Background:Sleep deprivation causes hippocampal injury,manifesting as neuronal damage and learning-memory impairment.These negative effects may be associated with disturbance of hippocampal glutamate and water homeost...Background:Sleep deprivation causes hippocampal injury,manifesting as neuronal damage and learning-memory impairment.These negative effects may be associated with disturbance of hippocampal glutamate and water homeostasis,which induces excessive neuronal excitability.Five percent CO_(2) inhalation has been shown to suppress neuronal excitability.Here,we aimed to investigate whether 5%CO_(2) inhalation facilitates the recovery of hippocampal glutamate and water homeostasis,neuron morphology,and learning-memory ability in sleep-deprived rats.Methods:Thirty-six Sprague-Dawley female rats were randomly divided into three groups including normal sleep(Group 1,NS,n=12),sleep deprivation followed by sleep recovery(Group 2,SD+SR,n=12),sleep deprivation followed by sleep recovery and 5%CO_(2) inhalation(Group 3,SD+SR+CO_(2),n=12)by random number table.Each group was divided into two subgroups(n=6 each subgroup)for different experiments randomly by random number table.Results:We found that 5%CO_(2) inhalation facilitated the recovery of hippocampal glutamate concentration(7.549±0.310,8.716±0.463,and 7.493±0.281 mmol/L at Days 1,3,and 5 in Group 3,F 2,15=22.06,p<0.0001)and hippocampal apparent diffusion coefficient mean value(8.210±0.274,7.685±0.171,8.265±0.269 at Days 1,3,and 5 in Group 3,F 2,15=10.45,p=0.0014),enhanced expression level of astrocyte-specific membrane protein glutamate transporter-1,promoted the polarized distribution of aquaporin 4,reduced hippocampal neuronal damage and improved learning-memory ability in sleep-deprived rats.Conclusion:This study showed that 5%CO_(2) inhalation can serve as a novel strategy for alleviating sleep deprivation-induced hippocampal injury.展开更多
基金National Natural Science Foundation of China,Grant/Award Numbers:31870981,82020108016Li Ka Shing Foundation,Grant/Award Numbers:2020LKSFG05D,2020LKSFG06CKey Disciplinary Projectof Clinical Medicine under the Guangdong High-Level University Development Program,Grant/Award Number:002-18120302。
文摘Background:Sleep deprivation causes hippocampal injury,manifesting as neuronal damage and learning-memory impairment.These negative effects may be associated with disturbance of hippocampal glutamate and water homeostasis,which induces excessive neuronal excitability.Five percent CO_(2) inhalation has been shown to suppress neuronal excitability.Here,we aimed to investigate whether 5%CO_(2) inhalation facilitates the recovery of hippocampal glutamate and water homeostasis,neuron morphology,and learning-memory ability in sleep-deprived rats.Methods:Thirty-six Sprague-Dawley female rats were randomly divided into three groups including normal sleep(Group 1,NS,n=12),sleep deprivation followed by sleep recovery(Group 2,SD+SR,n=12),sleep deprivation followed by sleep recovery and 5%CO_(2) inhalation(Group 3,SD+SR+CO_(2),n=12)by random number table.Each group was divided into two subgroups(n=6 each subgroup)for different experiments randomly by random number table.Results:We found that 5%CO_(2) inhalation facilitated the recovery of hippocampal glutamate concentration(7.549±0.310,8.716±0.463,and 7.493±0.281 mmol/L at Days 1,3,and 5 in Group 3,F 2,15=22.06,p<0.0001)and hippocampal apparent diffusion coefficient mean value(8.210±0.274,7.685±0.171,8.265±0.269 at Days 1,3,and 5 in Group 3,F 2,15=10.45,p=0.0014),enhanced expression level of astrocyte-specific membrane protein glutamate transporter-1,promoted the polarized distribution of aquaporin 4,reduced hippocampal neuronal damage and improved learning-memory ability in sleep-deprived rats.Conclusion:This study showed that 5%CO_(2) inhalation can serve as a novel strategy for alleviating sleep deprivation-induced hippocampal injury.
