Alterations in the mesenchymal-epithelial transition factor(MET)gene are critical drivers of non-small cell lung cancer(NSCLC).In recent years advances in precision therapies targeting MET alterations have significant...Alterations in the mesenchymal-epithelial transition factor(MET)gene are critical drivers of non-small cell lung cancer(NSCLC).In recent years advances in precision therapies targeting MET alterations have significantly expanded treatment options for NSCLC patients.These alterations include MET exon 14 skipping mutations(MET exon 14 skipping),MET gene amplifications,MET point mutations(primarily kinase domain mutations),and MET protein overexpression.Accurate identification of these alterations and appropriate selection of patient populations and targeted therapies are essential for improving clinical outcomes.The East China Lung Cancer Group,Youth Committee(ECLUNG YOUNG,Yangtze River Delta Lung Cancer Cooperation Group)has synthesized insights from China’s innovative drug development landscape and clinical practice to formulate an expert consensus on the diagnosis and treatment of NSCLC patients with MET alterations.This consensus addresses key areas,such as optimal testing timing,testing methods,testing strategies,quality control measures,and treatment approaches.By offering standardized recommendations,this guidance aims to streamline diagnostic and therapeutic processes and enhance clinical decision-making for NSCLC with MET alterations.展开更多
Ultrasensitive detection of multiple diseases markers is of great importance in improving diagnostic accuracy,precision,and efficiency.A versatile Au nanozyme Raman probe strategy was employed to develop an ultrasensi...Ultrasensitive detection of multiple diseases markers is of great importance in improving diagnostic accuracy,precision,and efficiency.A versatile Au nanozyme Raman probe strategy was employed to develop an ultrasensitive multiplex surface-enhanced Raman scattering(SERS)immunosensor using encoded silica photonic crystal beads(SPCBs).The efficient Au nanozyme Raman probe strategy was constructed using a robust Au nanozyme with high dual enzyme-like activity and SERS activity.On the one hand,Au nanozyme tags with oxidase-like activity can catalyze the oxidation of Raman-inactive 3,3,5,5-tetramethylbenzidine(TMB)to Raman-active oxidized TMB(ox-TMB)in the presence of O_(2).On the other hand,Au nanozyme tags with peroxidase-like activity can catalyze Raman-inactive TMB to Ramanactive ox-TMB in the presence of H_(2)O_(2).This dual catalysis action results in many Raman-active reporter molecules(ox-TMB)enabling highly sensitive detection.Meanwhile,the Au nanozyme as an extraordinary SERS substrate further enhances the detection signals of these Raman reporter molecules.Using reflection peaks of different SPCBs to encode tumor markers,an ultrasensitive multiplex SERS immunosensor was developed for detection of carcinoembryonic antigen(CEA)and alpha-fetoprotein(AFP),which exhibited wide linear ranges of 0.001-100 ng/m L for CEA and 0.01-1000 ng/m L for AFP,accompanied by low detection limits of 0.66 pg/m L for CEA and 9.5 pg/m L for AFP,respectively.This work demonstrates a universal and promising nanozyme Raman probe strategy to develop ultrasensitive multiplex SERS immunosensors for precise clinical diagnosis of disease.展开更多
Although diverse signal-amplified methods have been committed to improve the sensitivity of surface plasmon resonance(SPR)biosensing,introducing convenient and robust signal amplification strategy into SPR biosensing ...Although diverse signal-amplified methods have been committed to improve the sensitivity of surface plasmon resonance(SPR)biosensing,introducing convenient and robust signal amplification strategy into SPR biosensing remains challenging.Here,a novel nanozyme-triggered polymerization amplification strategy was proposed for constructing highly sensitive surface plasmon resonance(SPR)immunosensor.In detail,Au@Pd core-shell nanooctahedra nanozyme with superior peroxidase(POD)-like activity was synthesized and utilized as a label probe.Simultaneously,Au@Pd core-shell nanooctahedra nanozyme can catalyze the decomposition of H_(2)O_(2)to form hydroxyl radicals(·OH)that triggers the polymerization of aniline to form polyaniline attaching on the surface of sensor chip,significantly amplifying SPR responses.The sensitivity of SPR immunosensor was enhanced by nanozyme-triggered polymerization amplification strategy.Using human immunoglobulin G(HIgG)as a model,the constructed SPR immunosensor obtains a wide linear range of 0.005–1.0μg/m L with low detection limit of 0.106 ng/m L.This research provides new sights on establishing sensitive SPR immunosensor and may evokes more inspiration for developing signal amplification methods based on nanozyme in biosensing.展开更多
3 D highly ordered silver nanoparticles(AgNPs) coated silica photonic crystal beads(Ag/SPCBs) were prepared and exploited as a novel surface enhanced Raman scattering(SERS) substrate.The monodisperse and size-controll...3 D highly ordered silver nanoparticles(AgNPs) coated silica photonic crystal beads(Ag/SPCBs) were prepared and exploited as a novel surface enhanced Raman scattering(SERS) substrate.The monodisperse and size-controlled SPCBs were prepared via self-assembly of silica nanoparticles process using a simple microfluidic device.Then the Ag/SPCBs were easily obtained by in situ growth of AgNPs onto the NH_(2)-modified SPCBs.Field emitting scanning electron microscopy(SEM) and energy dispersive X-ray spectrometry(EDX) were used to characterize the Ag/SPCBs.The effect of silica nanoparticle size and AgNO_(3) concentration on the SERS performance of the resultant Ag/SPCBs substrate were discussed in detail.The results indicate that the Ag/SPCBs have highest SERS signals when silica nanoparticle size is250 nm and AgNO_(3) concentration is 0.8 mg/mL.Using malachite green(MG) as model analyte,the Ag/SPCBs substrate displayed a high sensitivity and a wide linear range for MG.The well-designed Ag/SPCBs show high uniformity and excellent reproducibility,and can be used as an effective SERS substrate for sensitive assay application.展开更多
As an emerging biomarker,tumor mutational burden(TMB)has attracted increasing attention from clinicians in predicting the efficacy of tumor immunotherapy.Currently,TMB is detected primarily by whole-exome sequencing o...As an emerging biomarker,tumor mutational burden(TMB)has attracted increasing attention from clinicians in predicting the efficacy of tumor immunotherapy.Currently,TMB is detected primarily by whole-exome sequencing or targeted panel sequencing on high-throughput sequencing platforms.However,the lack of uniformity in detection methods,threshold settings,and reporting formats,as well as the significant differences in TMB values among different cancer types,have hindered the standardized application of this biomarker in clinical practice.This consensus focuses on the definition,standardization of detection,clinical significance,and limitations of TMB,and provides consensus recommendations for the clinical application of TMB in real-world practice in China.This consensus is aimed at helping clinicians and laboratory personnel understand the clinical significance and testing standards of TMB,promoting more accurate interpretation of test results,and improving patient care.展开更多
The urgency of early lung cancer(LC)diagnosis and treatment has been more and more significant.Exhaled breath analysis using gas sensors is a promising way to find out if someone has LC due to its low-cost,non-invasiv...The urgency of early lung cancer(LC)diagnosis and treatment has been more and more significant.Exhaled breath analysis using gas sensors is a promising way to find out if someone has LC due to its low-cost,non-invasive,and real-time monitoring compared with traditional invasive diagnostic techniques.Among sensor-based gas detection techniques,metal oxide semiconductor’s gas sensors are one of the most important types.This review presents the-state-of-art in metal oxide gas sensors for the diagnosis of early LC.First,the exhaled breath biomarkers are described with emphasis on the concentration of abnormal volatile organic compounds(VOCs)caused by the metabolic process of LC cells.Then,the research status of metal oxide gas sensors in LC diagnosis is summarized.The sensing performance and enhancement strategy of biomarkers provided by metal oxide semiconductor materials are reviewed.Another effective way to improve VOC detection performance is to build a gas sensor array.At the same time,various gas sensors combined with self-powered techniques are mentioned to display a broad development prospect in breath diagnosis.Finally,metal oxide gas sensor-based LC diagnosis is prospected.展开更多
The BRAF gene is an important signaling molecule in human cells that is involved in the regulation of cell growth,differentiation,and survival.When the BRAF gene mutates,it can lead to abnormal activation of the signa...The BRAF gene is an important signaling molecule in human cells that is involved in the regulation of cell growth,differentiation,and survival.When the BRAF gene mutates,it can lead to abnormal activation of the signaling pathway,which promotes cell proliferation,inhibits cell apoptosis,and ultimately contributes to the occurrence and development of cancer.BRAF mutations are widely present in various cancers,including malignant melanoma,thyroid cancer,colorectal cancer,non-small cell lung cancer,and hairy cell leukemia,among others.BRAF is an important target for the treatment of various solid tumors,and targeted combination therapies,represented by BRAF inhibitors,have become one of the main treatment modalities for a variety of BRAF-mutation-positive solid tumors.展开更多
1(NUTM1)gene rearrangements(15q14).In 1991,two independent research teams reported NC cases characterized by the t(15;19)translo-cation.1,2 In vitro studies by French et al.3 led to the pivotal discovery of NC in 2003...1(NUTM1)gene rearrangements(15q14).In 1991,two independent research teams reported NC cases characterized by the t(15;19)translo-cation.1,2 In vitro studies by French et al.3 led to the pivotal discovery of NC in 2003 as a distinct disease entity driven by the fusion of bromodo-main and extraterminal domain(BET)protein 4(BRD4)and NUTM1.In 2004,the World Health Organization(WHO)classified tumors with t(15;19)translocation as a thymic malignancy and designated it“NUT midline carcinoma,”due to its predominant occurrence in midline organs.4 However,subsequent reports revealed NC’s emergence in numerous nonmidline organs,leading to its reclassification as the independent entity“NUT carcinoma of the thorax”by the WHO in 2015.5 NC exhibits rapid progression and profound resistance to conventional radiotherapy and chemotherapy.展开更多
文摘Alterations in the mesenchymal-epithelial transition factor(MET)gene are critical drivers of non-small cell lung cancer(NSCLC).In recent years advances in precision therapies targeting MET alterations have significantly expanded treatment options for NSCLC patients.These alterations include MET exon 14 skipping mutations(MET exon 14 skipping),MET gene amplifications,MET point mutations(primarily kinase domain mutations),and MET protein overexpression.Accurate identification of these alterations and appropriate selection of patient populations and targeted therapies are essential for improving clinical outcomes.The East China Lung Cancer Group,Youth Committee(ECLUNG YOUNG,Yangtze River Delta Lung Cancer Cooperation Group)has synthesized insights from China’s innovative drug development landscape and clinical practice to formulate an expert consensus on the diagnosis and treatment of NSCLC patients with MET alterations.This consensus addresses key areas,such as optimal testing timing,testing methods,testing strategies,quality control measures,and treatment approaches.By offering standardized recommendations,this guidance aims to streamline diagnostic and therapeutic processes and enhance clinical decision-making for NSCLC with MET alterations.
基金financially supported by National Natural Science Foundation of China(Nos.21475116,21575125 and 22474124)the National Natural Science Foundation of Jiangsu Province(Nos.BK20221370,BK20211362)+5 种基金Key University Natural Science Foundation of Jiangsu-Province(No.20KJA150004)the Project for Science and Technology of Yangzhou(No.YZ2022074)the Project for Yangzhou City and Yangzhou University corporation(No.YZ2023204)Cross cooperation project of Subei Peoples’Hospital of Jiangsu Province(No.SBJC220009)the Open Research Fund of State Key Laboratory of Analytical Chemistry for Life Science(No.SKLACLS2405)Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX24_3728)。
文摘Ultrasensitive detection of multiple diseases markers is of great importance in improving diagnostic accuracy,precision,and efficiency.A versatile Au nanozyme Raman probe strategy was employed to develop an ultrasensitive multiplex surface-enhanced Raman scattering(SERS)immunosensor using encoded silica photonic crystal beads(SPCBs).The efficient Au nanozyme Raman probe strategy was constructed using a robust Au nanozyme with high dual enzyme-like activity and SERS activity.On the one hand,Au nanozyme tags with oxidase-like activity can catalyze the oxidation of Raman-inactive 3,3,5,5-tetramethylbenzidine(TMB)to Raman-active oxidized TMB(ox-TMB)in the presence of O_(2).On the other hand,Au nanozyme tags with peroxidase-like activity can catalyze Raman-inactive TMB to Ramanactive ox-TMB in the presence of H_(2)O_(2).This dual catalysis action results in many Raman-active reporter molecules(ox-TMB)enabling highly sensitive detection.Meanwhile,the Au nanozyme as an extraordinary SERS substrate further enhances the detection signals of these Raman reporter molecules.Using reflection peaks of different SPCBs to encode tumor markers,an ultrasensitive multiplex SERS immunosensor was developed for detection of carcinoembryonic antigen(CEA)and alpha-fetoprotein(AFP),which exhibited wide linear ranges of 0.001-100 ng/m L for CEA and 0.01-1000 ng/m L for AFP,accompanied by low detection limits of 0.66 pg/m L for CEA and 9.5 pg/m L for AFP,respectively.This work demonstrates a universal and promising nanozyme Raman probe strategy to develop ultrasensitive multiplex SERS immunosensors for precise clinical diagnosis of disease.
基金supported by National Natural Science Foundation of China(Nos.22474124,21575125)the National Natural Science Foundation of Jiangsu Province(No.BK20221370)+4 种基金Key University Natural Science Foundation of Jiangsu-Province(No.20KJA150004)the Project for Science and Technology of Yangzhou(No.YZ2022074)Project for Yangzhou City and Yangzhou University corporation(No.YZ2023204)the Open Research Fund of State Key Laboratory of Analytical Chemistry for Life Science(No.SKLACLS2405)Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX22_3462)。
文摘Although diverse signal-amplified methods have been committed to improve the sensitivity of surface plasmon resonance(SPR)biosensing,introducing convenient and robust signal amplification strategy into SPR biosensing remains challenging.Here,a novel nanozyme-triggered polymerization amplification strategy was proposed for constructing highly sensitive surface plasmon resonance(SPR)immunosensor.In detail,Au@Pd core-shell nanooctahedra nanozyme with superior peroxidase(POD)-like activity was synthesized and utilized as a label probe.Simultaneously,Au@Pd core-shell nanooctahedra nanozyme can catalyze the decomposition of H_(2)O_(2)to form hydroxyl radicals(·OH)that triggers the polymerization of aniline to form polyaniline attaching on the surface of sensor chip,significantly amplifying SPR responses.The sensitivity of SPR immunosensor was enhanced by nanozyme-triggered polymerization amplification strategy.Using human immunoglobulin G(HIgG)as a model,the constructed SPR immunosensor obtains a wide linear range of 0.005–1.0μg/m L with low detection limit of 0.106 ng/m L.This research provides new sights on establishing sensitive SPR immunosensor and may evokes more inspiration for developing signal amplification methods based on nanozyme in biosensing.
基金the financial support from National Natural Science Foundation of China(Nos.21575125,81870033 and 21475116)333 Project and Qinglan Project of Jiangsu Province,Natural Science Foundation of Jiangsu Province(No.BK20191434)+4 种基金Six Talent Peaks Project of Jiangsu Province for Zhanjun Yang and Juan LiHigh-end Talent Support Program of Yangzhou University for Zhanjun Yang and Juan LiPriority Academic Program Development of Jiangsu Higher Education Institution(PAPD)Project for Science and Technology of Yangzhou(No.YZ2020068)Open Research Fund of State Key Laboratory of Analytical Chemistry for Life Science(No.SKLACLS1915)。
文摘3 D highly ordered silver nanoparticles(AgNPs) coated silica photonic crystal beads(Ag/SPCBs) were prepared and exploited as a novel surface enhanced Raman scattering(SERS) substrate.The monodisperse and size-controlled SPCBs were prepared via self-assembly of silica nanoparticles process using a simple microfluidic device.Then the Ag/SPCBs were easily obtained by in situ growth of AgNPs onto the NH_(2)-modified SPCBs.Field emitting scanning electron microscopy(SEM) and energy dispersive X-ray spectrometry(EDX) were used to characterize the Ag/SPCBs.The effect of silica nanoparticle size and AgNO_(3) concentration on the SERS performance of the resultant Ag/SPCBs substrate were discussed in detail.The results indicate that the Ag/SPCBs have highest SERS signals when silica nanoparticle size is250 nm and AgNO_(3) concentration is 0.8 mg/mL.Using malachite green(MG) as model analyte,the Ag/SPCBs substrate displayed a high sensitivity and a wide linear range for MG.The well-designed Ag/SPCBs show high uniformity and excellent reproducibility,and can be used as an effective SERS substrate for sensitive assay application.
基金supported by grants from the National Natural Science Foundation of China(Grant No.82072577)from the International Medical Association(Grant No.IMA-F-2025-001).
文摘As an emerging biomarker,tumor mutational burden(TMB)has attracted increasing attention from clinicians in predicting the efficacy of tumor immunotherapy.Currently,TMB is detected primarily by whole-exome sequencing or targeted panel sequencing on high-throughput sequencing platforms.However,the lack of uniformity in detection methods,threshold settings,and reporting formats,as well as the significant differences in TMB values among different cancer types,have hindered the standardized application of this biomarker in clinical practice.This consensus focuses on the definition,standardization of detection,clinical significance,and limitations of TMB,and provides consensus recommendations for the clinical application of TMB in real-world practice in China.This consensus is aimed at helping clinicians and laboratory personnel understand the clinical significance and testing standards of TMB,promoting more accurate interpretation of test results,and improving patient care.
基金supported by the Outstanding Youth Foundation of Jiangsu Province of China under Grant No.BK20211548the National Natural Science Foundation of China under Grant No.51872254+1 种基金the Yangzhou City-Yangzhou University Cooperation Foundation under Grant No.YZ2021153the Walloon Region of Belgium through the Interreg V France-Wallonie-Vlaanderen program under PATHACOV project(Grant No.1.1.297).
文摘The urgency of early lung cancer(LC)diagnosis and treatment has been more and more significant.Exhaled breath analysis using gas sensors is a promising way to find out if someone has LC due to its low-cost,non-invasive,and real-time monitoring compared with traditional invasive diagnostic techniques.Among sensor-based gas detection techniques,metal oxide semiconductor’s gas sensors are one of the most important types.This review presents the-state-of-art in metal oxide gas sensors for the diagnosis of early LC.First,the exhaled breath biomarkers are described with emphasis on the concentration of abnormal volatile organic compounds(VOCs)caused by the metabolic process of LC cells.Then,the research status of metal oxide gas sensors in LC diagnosis is summarized.The sensing performance and enhancement strategy of biomarkers provided by metal oxide semiconductor materials are reviewed.Another effective way to improve VOC detection performance is to build a gas sensor array.At the same time,various gas sensors combined with self-powered techniques are mentioned to display a broad development prospect in breath diagnosis.Finally,metal oxide gas sensor-based LC diagnosis is prospected.
基金supported by the Natural Science Foundation of China(grant number 82002456)China Postdoctoral Science Foundation(grant number 2022M723207)+10 种基金the Medical Scientific Research Foundation of Zhejiang Province,China(grant number 2023KY666)Zhejiang Traditional Chinese Medicine Science Fund Project(grant number 2024ZL372)Qiantang Cross Fund Project(grant number 2023-16)National Natural Science Foundation of China of Zhejiang Cancer Hospital Cultivation Project(grant number PY2023006)the Medical Scientific Research Foundation of Zhejiang Province,China(grant number 2024KY812)the Natural Science Foundation of Zhejiang Province(grant number LQ24H160036)Beijing Health Technologies Promotion Program[grant number BHTPP2022041]Peking University Clinical Scientist Training Program and the Fundamental Research Funds for the Central Universities[grant number BMU2024PYJH010]Science Foundation of Peking University Cancer Hospital[grant number PY202333]the Beijing Natural Science Foundation[grant number 7232248]Beijing Hospitals Authority Youth Programme[grant number QML20231902].
文摘The BRAF gene is an important signaling molecule in human cells that is involved in the regulation of cell growth,differentiation,and survival.When the BRAF gene mutates,it can lead to abnormal activation of the signaling pathway,which promotes cell proliferation,inhibits cell apoptosis,and ultimately contributes to the occurrence and development of cancer.BRAF mutations are widely present in various cancers,including malignant melanoma,thyroid cancer,colorectal cancer,non-small cell lung cancer,and hairy cell leukemia,among others.BRAF is an important target for the treatment of various solid tumors,and targeted combination therapies,represented by BRAF inhibitors,have become one of the main treatment modalities for a variety of BRAF-mutation-positive solid tumors.
基金supported by the China Postdoctoral Science Foundation(grant number 2022M723207)the Medical Scientific Research Foundation of Zhejiang Province,China(grant number 2023KY666)+8 种基金Zhejiang Traditional Chinese Medicine Science Fund Project(grant number 2024ZL372)Qiantang Cross Fund Project(grant number 2023-16)the National Natural Science Foundation of China of Zhejiang Cancer Hospital Cultivation Project(grant number PY2023006)the Medical Scientific Research Foundation of Zhejiang Province,China(grant number 2024KY812)the Natural Science Foundation of Zhejiang Province(grant number Q24H160110)the National Natural Science Foundation of China(grant number NSFC82371851)the Science and Technology Foundation of Guizhou Province(Outstanding Young Scientists of Guizhou Province)(grant number Qiankeherencai-YQK(2023)021)the Science and Technology Foundation of Guizhou Province(grant number Qiankehejichu-ZK(2023)General 212)the Science and Technology Foundation of Guizhou Province(grant number Qiankehechengguo-LC(2025)General 068).
文摘1(NUTM1)gene rearrangements(15q14).In 1991,two independent research teams reported NC cases characterized by the t(15;19)translo-cation.1,2 In vitro studies by French et al.3 led to the pivotal discovery of NC in 2003 as a distinct disease entity driven by the fusion of bromodo-main and extraterminal domain(BET)protein 4(BRD4)and NUTM1.In 2004,the World Health Organization(WHO)classified tumors with t(15;19)translocation as a thymic malignancy and designated it“NUT midline carcinoma,”due to its predominant occurrence in midline organs.4 However,subsequent reports revealed NC’s emergence in numerous nonmidline organs,leading to its reclassification as the independent entity“NUT carcinoma of the thorax”by the WHO in 2015.5 NC exhibits rapid progression and profound resistance to conventional radiotherapy and chemotherapy.
