Cross contaminations easily occur from deteriorated food products to food contact surfaces,leading to the contamination of multiple lots of food in the transport package.Therefore,food packaging,particularly transport...Cross contaminations easily occur from deteriorated food products to food contact surfaces,leading to the contamination of multiple lots of food in the transport package.Therefore,food packaging,particularly transport packaging with efficient antimicrobial activity and rechargeability is highly demanded.In this study,a bio-based antimicrobial film was developed by incorporating the food-grade additiveε-poly-L-lysine(EPL)(antimicrobial)into film matrix,stabilized with phase-transitioned lysozyme(PTL).PTL enhanced chlorine binding to the film surface,improving its stability in various food simulants.Notably,even after 10 discharge and recharge cycles,the film maintained superior antimicrobial efficacy compared to the controls.Moreover,the combination of PTL and EPL showed a synergistic antimicrobial effect,inactivating 6 log CFU/mL of Staphylococcus aureus and Escherichia coli after only 30 min of exposure.The film displayed superior transparency,hydrophilicity,and robust mechanical strength.Pilot study further proved the preservative effects of modified coatings on fresh blueberries in terms of rot rate.展开更多
基金supported by the Key Projects of International Cooperation and Exchanges of Shaanxi Province(2023-GHZD-53)National Natural Science Foundation of China(32001630)Xi’an Agricultural Technology Research and Development Project(24NYGG0110)。
文摘Cross contaminations easily occur from deteriorated food products to food contact surfaces,leading to the contamination of multiple lots of food in the transport package.Therefore,food packaging,particularly transport packaging with efficient antimicrobial activity and rechargeability is highly demanded.In this study,a bio-based antimicrobial film was developed by incorporating the food-grade additiveε-poly-L-lysine(EPL)(antimicrobial)into film matrix,stabilized with phase-transitioned lysozyme(PTL).PTL enhanced chlorine binding to the film surface,improving its stability in various food simulants.Notably,even after 10 discharge and recharge cycles,the film maintained superior antimicrobial efficacy compared to the controls.Moreover,the combination of PTL and EPL showed a synergistic antimicrobial effect,inactivating 6 log CFU/mL of Staphylococcus aureus and Escherichia coli after only 30 min of exposure.The film displayed superior transparency,hydrophilicity,and robust mechanical strength.Pilot study further proved the preservative effects of modified coatings on fresh blueberries in terms of rot rate.