The ground-floor economy has a long history as a significant part of the informal economy.Due to the dependence on its own social status and relationship to the government’s political and economic objectives,it has d...The ground-floor economy has a long history as a significant part of the informal economy.Due to the dependence on its own social status and relationship to the government’s political and economic objectives,it has developed precariously in recent years.In the face of post-epidemic problems,a shortcut is to learn from international experience.This paper used the structural theory and drew from the secondary data,demonstrating the background of informal economy and exploring the rational ways to maintain and develop street vending.Spatialization,legalization and network digitization are proven international approaches,which display the empirical and theoretical implications to urban practice and studies.展开更多
Non-line-of-sight[NLOS]imaging enables the detection and reconstruction of hidden objects around corners,offering promising applications in autonomous driving,remote sensing,and medical diagnosis.However,existing stea...Non-line-of-sight[NLOS]imaging enables the detection and reconstruction of hidden objects around corners,offering promising applications in autonomous driving,remote sensing,and medical diagnosis.However,existing steady-state NLOS imaging methods face challenges in achieving high efficiency and precision due to the need for multiple diffuse reflections and incomplete Fourier amplitude sampling.This study proposes,to our knowledge,a novel steady-state NLOS imaging technique via polarization differential correlography[PDC-NLOS].By employing the polarization difference of the laser speckle,the method designs a single-shot polarized speckle illumination strategy.The fast and stable real-time encoding for hidden objects ensures stable imaging quality of the PDC-NLOS system.The proposed method demonstrates millimeter-level imaging resolution when imaging horizontally and vertically striped objects.展开更多
Non-line-of-sight(NLOS)imaging is a challenging task aimed at reconstructing objects outside the direct view of the observer.Nevertheless,traditional NLOS imaging methods typically rely on intricate and costly equipme...Non-line-of-sight(NLOS)imaging is a challenging task aimed at reconstructing objects outside the direct view of the observer.Nevertheless,traditional NLOS imaging methods typically rely on intricate and costly equipment to scan and sample the hidden object.These methods often suffer from restricted imaging resolution and require high system stability.Herein,we propose a single-shot high-resolution NLOS imaging method via chromato-axial differential correlography,which adopts low-cost continuous-wave lasers and a conventional camera.By leveraging the uncorrelated laser speckle patterns along the chromato-axis,this method can reconstruct hidden objects of diverse complexity using only one exposure measurement.The achieved background stability through singleshot acquisition,along with the inherent information redundancy in the chromato-axial differential speckles,enhances the robustness of the system against vibration and colored stain interference.This approach overcomes the limitations of conventional methods by simplifying the sampling process,improving system stability,and achieving enhanced imaging resolution using available equipment.This work serves as a valuable reference for the real-time development and practical implementation of NLOS imaging.展开更多
Induction of cancer cell ferroptosis has been proposed as a potential treatment in several cancer types.Tumor-associated macrophages(TAMs)play a key role in promoting tumor malignant progression and therapy resistance...Induction of cancer cell ferroptosis has been proposed as a potential treatment in several cancer types.Tumor-associated macrophages(TAMs)play a key role in promoting tumor malignant progression and therapy resistance.However,the roles and mechanisms of TAMs in regulating tumor ferroptosis is still unexplored and remains enigmatic.This study shows ferroptosis inducers has shown therapeutic outcomes in cervical cancer in vitro and in vivo.TAMs have been found to suppress cervical cancer cells ferroptosis.Mechanistically,macrophage-derived miRNA-660-5p packaged into exosomes are transported into cancer cells.In cancer cells,miRNA-660-5p attenuates ALOX15 expression to inhibit ferroptosis.Moreover,the upregulation of miRNA-660-5p in macrophages depends on autocrine IL4/IL13-activated STAT6 pathway.Importantly,in clinical cervical cancer cases,ALOX15 is negatively associated with macrophages infiltration,which also raises the possibility that macrophages reduce ALOX15 levels in cervical cancer.Moreover,both univariate and multivariate Cox analyses show ALOX15 expression is independent prognostic factor and positively associated with good prognosis in cervical cancer.Altogether,this study reveals the potential utility of targeting TAMs in ferroptosis-based treatment and ALOX15 as prognosis indicators for cervical cancer.展开更多
A novel method has been developed to monitor the nucleic acids ligation process. Molecular beacon was employed here to convert the ligation information into fluo-rescence signal quickly and quantitatively. This method...A novel method has been developed to monitor the nucleic acids ligation process. Molecular beacon was employed here to convert the ligation information into fluo-rescence signal quickly and quantitatively. This method pro-vides effective and original approach to researching the dy-namic ligation process and the interactions between nucleic acids and ligase. An analytical method for T4 DNA ligase based on this way has been built up with a linear detection range from 2.3×10-4 U/mL to 0.23 U/mL. It is rapid and sensitive to detect 2.8×10-5 U T4 DNA ligase in 10 min.展开更多
文摘The ground-floor economy has a long history as a significant part of the informal economy.Due to the dependence on its own social status and relationship to the government’s political and economic objectives,it has developed precariously in recent years.In the face of post-epidemic problems,a shortcut is to learn from international experience.This paper used the structural theory and drew from the secondary data,demonstrating the background of informal economy and exploring the rational ways to maintain and develop street vending.Spatialization,legalization and network digitization are proven international approaches,which display the empirical and theoretical implications to urban practice and studies.
基金supported by the National Natural Science Foundation of China(Nos.62427803,62031018,and U23A20283)the Jiangsu Provincial Key Research and Development Program(No.BE2022391)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX24_0644)。
文摘Non-line-of-sight[NLOS]imaging enables the detection and reconstruction of hidden objects around corners,offering promising applications in autonomous driving,remote sensing,and medical diagnosis.However,existing steady-state NLOS imaging methods face challenges in achieving high efficiency and precision due to the need for multiple diffuse reflections and incomplete Fourier amplitude sampling.This study proposes,to our knowledge,a novel steady-state NLOS imaging technique via polarization differential correlography[PDC-NLOS].By employing the polarization difference of the laser speckle,the method designs a single-shot polarized speckle illumination strategy.The fast and stable real-time encoding for hidden objects ensures stable imaging quality of the PDC-NLOS system.The proposed method demonstrates millimeter-level imaging resolution when imaging horizontally and vertically striped objects.
基金National Natural Science Foundation of China(61971227,62031018,62101255)Jiangsu Provincial Key Research and Development Program(BE2022391)China Postdoctoral Science Foundation(2021M701721,2023T160319)。
文摘Non-line-of-sight(NLOS)imaging is a challenging task aimed at reconstructing objects outside the direct view of the observer.Nevertheless,traditional NLOS imaging methods typically rely on intricate and costly equipment to scan and sample the hidden object.These methods often suffer from restricted imaging resolution and require high system stability.Herein,we propose a single-shot high-resolution NLOS imaging method via chromato-axial differential correlography,which adopts low-cost continuous-wave lasers and a conventional camera.By leveraging the uncorrelated laser speckle patterns along the chromato-axis,this method can reconstruct hidden objects of diverse complexity using only one exposure measurement.The achieved background stability through singleshot acquisition,along with the inherent information redundancy in the chromato-axial differential speckles,enhances the robustness of the system against vibration and colored stain interference.This approach overcomes the limitations of conventional methods by simplifying the sampling process,improving system stability,and achieving enhanced imaging resolution using available equipment.This work serves as a valuable reference for the real-time development and practical implementation of NLOS imaging.
基金supported by the National Natural Science Foundation of China(81972479,U2004118,82072899,81772643 and 81772803)Natural Science Foundation of Guangdong province(2019A1515011100 and 2021A1515012576,China)+5 种基金Henan Natural Science Foundation(202300410359,China)Henan Medical Program(SBGJ2020002081,China)Guangzhou High-Level Clinical Key Specialty Construction ProjectClinical Key Specialty Construction Project of Guangzhou Medical University(202005,China)the Innovation Project of Universities in Guangdong Province(NO.2021KTSCX026,China)Funding for Scientific Research and Innovation Team of The First Affiliated Hospital of Zhengzhou University(QNCXTD2023019,China)。
文摘Induction of cancer cell ferroptosis has been proposed as a potential treatment in several cancer types.Tumor-associated macrophages(TAMs)play a key role in promoting tumor malignant progression and therapy resistance.However,the roles and mechanisms of TAMs in regulating tumor ferroptosis is still unexplored and remains enigmatic.This study shows ferroptosis inducers has shown therapeutic outcomes in cervical cancer in vitro and in vivo.TAMs have been found to suppress cervical cancer cells ferroptosis.Mechanistically,macrophage-derived miRNA-660-5p packaged into exosomes are transported into cancer cells.In cancer cells,miRNA-660-5p attenuates ALOX15 expression to inhibit ferroptosis.Moreover,the upregulation of miRNA-660-5p in macrophages depends on autocrine IL4/IL13-activated STAT6 pathway.Importantly,in clinical cervical cancer cases,ALOX15 is negatively associated with macrophages infiltration,which also raises the possibility that macrophages reduce ALOX15 levels in cervical cancer.Moreover,both univariate and multivariate Cox analyses show ALOX15 expression is independent prognostic factor and positively associated with good prognosis in cervical cancer.Altogether,this study reveals the potential utility of targeting TAMs in ferroptosis-based treatment and ALOX15 as prognosis indicators for cervical cancer.
文摘A novel method has been developed to monitor the nucleic acids ligation process. Molecular beacon was employed here to convert the ligation information into fluo-rescence signal quickly and quantitatively. This method pro-vides effective and original approach to researching the dy-namic ligation process and the interactions between nucleic acids and ligase. An analytical method for T4 DNA ligase based on this way has been built up with a linear detection range from 2.3×10-4 U/mL to 0.23 U/mL. It is rapid and sensitive to detect 2.8×10-5 U T4 DNA ligase in 10 min.
