (hUCMSCs) and ciprofloxacin on Staphylococcus aureus (SA) in vitro. Methods: hUCMSCs were isolated from umbilical cord tissues of full-term healthy fetuses after cesarean section and then cultured. Cells in the third passage were chosen for the use of experiment after identification. SA strains isolated from wounds of burn patients in our burn wards were used in the following experiment. Cells were divided into 0, 10, 100 and 1,000 ng/ml lipopolysaccharide (LPS) groups by use of the random number table (similarly hereinafter). Cells were cultured with culture medium containing mesenchymal stem cells (MSCs) after being treated with medium containing corresponding mass concen-trations of LPS for 12 h. At post culture hour (PCH) 6, 12 and 24, 6 wells of culture supernatant of cells in each group were obtained to measure the content of LL-37 with enzyme-linked immunosorbent assay (ELISA). Ninety blood agar culture plates were divided into ciprofloxacin control group (CC), ciprofloxacin + supernatant group (CS), and ciprofloxacin + supernatant+ LL-37 antibody group (CSL), with 30 blood agar culture plates in each group. Blood agar culture plates in group CC were coated with 1.5 × 108 colony forming unit (CFU)/ml bacteria solution prepared with normal saline. Blood agar culture plates in group CS were coated with 1.5 × 108 CFU/ml bacteria solution prepared with normal saline and hUCMSC culture supernatant (cultured by MSC culture medium, the same below) in double volume of normal saline. Blood agar culture plates in group CSL were coated with 1.5 × 108 CFU/ml bacteria solution prepared with normal saline, hUCMSC culture supernatant in double volume of normal saline, and 2.6 μL of LL-37 antibodies at the concentration of 2 μg/ml. At PCH 12, 24 and 48, 10 blood agar culture plates were taken out from each group to observe the distribution of SA colonies on blood agar culture plates and to measure diameters of zones of inhibition of ciprofloxacin. The minimum inhibitory concentration (MIC) of ciprofloxacin against SA in each group was recorded. Fractional inhibitory concentration (FIC) indexes of ciprofloxacin in group CS and group CSL at PCH 12, 24 and 48 were calculated, with the synergistic effect evaluated. Data were processed with factorial design ANOVA, one way ANOVA, LSD-t test, Kruskal-Wallis test and Mann-Whitney U test. Results: (1) At each PCH, the content of LL-37 in cell culture supernatant in 10 ng/ml LPS group, 100 ng/ml LPS group or 1,000 ng/ml LPS group was higher than that in 0 ng/ml LPS group (with t values ranging from 11.22 to 33.36, p values all below .01);the content of LL-37 in cell culture supernatant in either 100 ng/ml LPS group or 1,000 ng/ml LPS group was higher than that in 10 ng/ml LPS group (with t values ranging from 2.24 to 18.73, p < .05 or p < .01);the content of LL-37 in cell culture supernatant in 1,000 ng/ml LPS group was higher than that in 100 ng/ml LPS group (with t values ranging from 12.46 to 14.70, p values all below .01). (2) At PCH 12, 24 and 48, the bacterial colonies in groups CC, CS and CSL became integrated over time. In CC group, diameters of zones of inhibition of ciprofloxacin at PCH 12, 24 and 48 were 26 mm, 24 mm and 23 mm respectively, with no obvious changes. At PCH 12, 24 and 48, diameters of zones of inhibition of ciprofloxacin in groups CS and CSL were 82 mm, 71 mm, 68 mm and 74 mm, 59 mm, 56 mm respectively, which were significantly larger than those in group CC. (3) At each PCH, MIC of ciprofloxacin against SA in group CC was significantly higher than that in groups CS and CSL respectively (with Z values ranging from 6.22 to 6.71, p values all below .01);MIC of ciprofloxacin against SA in group CSL was significantly higher than that in group CS (with Z values all equal to 6.72, p values all below .01). (4) FIC indexes of ciprofloxacin in groups CS and CSL at PCH 12, 24 and 48 were 0.011, 0.032, 0.032 and 0.122, 0.350, 0.350, respectively. The results indicated that hUCMSC culture supernatant had a synergistically antibacterial effect when combined with ciprofloxacin. Conclusions: hUCMSCs can secrete LL-37, and the secretion level is improved with increase of LPS concentration. The com-bination of hUCMSC culture supernatant with ciprofloxacin can decrease the dosage of ciprofloxacin in resisting SA effectively. Once LL-37 is neutralized, the synergistically antibacterial effect of hUCMSC culture supernatant is decreased thereby.
文摘Objective:To observe the protective effects and mechanisms of topical Chinese medicine on kidneys of severe burns shock rats.Methods:Blood urea nitrogen(Bun),creatinine(Cr),myeloperoxidase(MPO),tumor necrosis factor-α(TNF-α)and inter-leukin(IL-6),were checked by the time points of 8,24,48 hours.Blood tests,ELISA and other methods were used for these texts.Results:Compared with the povidone-iodine group,the levels of Bun and Cr were declined apparently in the Sanhuang group,and the activity of MPO was decreased as well.Conclusions:Sanhuangshaoshangling,taking ointment as matrix,can be externally applied to wounds.It contains some ef-fective ingredients which can clear heat-toxicity and promote blood circulation for removing blood stasis,and prevents wounds from the stimulation of the air to nerve endings and the invasion of bacteria.It can also inhibit bacteria from parasitizing,grow-ing,reproducing on the wound.Sanhuangshaoshangling helps blood circulation and facilitates the growth of tissues to inactivate the toxicity or reduce toxin by changing the structure of endotoxin after uptake into tissues.It can protect viscera by reducing the release of endotoxin,facilitating the excretion of endotoxin and weakening or eliminating the effects of TNF-αand IL-6.
基金This work was supported by the found from National Natural Science Foundation of China(82060348)Inner Mengolia Science and Technology Plan of Inner Mengolia of China(2020GG0214)+2 种基金National Natural Science Foundation of Inner Mongolia(2021MS08066)Joint Research Found of Inner Mongolia Medical University(YKD2021LH055)Sponsored by Science Foundation of AMHT(2022YK16).
文摘Cell therapy represents an important area of biotechnology,with the potential to treat and even cure a variety of diseases that cannot be cured by traditional therapies.Cell therapy is currently a widely studied treatment in both preclinical and clinical settings,and many cell therapy products are undergoing clinical trials.This article reviews the application of cell therapy in a variety of diseases and the policy of stem cell therapy in China.
文摘Objective: To explore the significance of D-dimer assay in the diagnosis of acute pulmonary embolism (APE) after deep second-degree burn by detecting the changes of plasma D-dimer in rabbits with deep second-degree burn and in different phases of burn with concomitant pulmonary embolism. Methods: 48 healthy male or female Chinese white rabbits, were randomly divided into: control group, burn group, burn shock group, burn shock + pulmonary embolism group, burn infection group and burn infection + pulmonary embolism group, with 8 rabbits in each group. By use of the ELISA method, the plasma D-dimer was determined on 1 d, 3 d, 5 d, 7 d, 14 d and 21 d after modeling in the burn group and the control group, and the plasma D-dimer in other groups was detected in 30 min, 1 h, 3 h, 6 h, 24 h after modeling. Results: The expression of D-dimer in the burn group was higher than that in the control group on 1 d, 3 d, 5 d and 7 d (p < .05). There were significant differences in the expression of D-dimer in the burn shock + pulmonary embolism group in comparison to the burn shock group (p < .05). There were also some significant differences in the expression of D-dimer in the burn infection+ pulmonary embolism group in comparison with the burn shock + pulmonary embolism group (p < .05). Conclusions: There were some differences in the expression of D-dimer in each group. D-dimer can be used as one of diagnostic indexes for deep second-degree burn with pulmonary embolism.
文摘Objective:To explore the potential protective effects of ulinastatin on ventilation-induced lung injuries of severe burned rats.Methods:Ninety Wistar rats were randomly divided into three experimental groups:the control group(n=30),the ventilation group(n=30)and the ventilation-ulinastatin group(n=30).After establishing the severe burn model,the rats of latter two groups were mechanically ventilated for 1 hour with or without the pre-treatment of ulinastatin.After severe scald,the protective effect of ulinastatin on lung injury caused by mechanical ventilation was estimated through the observation of the tissues samples,and evaluation of the pathological changes of lung tissue by HE staining,ultrastructure change by electron microscopy,lung coefficient,and the expression levels of lung tissue cytokines TNF-α,IFN-γ,IL-2 by immunohistochemical staining.Results:Edema in lung tissues of the control group and the ventilation group was obvious,the hemorrhagic focus could be seen,and the cut surface was observed to be scattered and swelling;Edema in lung tissues of the ventilation-ulinastatin group was mild.HE staining revealed that the pathological changes of the ventilation-ulinastatin group were milder than the ventilation group.Under the electron microscope,the lung tissue organelles of the control group and the ventilation group were seriously damaged;the corresponding changes in the ventilation-ulinastatin group were lighter.The lung coefficient of the ventilation-ulinastatin group was significantly lower than that in the ventilation group.The immunohistochemical results showed that the intensity of TNF-α,IL-2 and IFN-γin lung tissue of the ventilation-ulinastatin group was significantly lower than that in the ventilation group.Conclusions:Ulinastatin has protective effects on lung injury caused by mechanical ventilation in severe scalded rats,whose mechanism may be related to the capacity of ulinastatin to reduce the expression of cytokines including TNF-α,IL-2 and IFN-γ.
文摘Objective:To investigate the expression of cycloxygenase-2(COX-2),and vascular endothelial growth factor(VEGF)by using immunohistochemistry method and observe the changes of histological structure between the all skin group and the xenoskin(sheepskin)group,and explore the effect of the xenoskin(sheepskin)which is a kind of covering of autogenous microskin grafting.Methods:A total of 180 Wistar healthy male rats were randomly divided into 3 groups:the autoskin group(the control group),the alloskin group and the xenoskin group(the sheepskin group),with 60 rats in each group.By using immunohistochemistry method,the expression of COX-2 and VEGF were revealed on 3,5,7,14,21 and 28 d,with 10 executed rats in each group on each time points,and the structure of histology was observed by light microscope.Results:There were obvious difference of expression of COX-2 and VEGF on wound in the autoskin group compared with other two groups(p<.01);but there were no differences between the alloskin group and xenoskin group(p>.05).Observed on light microscope,the paralleled collagen and elastic fiber appeared in the autoskin group on 14 post-transplantation days,but they appeared in the alloskin group and the xenoskin group only on 21 post-transplantation days.Conclusions:There were no differences of the expression of COX-2 and VEGF in the alloskin group and the xenoskin group,which provided clinical theoretical basis on sheepskin as a substitute for the alloskin to cover autogenous microskin.
文摘Inflammation is generally considered as a pathological process,and the occurrence of multi-organ inflammatory diseases is usually accompanied by an inflammatory response.Persistent inflammation can eventually lead to organ dysfunction.In recent years,the study of mesenchymal stem cell-derived exosomes has found its unique anti-inflammatory effect,which provides a new idea for the treatment of multi-organ inflammatory diseases.This article reviews the effect of mesenchymal stem cell exosomes on multi-organ inflammatory diseases.
基金Health Research Project of Metallurgical Safety and Health Branch of The Chinese Society for Metals(JKWS201832)Natural Science Foundation of Inner Mongolia Autonomous Region(2020MS03022)+1 种基金Health Science and Technology Project of Inner Mongolia Autonomous Region(202201523)Joint Project of Inner Mongolia Medical University(YKD2021LH057).
文摘Objective:To explore the burn treatment and first aid flow mode in batch patients.Methods:A retrospective analysis was performed on the treatment experience of 10 or more patients in the group burn accident admitted to the Third Affiliated Hospital of Inner Mongolia Medical University from 2008 to 2020,in order to acquire the general information(e.g.,age,sex[male and female],injury causes and factors,total burn surface area,inhalation injury and compound injury),the time when each first-aid rescue team was in place;the reception time of the triage group;specialist rescue start time;treatment process and treatment outcome in patients with severe burns,such as the establishment time of deep venous access.Results:67 cases(n=68)of patients were cured and the curative ratio was 98.5%.The average time from the rescue order of the commander-in-chief to the placement of each medical group was 8.8 minutes,the average reception time of the triage group was 4.5 minutes,the average specialist rescue start time was 18.6 minutes,and the average establishment time of deep vein access for patients with severe burns was about 11.3 minutes.Conclusions:Scientific and reasonable pre-hospital emergency triage and treatment for burn patients can be achieved by implementing integrated treatment.
文摘Objective:This study is designed to analyze the impact of COVID-19 on hospitals in a certain region through the statistical comparison of outpatient,emergency and inpatient data of three representative AAA hospitals in this region from January and February of 2019 and 2020.Methods:Relevant outpatient,emergency and inpatient data of three representative AAA hospitals in a certain region from January and February of 2019 and 2020 were collected,and the statistical analysis was conducted by using EXCEL 2019 and SPSS20.0.Results:In January and February of 2020,the number of the outpatient,the emergency,the inpatient and the inpatient surgical interventions in the three hospitals was significantly decreased in comparison with the same period in 2019,and the change of the emergency dispatching amount was relatively small.The mortality of the inpatient in January and February in 2020 was about 2.82%,and that in the same period in 2019 was about 1.51%,with an increase of about 1.31%,which presented a statistically significant difference(χ^(2)=348.422;p=.000).Conclusions:The outbreak of COVID-19 had a great impact on the normal operation of the hospital.
文摘At present,the multiple drug resistance of Acinetobacter baumannii outbreaks worldwide and has intensified the trend,especially in the intensive care unit and burn ward.Generic drug resistant Acinetobacter baumannii is known as the 21st century gram-negative bacterium“MRSA”,“Superbugs”.In recent years,researches have shown that this is associated with pathogenic bacteria to form biofilms.In this paper,the status of Acinetobacter baumannii infection,biofilm formation,resistance mechanism and prevention in recent years were summarized.
文摘Objective:To discuss thymopentin-5 on renal pathology and relevant indexes of serum with sepsis-caused acute kidney injury(AKI)caused by cecal ligation and puncture(CLP).Methods:90 cases of healthy and male SD rats were randomly divided into 3 groups:control group(C group),sepsis group(S group),thymopentin group(T group).These groups were divided into five time point including 1 h,6 h,12 h,24 h and 48 h with 6 rats in each time point.The sepsis model was made by CLP.The blood and kidney tissue were collected in each time point.Changes of renal pathology were observed under light microscope and relevant indexes like serum creatinin(Cr),blood urea nitrogen(Bun),tumor necrosis factorα(TNF-α),interleukin-10(IL-10),CD4+/CD8+were tested and analyzed.Results:In T group,concentrations of Cr,Bun in 6 h after CLP started to rise,reached peak in 24 h and decreased in 48 h,which were all lower than S group(p<.05)and higher than C group(p<.05).Compared with C group,concentrations of TNF-αin 1 h significantly improved,reached peak in 12 h and decreased in 24 h,which were all lower than S group(p<.05);and concentrations of IL-10 in 1 h significantly decreased,reached peak in 12 h and rised in 24 h,which were all more than S group(p<.05).In T group,CD4+/CD8+ratio in 6 h after CLP started to decrease,reached the lowest in 24 h and rised in 48 h,which were all lower than S group(p<.05)and significantly lower than C group(p<.05).Conclusions:Thymopentin-5 plays the role of renal protection in AKI caused by sepsis.
文摘Multiple organ dysfunction syndrome is the presence of altered organ function of two or more organ systems in acute ill patients with severe trauma,burn,shock and infection.In this case,the patient with burn area amounted to 95%and the third-degree burn was up to 90%.He underwent gastrointestinal tract,blood clotting,lung,brain,heart,liver dysfunction,and cardiac arrest for 30 minutes during the courses of treatment,and was discharged from the hospital after 108 days on the basis of comprehensive treatment and repeated skin grafting.
