Despite the widespread presence and frequent detection of polycyclic aromatic hydrocarbons(PAHs)in various aspects of life,there is limited research on their exposure levels in pregnant women and cumulative exposure f...Despite the widespread presence and frequent detection of polycyclic aromatic hydrocarbons(PAHs)in various aspects of life,there is limited research on their exposure levels in pregnant women and cumulative exposure from the living environment.This study included 1311 women in late pregnancy from the Zunyi birth cohort and measured the urinary concentrations of 10 hydroxylated PAH metabolites(OH-PAHs).Risk assessment was conducted based on the estimated daily intake to calculate the hazard quotient and hazard index(HI).A linear regression model was used to analyze the relationship between creatinine-adjusted OH-PAHs concentrations and living environment and lifestyle factors,while principal component analysis was applied to trace the sources of PAHs exposure.1-OHPYR was detected in all participants’urine,with naphthalene metabolites having the highest concentrations among creatinine-adjusted PAHs.OH-PAHs concentrations were associated with housing type,room number,cooking frequency,household size,exercise frequency,fuel type,distance from main road,and drinking water source.Pregnant women using traditional fuels and living in bungalows had higher health risks than those using clean energy and living in buildings.Those living within 100 m of a main road had higher HI than those farther away.Coal combustion was identified as the primary source of PAHs exposure.The study emphasizes the importance of reducing PAHs exposure,especially for pregnant women living in polluted environments.It recommends public health interventions such as improving indoor ventilation and providing clean energy to reduce related health risks.展开更多
Objective:To analyze the expression and clinical significance of the zinc finger protein ZNF207 gene in liver hepatocellular carcinoma(LIHC)based on The Cancer Genome Atlas(TCGA)database.Methods:The mRNA sequencing da...Objective:To analyze the expression and clinical significance of the zinc finger protein ZNF207 gene in liver hepatocellular carcinoma(LIHC)based on The Cancer Genome Atlas(TCGA)database.Methods:The mRNA sequencing data of 371 cases of primary liver cancer,50 cases of normal tissues,and 3 cases of recurrent liver cancer were downloaded from the TCGA database.The corresponding clinical information of the 371 cases of hepatocellular carcinoma was subsequently analyzed.The difference in ZNF207 expression between normal and tumor tissues was analyzed using the UALCAN online database.The impact of ZNF207 expression on survival prognosis was assessed using the Kaplan-Meier method in R software.The GO and KEGG pathways of ZNF207 were analyzed.The Cox proportional hazards model was used to evaluate the prognostic factors of patients with LIHC.RT-qPCR was employed to verify the expression of ZNF207 in LIHC cells.Results:ZNF207 was highly expressed in LIHC tissues and HepG2 cells,with a significant difference(P<0.05).Multivariate Cox regression analysis revealed that patients with high ZNF207 expression had a significantly shorter overall survival time compared to those with low ZNF207 expression(HR=1.466,95%CI:1.011-2.126,P<0.05).GO enrichment analysis suggested that ZNF207 may influence the onset and progression of hepatocellular carcinoma by regulating mRNA splicing and mRNA transcription processing through the spliceosome.KEGG pathway enrichment analysis indicated that ZNF207 might affect the onset and progression of hepatocellular carcinoma through mitophagy,mRNA surveillance,homologous recombination,spliceosome,and nuclear-cytoplasmic transport.Conclusion:The expression of ZNF207 may be an independent predictor of the prognosis of patients with LIHC and could influence the development of hepatocellular carcinoma through various gene functions and pathways.It has the potential to serve as a novel molecular marker for predicting the prognosis of hepatocellular carcinoma.展开更多
基金supported by the National Key R&D Program of China(Nos.2018YFC1004300 and 2018YFC1004302)the Science&Technology Program of Guizhou Province(Nos.QKHHBZ[2020]3002,QKHPTRC-GCC[2022]039-1 and QKHPTRCCXTD[2022]014)the Scientific Research Program of Guizhou Provincial Department of Education(No.QJJ[2023]019).
文摘Despite the widespread presence and frequent detection of polycyclic aromatic hydrocarbons(PAHs)in various aspects of life,there is limited research on their exposure levels in pregnant women and cumulative exposure from the living environment.This study included 1311 women in late pregnancy from the Zunyi birth cohort and measured the urinary concentrations of 10 hydroxylated PAH metabolites(OH-PAHs).Risk assessment was conducted based on the estimated daily intake to calculate the hazard quotient and hazard index(HI).A linear regression model was used to analyze the relationship between creatinine-adjusted OH-PAHs concentrations and living environment and lifestyle factors,while principal component analysis was applied to trace the sources of PAHs exposure.1-OHPYR was detected in all participants’urine,with naphthalene metabolites having the highest concentrations among creatinine-adjusted PAHs.OH-PAHs concentrations were associated with housing type,room number,cooking frequency,household size,exercise frequency,fuel type,distance from main road,and drinking water source.Pregnant women using traditional fuels and living in bungalows had higher health risks than those using clean energy and living in buildings.Those living within 100 m of a main road had higher HI than those farther away.Coal combustion was identified as the primary source of PAHs exposure.The study emphasizes the importance of reducing PAHs exposure,especially for pregnant women living in polluted environments.It recommends public health interventions such as improving indoor ventilation and providing clean energy to reduce related health risks.
文摘Objective:To analyze the expression and clinical significance of the zinc finger protein ZNF207 gene in liver hepatocellular carcinoma(LIHC)based on The Cancer Genome Atlas(TCGA)database.Methods:The mRNA sequencing data of 371 cases of primary liver cancer,50 cases of normal tissues,and 3 cases of recurrent liver cancer were downloaded from the TCGA database.The corresponding clinical information of the 371 cases of hepatocellular carcinoma was subsequently analyzed.The difference in ZNF207 expression between normal and tumor tissues was analyzed using the UALCAN online database.The impact of ZNF207 expression on survival prognosis was assessed using the Kaplan-Meier method in R software.The GO and KEGG pathways of ZNF207 were analyzed.The Cox proportional hazards model was used to evaluate the prognostic factors of patients with LIHC.RT-qPCR was employed to verify the expression of ZNF207 in LIHC cells.Results:ZNF207 was highly expressed in LIHC tissues and HepG2 cells,with a significant difference(P<0.05).Multivariate Cox regression analysis revealed that patients with high ZNF207 expression had a significantly shorter overall survival time compared to those with low ZNF207 expression(HR=1.466,95%CI:1.011-2.126,P<0.05).GO enrichment analysis suggested that ZNF207 may influence the onset and progression of hepatocellular carcinoma by regulating mRNA splicing and mRNA transcription processing through the spliceosome.KEGG pathway enrichment analysis indicated that ZNF207 might affect the onset and progression of hepatocellular carcinoma through mitophagy,mRNA surveillance,homologous recombination,spliceosome,and nuclear-cytoplasmic transport.Conclusion:The expression of ZNF207 may be an independent predictor of the prognosis of patients with LIHC and could influence the development of hepatocellular carcinoma through various gene functions and pathways.It has the potential to serve as a novel molecular marker for predicting the prognosis of hepatocellular carcinoma.
