Ground-based radar is the primary means by which severe storms are monitored and tracked;however, due to limited coverage, important data is often missed over ocean and mountainous areas. On the other hand, geostation...Ground-based radar is the primary means by which severe storms are monitored and tracked;however, due to limited coverage, important data is often missed over ocean and mountainous areas. On the other hand, geostationary(GEO)weather satellites provide continuous observations with seamless coverage with advanced imager, despite their limited capability to penetrate clouds. Combining satellite and ground-radar observations could exploit the advantages of both techniques, providing tracking capability close to that of ground radar while maintaining full spatial coverage. This study presents a novel method called Multi-dimensional satellite Observation information for Radar Estimation(MORE) to reconstruct radar composite reflectivity(CREF). Deep learning techniques are important components of MORE for estimating CREF from China's Fengyun-4B(FY-4B) GEO satellite observations. Two models are developed: an infraredonly(IR-Single) model available for all times, and a visible-infrared(VIS+IR) model for daytime applications. These models incorporate multi-dimensional satellite observation information, including temporal, spatial, spectral, and viewing angle information, to enhance the accuracy of radar echo reconstruction. Results demonstrate that the VIS+IR model outperforms the IR-Single model, and both models achieves a root-mean-square error(RMSE) of less than 6 dBZ and a coefficient of determination(R~2) of greater than 0.7. The models effectively reconstruct radar echoes, including strong echoes exceeding 50 dBZ, and show good agreement with precipitation data in radar-blind areas. This study offers a valuable solution for severe weather monitoring and tracking in regions lacking ground-based radar observations, and provides a potential tool for enhanced data assimilation in numerical weather prediction(NWP) models.展开更多
Attribute-Based Encryption(ABE)has emerged as a fundamental access control mechanism in data sharing,enabling data owners to define flexible access policies.A critical aspect of ABE is key revocation,which plays a piv...Attribute-Based Encryption(ABE)has emerged as a fundamental access control mechanism in data sharing,enabling data owners to define flexible access policies.A critical aspect of ABE is key revocation,which plays a pivotal role in maintaining security.However,existing key revocation mechanisms face two major challenges:(1)High overhead due to ciphertext and key updates,primarily stemming from the reliance on revocation lists during attribute revocation,which increases computation and communication costs.(2)Limited universality,as many attribute revocation mechanisms are tailored to specific ABE constructions,restricting their broader applicability.To address these challenges,we propose LUAR(Lightweight and Universal Attribute Revocation),a novel revocation mechanism that leverages Intel Software Guard Extensions(SGX)while minimizing its inherent limitations.Given SGX’s constrained memory(≈90 MB in a personal computer)and susceptibility to side-channel attacks,we carefully manage its usage to reduce reliance while mitigating potential collusion risks between cloud service providers and users.To evaluate LUAR’s lightweight and universality,we integrate it with the classic BSW07 scheme,which can be seamlessly replaced with other ABE constructions.Experimental results demonstrate that LUAR enables secure attribute revocation with low computation and communication overhead.The processing time within the SGX environment remains stable at approximately 55 ms,regardless of the complexity of access policies,ensuring no additional storage or computational burden on SGX.Compared to the Hardware-based Revocable Attribute-Based Encryption(HR-ABE)scheme(IEEE S&P 2024),LUAR incurs a slightly higher computational cost within SGX;however,the overall time from initiating a data request to obtaining plaintext is shorter.As access policies grow more complex,LUAR’s advantages become increasingly evident,showcasing its superior efficiency and broader applicability.展开更多
The variation of seedling characteristics under different water supply conditions is strongly associated with drought resistance in rice （Oryza sativa L.） and a better elucidation of its genetics is helpful for impr...The variation of seedling characteristics under different water supply conditions is strongly associated with drought resistance in rice （Oryza sativa L.） and a better elucidation of its genetics is helpful for improving rice drought resistance. Ninety-six doubled-haploid （DH） rice lines of an indica and japonica cross were grown in both flooding and upland conditions and QTLs for morphological traits at seedling stage were examined using 208 restriction fragment length polymorphism （RFLP） and 76 microsatellite （SSR） markers. A total of 32 putative QTLs were associated with the four seedling traits： average of three adventitious root lengths （ARL）, shoot height （SH）, shoot biomass （SW）, and root to shoot dry weight ratio （RSR）. Five QTLs detected were the same under control and upland conditions. The ratio between the mean value of the seedling trait under upland and flooding conditions was used for assessing drought tolerance. A total of six QTLs for drought tolerance were detected. Comparative analysis was performed for the QTLs detected in this case and those reported from two other populations with the same upland rice variety Azucena as parent. Several identical QTLs for seedling elongation across the three populations with the positive alleles from the upland rice Azucena were detected, which suggests that the alleles of Azucena might be involved in water stress-accelerated elongation of rice under different genetic backgrounds. Five cell wall-related candidate genes for OsEXPI, OsEXP2, OsEXP4, EXT, and EGase were mapped on the intervals carrying the QTLs for seedling traits.展开更多
The present study aimed to compare the complications and clinical outcomes of serial lumbar puncture（LP） and lumbar cerebrospinal fluid（CSF） drainage（LD） of patients with aneurysmal subarachnoid hemorrhage and p...The present study aimed to compare the complications and clinical outcomes of serial lumbar puncture（LP） and lumbar cerebrospinal fluid（CSF） drainage（LD） of patients with aneurysmal subarachnoid hemorrhage and provide more evidence to guide clinical management.In this retrospective study,41 and 39 aneurysmal subarachnoid hemorrhage patients were enrolled in the LP and LD group,respectively.Clinical outcomes,including CSF infection,intracerebral hemorrhage,vasospasm,hydrocephalus,death,length of stay,duration of drainage and the Glasgow Outcome Scale score were compared between the two groups.By comparing with the LP group,the LD group showed a significantly higher rate of CSF infection（P= 0.029） and shorter duration of drainage（P〈 0.001）.Both groups displayed similar rates of vasospasm,hydrocephalus,intracerebral hemorrhage,the Glasgow Outcome Scale score one month after endovascular coiling and length of stay（P〉 0.05,respectively）.In conclusion,both LD and serial LP are effective methods in the treatment of aneurysmal subarachnoid hemorrhage; besides,serial LP can reduce the incidence of CSF infection in draining hemorrhagic CSF in aneurysmal subarachnoid hemorrhage after endovascular coiling.展开更多
Determining the influences of environmental factors on seedling growth and leaf color may improve our understanding of the chromogenic mechanisms in leaves and the biosynthesis of anthocyanin. To determine the optimum...Determining the influences of environmental factors on seedling growth and leaf color may improve our understanding of the chromogenic mechanisms in leaves and the biosynthesis of anthocyanin. To determine the optimum conditions for Betula 'Royal Frost' seedling cultivation, we assessed seedling growth and anthocyanin content under natural conditions. The temperature had a significant influence on height but not on radial growth. Between June and September, the anthocyanin content was significantly positively correlated with light intensity and air moisture and significantly negatively correlated with temperature. In single-factor experiments to determine the variation in anthocyanin content in the greenhouse, anthocyanin content increased with increasing soil water content and at first increased, then decreased with increasing pH of the irrigation water and temperature, but decreased with increasing light intensity. K2SO4 can increase the anthocyanin content. So to maintain leaf color, seedlings should be cultivated with < 200 mu mol m(-2) s(-1) light intensity at 20 A degrees C and soil moisture content between 60 and 90%, and the irrigation water should be neutral or alkaline. Additionally, spraying with K2SO4 can be beneficial.展开更多
Surface-enhanced Raman spectroscopy(SERS), a powerful surface vibrational spectroscopic technique, is ideally suited for in situ monitoring the chemical transformations occurred at surfaces and/or interfaces.For in si...Surface-enhanced Raman spectroscopy(SERS), a powerful surface vibrational spectroscopic technique, is ideally suited for in situ monitoring the chemical transformations occurred at surfaces and/or interfaces.For in situ SERS monitoring, a platform integrated both plasmonic and catalytic activity is a prerequisite. Here, we fabricate a bifunctional Au-Pd nanocoronal film for in situ SERS monitoring Suzuki-Miyaura cross-coupling reaction. This excellent bifunctional substrate leads to the coupling of high catalytic activity with a strong SERS effect at the center of two adjacent Au cores and shows fine reproducibility and stability of SERS signals. During investigating the Suzuki reaction with in situ SERS, we found two distinct catalytic kinetic processes resulted from two disparate catalytic sites on a Au-Pd nanocoronal. Comparing with conventional analytical techniques, this work provides a novel approach for studying Suzuki reactions at surfaces and/or interfaces with in situ SERS.展开更多
We developed a shoot multiplication protocol for Syringa reticulata Blume vat. mandshurica Hara from in vitro cultured seedlings that derived from in vitro germinated seeds. The shoots could be induced on Murashige an...We developed a shoot multiplication protocol for Syringa reticulata Blume vat. mandshurica Hara from in vitro cultured seedlings that derived from in vitro germinated seeds. The shoots could be induced on Murashige and Skoog （MS） medium with proper plant growth regulator combinations of 6-benzylaminopurine （BA） and indole-3-butyric acid （IBA）. The better medium for shoot multiplication and growth was MS ＋ 5 mg L-1 BA ＋ 0.5 mg L-1 IBA ＋ 20 g L-1 sucrose ＋ 7 g L-1 agar, and the corresponding shoot induction rate was 75 %. The plantlets grew well after rooting on I/2MS medium （macro-elements of MS medium are at half-strength） sup- plemented with 1 mg L-1 IBA, and the survival percentage was 〉80 % at 16 weeks after transplanting.展开更多
We demonstrated an AlGaN/GaN high electron mobility transistor(HEMT)namely double-Vthcoupling HEMT(DVC-HEMT)fabricated by connecting different threshold voltage(Vth)values including the slant recess element and planar...We demonstrated an AlGaN/GaN high electron mobility transistor(HEMT)namely double-Vthcoupling HEMT(DVC-HEMT)fabricated by connecting different threshold voltage(Vth)values including the slant recess element and planar element in parallel along the gate width with N;O plasma treatment on the gate region.The comparative studies of DVC-HEMT and Fin-like HEMT fabricated on the same wafer show significantly improved linearity of transconductance(Gm)and radio frequency(RF)output signal characteristics in DVC-HEMT.The fabricated device shows the transconductance plateau larger than 7 V,which yields a flattened fT/fmax-gate bias dependence.At the operating frequency of 30 GHz,the peak power-added efficiency(PAE)of 41%accompanied by the power density(Pout)of 5.3 W/mm.Furthermore,the proposed architecture also features an exceptional linearity performance with 1-d B compression point(P1 d B)of 28 d Bm,whereas that of the Fin-like HEMT is 25.2 d Bm.The device demonstrated in this article has great potential to be a new paradigm for millimeter-wave application where high linearity is essential.展开更多
目的探讨24 h动态心电图联合骨膜蛋白(Periostin)、乳糖凝集素-3(Gal-3)对急性心肌梗死(AMI)后恶性心律失常(MA)的预测价值。方法选择2020年1月-2022年12月皖南医学院第一附属医院心血管内科收治的AMI患者367例为研究对象(AMI组),根据...目的探讨24 h动态心电图联合骨膜蛋白(Periostin)、乳糖凝集素-3(Gal-3)对急性心肌梗死(AMI)后恶性心律失常(MA)的预测价值。方法选择2020年1月-2022年12月皖南医学院第一附属医院心血管内科收治的AMI患者367例为研究对象(AMI组),根据是否发生MA分为非MA亚组(n=286)和MA亚组(n=81)。另选取医院同期健康体检志愿者370例为健康对照组。酶联免疫吸附试验(ELISA)检测血清Periostin、Gal-3水平;多因素Logistic回归分析AMI患者发生MA的影响因素;受试者工作特征(ROC)曲线分析Periostin、Gal-3、24 h QT间期变异性(24 h QTV)、24 h正常心房开始除极至心室开始除极时间间期标准差(SDNN)对AMI患者发生MA的预测价值。结果与健康对照组比较,AMI组血清Periostin、Gal-3水平均升高(t/P=59.225/<0.001、33.141/<0.001);与非MA亚组比较,MA亚组血清Periostin、Gal-3水平及低血钾比例均升高(t/χ^(2)/P=7.602/<0.001、6.049/<0.001、15.167/<0.001);与非MA亚组比较,MA亚组SDNN、24 h QTV、24 h连续5 min正常R-R间期标准差(SDANN-index)、24 h连续5 min正常R-R间期标准差均值(SDNN-index)水平降低(t/P=7.029/<0.001、7.401/<0.001、4.281/<0.001、4.721/<0.001);血清Periostin、Gal-3水平升高是AMI患者发生MA的危险因素[OR(95%CI)=2.163(1.416~3.303)、2.450(1.570~3.823)],24 h QTV、SDNN水平升高为保护因素[OR(95%CI)=0.449(0.264~0.764)、0.430(0.223~0.829)];24 h QTV、SDNN、Periostin、Gal-3单独及四项联合预测AMI患者发生MA的AUC分别为0.791、0.719、0.758、0.744、0.901,四者联合预测的AUC高于单独预测(Z/P=3.916/<0.001、6.101/<0.001、4.888/<0.001、5.764/<0.001)。结论AMI患者血清Periostin、Gal-3水平均升高,24 h动态心电图指标联合血清Periostin、Gal-3预测AMI患者发生MA的效能较高。展开更多
Andrographolide sulfonate(AS)is a sulfonated derivative of andrographolide extracted from Andrographis paniculata(Burm.f.)Nees,and has been approved for several decades in China.The present study aimed to investigate ...Andrographolide sulfonate(AS)is a sulfonated derivative of andrographolide extracted from Andrographis paniculata(Burm.f.)Nees,and has been approved for several decades in China.The present study aimed to investigate the novel therapeutic application and possible mechanisms of AS in the treatment of rheumatoid arthritis.Results indicated that administration of AS by injection or gavage significantly reduced the paw swelling,improved body weights,and attenuated pathological changes in joints of rats with adjuvant-induced arthritis.Additionally,the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),and IL-1β in the serum and ankle joints were reduced.Bioinformatics analysis,along with the spleen index and measurements of IL-17 and IL-10 levels,suggested a potential relationship between AS and Th17 cells under arthritic conditions.In vitro,AS was shown to block Th17 cell differentiation,as evidenced by the reduced percentages of CD4^(+)IL-17A^(+)T cells and decreased expression levels of RORγt,IL-17A,IL-17F,IL-21,and IL-22,without affecting the cell viability and apoptosis.This effect was attributed to the limited glycolysis,as indicated by metabolomics analysis,reduced glucose uptake,and p H measurements.Further investigation revealed that AS might bind to hexokinase2(HK2)to down-regulate the protein levels of HK2 but not glyceraldehyde-3-phosphate dehydrogenase(GAPDH)or pyruvate kinase M2(PKM2),and overexpression of HK2 reversed the inhibition of AS on Th17 cell differentiation.Furthermore,AS impaired the activation of phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signals in vivo and in vitro,which was abolished by the addition of lactate.In conclusion,AS significantly improved adjuvant-induced arthritis(AIA)in rats by inhibiting glycolysis-mediated activation of PI3K/AKT to restrain Th17 cell differentiation.展开更多
Pinus koraiensis(Sieb.et Zucc.) is a coniferous tree species naturally distributed in northeastern China.However,the effects of gene flow on its genetic diversity and structure remain unclear.This study investigates t...Pinus koraiensis(Sieb.et Zucc.) is a coniferous tree species naturally distributed in northeastern China.However,the effects of gene flow on its genetic diversity and structure remain unclear.This study investigates these dynamics in seven populations using ten microsatellite markers.The results show a high level of genetic diversity within the populations(Ho=0.633,He=0.746).In addition,molecular analysis of variance(AMOVA) shows that 98% of genetic diversity occurs within populations,with minimal differentiation between populations(Fst=0.009-0.033).Gene flow analysis shows significant migration rates between specific population pairs,particularly C-TH(87%),LS-Y(69%) and TH-LS(69%),suggesting genetic homogenization.Bayesian clustering(STRUCTURE) supports admixture and weak population differentiation.Environmental factors,especially temperature-related variables,significantly influence genetic patterns.Partial Mantel tests and multiple matrix regression show strong correlations between genetic distance and adaptations to cold temperatures(bio6 and bio11).Overall,this study emphasizes the robust genetic diversification and high migration rates in the populations of P.koraiensis and highlights their resilience.These results emphasize the importance of incorporating genetic and ecological factors into conservation strategies for sustainable forest management.This research provides valuable insights into the complex interplay of genetic variation,gene flow and environmental influences in forest tree species and improves our understanding of their adaptive mechanisms.展开更多
基金supported by the National Natural Science Foundation of China (NSFC) (Grant No.42205044)Feng Yun Application Pioneering Project (FY-APP) Innovation Center for Feng Yun Meteorological Satellite (FYSIC) Special Project (FY-APP-XC-2023.04)the Wuxi University Research Start-up Fund for Recruited Talent。
文摘Ground-based radar is the primary means by which severe storms are monitored and tracked;however, due to limited coverage, important data is often missed over ocean and mountainous areas. On the other hand, geostationary(GEO)weather satellites provide continuous observations with seamless coverage with advanced imager, despite their limited capability to penetrate clouds. Combining satellite and ground-radar observations could exploit the advantages of both techniques, providing tracking capability close to that of ground radar while maintaining full spatial coverage. This study presents a novel method called Multi-dimensional satellite Observation information for Radar Estimation(MORE) to reconstruct radar composite reflectivity(CREF). Deep learning techniques are important components of MORE for estimating CREF from China's Fengyun-4B(FY-4B) GEO satellite observations. Two models are developed: an infraredonly(IR-Single) model available for all times, and a visible-infrared(VIS+IR) model for daytime applications. These models incorporate multi-dimensional satellite observation information, including temporal, spatial, spectral, and viewing angle information, to enhance the accuracy of radar echo reconstruction. Results demonstrate that the VIS+IR model outperforms the IR-Single model, and both models achieves a root-mean-square error(RMSE) of less than 6 dBZ and a coefficient of determination(R~2) of greater than 0.7. The models effectively reconstruct radar echoes, including strong echoes exceeding 50 dBZ, and show good agreement with precipitation data in radar-blind areas. This study offers a valuable solution for severe weather monitoring and tracking in regions lacking ground-based radar observations, and provides a potential tool for enhanced data assimilation in numerical weather prediction(NWP) models.
基金support from the National Key Research and Development Program of China(Grant No.2021YFF0704102)the Chongqing Education Commission Key Project of Science and Technology Research(Grant No.KJZD-K202400610)the Chongqing Natural Science Foundation General Project(Grant No.CSTB2025NSCQ-GPX1263).
文摘Attribute-Based Encryption(ABE)has emerged as a fundamental access control mechanism in data sharing,enabling data owners to define flexible access policies.A critical aspect of ABE is key revocation,which plays a pivotal role in maintaining security.However,existing key revocation mechanisms face two major challenges:(1)High overhead due to ciphertext and key updates,primarily stemming from the reliance on revocation lists during attribute revocation,which increases computation and communication costs.(2)Limited universality,as many attribute revocation mechanisms are tailored to specific ABE constructions,restricting their broader applicability.To address these challenges,we propose LUAR(Lightweight and Universal Attribute Revocation),a novel revocation mechanism that leverages Intel Software Guard Extensions(SGX)while minimizing its inherent limitations.Given SGX’s constrained memory(≈90 MB in a personal computer)and susceptibility to side-channel attacks,we carefully manage its usage to reduce reliance while mitigating potential collusion risks between cloud service providers and users.To evaluate LUAR’s lightweight and universality,we integrate it with the classic BSW07 scheme,which can be seamlessly replaced with other ABE constructions.Experimental results demonstrate that LUAR enables secure attribute revocation with low computation and communication overhead.The processing time within the SGX environment remains stable at approximately 55 ms,regardless of the complexity of access policies,ensuring no additional storage or computational burden on SGX.Compared to the Hardware-based Revocable Attribute-Based Encryption(HR-ABE)scheme(IEEE S&P 2024),LUAR incurs a slightly higher computational cost within SGX;however,the overall time from initiating a data request to obtaining plaintext is shorter.As access policies grow more complex,LUAR’s advantages become increasingly evident,showcasing its superior efficiency and broader applicability.
基金the National Key Basic Research Special Foundation of China (No. G1999011700) Zhejiang Provincial Natural Science Foundation of China (No. Y305314).
文摘The variation of seedling characteristics under different water supply conditions is strongly associated with drought resistance in rice （Oryza sativa L.） and a better elucidation of its genetics is helpful for improving rice drought resistance. Ninety-six doubled-haploid （DH） rice lines of an indica and japonica cross were grown in both flooding and upland conditions and QTLs for morphological traits at seedling stage were examined using 208 restriction fragment length polymorphism （RFLP） and 76 microsatellite （SSR） markers. A total of 32 putative QTLs were associated with the four seedling traits： average of three adventitious root lengths （ARL）, shoot height （SH）, shoot biomass （SW）, and root to shoot dry weight ratio （RSR）. Five QTLs detected were the same under control and upland conditions. The ratio between the mean value of the seedling trait under upland and flooding conditions was used for assessing drought tolerance. A total of six QTLs for drought tolerance were detected. Comparative analysis was performed for the QTLs detected in this case and those reported from two other populations with the same upland rice variety Azucena as parent. Several identical QTLs for seedling elongation across the three populations with the positive alleles from the upland rice Azucena were detected, which suggests that the alleles of Azucena might be involved in water stress-accelerated elongation of rice under different genetic backgrounds. Five cell wall-related candidate genes for OsEXPI, OsEXP2, OsEXP4, EXT, and EGase were mapped on the intervals carrying the QTLs for seedling traits.
文摘The present study aimed to compare the complications and clinical outcomes of serial lumbar puncture（LP） and lumbar cerebrospinal fluid（CSF） drainage（LD） of patients with aneurysmal subarachnoid hemorrhage and provide more evidence to guide clinical management.In this retrospective study,41 and 39 aneurysmal subarachnoid hemorrhage patients were enrolled in the LP and LD group,respectively.Clinical outcomes,including CSF infection,intracerebral hemorrhage,vasospasm,hydrocephalus,death,length of stay,duration of drainage and the Glasgow Outcome Scale score were compared between the two groups.By comparing with the LP group,the LD group showed a significantly higher rate of CSF infection（P= 0.029） and shorter duration of drainage（P〈 0.001）.Both groups displayed similar rates of vasospasm,hydrocephalus,intracerebral hemorrhage,the Glasgow Outcome Scale score one month after endovascular coiling and length of stay（P〉 0.05,respectively）.In conclusion,both LD and serial LP are effective methods in the treatment of aneurysmal subarachnoid hemorrhage; besides,serial LP can reduce the incidence of CSF infection in draining hemorrhagic CSF in aneurysmal subarachnoid hemorrhage after endovascular coiling.
基金supported by the Postdoctoral Science Foundation of Heilongjiang Province of China(LBHQ14003)the National Natural Science Foundation of China(No.31400535)
文摘Determining the influences of environmental factors on seedling growth and leaf color may improve our understanding of the chromogenic mechanisms in leaves and the biosynthesis of anthocyanin. To determine the optimum conditions for Betula 'Royal Frost' seedling cultivation, we assessed seedling growth and anthocyanin content under natural conditions. The temperature had a significant influence on height but not on radial growth. Between June and September, the anthocyanin content was significantly positively correlated with light intensity and air moisture and significantly negatively correlated with temperature. In single-factor experiments to determine the variation in anthocyanin content in the greenhouse, anthocyanin content increased with increasing soil water content and at first increased, then decreased with increasing pH of the irrigation water and temperature, but decreased with increasing light intensity. K2SO4 can increase the anthocyanin content. So to maintain leaf color, seedlings should be cultivated with < 200 mu mol m(-2) s(-1) light intensity at 20 A degrees C and soil moisture content between 60 and 90%, and the irrigation water should be neutral or alkaline. Additionally, spraying with K2SO4 can be beneficial.
基金the financial support from the National Natural Science Foundation of China (No. 22022406)the Natural Science Foundation of Tianjin (Nos. 20JCJQJC00110 and 20JCYBJC00590)+1 种基金the 111 project (No. B12015)the Haihe Laboratory of Sustainable Chemical Transformations。
文摘Surface-enhanced Raman spectroscopy(SERS), a powerful surface vibrational spectroscopic technique, is ideally suited for in situ monitoring the chemical transformations occurred at surfaces and/or interfaces.For in situ SERS monitoring, a platform integrated both plasmonic and catalytic activity is a prerequisite. Here, we fabricate a bifunctional Au-Pd nanocoronal film for in situ SERS monitoring Suzuki-Miyaura cross-coupling reaction. This excellent bifunctional substrate leads to the coupling of high catalytic activity with a strong SERS effect at the center of two adjacent Au cores and shows fine reproducibility and stability of SERS signals. During investigating the Suzuki reaction with in situ SERS, we found two distinct catalytic kinetic processes resulted from two disparate catalytic sites on a Au-Pd nanocoronal. Comparing with conventional analytical techniques, this work provides a novel approach for studying Suzuki reactions at surfaces and/or interfaces with in situ SERS.
基金supported by the Special Funding for Distinguished Researchers by Northeast Forestry University,China
文摘We developed a shoot multiplication protocol for Syringa reticulata Blume vat. mandshurica Hara from in vitro cultured seedlings that derived from in vitro germinated seeds. The shoots could be induced on Murashige and Skoog （MS） medium with proper plant growth regulator combinations of 6-benzylaminopurine （BA） and indole-3-butyric acid （IBA）. The better medium for shoot multiplication and growth was MS ＋ 5 mg L-1 BA ＋ 0.5 mg L-1 IBA ＋ 20 g L-1 sucrose ＋ 7 g L-1 agar, and the corresponding shoot induction rate was 75 %. The plantlets grew well after rooting on I/2MS medium （macro-elements of MS medium are at half-strength） sup- plemented with 1 mg L-1 IBA, and the survival percentage was 〉80 % at 16 weeks after transplanting.
基金supported by the National Key Research and Development Program of China(Grant No.2020YFB1804902)the Fundamental Research Funds for the Central Universities+3 种基金the Innovation Fund of Xidian Universitythe National Natural Science Foundation of China(Grant No.61904135)the China Postdoctoral Science Foundation(Grant Nos.2018M640957 and BX20200262)the Research and Development Plan of Key Fields in Guangzhou(Grant No.202103020002)。
文摘We demonstrated an AlGaN/GaN high electron mobility transistor(HEMT)namely double-Vthcoupling HEMT(DVC-HEMT)fabricated by connecting different threshold voltage(Vth)values including the slant recess element and planar element in parallel along the gate width with N;O plasma treatment on the gate region.The comparative studies of DVC-HEMT and Fin-like HEMT fabricated on the same wafer show significantly improved linearity of transconductance(Gm)and radio frequency(RF)output signal characteristics in DVC-HEMT.The fabricated device shows the transconductance plateau larger than 7 V,which yields a flattened fT/fmax-gate bias dependence.At the operating frequency of 30 GHz,the peak power-added efficiency(PAE)of 41%accompanied by the power density(Pout)of 5.3 W/mm.Furthermore,the proposed architecture also features an exceptional linearity performance with 1-d B compression point(P1 d B)of 28 d Bm,whereas that of the Fin-like HEMT is 25.2 d Bm.The device demonstrated in this article has great potential to be a new paradigm for millimeter-wave application where high linearity is essential.
文摘目的探讨24 h动态心电图联合骨膜蛋白(Periostin)、乳糖凝集素-3(Gal-3)对急性心肌梗死(AMI)后恶性心律失常(MA)的预测价值。方法选择2020年1月-2022年12月皖南医学院第一附属医院心血管内科收治的AMI患者367例为研究对象(AMI组),根据是否发生MA分为非MA亚组(n=286)和MA亚组(n=81)。另选取医院同期健康体检志愿者370例为健康对照组。酶联免疫吸附试验(ELISA)检测血清Periostin、Gal-3水平;多因素Logistic回归分析AMI患者发生MA的影响因素;受试者工作特征(ROC)曲线分析Periostin、Gal-3、24 h QT间期变异性(24 h QTV)、24 h正常心房开始除极至心室开始除极时间间期标准差(SDNN)对AMI患者发生MA的预测价值。结果与健康对照组比较,AMI组血清Periostin、Gal-3水平均升高(t/P=59.225/<0.001、33.141/<0.001);与非MA亚组比较,MA亚组血清Periostin、Gal-3水平及低血钾比例均升高(t/χ^(2)/P=7.602/<0.001、6.049/<0.001、15.167/<0.001);与非MA亚组比较,MA亚组SDNN、24 h QTV、24 h连续5 min正常R-R间期标准差(SDANN-index)、24 h连续5 min正常R-R间期标准差均值(SDNN-index)水平降低(t/P=7.029/<0.001、7.401/<0.001、4.281/<0.001、4.721/<0.001);血清Periostin、Gal-3水平升高是AMI患者发生MA的危险因素[OR(95%CI)=2.163(1.416~3.303)、2.450(1.570~3.823)],24 h QTV、SDNN水平升高为保护因素[OR(95%CI)=0.449(0.264~0.764)、0.430(0.223~0.829)];24 h QTV、SDNN、Periostin、Gal-3单独及四项联合预测AMI患者发生MA的AUC分别为0.791、0.719、0.758、0.744、0.901,四者联合预测的AUC高于单独预测(Z/P=3.916/<0.001、6.101/<0.001、4.888/<0.001、5.764/<0.001)。结论AMI患者血清Periostin、Gal-3水平均升高,24 h动态心电图指标联合血清Periostin、Gal-3预测AMI患者发生MA的效能较高。
基金supported by the project of Central Funds Guiding the Local Science and Technology Development(No.20212ZDD02010)。
文摘Andrographolide sulfonate(AS)is a sulfonated derivative of andrographolide extracted from Andrographis paniculata(Burm.f.)Nees,and has been approved for several decades in China.The present study aimed to investigate the novel therapeutic application and possible mechanisms of AS in the treatment of rheumatoid arthritis.Results indicated that administration of AS by injection or gavage significantly reduced the paw swelling,improved body weights,and attenuated pathological changes in joints of rats with adjuvant-induced arthritis.Additionally,the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),and IL-1β in the serum and ankle joints were reduced.Bioinformatics analysis,along with the spleen index and measurements of IL-17 and IL-10 levels,suggested a potential relationship between AS and Th17 cells under arthritic conditions.In vitro,AS was shown to block Th17 cell differentiation,as evidenced by the reduced percentages of CD4^(+)IL-17A^(+)T cells and decreased expression levels of RORγt,IL-17A,IL-17F,IL-21,and IL-22,without affecting the cell viability and apoptosis.This effect was attributed to the limited glycolysis,as indicated by metabolomics analysis,reduced glucose uptake,and p H measurements.Further investigation revealed that AS might bind to hexokinase2(HK2)to down-regulate the protein levels of HK2 but not glyceraldehyde-3-phosphate dehydrogenase(GAPDH)or pyruvate kinase M2(PKM2),and overexpression of HK2 reversed the inhibition of AS on Th17 cell differentiation.Furthermore,AS impaired the activation of phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signals in vivo and in vitro,which was abolished by the addition of lactate.In conclusion,AS significantly improved adjuvant-induced arthritis(AIA)in rats by inhibiting glycolysis-mediated activation of PI3K/AKT to restrain Th17 cell differentiation.
基金funded by grants from the Innovation Project of the State Key Laboratory of Tree Genetics and Breeding(2021B01).
文摘Pinus koraiensis(Sieb.et Zucc.) is a coniferous tree species naturally distributed in northeastern China.However,the effects of gene flow on its genetic diversity and structure remain unclear.This study investigates these dynamics in seven populations using ten microsatellite markers.The results show a high level of genetic diversity within the populations(Ho=0.633,He=0.746).In addition,molecular analysis of variance(AMOVA) shows that 98% of genetic diversity occurs within populations,with minimal differentiation between populations(Fst=0.009-0.033).Gene flow analysis shows significant migration rates between specific population pairs,particularly C-TH(87%),LS-Y(69%) and TH-LS(69%),suggesting genetic homogenization.Bayesian clustering(STRUCTURE) supports admixture and weak population differentiation.Environmental factors,especially temperature-related variables,significantly influence genetic patterns.Partial Mantel tests and multiple matrix regression show strong correlations between genetic distance and adaptations to cold temperatures(bio6 and bio11).Overall,this study emphasizes the robust genetic diversification and high migration rates in the populations of P.koraiensis and highlights their resilience.These results emphasize the importance of incorporating genetic and ecological factors into conservation strategies for sustainable forest management.This research provides valuable insights into the complex interplay of genetic variation,gene flow and environmental influences in forest tree species and improves our understanding of their adaptive mechanisms.
